Restriction map for
pF1KB6564
(Circular) (Six-base) MAPSORT of: pF1KB6564.seq Check: 5149 from: 1 to: 4313
pF1KB6564 4313 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 959 1351 959
Size: 392 3921
Acc65I G'GTAC_C
Cuts at: 899 1329 899
Size: 430 3883
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2138 2570 3317 3585 1207
Size: 931 432 747 268 1935
Fragments arranged by size:
1935 931 747 432 268
AflIII A'CryG_T
Cuts at: 1665 2769 3746 1665
Size: 1104 977 2232
Fragments arranged by size:
2232 1104 977
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AleI CACnn'nnGTG
Cuts at: 1303 1303
Size: 4313
AlwNI CAG_nnn'CTG
Cuts at: 471 582 1618 3185 471
Size: 111 1036 1567 1599
Fragments arranged by size:
1599 1567 1036 111
ApaI G_GGCC'C
Cuts at: 172 172
Size: 4313
ApaLI G'TGCA_C
Cuts at: 3083 3083
Size: 4313
ApoI r'AATT_y
Cuts at: 1317 3666 3922 1317
Size: 2349 256 1708
Fragments arranged by size:
2349 1708 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 387 604 1009 1333 387
Size: 217 405 324 3367
Fragments arranged by size:
3367 405 324 217
BamHI G'GATC_C
Cuts at: 1338 1338
Size: 4313
BanI G'GyrC_C
Cuts at: 190 899 1013 1329 1743 1991 2026 190
Size: 709 114 316 414 248 35 2477
Fragments arranged by size:
2477 709 414 316 248 114 35
BanII G_rGCy'C
Cuts at: 172 1327 2357 172
Size: 1155 1030 2128
Fragments arranged by size:
2128 1155 1030
BbeI G_GCGC'C
Cuts at: 194 1017 1747 1995 194
Size: 823 730 248 2512
Fragments arranged by size:
2512 823 730 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4279
BciVI GTATCCnnnnn_n'
Cuts at: 2211 2972 4313 2211
Size: 761 1341 2211
Fragments arranged by size:
2211 1341 761
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BglI GCCn_nnn'nGGC
Cuts at: 112 199 1011 112
Size: 87 812 3414
Fragments arranged by size:
3414 812 87
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 1425 1425
Size: 4313
Bme1580I G_kGCm'C
Cuts at: 172 796 832 1938 2031 3087 172
Size: 624 36 1106 93 1056 1398
Fragments arranged by size:
1398 1106 1056 624 93 36
BmrI ACTGGGnnnn_n'
Cuts at: 1525 1606 1941 4085 1525
Size: 81 335 2144 1753
Fragments arranged by size:
2144 1753 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 210 2680 210
Size: 2470 1843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 879 952 250
Size: 629 73 3611
Fragments arranged by size:
3611 629 73
Bpu10I CC'TnA_GC
Cuts at: 1100 1100
Size: 4313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1489 2368 2860 3158 442
Size: 1047 879 492 298 1597
Fragments arranged by size:
1597 1047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4017 4017
Size: 4313
BsaAI yAC'GTr
Cuts at: 1666 1666
Size: 4313
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 191 920 1014 1744 1992 2694 4190 191
Size: 729 94 730 248 702 1496 314
Fragments arranged by size:
1496 730 729 702 314 248 94
BsaWI w'CCGG_w
Cuts at: 440 813 1502 2023 2701 2975 3122 3533 440
Size: 373 689 521 678 274 147 411 1220
Fragments arranged by size:
1220 689 678 521 411 373 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 550 580 550
Size: 30 4283
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 622 247
Size: 375 3938
BseYI C'CCAG_C
Cuts at: 339 1167 1738 2496 3073 3569 339
Size: 828 571 758 577 496 1083
Fragments arranged by size:
1083 828 758 577 571 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 865 457
Size: 408 3905
BsiEI CG_ry'CG
Cuts at: 104 1000 1855 1901 3109 104
Size: 896 855 46 1208 1308
Fragments arranged by size:
1308 1208 896 855 46
BsiHKAI G_wGCw'C
Cuts at: 1327 2105 2295 3087 1327
Size: 778 190 792 2553
Fragments arranged by size:
2553 792 778 190
BsmI GAATG_Cn'
Cuts at: 484 484
Size: 4313
Bsp1286I G_dGCh'C
Cuts at: 172 796 832 1327 1938 2031 2105 2295 2357
Size: 624 36 495 611 93 74 190 62
Cuts at: 2357 3087 172
Size: 730 1398
Fragments arranged by size:
1398 730 624 611 495 190 93 74
62 36
BspEI T'CCGG_A
Cuts at: 440 813 3533 440
Size: 373 2720 1220
Fragments arranged by size:
2720 1220 373
BspHI T'CATG_A
Cuts at: 3489 3489
Size: 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 224 456 1363 1577 1879 2260 224
Size: 232 907 214 302 381 2277
Fragments arranged by size:
2277 907 381 302 232 214
BsrBI CCG'CTC
Cuts at: 50 755 819 1176 2605 2659 4143 4308 50
Size: 705 64 357 1429 54 1484 165 55
Fragments arranged by size:
1484 1429 705 357 165 64 55 54
BsrDI GCAATG_nn'
Cuts at: 2225 2225
Size: 4313
BsrFI r'CCGG_y
Cuts at: 126 135 432 947 1043 1246 2311 2492 2701
Size: 9 297 515 96 203 1065 181 209
Cuts at: 2701 126
Size: 1738
Fragments arranged by size:
1738 1065 515 297 209 203 181 96
9
BssSI C'ACGA_G
Cuts at: 709 2584 2942 709
Size: 1875 358 2080
Fragments arranged by size:
2080 1875 358
BstAPI GCAn_nnn'nTGC
Cuts at: 471 1690 471
Size: 1219 3094
BstBI TT'CG_AA
Cuts at: 323 2674 323
Size: 2351 1962
BstEII G'GTnAC_C
Cuts at: 363 363
Size: 4313
BstXI CCAn_nnnn'nTGG
Cuts at: 930 930
Size: 4313
BstYI r'GATC_y
Cuts at: 1 329 611 1338 1805 1828 2163 2409 3421
Size: 328 282 727 467 23 335 246 1012
Cuts at: 3421 3507 3519 3865 1
Size: 86 12 346 449
Fragments arranged by size:
1012 727 467 449 346 335 328 282
246 86 23 12
BtgI C'CryG_G
Cuts at: 773 953 1052 1304 773
Size: 180 99 252 3782
Fragments arranged by size:
3782 252 180 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 144 615 2263 2444 2607 144
Size: 471 1648 181 163 1850
Fragments arranged by size:
1850 1648 471 181 163
BtsI GCAGTG_nn'
Cuts at: 1556 1556
Size: 4313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 191 226 191
Size: 35 4278
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 2019 2877 2019
Size: 858 3455
EaeI y'GGCC_r
Cuts at: 401 434 756 776 887 945 997 1055 1898
Size: 33 322 20 111 58 52 58 843
Cuts at: 1898 2072 2463 2490 3835 401
Size: 174 391 27 1345 879
Fragments arranged by size:
1345 879 843 391 322 174 111 58
58 52 33 27 20
EagI C'GGCC_G
Cuts at: 997 1898 997
Size: 901 3412
EarI CTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1195 2521 2831 2977 4123 1195
Size: 1326 310 146 1146 1385
Fragments arranged by size:
1385 1326 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1325 1325
Size: 4313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 879 952 1207 2138 2570 3317 3585 250
Size: 629 73 255 931 432 747 268 978
Fragments arranged by size:
978 931 747 629 432 268 255 73
EcoNI CCTnn'n_nnAGG
Cuts at: 574 574
Size: 4313
EcoO109I rG'GnC_Cy
Cuts at: 168 468 512 1004 1452 168
Size: 300 44 492 448 3029
Fragments arranged by size:
3029 492 448 300 44
EcoRI G'AATT_C
Cuts at: 1317 1317
Size: 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FseI GG_CCGG'CC
Cuts at: 951 951
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 142 194 1017 1747 1995 3017 3996 142
Size: 52 823 730 248 1022 979 459
Fragments arranged by size:
1022 979 823 730 459 248 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 157 189 550 582 713 745 2155 2185 2187
Size: 32 361 32 131 32 1410 30 2
Cuts at: 2187 2217 157
Size: 30 2253
Fragments arranged by size:
2253 1410 361 131 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 960 1352 960
Size: 392 3921
KasI G'GCGC_C
Cuts at: 190 1013 1743 1991 190
Size: 823 730 248 2512
Fragments arranged by size:
2512 823 730 248
KpnI G_GTAC'C
Cuts at: 903 1333 903
Size: 430 3883
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 725 830 873 2984 3168 725
Size: 105 43 2111 184 1870
Fragments arranged by size:
2111 1870 184 105 43
MscI TGG'CCA
Cuts at: 403 778 1057 2074 403
Size: 375 279 1017 2642
Fragments arranged by size:
2642 1017 375 279
MslI CAynn'nnrTG
Cuts at: 544 686 709 1303 2302 2429 544
Size: 142 23 594 999 127 2428
Fragments arranged by size:
2428 999 594 142 127 23
MspA1I CmG'CkG
Cuts at: 1025 1171 1424 1738 2098 3111 3356 1025
Size: 146 253 314 360 1013 245 1982
Fragments arranged by size:
1982 1013 360 314 253 245 146
NaeI GCC'GGC
Cuts at: 137 434 949 1045 1248 2494 137
Size: 297 515 96 203 1246 1956
Fragments arranged by size:
1956 1246 515 297 203 96
NarI GG'CG_CC
Cuts at: 191 1014 1744 1992 191
Size: 823 730 248 2512
Fragments arranged by size:
2512 823 730 248
NcoI C'CATG_G
Cuts at: 773 1052 773
Size: 279 4034
NgoMIV G'CCGG_C
Cuts at: 135 432 947 1043 1246 2492 135
Size: 297 515 96 203 1246 1956
Fragments arranged by size:
1956 1246 515 297 203 96
NspI r_CATG'y
Cuts at: 1366 1866 2773 1366
Size: 500 907 2906
Fragments arranged by size:
2906 907 500
PciI A'CATG_T
Cuts at: 2769 2769
Size: 4313
PfoI T'CCnGG_A
Cuts at: 1070 1133 1070
Size: 63 4250
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 468 468
Size: 4313
PspOMI G'GGCC_C
Cuts at: 168 168
Size: 4313
PstI C_TGCA'G
Cuts at: 221 467 1360 2045 221
Size: 246 893 685 2489
Fragments arranged by size:
2489 893 685 246
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 1738 2098 1738
Size: 360 3953
RsrII CG'GwC_CG
Cuts at: 1220 2508 1220
Size: 1288 3025
SacI G_AGCT'C
Cuts at: 1327 1327
Size: 4313
SalI G'TCGA_C
Cuts at: 958 1350 958
Size: 392 3921
SapI GCTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SexAI A'CCwGG_T
Cuts at: 206 575 206
Size: 369 3944
SfcI C'TryA_G
Cuts at: 33 217 463 519 1356 2041 3034 3225 33
Size: 184 246 56 837 685 993 191 1121
Fragments arranged by size:
1121 993 837 685 246 191 184 56
SfoI GGC'GCC
Cuts at: 192 1015 1745 1993 192
Size: 823 730 248 2512
Fragments arranged by size:
2512 823 730 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SmaI CCC'GGG
Cuts at: 1011 1335 1011
Size: 324 3989
SmlI C'TyrA_G
Cuts at: 421 1468 2383 2875 3137 421
Size: 1047 915 492 262 1597
Fragments arranged by size:
1597 1047 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
StyI C'CwwG_G
Cuts at: 293 773 1052 1447 1801 293
Size: 480 279 395 354 2805
Fragments arranged by size:
2805 480 395 354 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 298 1305 1896 2029 2488 2697 298
Size: 1007 591 133 459 209 1914
Fragments arranged by size:
1914 1007 591 459 209 133
TatI w'GTAC_w
Cuts at: 3828 3828
Size: 4313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4281
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 718 1344 718
Size: 626 3687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 786 1808 786
Size: 1022 3291
XmaI C'CCGG_G
Cuts at: 1009 1333 1009
Size: 324 3989
XmnI GAAnn'nnTTC
Cuts at: 275 275
Size: 4313
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoRV
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI