Restriction map for
pF1KB6546
(Circular) (Six-base) MAPSORT of: pF1KB6546.seq Check: 6010 from: 1 to: 4289
pF1KB6546 4289 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 534 534
Size: 4289
AccI GT'mk_AC
Cuts at: 1327 1327
Size: 4289
Acc65I G'GTAC_C
Cuts at: 1305 1305
Size: 4289
AclI AA'CG_TT
Cuts at: 4129 4129
Size: 4289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 739 805 2114 2546 3293 3561 628
Size: 111 66 1309 432 747 268 1356
Fragments arranged by size:
1356 1309 747 432 268 111 66
AflII C'TTAA_G
Cuts at: 262 262
Size: 4289
AflIII A'CryG_T
Cuts at: 1641 2745 3722 1641
Size: 1104 977 2208
Fragments arranged by size:
2208 1104 977
AgeI A'CCGG_T
Cuts at: 2677 2677
Size: 4289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 940 940
Size: 4289
AlwNI CAG_nnn'CTG
Cuts at: 1594 3161 1594
Size: 1567 2722
ApaLI G'TGCA_C
Cuts at: 3059 3059
Size: 4289
ApoI r'AATT_y
Cuts at: 555 936 1131 1293 3642 3898 555
Size: 381 195 162 2349 256 946
Fragments arranged by size:
2349 946 381 256 195 162
AseI AT'TA_AT
Cuts at: 20 20
Size: 4289
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
AvaI C'yCGr_G
Cuts at: 1309 1309
Size: 4289
BamHI G'GATC_C
Cuts at: 696 1314 696
Size: 618 3671
BanI G'GyrC_C
Cuts at: 1305 1719 1967 2002 1305
Size: 414 248 35 3592
Fragments arranged by size:
3592 414 248 35
BanII G_rGCy'C
Cuts at: 1303 2333 1303
Size: 1030 3259
BbeI G_GCGC'C
Cuts at: 1723 1971 1723
Size: 248 4041
BbsI GAAGACnn'nnnn_
Cuts at: 251 716 905 1148 251
Size: 465 189 243 3392
Fragments arranged by size:
3392 465 243 189
BbvCI CC'TCA_GC
Cuts at: 520 520
Size: 4289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 386 420 386
Size: 34 4255
BciVI GTATCCnnnnn_n'
Cuts at: 2187 2948 4289 2187
Size: 761 1341 2187
Fragments arranged by size:
2187 1341 761
BclI T'GATC_A
Cuts at: 1809 1809
Size: 4289
BfrBI ATG'CAT
Cuts at: 391 772 391
Size: 381 3908
BglII A'GATC_T
Cuts at: 729 1804 3841 729
Size: 1075 2037 1177
Fragments arranged by size:
2037 1177 1075
BlpI GC'TnA_GC
Cuts at: 1401 1401
Size: 4289
Bme1580I G_kGCm'C
Cuts at: 882 1914 2007 3063 882
Size: 1032 93 1056 2108
Fragments arranged by size:
2108 1056 1032 93
BmrI ACTGGGnnnn_n'
Cuts at: 588 786 1501 1582 1917 4061 588
Size: 198 715 81 335 2144 816
Fragments arranged by size:
2144 816 715 335 198 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 4289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 499 164
Size: 335 3954
Bpu10I CC'TnA_GC
Cuts at: 520 520
Size: 4289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 1465 2344 2836 3134 803
Size: 662 879 492 298 1958
Fragments arranged by size:
1958 879 662 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3993 3993
Size: 4289
BsaAI yAC'GTr
Cuts at: 1642 1642
Size: 4289
BsaBI GATnn'nnATC
Cuts at: 6 701 1803 3840 6
Size: 695 1102 2037 455
Fragments arranged by size:
2037 1102 695 455
BsaHI Gr'CG_yC
Cuts at: 1720 1968 2670 4166 1720
Size: 248 702 1496 1843
Fragments arranged by size:
1843 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1478 1999 2677 2951 3098 3509 1478
Size: 521 678 274 147 411 2258
Fragments arranged by size:
2258 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 162 192 162
Size: 30 4259
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 502 502
Size: 4289
BseYI C'CCAG_C
Cuts at: 1714 2472 3049 3545 1714
Size: 758 577 496 2458
Fragments arranged by size:
2458 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 910
Size: 4289
BsiEI CG_ry'CG
Cuts at: 104 1831 1877 3085 104
Size: 1727 46 1208 1308
Fragments arranged by size:
1727 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1303 2081 2271 3063 1303
Size: 778 190 792 2529
Fragments arranged by size:
2529 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 882 1303 1914 2007 2081 2271 2333 3063 882
Size: 421 611 93 74 190 62 730 2108
Fragments arranged by size:
2108 730 611 421 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3509 3509
Size: 4289
BspHI T'CATG_A
Cuts at: 147 3465 147
Size: 3318 971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 534 1339 1553 1855 2236 534
Size: 805 214 302 381 2587
Fragments arranged by size:
2587 805 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2581 2635 4119 4284 50
Size: 2531 54 1484 165 55
Fragments arranged by size:
2531 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2201 2201
Size: 4289
BsrFI r'CCGG_y
Cuts at: 2287 2468 2677 2287
Size: 181 209 3899
Fragments arranged by size:
3899 209 181
BssSI C'ACGA_G
Cuts at: 2560 2918 2560
Size: 358 3931
BstAPI GCAn_nnn'nTGC
Cuts at: 1666 1666
Size: 4289
BstBI TT'CG_AA
Cuts at: 2650 2650
Size: 4289
BstYI r'GATC_y
Cuts at: 1 611 696 729 1078 1314 1781 1804 2139
Size: 610 85 33 349 236 467 23 335
Cuts at: 2139 2385 3397 3483 3495 3841 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 610 467 449 349 346 335 246
236 86 85 33 23 12
BtgI C'CryG_G
Cuts at: 107 356 615 107
Size: 249 259 3781
Fragments arranged by size:
3781 259 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2239 2420 2583 2239
Size: 181 163 3945
Fragments arranged by size:
3945 181 163
BtsI GCAGTG_nn'
Cuts at: 233 900 1237 1532 233
Size: 667 337 295 2990
Fragments arranged by size:
2990 667 337 295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 338 373 338
Size: 35 4254
DraI TTT'AAA
Cuts at: 560 1288 560
Size: 728 3561
DrdI GACnn_nn'nnGTC
Cuts at: 1995 2853 1995
Size: 858 3431
EaeI y'GGCC_r
Cuts at: 1874 2048 2439 2466 3811 1874
Size: 174 391 27 1345 2352
Fragments arranged by size:
2352 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1874 1874
Size: 4289
EarI CTCTTCn'nnn_
Cuts at: 292 421 715 781 903 2312 2522 292
Size: 129 294 66 122 1409 210 2059
Fragments arranged by size:
2059 1409 294 210 129 122 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2497 2807 2953 4099 2497
Size: 310 146 1146 2687
Fragments arranged by size:
2687 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1301 1301
Size: 4289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 499 628 739 805 2114 2546 3293 3561
Size: 335 129 111 66 1309 432 747 268
Cuts at: 3561 164
Size: 892
Fragments arranged by size:
1309 892 747 432 335 268 129 111
66
EcoNI CCTnn'n_nnAGG
Cuts at: 1016 1016
Size: 4289
EcoO109I rG'GnC_Cy
Cuts at: 1428 1428
Size: 4289
EcoRI G'AATT_C
Cuts at: 1293 1293
Size: 4289
EcoRV GAT'ATC
Cuts at: 132 132
Size: 4289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 4289
FspI TGC'GCA
Cuts at: 2070 2070
Size: 4289
HaeII r_GCGC'y
Cuts at: 1723 1971 2993 3972 1723
Size: 248 1022 979 2040
Fragments arranged by size:
2040 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 714 746 2131 2161 2163 2193 714
Size: 32 1385 30 2 30 2810
Fragments arranged by size:
2810 1385 32 30 30 2
HincII GTy'rAC
Cuts at: 1328 1328
Size: 4289
KasI G'GCGC_C
Cuts at: 1719 1967 1719
Size: 248 4041
KpnI G_GTAC'C
Cuts at: 1309 1309
Size: 4289
MluI A'CGCG_T
Cuts at: 3722 3722
Size: 4289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2960 3144 2960
Size: 184 4105
MscI TGG'CCA
Cuts at: 2050 2050
Size: 4289
MslI CAynn'nnrTG
Cuts at: 2278 2405 2278
Size: 127 4162
MspA1I CmG'CkG
Cuts at: 519 1400 1714 2074 3087 3332 519
Size: 881 314 360 1013 245 1476
Fragments arranged by size:
1476 1013 881 360 314 245
NaeI GCC'GGC
Cuts at: 2470 2470
Size: 4289
NarI GG'CG_CC
Cuts at: 1720 1968 1720
Size: 248 4041
NcoI C'CATG_G
Cuts at: 107 615 107
Size: 508 3781
NdeI CA'TA_TG
Cuts at: 240 240
Size: 4289
NgoMIV G'CCGG_C
Cuts at: 2468 2468
Size: 4289
NsiI A_TGCA'T
Cuts at: 393 774 393
Size: 381 3908
NspI r_CATG'y
Cuts at: 772 1103 1342 1842 2749 772
Size: 331 239 500 907 2312
Fragments arranged by size:
2312 907 500 331 239
PciI A'CATG_T
Cuts at: 2745 2745
Size: 4289
PflMI CCAn_nnn'nTGG
Cuts at: 621 621
Size: 4289
PmeI GTTT'AAAC
Cuts at: 1288 1288
Size: 4289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 4257
PsiI TTA'TAA
Cuts at: 1277 1277
Size: 4289
PstI C_TGCA'G
Cuts at: 1336 2021 1336
Size: 685 3604
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4289
PvuII CAG'CTG
Cuts at: 519 1714 2074 519
Size: 1195 360 2734
Fragments arranged by size:
2734 1195 360
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 4289
SacI G_AGCT'C
Cuts at: 1303 1303
Size: 4289
SalI G'TCGA_C
Cuts at: 1326 1326
Size: 4289
SapI GCTCTTCn'nnn_
Cuts at: 421 715 2312 2522 421
Size: 294 1597 210 2188
Fragments arranged by size:
2188 1597 294 210
SbfI CC_TGCA'GG
Cuts at: 1336 1336
Size: 4289
ScaI AGT'ACT
Cuts at: 3806 3806
Size: 4289
SfcI C'TryA_G
Cuts at: 33 676 884 1332 2017 3010 3201 33
Size: 643 208 448 685 993 191 1121
Fragments arranged by size:
1121 993 685 643 448 208 191
SfoI GGC'GCC
Cuts at: 1721 1969 1721
Size: 248 4041
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
SmaI CCC'GGG
Cuts at: 1311 1311
Size: 4289
SmlI C'TyrA_G
Cuts at: 262 818 1444 2359 2851 3113 262
Size: 556 626 915 492 262 1438
Fragments arranged by size:
1438 915 626 556 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4289
SphI G_CATG'C
Cuts at: 772 1342 1842 772
Size: 570 500 3219
Fragments arranged by size:
3219 570 500
StuI AGG'CCT
Cuts at: 382 382
Size: 4289
StyI C'CwwG_G
Cuts at: 107 615 903 1423 1777 107
Size: 508 288 520 354 2619
Fragments arranged by size:
2619 520 508 354 288
SwaI ATTT'AAAT
Cuts at: 560 560
Size: 4289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1872 2005 2464 2673 1872
Size: 133 459 209 3488
Fragments arranged by size:
3488 459 209 133
TatI w'GTAC_w
Cuts at: 822 3804 822
Size: 2982 1307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 945 977 3806 3838 945
Size: 32 2829 32 1396
Fragments arranged by size:
2829 1396 32 32
Tth111I GACn'n_nGTC
Cuts at: 2086 2086
Size: 4289
XbaI T'CTAG_A
Cuts at: 1320 1320
Size: 4289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1784 114
Size: 1670 2619
XmaI C'CCGG_G
Cuts at: 1309 1309
Size: 4289
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BglI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII FseI
FspAI HindIII HpaI MfeI NheI NotI NruI PacI
PasI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
XhoI XmnI ZraI