Restriction map for
pF1KB6542
(Circular) (Six-base) MAPSORT of: pF1KB6542.seq Check: 3733 from: 1 to: 4286
pF1KB6542 4286 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 545 1324 545
Size: 779 3507
Acc65I G'GTAC_C
Cuts at: 1302 1302
Size: 4286
AclI AA'CG_TT
Cuts at: 4126 4126
Size: 4286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2543 3290 3558 2111
Size: 432 747 268 2839
Fragments arranged by size:
2839 747 432 268
AflIII A'CryG_T
Cuts at: 1638 2742 3719 1638
Size: 1104 977 2205
Fragments arranged by size:
2205 1104 977
AgeI A'CCGG_T
Cuts at: 2674 2674
Size: 4286
AleI CACnn'nnGTG
Cuts at: 359 359
Size: 4286
AlwNI CAG_nnn'CTG
Cuts at: 1591 3158 1591
Size: 1567 2719
ApaLI G'TGCA_C
Cuts at: 3056 3056
Size: 4286
ApoI r'AATT_y
Cuts at: 393 679 762 1290 3639 3895 393
Size: 286 83 528 2349 256 784
Fragments arranged by size:
2349 784 528 286 256 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 4286
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
AvaI C'yCGr_G
Cuts at: 368 1306 368
Size: 938 3348
BamHI G'GATC_C
Cuts at: 1311 1311
Size: 4286
BanI G'GyrC_C
Cuts at: 1302 1716 1964 1999 1302
Size: 414 248 35 3589
Fragments arranged by size:
3589 414 248 35
BanII G_rGCy'C
Cuts at: 203 521 935 1300 2330 203
Size: 318 414 365 1030 2159
Fragments arranged by size:
2159 1030 414 365 318
BbeI G_GCGC'C
Cuts at: 1720 1968 1720
Size: 248 4038
BbvCI CC'TCA_GC
Cuts at: 512 512
Size: 4286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 469 503 469
Size: 34 4252
BciVI GTATCCnnnnn_n'
Cuts at: 309 2184 2945 4286 309
Size: 1875 761 1341 309
Fragments arranged by size:
1875 1341 761 309
BclI T'GATC_A
Cuts at: 1806 1806
Size: 4286
BglII A'GATC_T
Cuts at: 1801 3838 1801
Size: 2037 2249
BlpI GC'TnA_GC
Cuts at: 1398 1398
Size: 4286
Bme1580I G_kGCm'C
Cuts at: 924 1911 2004 3060 924
Size: 987 93 1056 2150
Fragments arranged by size:
2150 1056 987 93
BmrI ACTGGGnnnn_n'
Cuts at: 1498 1579 1914 4058 1498
Size: 81 335 2144 1726
Fragments arranged by size:
2144 1726 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 638 2653 638
Size: 2015 2271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 214 646 1055 160
Size: 54 432 409 3391
Fragments arranged by size:
3391 432 409 54
Bpu10I CC'TnA_GC
Cuts at: 512 512
Size: 4286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1462 2341 2833 3131 208
Size: 1254 879 492 298 1363
Fragments arranged by size:
1363 1254 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 609 3990 609
Size: 3381 905
BsaAI yAC'GTr
Cuts at: 1639 1639
Size: 4286
BsaBI GATnn'nnATC
Cuts at: 6 1145 1800 3837 6
Size: 1139 655 2037 455
Fragments arranged by size:
2037 1139 655 455
BsaHI Gr'CG_yC
Cuts at: 1717 1965 2667 4163 1717
Size: 248 702 1496 1840
Fragments arranged by size:
1840 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1475 1996 2674 2948 3095 3506 1475
Size: 521 678 274 147 411 2255
Fragments arranged by size:
2255 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 529 211
Size: 318 3968
BseYI C'CCAG_C
Cuts at: 1711 2469 3046 3542 1711
Size: 758 577 496 2455
Fragments arranged by size:
2455 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 136
Size: 4286
BsiEI CG_ry'CG
Cuts at: 104 1828 1874 3082 104
Size: 1724 46 1208 1308
Fragments arranged by size:
1724 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 203 482 521 1300 2078 2268 3060 203
Size: 279 39 779 778 190 792 1429
Fragments arranged by size:
1429 792 779 778 279 190 39
BsmI GAATG_Cn'
Cuts at: 503 503
Size: 4286
Bsp1286I G_dGCh'C
Cuts at: 203 482 521 924 935 1300 1911 2004 2078
Size: 279 39 403 11 365 611 93 74
Cuts at: 2078 2268 2330 3060 203
Size: 190 62 730 1429
Fragments arranged by size:
1429 730 611 403 365 279 190 93
74 62 39 11
BspEI T'CCGG_A
Cuts at: 3506 3506
Size: 4286
BspHI T'CATG_A
Cuts at: 3462 3462
Size: 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 627 961 1336 1550 1852 2233 627
Size: 334 375 214 302 381 2680
Fragments arranged by size:
2680 381 375 334 302 214
BsrBI CCG'CTC
Cuts at: 50 2578 2632 4116 4281 50
Size: 2528 54 1484 165 55
Fragments arranged by size:
2528 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2198 2198
Size: 4286
BsrFI r'CCGG_y
Cuts at: 214 2284 2465 2674 214
Size: 2070 181 209 1826
Fragments arranged by size:
2070 1826 209 181
BssSI C'ACGA_G
Cuts at: 229 2557 2915 229
Size: 2328 358 1600
Fragments arranged by size:
2328 1600 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1663 1663
Size: 4286
BstBI TT'CG_AA
Cuts at: 2647 2647
Size: 4286
BstXI CCAn_nnnn'nTGG
Cuts at: 1161 1161
Size: 4286
BstYI r'GATC_y
Cuts at: 1 233 1311 1778 1801 2136 2382 3394 3480
Size: 232 1078 467 23 335 246 1012 86
Cuts at: 3480 3492 3838 1
Size: 12 346 449
Fragments arranged by size:
1078 1012 467 449 346 335 246 232
86 23 12
BtgI C'CryG_G
Cuts at: 107 246 938 107
Size: 139 692 3455
Fragments arranged by size:
3455 692 139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2236 2417 2580 2236
Size: 181 163 3942
Fragments arranged by size:
3942 181 163
BtsI GCAGTG_nn'
Cuts at: 366 420 641 1033 1529 366
Size: 54 221 392 496 3123
Fragments arranged by size:
3123 496 392 221 54
DraI TTT'AAA
Cuts at: 1285 1285
Size: 4286
DrdI GACnn_nn'nnGTC
Cuts at: 1992 2850 1992
Size: 858 3428
EaeI y'GGCC_r
Cuts at: 249 1871 2045 2436 2463 3808 249
Size: 1622 174 391 27 1345 727
Fragments arranged by size:
1622 1345 727 391 174 27
EagI C'GGCC_G
Cuts at: 1871 1871
Size: 4286
EarI CTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2494 2804 2950 4096 2494
Size: 310 146 1146 2684
Fragments arranged by size:
2684 1146 310 146
EcoICRI GAG'CTC
Cuts at: 201 519 1298 201
Size: 318 779 3189
Fragments arranged by size:
3189 779 318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 214 646 1055 2111 2543 3290 3558 160
Size: 54 432 409 1056 432 747 268 888
Fragments arranged by size:
1056 888 747 432 432 409 268 54
EcoO109I rG'GnC_Cy
Cuts at: 1425 1425
Size: 4286
EcoRI G'AATT_C
Cuts at: 1290 1290
Size: 4286
EcoRV GAT'ATC
Cuts at: 891 891
Size: 4286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1093 2305 1093
Size: 1212 3074
FspI TGC'GCA
Cuts at: 2067 2067
Size: 4286
HaeII r_GCGC'y
Cuts at: 985 1720 1968 2990 3969 985
Size: 735 248 1022 979 1302
Fragments arranged by size:
1302 1022 979 735 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2128 2158 2160 2190 2128
Size: 30 2 30 4224
Fragments arranged by size:
4224 30 30 2
HincII GTy'rAC
Cuts at: 1325 1325
Size: 4286
KasI G'GCGC_C
Cuts at: 1716 1964 1716
Size: 248 4038
KpnI G_GTAC'C
Cuts at: 1306 1306
Size: 4286
MluI A'CGCG_T
Cuts at: 3719 3719
Size: 4286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 354 362 2957 3141 354
Size: 8 2595 184 1499
Fragments arranged by size:
2595 1499 184 8
MscI TGG'CCA
Cuts at: 2047 2047
Size: 4286
MslI CAynn'nnrTG
Cuts at: 359 2275 2402 359
Size: 1916 127 2243
Fragments arranged by size:
2243 1916 127
MspA1I CmG'CkG
Cuts at: 177 1397 1711 2071 3084 3329 177
Size: 1220 314 360 1013 245 1134
Fragments arranged by size:
1220 1134 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2467 2467
Size: 4286
NarI GG'CG_CC
Cuts at: 1717 1965 1717
Size: 248 4038
NcoI C'CATG_G
Cuts at: 107 107
Size: 4286
NdeI CA'TA_TG
Cuts at: 855 855
Size: 4286
NgoMIV G'CCGG_C
Cuts at: 2465 2465
Size: 4286
NspI r_CATG'y
Cuts at: 1339 1839 2746 1339
Size: 500 907 2879
Fragments arranged by size:
2879 907 500
PacI TTA_AT'TAA
Cuts at: 998 998
Size: 4286
PciI A'CATG_T
Cuts at: 2742 2742
Size: 4286
PfoI T'CCnGG_A
Cuts at: 1254 1254
Size: 4286
PmeI GTTT'AAAC
Cuts at: 1285 1285
Size: 4286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 4254
PshAI GACnn'nnGTC
Cuts at: 551 551
Size: 4286
PstI C_TGCA'G
Cuts at: 242 1333 2018 242
Size: 1091 685 2510
Fragments arranged by size:
2510 1091 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4286
PvuII CAG'CTG
Cuts at: 177 1711 2071 177
Size: 1534 360 2392
Fragments arranged by size:
2392 1534 360
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 4286
SacI G_AGCT'C
Cuts at: 203 521 1300 203
Size: 318 779 3189
Fragments arranged by size:
3189 779 318
SalI G'TCGA_C
Cuts at: 1323 1323
Size: 4286
SapI GCTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
SbfI CC_TGCA'GG
Cuts at: 1333 1333
Size: 4286
ScaI AGT'ACT
Cuts at: 3803 3803
Size: 4286
SfcI C'TryA_G
Cuts at: 33 238 542 1329 2014 3007 3198 33
Size: 205 304 787 685 993 191 1121
Fragments arranged by size:
1121 993 787 685 304 205 191
SfoI GGC'GCC
Cuts at: 1718 1966 1718
Size: 248 4038
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
SgrAI Cr'CCGG_yG
Cuts at: 214 214
Size: 4286
SmaI CCC'GGG
Cuts at: 1308 1308
Size: 4286
SmlI C'TyrA_G
Cuts at: 223 1441 2356 2848 3110 223
Size: 1218 915 492 262 1399
Fragments arranged by size:
1399 1218 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4286
SphI G_CATG'C
Cuts at: 1339 1839 1339
Size: 500 3786
SspI AAT'ATT
Cuts at: 587 587
Size: 4286
StyI C'CwwG_G
Cuts at: 107 674 1077 1420 1774 107
Size: 567 403 343 354 2619
Fragments arranged by size:
2619 567 403 354 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 709 1167 1869 2002 2461 2670 709
Size: 458 702 133 459 209 2325
Fragments arranged by size:
2325 702 459 458 209 133
TatI w'GTAC_w
Cuts at: 1030 3801 1030
Size: 2771 1515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 806 838 3803 3835 806
Size: 32 2965 32 1257
Fragments arranged by size:
2965 1257 32 32
Tth111I GACn'n_nGTC
Cuts at: 2083 2083
Size: 4286
XbaI T'CTAG_A
Cuts at: 1317 1317
Size: 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1781 1781
Size: 4286
XmaI C'CCGG_G
Cuts at: 1306 1306
Size: 4286
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PacI PciI PfoI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PasI PflMI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI StuI SwaI XhoI XmnI ZraI