Restriction map for
pF1KB6548
(Circular) (Six-base) MAPSORT of: pF1KB6548.seq Check: 8580 from: 1 to: 4289
pF1KB6548 4289 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1327 1327
Size: 4289
Acc65I G'GTAC_C
Cuts at: 514 1305 514
Size: 791 3498
AclI AA'CG_TT
Cuts at: 4129 4129
Size: 4289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 685 916 1187 2114 2546 3293 3561 130
Size: 555 231 271 927 432 747 268 858
Fragments arranged by size:
927 858 747 555 432 271 268 231
AflII C'TTAA_G
Cuts at: 1090 1090
Size: 4289
AflIII A'CryG_T
Cuts at: 219 875 1641 2745 3722 219
Size: 656 766 1104 977 786
Fragments arranged by size:
1104 977 786 766 656
AgeI A'CCGG_T
Cuts at: 2677 2677
Size: 4289
AlwNI CAG_nnn'CTG
Cuts at: 237 348 532 663 1053 1594 3161 237
Size: 111 184 131 390 541 1567 1365
Fragments arranged by size:
1567 1365 541 390 184 131 111
ApaI G_GGCC'C
Cuts at: 983 1162 983
Size: 179 4110
ApaLI G'TGCA_C
Cuts at: 3059 3059
Size: 4289
ApoI r'AATT_y
Cuts at: 1293 3642 3898 1293
Size: 2349 256 1684
Fragments arranged by size:
2349 1684 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4289
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
AvaI C'yCGr_G
Cuts at: 283 837 1309 283
Size: 554 472 3263
Fragments arranged by size:
3263 554 472
BamHI G'GATC_C
Cuts at: 1314 1314
Size: 4289
BanI G'GyrC_C
Cuts at: 514 1305 1719 1967 2002 514
Size: 791 414 248 35 2801
Fragments arranged by size:
2801 791 414 248 35
BanII G_rGCy'C
Cuts at: 542 830 983 1073 1162 1303 2333 542
Size: 288 153 90 89 141 1030 2498
Fragments arranged by size:
2498 1030 288 153 141 90 89
BbeI G_GCGC'C
Cuts at: 1723 1971 1723
Size: 248 4041
BbvCI CC'TCA_GC
Cuts at: 680 994 680
Size: 314 3975
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4255
BciVI GTATCCnnnnn_n'
Cuts at: 2187 2948 4289 2187
Size: 761 1341 2187
Fragments arranged by size:
2187 1341 761
BclI T'GATC_A
Cuts at: 1809 1809
Size: 4289
BglI GCCn_nnn'nGGC
Cuts at: 481 668 481
Size: 187 4102
BglII A'GATC_T
Cuts at: 1804 3841 1804
Size: 2037 2252
BlpI GC'TnA_GC
Cuts at: 1067 1115 1401 1067
Size: 48 286 3955
Fragments arranged by size:
3955 286 48
Bme1580I G_kGCm'C
Cuts at: 182 983 1162 1914 2007 3063 182
Size: 801 179 752 93 1056 1408
Fragments arranged by size:
1408 1056 801 752 179 93
BmgBI CAC'GTC
Cuts at: 876 876
Size: 4289
BmrI ACTGGGnnnn_n'
Cuts at: 1501 1582 1917 4061 1501
Size: 81 335 2144 1729
Fragments arranged by size:
2144 1729 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 4289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 120
Size: 4289
Bpu10I CC'TnA_GC
Cuts at: 680 994 680
Size: 314 3975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 2344 2836 3134 1465
Size: 879 492 298 2620
Fragments arranged by size:
2620 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 312 3993 312
Size: 3681 608
BsaAI yAC'GTr
Cuts at: 222 1642 222
Size: 1420 2869
BsaBI GATnn'nnATC
Cuts at: 6 1225 1803 3840 6
Size: 1219 578 2037 455
Fragments arranged by size:
2037 1219 578 455
BsaHI Gr'CG_yC
Cuts at: 1007 1720 1968 2670 4166 1007
Size: 713 248 702 1496 1130
Fragments arranged by size:
1496 1130 713 702 248
BsaWI w'CCGG_w
Cuts at: 1478 1999 2677 2951 3098 3509 1478
Size: 521 678 274 147 411 2258
Fragments arranged by size:
2258 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 862 892 862
Size: 30 4259
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 346 444 634 709 712 715 820 346
Size: 98 190 75 3 3 105 3815
Fragments arranged by size:
3815 190 105 98 75 3 3
BseYI C'CCAG_C
Cuts at: 182 988 1085 1177 1273 1714 2472 3049 3545
Size: 806 97 92 96 441 758 577 496
Cuts at: 3545 182
Size: 926
Fragments arranged by size:
926 806 758 577 496 441 97 96
92
BsiEI CG_ry'CG
Cuts at: 104 962 1831 1877 3085 104
Size: 858 869 46 1208 1308
Fragments arranged by size:
1308 1208 869 858 46
BsiHKAI G_wGCw'C
Cuts at: 128 1303 2081 2271 3063 128
Size: 1175 778 190 792 1354
Fragments arranged by size:
1354 1175 792 778 190
BsmI GAATG_Cn'
Cuts at: 156 156
Size: 4289
Bsp1286I G_dGCh'C
Cuts at: 128 182 542 830 983 1073 1162 1303 1914
Size: 54 360 288 153 90 89 141 611
Cuts at: 1914 2007 2081 2271 2333 3063 128
Size: 93 74 190 62 730 1354
Fragments arranged by size:
1354 730 611 360 288 190 153 141
93 90 89 74 62 54
BspEI T'CCGG_A
Cuts at: 3509 3509
Size: 4289
BspHI T'CATG_A
Cuts at: 3465 3465
Size: 4289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1339 1553 1855 2236 1339
Size: 214 302 381 3392
Fragments arranged by size:
3392 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2581 2635 4119 4284 50
Size: 2531 54 1484 165 55
Fragments arranged by size:
2531 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2201 2201
Size: 4289
BsrFI r'CCGG_y
Cuts at: 957 1119 2287 2468 2677 957
Size: 162 1168 181 209 2569
Fragments arranged by size:
2569 1168 209 181 162
BssSI C'ACGA_G
Cuts at: 2560 2918 2560
Size: 358 3931
BstAPI GCAn_nnn'nTGC
Cuts at: 1666 1666
Size: 4289
BstBI TT'CG_AA
Cuts at: 2650 2650
Size: 4289
BstEII G'GTnAC_C
Cuts at: 243 243
Size: 4289
BstXI CCAn_nnnn'nTGG
Cuts at: 936 936
Size: 4289
BstYI r'GATC_y
Cuts at: 1 233 449 520 1202 1314 1781 1804 2139
Size: 232 216 71 682 112 467 23 335
Cuts at: 2139 2385 3397 3483 3495 3841 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 682 467 449 346 335 246 232
216 112 86 71 23 12
BtgI C'CryG_G
Cuts at: 884 1211 884
Size: 327 3962
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2239 2420 2583 2239
Size: 181 163 3945
Fragments arranged by size:
3945 181 163
BtsI GCAGTG_nn'
Cuts at: 1532 1532
Size: 4289
DraI TTT'AAA
Cuts at: 1288 1288
Size: 4289
DrdI GACnn_nn'nnGTC
Cuts at: 1995 2853 1995
Size: 858 3431
EaeI y'GGCC_r
Cuts at: 269 761 959 1874 2048 2439 2466 3811 269
Size: 492 198 915 174 391 27 1345 747
Fragments arranged by size:
1345 915 747 492 391 198 174 27
EagI C'GGCC_G
Cuts at: 959 1874 959
Size: 915 3374
EarI CTCTTCn'nnn_
Cuts at: 593 635 1028 1285 2312 2522 593
Size: 42 393 257 1027 210 2360
Fragments arranged by size:
2360 1027 393 257 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 638 2497 2807 2953 4099 638
Size: 1859 310 146 1146 828
Fragments arranged by size:
1859 1146 828 310 146
EcoICRI GAG'CTC
Cuts at: 1301 1301
Size: 4289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 130 685 916 1187 2114 2546 3293 3561
Size: 10 555 231 271 927 432 747 268
Cuts at: 3561 120
Size: 848
Fragments arranged by size:
927 848 747 555 432 271 268 231
10
EcoO109I rG'GnC_Cy
Cuts at: 396 861 979 1428 396
Size: 465 118 449 3257
Fragments arranged by size:
3257 465 449 118
EcoRI G'AATT_C
Cuts at: 1293 1293
Size: 4289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 4289
FspI TGC'GCA
Cuts at: 2070 2070
Size: 4289
HaeII r_GCGC'y
Cuts at: 709 1723 1971 2993 3972 709
Size: 1014 248 1022 979 1026
Fragments arranged by size:
1026 1022 1014 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 862 865 894 897 2131 2161 2163 2193 862
Size: 3 29 3 1234 30 2 30 2958
Fragments arranged by size:
2958 1234 30 30 29 3 3 2
HincII GTy'rAC
Cuts at: 1328 1328
Size: 4289
KasI G'GCGC_C
Cuts at: 1719 1967 1719
Size: 248 4041
KpnI G_GTAC'C
Cuts at: 518 1309 518
Size: 791 3498
MluI A'CGCG_T
Cuts at: 3722 3722
Size: 4289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2960 3144 2960
Size: 184 4105
MscI TGG'CCA
Cuts at: 2050 2050
Size: 4289
MslI CAynn'nnrTG
Cuts at: 2278 2405 2278
Size: 127 4162
MspA1I CmG'CkG
Cuts at: 1141 1400 1714 2074 3087 3332 1141
Size: 259 314 360 1013 245 2098
Fragments arranged by size:
2098 1013 360 314 259 245
NaeI GCC'GGC
Cuts at: 959 2470 959
Size: 1511 2778
NarI GG'CG_CC
Cuts at: 1720 1968 1720
Size: 248 4041
NcoI C'CATG_G
Cuts at: 884 1211 884
Size: 327 3962
NgoMIV G'CCGG_C
Cuts at: 957 2468 957
Size: 1511 2778
NspI r_CATG'y
Cuts at: 1342 1842 2749 1342
Size: 500 907 2882
Fragments arranged by size:
2882 907 500
PasI CC'CwG_GG
Cuts at: 526 936 526
Size: 410 3879
PciI A'CATG_T
Cuts at: 2745 2745
Size: 4289
PflMI CCAn_nnn'nTGG
Cuts at: 237 303 1212 237
Size: 66 909 3314
Fragments arranged by size:
3314 909 66
PfoI T'CCnGG_A
Cuts at: 422 563 1241 422
Size: 141 678 3470
Fragments arranged by size:
3470 678 141
PmeI GTTT'AAAC
Cuts at: 1288 1288
Size: 4289
PmlI CAC'GTG
Cuts at: 222 222
Size: 4289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 4257
PpuMI rG'GwC_Cy
Cuts at: 396 861 396
Size: 465 3824
PspOMI G'GGCC_C
Cuts at: 979 1158 979
Size: 179 4110
PstI C_TGCA'G
Cuts at: 537 1336 2021 537
Size: 799 685 2805
Fragments arranged by size:
2805 799 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4289
PvuII CAG'CTG
Cuts at: 1141 1714 2074 1141
Size: 573 360 3356
Fragments arranged by size:
3356 573 360
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 4289
SacI G_AGCT'C
Cuts at: 1303 1303
Size: 4289
SalI G'TCGA_C
Cuts at: 1326 1326
Size: 4289
SapI GCTCTTCn'nnn_
Cuts at: 2312 2522 2312
Size: 210 4079
SbfI CC_TGCA'GG
Cuts at: 1336 1336
Size: 4289
ScaI AGT'ACT
Cuts at: 3806 3806
Size: 4289
SfcI C'TryA_G
Cuts at: 33 533 1332 2017 3010 3201 33
Size: 500 799 685 993 191 1121
Fragments arranged by size:
1121 993 799 685 500 191
SfoI GGC'GCC
Cuts at: 1721 1969 1721
Size: 248 4041
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
SmaI CCC'GGG
Cuts at: 839 1311 839
Size: 472 3817
SmlI C'TyrA_G
Cuts at: 1090 1444 2359 2851 3113 1090
Size: 354 915 492 262 2266
Fragments arranged by size:
2266 915 492 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4289
SphI G_CATG'C
Cuts at: 1342 1842 1342
Size: 500 3789
SrfI GCCC'GGGC
Cuts at: 839 839
Size: 4289
StyI C'CwwG_G
Cuts at: 467 689 884 1013 1211 1423 1777 467
Size: 222 195 129 198 212 354 2979
Fragments arranged by size:
2979 354 222 212 198 195 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 928 1002 1872 2005 2464 2673 928
Size: 74 870 133 459 209 2544
Fragments arranged by size:
2544 870 459 209 133 74
TatI w'GTAC_w
Cuts at: 317 1235 3804 317
Size: 918 2569 802
Fragments arranged by size:
2569 918 802
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3806 3838 3806
Size: 32 4257
Tth111I GACn'n_nGTC
Cuts at: 2086 2086
Size: 4289
XbaI T'CTAG_A
Cuts at: 1320 1320
Size: 4289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1104 1218 1784 1104
Size: 114 566 3609
Fragments arranged by size:
3609 566 114
XmaI C'CCGG_G
Cuts at: 837 1309 837
Size: 472 3817
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmtI BsgI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI