Restriction map for
pF1KB6528
(Circular) (Six-base) MAPSORT of: pF1KB6528.seq Check: 3186 from: 1 to: 4277
pF1KB6528 4277 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 539 539
Size: 4277
AccI GT'mk_AC
Cuts at: 1315 1315
Size: 4277
Acc65I G'GTAC_C
Cuts at: 1293 1293
Size: 4277
AclI AA'CG_TT
Cuts at: 4117 4117
Size: 4277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 2534 3281 3549 2102
Size: 432 747 268 2830
Fragments arranged by size:
2830 747 432 268
AflIII A'CryG_T
Cuts at: 580 621 1629 2733 3710 580
Size: 41 1008 1104 977 1147
Fragments arranged by size:
1147 1104 1008 977 41
AgeI A'CCGG_T
Cuts at: 2665 2665
Size: 4277
AleI CACnn'nnGTG
Cuts at: 622 622
Size: 4277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1268 1268
Size: 4277
AlwNI CAG_nnn'CTG
Cuts at: 450 1582 3149 450
Size: 1132 1567 1578
Fragments arranged by size:
1578 1567 1132
ApaLI G'TGCA_C
Cuts at: 3047 3047
Size: 4277
ApoI r'AATT_y
Cuts at: 697 1093 1281 3630 3886 697
Size: 396 188 2349 256 1088
Fragments arranged by size:
2349 1088 396 256 188
AseI AT'TA_AT
Cuts at: 20 20
Size: 4277
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
AvaI C'yCGr_G
Cuts at: 457 1297 457
Size: 840 3437
BamHI G'GATC_C
Cuts at: 1302 1302
Size: 4277
BanI G'GyrC_C
Cuts at: 520 599 1293 1707 1955 1990 520
Size: 79 694 414 248 35 2807
Fragments arranged by size:
2807 694 414 248 79 35
BanII G_rGCy'C
Cuts at: 197 1291 2321 197
Size: 1094 1030 2153
Fragments arranged by size:
2153 1094 1030
BbeI G_GCGC'C
Cuts at: 1711 1959 1711
Size: 248 4029
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1044 1078 1044
Size: 34 4243
BciVI GTATCCnnnnn_n'
Cuts at: 701 2175 2936 4277 701
Size: 1474 761 1341 701
Fragments arranged by size:
1474 1341 761 701
BclI T'GATC_A
Cuts at: 1115 1797 1115
Size: 682 3595
BglI GCCn_nnn'nGGC
Cuts at: 130 130
Size: 4277
BglII A'GATC_T
Cuts at: 1792 3829 1792
Size: 2037 2240
BlpI GC'TnA_GC
Cuts at: 1389 1389
Size: 4277
Bme1580I G_kGCm'C
Cuts at: 457 604 1902 1995 3051 457
Size: 147 1298 93 1056 1683
Fragments arranged by size:
1683 1298 1056 147 93
BmgBI CAC'GTC
Cuts at: 581 581
Size: 4277
BmrI ACTGGGnnnn_n'
Cuts at: 891 1489 1570 1905 4049 891
Size: 598 81 335 2144 1119
Fragments arranged by size:
2144 1119 598 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 414 2644 414
Size: 2230 2047
Bpu10I CC'TnA_GC
Cuts at: 386 386
Size: 4277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1453 2332 2824 3122 733
Size: 720 879 492 298 1888
Fragments arranged by size:
1888 879 720 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1004 3981 1004
Size: 2977 1300
BsaAI yAC'GTr
Cuts at: 624 1630 624
Size: 1006 3271
BsaBI GATnn'nnATC
Cuts at: 6 1120 1791 3828 6
Size: 1114 671 2037 455
Fragments arranged by size:
2037 1114 671 455
BsaHI Gr'CG_yC
Cuts at: 1708 1956 2658 4154 1708
Size: 248 702 1496 1831
Fragments arranged by size:
1831 1496 702 248
BsaWI w'CCGG_w
Cuts at: 350 1466 1987 2665 2939 3086 3497 350
Size: 1116 521 678 274 147 411 1130
Fragments arranged by size:
1130 1116 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1702 2460 3037 3533 1702
Size: 758 577 496 2446
Fragments arranged by size:
2446 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1157 1819 1865 3073 104
Size: 1053 662 46 1208 1308
Fragments arranged by size:
1308 1208 1053 662 46
BsiHKAI G_wGCw'C
Cuts at: 173 965 1291 2069 2259 3051 173
Size: 792 326 778 190 792 1399
Fragments arranged by size:
1399 792 792 778 326 190
Bsp1286I G_dGCh'C
Cuts at: 173 197 457 604 965 1291 1902 1995 2069
Size: 24 260 147 361 326 611 93 74
Cuts at: 2069 2259 2321 3051 173
Size: 190 62 730 1399
Fragments arranged by size:
1399 730 611 361 326 260 190 147
93 74 62 24
BspEI T'CCGG_A
Cuts at: 350 3497 350
Size: 3147 1130
BspHI T'CATG_A
Cuts at: 197 410 3453 197
Size: 213 3043 1021
Fragments arranged by size:
3043 1021 213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 539 642 1327 1541 1843 2224 539
Size: 103 685 214 302 381 2592
Fragments arranged by size:
2592 685 381 302 214 103
BsrBI CCG'CTC
Cuts at: 50 2569 2623 4107 4272 50
Size: 2519 54 1484 165 55
Fragments arranged by size:
2519 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2189 2189
Size: 4277
BsrFI r'CCGG_y
Cuts at: 508 517 2275 2456 2665 508
Size: 9 1758 181 209 2120
Fragments arranged by size:
2120 1758 209 181 9
BssSI C'ACGA_G
Cuts at: 2548 2906 2548
Size: 358 3919
BstAPI GCAn_nnn'nTGC
Cuts at: 1654 1654
Size: 4277
BstBI TT'CG_AA
Cuts at: 2638 2638
Size: 4277
BstYI r'GATC_y
Cuts at: 1 353 777 1302 1769 1792 2127 2373 3385
Size: 352 424 525 467 23 335 246 1012
Cuts at: 3385 3471 3483 3829 1
Size: 86 12 346 449
Fragments arranged by size:
1012 525 467 449 424 352 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 284 848 107
Size: 177 564 3536
Fragments arranged by size:
3536 564 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2227 2408 2571 2227
Size: 181 163 3933
Fragments arranged by size:
3933 181 163
BtsI GCAGTG_nn'
Cuts at: 1138 1520 1138
Size: 382 3895
DraI TTT'AAA
Cuts at: 644 1276 644
Size: 632 3645
DraIII CAC_nnn'GTG
Cuts at: 482 482
Size: 4277
DrdI GACnn_nn'nnGTC
Cuts at: 286 1174 1983 2841 286
Size: 888 809 858 1722
Fragments arranged by size:
1722 888 858 809
EaeI y'GGCC_r
Cuts at: 845 1862 2036 2427 2454 3799 845
Size: 1017 174 391 27 1345 1323
Fragments arranged by size:
1345 1323 1017 391 174 27
EagI C'GGCC_G
Cuts at: 1862 1862
Size: 4277
EarI CTCTTCn'nnn_
Cuts at: 409 2300 2510 409
Size: 1891 210 2176
Fragments arranged by size:
2176 1891 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 382 925 2485 2795 2941 4087 382
Size: 543 1560 310 146 1146 572
Fragments arranged by size:
1560 1146 572 543 310 146
EcoICRI GAG'CTC
Cuts at: 1289 1289
Size: 4277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 2534 3281 3549 2102
Size: 432 747 268 2830
Fragments arranged by size:
2830 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 447 1416 447
Size: 969 3308
EcoRI G'AATT_C
Cuts at: 1281 1281
Size: 4277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 4277
FspI TGC'GCA
Cuts at: 2058 2058
Size: 4277
HaeII r_GCGC'y
Cuts at: 1711 1959 2981 3960 1711
Size: 248 1022 979 2028
Fragments arranged by size:
2028 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 338 369 370 401 2119 2149 2151 2181 338
Size: 31 1 31 1718 30 2 30 2434
Fragments arranged by size:
2434 1718 31 31 30 30 2 1
HincII GTy'rAC
Cuts at: 879 1316 879
Size: 437 3840
KasI G'GCGC_C
Cuts at: 1707 1955 1707
Size: 248 4029
KpnI G_GTAC'C
Cuts at: 1297 1297
Size: 4277
MluI A'CGCG_T
Cuts at: 3710 3710
Size: 4277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2948 3132 2948
Size: 184 4093
MscI TGG'CCA
Cuts at: 2038 2038
Size: 4277
MslI CAynn'nnrTG
Cuts at: 622 847 1072 2266 2393 622
Size: 225 225 1194 127 2506
Fragments arranged by size:
2506 1194 225 225 127
MspA1I CmG'CkG
Cuts at: 500 1388 1702 2062 3075 3320 500
Size: 888 314 360 1013 245 1457
Fragments arranged by size:
1457 1013 888 360 314 245
NaeI GCC'GGC
Cuts at: 510 519 2458 510
Size: 9 1939 2329
Fragments arranged by size:
2329 1939 9
NarI GG'CG_CC
Cuts at: 1708 1956 1708
Size: 248 4029
NcoI C'CATG_G
Cuts at: 107 107
Size: 4277
NgoMIV G'CCGG_C
Cuts at: 508 517 2456 508
Size: 9 1939 2329
Fragments arranged by size:
2329 1939 9
NspI r_CATG'y
Cuts at: 1330 1830 2737 1330
Size: 500 907 2870
Fragments arranged by size:
2870 907 500
PciI A'CATG_T
Cuts at: 2733 2733
Size: 4277
PflMI CCAn_nnn'nTGG
Cuts at: 843 843
Size: 4277
PfoI T'CCnGG_A
Cuts at: 836 836
Size: 4277
PmeI GTTT'AAAC
Cuts at: 1276 1276
Size: 4277
PmlI CAC'GTG
Cuts at: 624 624
Size: 4277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 4245
PshAI GACnn'nnGTC
Cuts at: 307 307
Size: 4277
PstI C_TGCA'G
Cuts at: 654 1324 2009 654
Size: 670 685 2922
Fragments arranged by size:
2922 685 670
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4277
PvuII CAG'CTG
Cuts at: 500 1702 2062 500
Size: 1202 360 2715
Fragments arranged by size:
2715 1202 360
RsrII CG'GwC_CG
Cuts at: 2472 2472
Size: 4277
SacI G_AGCT'C
Cuts at: 1291 1291
Size: 4277
SalI G'TCGA_C
Cuts at: 1314 1314
Size: 4277
SapI GCTCTTCn'nnn_
Cuts at: 2300 2510 2300
Size: 210 4067
SbfI CC_TGCA'GG
Cuts at: 1324 1324
Size: 4277
ScaI AGT'ACT
Cuts at: 1003 3794 1003
Size: 2791 1486
SexAI A'CCwGG_T
Cuts at: 1268 1268
Size: 4277
SfcI C'TryA_G
Cuts at: 33 650 1005 1320 2005 2998 3189 33
Size: 617 355 315 685 993 191 1121
Fragments arranged by size:
1121 993 685 617 355 315 191
SfoI GGC'GCC
Cuts at: 1709 1957 1709
Size: 248 4029
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
SmaI CCC'GGG
Cuts at: 1299 1299
Size: 4277
SmlI C'TyrA_G
Cuts at: 748 1432 2347 2839 3101 748
Size: 684 915 492 262 1924
Fragments arranged by size:
1924 915 684 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4277
SphI G_CATG'C
Cuts at: 1330 1830 1330
Size: 500 3777
StuI AGG'CCT
Cuts at: 1048 1048
Size: 4277
StyI C'CwwG_G
Cuts at: 107 305 1411 1765 107
Size: 198 1106 354 2619
Fragments arranged by size:
2619 1106 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 234 538 1860 1993 2452 2661 234
Size: 304 1322 133 459 209 1850
Fragments arranged by size:
1850 1322 459 304 209 133
TatI w'GTAC_w
Cuts at: 502 1001 3792 502
Size: 499 2791 987
Fragments arranged by size:
2791 987 499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4245
Tth111I GACn'n_nGTC
Cuts at: 2074 2074
Size: 4277
XbaI T'CTAG_A
Cuts at: 1308 1308
Size: 4277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1772 1772
Size: 4277
XmaI C'CCGG_G
Cuts at: 1297 1297
Size: 4277
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI BpmI BsaXI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI