Restriction map for
pF1KB6527
(Circular) (Six-base) MAPSORT of: pF1KB6527.seq Check: 2816 from: 1 to: 4208
pF1KB6527 4208 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1042 1042
Size: 4208
AatII G_ACGT'C
Cuts at: 947 947
Size: 4208
AccI GT'mk_AC
Cuts at: 1246 1246
Size: 4208
Acc65I G'GTAC_C
Cuts at: 1224 1224
Size: 4208
AclI AA'CG_TT
Cuts at: 4048 4048
Size: 4208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1075 1147 1159 2033 2465 3212 3480 739
Size: 336 72 12 874 432 747 268 1467
Fragments arranged by size:
1467 874 747 432 336 268 72 12
AfeI AGC'GCT
Cuts at: 229 229
Size: 4208
AflIII A'CryG_T
Cuts at: 1560 2664 3641 1560
Size: 1104 977 2127
Fragments arranged by size:
2127 1104 977
AgeI A'CCGG_T
Cuts at: 2596 2596
Size: 4208
AleI CACnn'nnGTG
Cuts at: 563 563
Size: 4208
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 365 397 365
Size: 32 4176
AlwNI CAG_nnn'CTG
Cuts at: 1513 3080 1513
Size: 1567 2641
ApaLI G'TGCA_C
Cuts at: 2978 2978
Size: 4208
ApoI r'AATT_y
Cuts at: 1212 3561 3817 1212
Size: 2349 256 1603
Fragments arranged by size:
2349 1603 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4208
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
AvaI C'yCGr_G
Cuts at: 331 468 1228 331
Size: 137 760 3311
Fragments arranged by size:
3311 760 137
BamHI G'GATC_C
Cuts at: 409 1233 409
Size: 824 3384
BanI G'GyrC_C
Cuts at: 155 464 605 878 1224 1638 1886 1921 155
Size: 309 141 273 346 414 248 35 2442
Fragments arranged by size:
2442 414 346 309 273 248 141 35
BanII G_rGCy'C
Cuts at: 175 341 731 1222 2252 175
Size: 166 390 491 1030 2131
Fragments arranged by size:
2131 1030 491 390 166
BbeI G_GCGC'C
Cuts at: 159 1642 1890 159
Size: 1483 248 2477
Fragments arranged by size:
2477 1483 248
BciVI GTATCCnnnnn_n'
Cuts at: 2106 2867 4208 2106
Size: 761 1341 2106
Fragments arranged by size:
2106 1341 761
BclI T'GATC_A
Cuts at: 1728 1728
Size: 4208
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4208
BglII A'GATC_T
Cuts at: 1723 3760 1723
Size: 2037 2171
BlpI GC'TnA_GC
Cuts at: 1320 1320
Size: 4208
Bme1580I G_kGCm'C
Cuts at: 312 881 1833 1926 2982 312
Size: 569 952 93 1056 1538
Fragments arranged by size:
1538 1056 952 569 93
BmrI ACTGGGnnnn_n'
Cuts at: 306 1420 1501 1836 3980 306
Size: 1114 81 335 2144 534
Fragments arranged by size:
2144 1114 534 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1176 2575 1176
Size: 1399 2809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1141
Size: 4208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 2263 2755 3053 1384
Size: 879 492 298 2539
Fragments arranged by size:
2539 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3912 3912
Size: 4208
BsaAI yAC'GTr
Cuts at: 834 1561 834
Size: 727 3481
BsaBI GATnn'nnATC
Cuts at: 6 1722 3759 6
Size: 1716 2037 455
Fragments arranged by size:
2037 1716 455
BsaHI Gr'CG_yC
Cuts at: 156 203 650 761 944 1639 1887 2589 4085
Size: 47 447 111 183 695 248 702 1496
Cuts at: 4085 156
Size: 279
Fragments arranged by size:
1496 702 695 447 279 248 183 111
47
BsaWI w'CCGG_w
Cuts at: 1397 1918 2596 2870 3017 3428 1397
Size: 521 678 274 147 411 2177
Fragments arranged by size:
2177 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 120 816 1172 1633 2391 2968 3464 120
Size: 696 356 461 758 577 496 864
Fragments arranged by size:
864 758 696 577 496 461 356
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 660
Size: 4208
BsiEI CG_ry'CG
Cuts at: 104 1750 1796 3004 104
Size: 1646 46 1208 1308
Fragments arranged by size:
1646 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 296 731 842 1222 2000 2190 2982 296
Size: 435 111 380 778 190 792 1522
Fragments arranged by size:
1522 792 778 435 380 190 111
BsmI GAATG_Cn'
Cuts at: 534 534
Size: 4208
Bsp1286I G_dGCh'C
Cuts at: 175 296 312 341 731 842 881 1222 1833
Size: 121 16 29 390 111 39 341 611
Cuts at: 1833 1926 2000 2190 2252 2982 175
Size: 93 74 190 62 730 1401
Fragments arranged by size:
1401 730 611 390 341 190 121 111
93 74 62 39 29 16
BspEI T'CCGG_A
Cuts at: 3428 3428
Size: 4208
BspHI T'CATG_A
Cuts at: 3384 3384
Size: 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1042 1258 1472 1774 2155 1042
Size: 216 214 302 381 3095
Fragments arranged by size:
3095 381 302 216 214
BsrBI CCG'CTC
Cuts at: 50 2500 2554 4038 4203 50
Size: 2450 54 1484 165 55
Fragments arranged by size:
2450 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 279 2120 279
Size: 1841 2367
BsrFI r'CCGG_y
Cuts at: 149 164 532 548 785 881 2206 2387 2596
Size: 15 368 16 237 96 1325 181 209
Cuts at: 2596 149
Size: 1761
Fragments arranged by size:
1761 1325 368 237 209 181 96 16
15
BssSI C'ACGA_G
Cuts at: 2479 2837 2479
Size: 358 3850
BstAPI GCAn_nnn'nTGC
Cuts at: 1585 1585
Size: 4208
BstBI TT'CG_AA
Cuts at: 2569 2569
Size: 4208
BstXI CCAn_nnnn'nTGG
Cuts at: 582 582
Size: 4208
BstYI r'GATC_y
Cuts at: 1 409 1233 1700 1723 2058 2304 3316 3402
Size: 408 824 467 23 335 246 1012 86
Cuts at: 3402 3414 3760 1
Size: 12 346 449
Fragments arranged by size:
1012 824 467 449 408 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 185 107
Size: 78 4130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 992 2158 2339 2502 992
Size: 1166 181 163 2698
Fragments arranged by size:
2698 1166 181 163
BtsI GCAGTG_nn'
Cuts at: 294 832 1451 294
Size: 538 619 3051
Fragments arranged by size:
3051 619 538
DraI TTT'AAA
Cuts at: 1207 1207
Size: 4208
DrdI GACnn_nn'nnGTC
Cuts at: 1914 2772 1914
Size: 858 3350
EaeI y'GGCC_r
Cuts at: 131 209 432 1793 1967 2358 2385 3730 131
Size: 78 223 1361 174 391 27 1345 609
Fragments arranged by size:
1361 1345 609 391 223 174 78 27
EagI C'GGCC_G
Cuts at: 1793 1793
Size: 4208
EarI CTCTTCn'nnn_
Cuts at: 346 940 2231 2441 346
Size: 594 1291 210 2113
Fragments arranged by size:
2113 1291 594 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2416 2726 2872 4018 2416
Size: 310 146 1146 2606
Fragments arranged by size:
2606 1146 310 146
EcoICRI GAG'CTC
Cuts at: 729 1220 729
Size: 491 3717
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1075 1141 1147 1159 2033 2465 3212 3480
Size: 336 66 6 12 874 432 747 268
Cuts at: 3480 739
Size: 1467
Fragments arranged by size:
1467 874 747 432 336 268 66 12
6
EcoNI CCTnn'n_nnAGG
Cuts at: 997 997
Size: 4208
EcoO109I rG'GnC_Cy
Cuts at: 579 776 900 1347 579
Size: 197 124 447 3440
Fragments arranged by size:
3440 447 197 124
EcoRI G'AATT_C
Cuts at: 1212 1212
Size: 4208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 4208
FspI TGC'GCA
Cuts at: 1989 1989
Size: 4208
HaeII r_GCGC'y
Cuts at: 159 231 1642 1890 2912 3891 159
Size: 72 1411 248 1022 979 476
Fragments arranged by size:
1411 1022 979 476 248 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 970 1002 1180 1212 2050 2080 2082 2112 970
Size: 32 178 32 838 30 2 30 3066
Fragments arranged by size:
3066 838 178 32 32 30 30 2
HincII GTy'rAC
Cuts at: 267 810 1247 267
Size: 543 437 3228
Fragments arranged by size:
3228 543 437
KasI G'GCGC_C
Cuts at: 155 1638 1886 155
Size: 1483 248 2477
Fragments arranged by size:
2477 1483 248
KpnI G_GTAC'C
Cuts at: 1228 1228
Size: 4208
MluI A'CGCG_T
Cuts at: 3641 3641
Size: 4208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 179 1130 2879 3063 179
Size: 951 1749 184 1324
Fragments arranged by size:
1749 1324 951 184
MscI TGG'CCA
Cuts at: 211 1969 211
Size: 1758 2450
MslI CAynn'nnrTG
Cuts at: 563 2197 2324 563
Size: 1634 127 2447
Fragments arranged by size:
2447 1634 127
MspA1I CmG'CkG
Cuts at: 482 603 1119 1319 1633 1993 3006 3251 482
Size: 121 516 200 314 360 1013 245 1439
Fragments arranged by size:
1439 1013 516 360 314 245 200 121
NaeI GCC'GGC
Cuts at: 534 550 787 2389 534
Size: 16 237 1602 2353
Fragments arranged by size:
2353 1602 237 16
NarI GG'CG_CC
Cuts at: 156 1639 1887 156
Size: 1483 248 2477
Fragments arranged by size:
2477 1483 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4208
NgoMIV G'CCGG_C
Cuts at: 532 548 785 2387 532
Size: 16 237 1602 2353
Fragments arranged by size:
2353 1602 237 16
NspI r_CATG'y
Cuts at: 1261 1761 2668 1261
Size: 500 907 2801
Fragments arranged by size:
2801 907 500
PciI A'CATG_T
Cuts at: 2664 2664
Size: 4208
PmeI GTTT'AAAC
Cuts at: 1207 1207
Size: 4208
PmlI CAC'GTG
Cuts at: 834 834
Size: 4208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 4176
PstI C_TGCA'G
Cuts at: 961 1255 1940 961
Size: 294 685 3229
Fragments arranged by size:
3229 685 294
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4208
PvuII CAG'CTG
Cuts at: 1633 1993 1633
Size: 360 3848
RsrII CG'GwC_CG
Cuts at: 2403 2403
Size: 4208
SacI G_AGCT'C
Cuts at: 731 1222 731
Size: 491 3717
SalI G'TCGA_C
Cuts at: 1245 1245
Size: 4208
SapI GCTCTTCn'nnn_
Cuts at: 346 940 2231 2441 346
Size: 594 1291 210 2113
Fragments arranged by size:
2113 1291 594 210
SbfI CC_TGCA'GG
Cuts at: 1255 1255
Size: 4208
ScaI AGT'ACT
Cuts at: 367 3725 367
Size: 3358 850
SexAI A'CCwGG_T
Cuts at: 611 611
Size: 4208
SfcI C'TryA_G
Cuts at: 33 957 1251 1936 2929 3120 33
Size: 924 294 685 993 191 1121
Fragments arranged by size:
1121 993 924 685 294 191
SfoI GGC'GCC
Cuts at: 157 1640 1888 157
Size: 1483 248 2477
Fragments arranged by size:
2477 1483 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
SmaI CCC'GGG
Cuts at: 470 1230 470
Size: 760 3448
SmlI C'TyrA_G
Cuts at: 1363 2278 2770 3032 1363
Size: 915 492 262 2539
Fragments arranged by size:
2539 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4208
SphI G_CATG'C
Cuts at: 1261 1761 1261
Size: 500 3708
StuI AGG'CCT
Cuts at: 1003 1003
Size: 4208
StyI C'CwwG_G
Cuts at: 107 206 575 1178 1342 1696 107
Size: 99 369 603 164 354 2619
Fragments arranged by size:
2619 603 369 354 164 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 112 694 1791 1924 2383 2592 112
Size: 582 1097 133 459 209 1728
Fragments arranged by size:
1728 1097 582 459 209 133
TatI w'GTAC_w
Cuts at: 365 590 3723 365
Size: 225 3133 850
Fragments arranged by size:
3133 850 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3725 3757 3725
Size: 32 4176
Tth111I GACn'n_nGTC
Cuts at: 2005 2005
Size: 4208
XbaI T'CTAG_A
Cuts at: 1239 1239
Size: 4208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1703 1703
Size: 4208
XmaI C'CCGG_G
Cuts at: 468 1228 468
Size: 760 3448
XmnI GAAnn'nnTTC
Cuts at: 868 868
Size: 4208
ZraI GAC'GTC
Cuts at: 945 945
Size: 4208
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AlfI ApaI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI