Restriction map for
pF1KB6557
(Circular) (Six-base) MAPSORT of: pF1KB6557.seq Check: 4408 from: 1 to: 4229
pF1KB6557 4229 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 404 404
Size: 4229
AccI GT'mk_AC
Cuts at: 167 1267 167
Size: 1100 3129
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2486 3233 3501 2054
Size: 432 747 268 2782
Fragments arranged by size:
2782 747 432 268
AflIII A'CryG_T
Cuts at: 1581 2685 3662 1581
Size: 1104 977 2148
Fragments arranged by size:
2148 1104 977
AgeI A'CCGG_T
Cuts at: 2617 2617
Size: 4229
AhdI GACnn_n'nnGTC
Cuts at: 813 813
Size: 4229
AleI CACnn'nnGTG
Cuts at: 709 709
Size: 4229
AlwNI CAG_nnn'CTG
Cuts at: 1534 3101 1534
Size: 1567 2662
ApaLI G'TGCA_C
Cuts at: 2999 2999
Size: 4229
ApoI r'AATT_y
Cuts at: 1233 3582 3838 1233
Size: 2349 256 1624
Fragments arranged by size:
2349 1624 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 1249 1249
Size: 4229
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4229
BanI G'GyrC_C
Cuts at: 195 392 1086 1149 1245 1659 1907 1942 195
Size: 197 694 63 96 414 248 35 2482
Fragments arranged by size:
2482 694 414 248 197 96 63 35
BanII G_rGCy'C
Cuts at: 226 1243 2273 226
Size: 1017 1030 2182
Fragments arranged by size:
2182 1030 1017
BbeI G_GCGC'C
Cuts at: 1663 1911 1663
Size: 248 3981
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2888 4229 2127
Size: 761 1341 2127
Fragments arranged by size:
2127 1341 761
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4229
BglI GCCn_nnn'nGGC
Cuts at: 359 359
Size: 4229
BglII A'GATC_T
Cuts at: 1106 1744 3781 1106
Size: 638 2037 1554
Fragments arranged by size:
2037 1554 638
BlpI GC'TnA_GC
Cuts at: 448 1341 448
Size: 893 3336
Bme1580I G_kGCm'C
Cuts at: 755 1854 1947 3003 755
Size: 1099 93 1056 1981
Fragments arranged by size:
1981 1099 1056 93
BmgBI CAC'GTC
Cuts at: 231 275 782 231
Size: 44 507 3678
Fragments arranged by size:
3678 507 44
BmrI ACTGGGnnnn_n'
Cuts at: 267 358 1441 1522 1857 4001 267
Size: 91 1083 81 335 2144 495
Fragments arranged by size:
2144 1083 495 335 91 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 793 1005 710
Size: 83 212 3934
Fragments arranged by size:
3934 212 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 811 1405 2284 2776 3074 571
Size: 240 594 879 492 298 1726
Fragments arranged by size:
1726 879 594 492 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 648 1051 3933 648
Size: 403 2882 944
Fragments arranged by size:
2882 944 403
BsaAI yAC'GTr
Cuts at: 1582 1582
Size: 4229
BsaBI GATnn'nnATC
Cuts at: 6 1743 3780 6
Size: 1737 2037 455
Fragments arranged by size:
2037 1737 455
BsaHI Gr'CG_yC
Cuts at: 1660 1908 2610 4106 1660
Size: 248 702 1496 1783
Fragments arranged by size:
1783 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1418 1939 2617 2891 3038 3449 1418
Size: 521 678 274 147 411 2198
Fragments arranged by size:
2198 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 370 400 607 628 637 658 766 796 370
Size: 30 207 21 9 21 108 30 3803
Fragments arranged by size:
3803 207 108 30 30 21 21 9
BseYI C'CCAG_C
Cuts at: 579 754 1125 1654 2412 2989 3485 579
Size: 175 371 529 758 577 496 1323
Fragments arranged by size:
1323 758 577 529 496 371 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 226
Size: 4229
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3025 104
Size: 1667 46 1208 1308
Fragments arranged by size:
1667 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 335 449 1243 2021 2211 3003 335
Size: 114 794 778 190 792 1561
Fragments arranged by size:
1561 794 792 778 190 114
Bsp1286I G_dGCh'C
Cuts at: 226 335 449 755 1243 1854 1947 2021 2211
Size: 109 114 306 488 611 93 74 190
Cuts at: 2211 2273 3003 226
Size: 62 730 1452
Fragments arranged by size:
1452 730 611 488 306 190 114 109
93 74 62
BspEI T'CCGG_A
Cuts at: 3449 3449
Size: 4229
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 317 404 1279 1493 1795 2176 317
Size: 87 875 214 302 381 2370
Fragments arranged by size:
2370 875 381 302 214 87
BsrBI CCG'CTC
Cuts at: 50 458 2521 2575 4059 4224 50
Size: 408 2063 54 1484 165 55
Fragments arranged by size:
2063 1484 408 165 55 54
BsrDI GCAATG_nn'
Cuts at: 666 2141 666
Size: 1475 2754
BsrFI r'CCGG_y
Cuts at: 767 2227 2408 2617 767
Size: 1460 181 209 2379
Fragments arranged by size:
2379 1460 209 181
BssSI C'ACGA_G
Cuts at: 2500 2858 2500
Size: 358 3871
BstAPI GCAn_nnn'nTGC
Cuts at: 1606 1606
Size: 4229
BstBI TT'CG_AA
Cuts at: 2590 2590
Size: 4229
BstXI CCAn_nnnn'nTGG
Cuts at: 561 1083 1179 561
Size: 522 96 3611
Fragments arranged by size:
3611 522 96
BstYI r'GATC_y
Cuts at: 1 1106 1254 1721 1744 2079 2325 3337 3423
Size: 1105 148 467 23 335 246 1012 86
Cuts at: 3423 3435 3781 1
Size: 12 346 449
Fragments arranged by size:
1105 1012 467 449 346 335 246 148
86 23 12
BtgI C'CryG_G
Cuts at: 107 124 336 433 1037 107
Size: 17 212 97 604 3299
Fragments arranged by size:
3299 604 212 97 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2179 2360 2523 2179
Size: 181 163 3885
Fragments arranged by size:
3885 181 163
BtsI GCAGTG_nn'
Cuts at: 235 1202 1472 235
Size: 967 270 2992
Fragments arranged by size:
2992 967 270
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 1935 2793 1935
Size: 858 3371
EaeI y'GGCC_r
Cuts at: 745 1814 1988 2379 2406 3751 745
Size: 1069 174 391 27 1345 1223
Fragments arranged by size:
1345 1223 1069 391 174 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4229
EarI CTCTTCn'nnn_
Cuts at: 2252 2462 2252
Size: 210 4019
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2437 2747 2893 4039 2437
Size: 310 146 1146 2627
Fragments arranged by size:
2627 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1241 1241
Size: 4229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 793 1005 2054 2486 3233 3501 710
Size: 83 212 1049 432 747 268 1438
Fragments arranged by size:
1438 1049 747 432 268 212 83
EcoO109I rG'GnC_Cy
Cuts at: 576 1141 1368 576
Size: 565 227 3437
Fragments arranged by size:
3437 565 227
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 4229
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4229
HaeII r_GCGC'y
Cuts at: 219 1663 1911 2933 3912 219
Size: 1444 248 1022 979 536
Fragments arranged by size:
1444 1022 979 536 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 168 200 799 831 839 871 2071 2101 2103
Size: 32 599 32 8 32 1200 30 2
Cuts at: 2103 2133 168
Size: 30 2264
Fragments arranged by size:
2264 1200 599 32 32 32 30 30
8 2
HincII GTy'rAC
Cuts at: 1268 1268
Size: 4229
KasI G'GCGC_C
Cuts at: 1659 1907 1659
Size: 248 3981
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 485 911 2900 3084 485
Size: 426 1989 184 1630
Fragments arranged by size:
1989 1630 426 184
MscI TGG'CCA
Cuts at: 1990 1990
Size: 4229
MslI CAynn'nnrTG
Cuts at: 240 709 2218 2345 240
Size: 469 1509 127 2124
Fragments arranged by size:
2124 1509 469 127
MspA1I CmG'CkG
Cuts at: 327 435 452 491 596 1133 1195 1340 1654
Size: 108 17 39 105 537 62 145 314
Cuts at: 1654 2014 3027 3272 327
Size: 360 1013 245 1284
Fragments arranged by size:
1284 1013 537 360 314 245 145 108
105 62 39 17
NaeI GCC'GGC
Cuts at: 769 2410 769
Size: 1641 2588
NarI GG'CG_CC
Cuts at: 1660 1908 1660
Size: 248 3981
NcoI C'CATG_G
Cuts at: 107 336 107
Size: 229 4000
NgoMIV G'CCGG_C
Cuts at: 767 2408 767
Size: 1641 2588
NspI r_CATG'y
Cuts at: 421 615 877 1282 1782 2689 421
Size: 194 262 405 500 907 1961
Fragments arranged by size:
1961 907 500 405 262 194
PasI CC'CwG_GG
Cuts at: 666 1117 666
Size: 451 3778
PciI A'CATG_T
Cuts at: 2685 2685
Size: 4229
PflMI CCAn_nnn'nTGG
Cuts at: 342 342
Size: 4229
PfoI T'CCnGG_A
Cuts at: 857 857
Size: 4229
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 4197
PpuMI rG'GwC_Cy
Cuts at: 576 1141 576
Size: 565 3664
PstI C_TGCA'G
Cuts at: 490 1276 1961 490
Size: 786 685 2758
Fragments arranged by size:
2758 786 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 327 452 491 1654 2014 327
Size: 125 39 1163 360 2542
Fragments arranged by size:
2542 1163 360 125 39
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 1243 1243
Size: 4229
SacII CC_GC'GG
Cuts at: 436 436
Size: 4229
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SanDI GG'GwC_CC
Cuts at: 1141 1141
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 2252 2462 2252
Size: 210 4019
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 3746 3746
Size: 4229
SfcI C'TryA_G
Cuts at: 33 486 1272 1957 2950 3141 33
Size: 453 786 685 993 191 1121
Fragments arranged by size:
1121 993 786 685 453 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 359 359
Size: 4229
SfoI GGC'GCC
Cuts at: 1661 1909 1661
Size: 248 3981
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4229
SmlI C'TyrA_G
Cuts at: 586 826 1384 2299 2791 3053 586
Size: 240 558 915 492 262 1762
Fragments arranged by size:
1762 915 558 492 262 240
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3729
StyI C'CwwG_G
Cuts at: 107 336 473 953 1363 1717 107
Size: 229 137 480 410 354 2619
Fragments arranged by size:
2619 480 410 354 229 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1812 1945 2404 2613 1812
Size: 133 459 209 3428
Fragments arranged by size:
3428 459 209 133
TatI w'GTAC_w
Cuts at: 635 932 3744 635
Size: 297 2812 1120
Fragments arranged by size:
2812 1120 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 370 402 501 533 607 639 766 798 963
Size: 32 99 32 74 32 127 32 165
Cuts at: 963 995 3746 3778 370
Size: 32 2751 32 821
Fragments arranged by size:
2751 821 165 127 99 74 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 182 2026 182
Size: 1844 2385
XbaI T'CTAG_A
Cuts at: 1260 1260
Size: 4229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1080 1724 1080
Size: 644 3585
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4229
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI