Restriction map for
pF1KB6559
(Circular) (Six-base) MAPSORT of: pF1KB6559.seq Check: 7296 from: 1 to: 4232
pF1KB6559 4232 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 754 754
Size: 4232
AccI GT'mk_AC
Cuts at: 1270 1270
Size: 4232
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 548 2057 2489 3236 3504 479
Size: 69 1509 432 747 268 1207
Fragments arranged by size:
1509 1207 747 432 268 69
AflIII A'CryG_T
Cuts at: 910 1584 2688 3665 910
Size: 674 1104 977 1477
Fragments arranged by size:
1477 1104 977 674
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AhdI GACnn_n'nnGTC
Cuts at: 565 565
Size: 4232
AleI CACnn'nnGTG
Cuts at: 913 913
Size: 4232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 737 737
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 293 625 1022 1537 3104 293
Size: 332 397 515 1567 1421
Fragments arranged by size:
1567 1421 515 397 332
ApaI G_GGCC'C
Cuts at: 879 879
Size: 4232
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 1000 1236 3585 3841 1000
Size: 236 2349 256 1391
Fragments arranged by size:
2349 1391 256 236
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 262 683 1043 1252 262
Size: 421 360 209 3242
Fragments arranged by size:
3242 421 360 209
BamHI G'GATC_C
Cuts at: 1124 1257 1124
Size: 133 4099
BanI G'GyrC_C
Cuts at: 159 1248 1662 1910 1945 159
Size: 1089 414 248 35 2446
Fragments arranged by size:
2446 1089 414 248 35
BanII G_rGCy'C
Cuts at: 879 1145 1246 2276 879
Size: 266 101 1030 2835
Fragments arranged by size:
2835 1030 266 101
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BbsI GAAGACnn'nnnn_
Cuts at: 262 262
Size: 4232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 782 816 782
Size: 34 4198
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BfrBI ATG'CAT
Cuts at: 1076 1076
Size: 4232
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 879 1857 1950 3006 879
Size: 978 93 1056 2105
Fragments arranged by size:
2105 1056 978 93
BmgBI CAC'GTC
Cuts at: 396 396
Size: 4232
BmrI ACTGGGnnnn_n'
Cuts at: 720 1444 1525 1860 4004 720
Size: 724 81 335 2144 948
Fragments arranged by size:
2144 948 724 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 4232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 847 425
Size: 422 3810
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 2287 2779 3077 1408
Size: 879 492 298 2563
Fragments arranged by size:
2563 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3936 3936
Size: 4232
BsaAI yAC'GTr
Cuts at: 737 911 1585 737
Size: 174 674 3384
Fragments arranged by size:
3384 674 174
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 1663 1911 2613 4109 1663
Size: 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 362 406 478 362
Size: 44 72 4116
Fragments arranged by size:
4116 72 44
BseYI C'CCAG_C
Cuts at: 286 549 763 1657 2415 2992 3488 286
Size: 263 214 894 758 577 496 1030
Fragments arranged by size:
1030 894 758 577 496 263 214
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 499 317
Size: 182 4050
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1246 2024 2214 3006 1246
Size: 778 190 792 2472
Fragments arranged by size:
2472 792 778 190
BsmI GAATG_Cn'
Cuts at: 1078 1078
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 879 1145 1246 1857 1950 2024 2214 2276 3006
Size: 266 101 611 93 74 190 62 730
Cuts at: 3006 879
Size: 2105
Fragments arranged by size:
2105 730 611 266 190 101 93 74
62
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 754 928 1282 1496 1798 2179 754
Size: 174 354 214 302 381 2807
Fragments arranged by size:
2807 381 354 302 214 174
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4062 4227 50
Size: 2474 54 1484 165 55
Fragments arranged by size:
2474 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 494 2144 494
Size: 1650 2582
BsrFI r'CCGG_y
Cuts at: 327 2230 2411 2620 327
Size: 1903 181 209 1939
Fragments arranged by size:
1939 1903 209 181
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 1193 1609 1193
Size: 416 3816
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 763 787 763
Size: 24 4208
BstYI r'GATC_y
Cuts at: 1 1124 1257 1724 1747 2082 2328 3340 3426
Size: 1123 133 467 23 335 246 1012 86
Cuts at: 3426 3438 3784 1
Size: 12 346 449
Fragments arranged by size:
1123 1012 467 449 346 335 246 133
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 583 583
Size: 4232
BtgI C'CryG_G
Cuts at: 107 182 590 1145 107
Size: 75 408 555 3194
Fragments arranged by size:
3194 555 408 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 188 2182 2363 2526 188
Size: 1994 181 163 1894
Fragments arranged by size:
1994 1894 181 163
BtsI GCAGTG_nn'
Cuts at: 1475 1475
Size: 4232
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 179 225 1817 1991 2382 2409 3754 179
Size: 46 1592 174 391 27 1345 657
Fragments arranged by size:
1592 1345 657 391 174 46 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 121 352 455 593 703 1186 2255 2465 121
Size: 231 103 138 110 483 1069 210 1888
Fragments arranged by size:
1888 1069 483 231 210 138 110 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 202 2440 2750 2896 4042 202
Size: 2238 310 146 1146 392
Fragments arranged by size:
2238 1146 392 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1244
Size: 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 479 548 847 2057 2489 3236 3504 425
Size: 54 69 299 1210 432 747 268 1153
Fragments arranged by size:
1210 1153 747 432 299 268 69 54
EcoNI CCTnn'n_nnAGG
Cuts at: 883 883
Size: 4232
EcoO109I rG'GnC_Cy
Cuts at: 485 546 559 875 876 1371 485
Size: 61 13 316 1 495 3346
Fragments arranged by size:
3346 495 316 61 13 1
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 1666 1914 2936 3915 1666
Size: 248 1022 979 1983
Fragments arranged by size:
1983 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 551 583 2074 2104 2106 2136 551
Size: 32 1491 30 2 30 2647
Fragments arranged by size:
2647 1491 32 30 30 2
HincII GTy'rAC
Cuts at: 1271 1271
Size: 4232
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3087 2903
Size: 184 4048
MscI TGG'CCA
Cuts at: 1993 1993
Size: 4232
MslI CAynn'nnrTG
Cuts at: 913 2221 2348 913
Size: 1308 127 2797
Fragments arranged by size:
2797 1308 127
MspA1I CmG'CkG
Cuts at: 513 553 759 1343 1657 2017 3030 3275 513
Size: 40 206 584 314 360 1013 245 1470
Fragments arranged by size:
1470 1013 584 360 314 245 206 40
NaeI GCC'GGC
Cuts at: 329 2413 329
Size: 2084 2148
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NcoI C'CATG_G
Cuts at: 107 590 107
Size: 483 3749
NgoMIV G'CCGG_C
Cuts at: 327 2411 327
Size: 2084 2148
NsiI A_TGCA'T
Cuts at: 1078 1078
Size: 4232
NspI r_CATG'y
Cuts at: 1285 1785 2692 1285
Size: 500 907 2825
Fragments arranged by size:
2825 907 500
PasI CC'CwG_GG
Cuts at: 233 879 954 233
Size: 646 75 3511
Fragments arranged by size:
3511 646 75
PciI A'CATG_T
Cuts at: 2688 2688
Size: 4232
PflMI CCAn_nnn'nTGG
Cuts at: 293 293
Size: 4232
PfoI T'CCnGG_A
Cuts at: 380 857 380
Size: 477 3755
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PmlI CAC'GTG
Cuts at: 737 911 737
Size: 174 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PpuMI rG'GwC_Cy
Cuts at: 559 559
Size: 4232
PspOMI G'GGCC_C
Cuts at: 875 875
Size: 4232
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 958 990 958
Size: 32 4200
PstI C_TGCA'G
Cuts at: 318 1018 1123 1279 1964 318
Size: 700 105 156 685 2586
Fragments arranged by size:
2586 700 685 156 105
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 513 759 1657 2017 513
Size: 246 898 360 2728
Fragments arranged by size:
2728 898 360 246
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 1246 1246
Size: 4232
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 352 593 2255 2465 352
Size: 241 1662 210 2119
Fragments arranged by size:
2119 1662 241 210
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SfcI C'TryA_G
Cuts at: 33 314 993 1014 1104 1119 1275 1960 2953
Size: 281 679 21 90 15 156 685 993
Cuts at: 2953 3144 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 679 281 191 156 90
21 15
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 1254 1254
Size: 4232
SmlI C'TyrA_G
Cuts at: 1387 2302 2794 3056 1387
Size: 915 492 262 2563
Fragments arranged by size:
2563 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
StuI AGG'CCT
Cuts at: 889 889
Size: 4232
StyI C'CwwG_G
Cuts at: 107 590 1152 1366 1720 107
Size: 483 562 214 354 2619
Fragments arranged by size:
2619 562 483 354 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1815 1948 2407 2616 1815
Size: 133 459 209 3431
Fragments arranged by size:
3431 459 209 133
TatI w'GTAC_w
Cuts at: 974 3747 974
Size: 2773 1459
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4200
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1727 1727
Size: 4232
XmaI C'CCGG_G
Cuts at: 1252 1252
Size: 4232
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BbvCI
BglI BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI