Restriction map for
pF1KB6525
(Circular) (Six-base) MAPSORT of: pF1KB6525.seq Check: 7211 from: 1 to: 4208
pF1KB6525 4208 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1246 1246
Size: 4208
Acc65I G'GTAC_C
Cuts at: 1224 1224
Size: 4208
AclI AA'CG_TT
Cuts at: 4048 4048
Size: 4208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 2033 2465 3212 3480 748
Size: 1285 432 747 268 1476
Fragments arranged by size:
1476 1285 747 432 268
AflIII A'CryG_T
Cuts at: 1560 2664 3641 1560
Size: 1104 977 2127
Fragments arranged by size:
2127 1104 977
AgeI A'CCGG_T
Cuts at: 2596 2596
Size: 4208
AleI CACnn'nnGTG
Cuts at: 487 487
Size: 4208
AlwNI CAG_nnn'CTG
Cuts at: 282 453 1048 1513 3080 282
Size: 171 595 465 1567 1410
Fragments arranged by size:
1567 1410 595 465 171
ApaI G_GGCC'C
Cuts at: 271 475 271
Size: 204 4004
ApaLI G'TGCA_C
Cuts at: 2978 2978
Size: 4208
ApoI r'AATT_y
Cuts at: 531 1212 3561 3817 531
Size: 681 2349 256 922
Fragments arranged by size:
2349 922 681 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4208
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
AvaI C'yCGr_G
Cuts at: 231 571 1228 231
Size: 340 657 3211
Fragments arranged by size:
3211 657 340
BamHI G'GATC_C
Cuts at: 871 1233 871
Size: 362 3846
BanI G'GyrC_C
Cuts at: 489 550 835 1224 1638 1886 1921 489
Size: 61 285 389 414 248 35 2776
Fragments arranged by size:
2776 414 389 285 248 61 35
BanII G_rGCy'C
Cuts at: 146 271 317 475 632 713 1062 1222 2252
Size: 125 46 158 157 81 349 160 1030
Cuts at: 2252 146
Size: 2102
Fragments arranged by size:
2102 1030 349 160 158 157 125 81
46
BbeI G_GCGC'C
Cuts at: 1642 1890 1642
Size: 248 3960
BbvCI CC'TCA_GC
Cuts at: 125 125
Size: 4208
BciVI GTATCCnnnnn_n'
Cuts at: 2106 2867 4208 2106
Size: 761 1341 2106
Fragments arranged by size:
2106 1341 761
BclI T'GATC_A
Cuts at: 1728 1728
Size: 4208
BglI GCCn_nnn'nGGC
Cuts at: 302 458 302
Size: 156 4052
BglII A'GATC_T
Cuts at: 375 1723 3760 375
Size: 1348 2037 823
Fragments arranged by size:
2037 1348 823
BlpI GC'TnA_GC
Cuts at: 1320 1320
Size: 4208
Bme1580I G_kGCm'C
Cuts at: 271 475 494 553 1833 1926 2982 271
Size: 204 19 59 1280 93 1056 1497
Fragments arranged by size:
1497 1280 1056 204 93 59 19
BmrI ACTGGGnnnn_n'
Cuts at: 478 1420 1501 1836 3980 478
Size: 942 81 335 2144 706
Fragments arranged by size:
2144 942 706 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 732 2575 732
Size: 1843 2365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 718 968 697
Size: 21 250 3937
Fragments arranged by size:
3937 250 21
Bpu10I CC'TnA_GC
Cuts at: 125 283 125
Size: 158 4050
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1384 2263 2755 3053 526
Size: 858 879 492 298 1681
Fragments arranged by size:
1681 879 858 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 361 3912 361
Size: 3551 657
BsaAI yAC'GTr
Cuts at: 1561 1561
Size: 4208
BsaBI GATnn'nnATC
Cuts at: 6 1722 3759 6
Size: 1716 2037 455
Fragments arranged by size:
2037 1716 455
BsaHI Gr'CG_yC
Cuts at: 1639 1887 2589 4085 1639
Size: 248 702 1496 1762
Fragments arranged by size:
1762 1496 702 248
BsaWI w'CCGG_w
Cuts at: 792 1397 1918 2596 2870 3017 3428 792
Size: 605 521 678 274 147 411 1572
Fragments arranged by size:
1572 678 605 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 314 344 314
Size: 30 4178
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 535 1070 1095 535
Size: 535 25 3648
Fragments arranged by size:
3648 535 25
BseYI C'CCAG_C
Cuts at: 138 264 456 739 1633 2391 2968 3464 138
Size: 126 192 283 894 758 577 496 882
Fragments arranged by size:
894 882 758 577 496 283 192 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 958
Size: 4208
BsiEI CG_ry'CG
Cuts at: 104 1750 1796 3004 104
Size: 1646 46 1208 1308
Fragments arranged by size:
1646 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 632 674 920 1004 1062 1222 2000 2190 2982
Size: 42 246 84 58 160 778 190 792
Cuts at: 2982 632
Size: 1858
Fragments arranged by size:
1858 792 778 246 190 160 84 58
42
BsmBI CGTCTCn'nnnn_
Cuts at: 1112 1112
Size: 4208
Bsp1286I G_dGCh'C
Cuts at: 146 271 317 475 494 553 632 674 713
Size: 125 46 158 19 59 79 42 39
Cuts at: 713 920 1004 1062 1222 1833 1926 2000 2190
Size: 207 84 58 160 611 93 74 190
Cuts at: 2190 2252 2982 146
Size: 62 730 1372
Fragments arranged by size:
1372 730 611 207 190 160 158 125
93 84 79 74 62 59 58 46
42 39 19
BspEI T'CCGG_A
Cuts at: 792 3428 792
Size: 2636 1572
BspHI T'CATG_A
Cuts at: 527 3384 527
Size: 2857 1351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 775 1258 1472 1774 2155 775
Size: 483 214 302 381 2828
Fragments arranged by size:
2828 483 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2500 2554 4038 4203 50
Size: 2450 54 1484 165 55
Fragments arranged by size:
2450 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2120 2120
Size: 4208
BsrFI r'CCGG_y
Cuts at: 838 2206 2387 2596 838
Size: 1368 181 209 2450
Fragments arranged by size:
2450 1368 209 181
BssSI C'ACGA_G
Cuts at: 1003 2479 2837 1003
Size: 1476 358 2374
Fragments arranged by size:
2374 1476 358
BstAPI GCAn_nnn'nTGC
Cuts at: 453 663 1585 453
Size: 210 922 3076
Fragments arranged by size:
3076 922 210
BstBI TT'CG_AA
Cuts at: 2569 2569
Size: 4208
BstXI CCAn_nnnn'nTGG
Cuts at: 1100 1100
Size: 4208
BstYI r'GATC_y
Cuts at: 1 375 871 1103 1233 1700 1723 2058 2304
Size: 374 496 232 130 467 23 335 246
Cuts at: 2304 3316 3402 3414 3760 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 496 467 449 374 346 335 246
232 130 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 964 964
Size: 4208
BtgI C'CryG_G
Cuts at: 107 118 212 687 107
Size: 11 94 475 3628
Fragments arranged by size:
3628 475 94 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 926 2158 2339 2502 926
Size: 1232 181 163 2632
Fragments arranged by size:
2632 1232 181 163
BtsI GCAGTG_nn'
Cuts at: 1451 1451
Size: 4208
DraI TTT'AAA
Cuts at: 1207 1207
Size: 4208
DrdI GACnn_nn'nnGTC
Cuts at: 1129 1914 2772 1129
Size: 785 858 2565
Fragments arranged by size:
2565 858 785
EaeI y'GGCC_r
Cuts at: 617 827 840 1793 1967 2358 2385 3730 617
Size: 210 13 953 174 391 27 1345 1095
Fragments arranged by size:
1345 1095 953 391 210 174 27 13
EagI C'GGCC_G
Cuts at: 1793 1793
Size: 4208
EarI CTCTTCn'nnn_
Cuts at: 2231 2441 2231
Size: 210 3998
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 325 2416 2726 2872 4018 325
Size: 2091 310 146 1146 515
Fragments arranged by size:
2091 1146 515 310 146
EcoICRI GAG'CTC
Cuts at: 630 1060 1220 630
Size: 430 160 3618
Fragments arranged by size:
3618 430 160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 718 748 968 2033 2465 3212 3480 697
Size: 21 30 220 1065 432 747 268 1425
Fragments arranged by size:
1425 1065 747 432 268 220 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 544 544
Size: 4208
EcoO109I rG'GnC_Cy
Cuts at: 114 433 450 472 576 1131 1347 114
Size: 319 17 22 104 555 216 2975
Fragments arranged by size:
2975 555 319 216 104 22 17
EcoRI G'AATT_C
Cuts at: 1212 1212
Size: 4208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 4208
FspI TGC'GCA
Cuts at: 1989 1989
Size: 4208
HaeII r_GCGC'y
Cuts at: 139 776 1642 1890 2912 3891 139
Size: 637 866 248 1022 979 456
Fragments arranged by size:
1022 979 866 637 456 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 314 346 360 392 469 501 964 996 2050
Size: 32 14 32 77 32 463 32 1054
Cuts at: 2050 2080 2082 2112 314
Size: 30 2 30 2410
Fragments arranged by size:
2410 1054 463 77 32 32 32 32
30 30 14 2
HincII GTy'rAC
Cuts at: 1247 1247
Size: 4208
HindIII A'AGCT_T
Cuts at: 407 416 407
Size: 9 4199
KasI G'GCGC_C
Cuts at: 1638 1886 1638
Size: 248 3960
KpnI G_GTAC'C
Cuts at: 1228 1228
Size: 4208
MluI A'CGCG_T
Cuts at: 3641 3641
Size: 4208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2879 3063 2879
Size: 184 4024
MscI TGG'CCA
Cuts at: 1969 1969
Size: 4208
MslI CAynn'nnrTG
Cuts at: 487 2197 2324 487
Size: 1710 127 2371
Fragments arranged by size:
2371 1710 127
MspA1I CmG'CkG
Cuts at: 120 214 594 689 832 1045 1319 1633 1993
Size: 94 380 95 143 213 274 314 360
Cuts at: 1993 3006 3251 120
Size: 1013 245 1077
Fragments arranged by size:
1077 1013 380 360 314 274 245 213
143 95 94
NaeI GCC'GGC
Cuts at: 2389 2389
Size: 4208
NarI GG'CG_CC
Cuts at: 1639 1887 1639
Size: 248 3960
NcoI C'CATG_G
Cuts at: 107 107
Size: 4208
NgoMIV G'CCGG_C
Cuts at: 2387 2387
Size: 4208
NspI r_CATG'y
Cuts at: 1261 1761 2668 1261
Size: 500 907 2801
Fragments arranged by size:
2801 907 500
PasI CC'CwG_GG
Cuts at: 131 1147 131
Size: 1016 3192
PciI A'CATG_T
Cuts at: 2664 2664
Size: 4208
PmeI GTTT'AAAC
Cuts at: 1207 1207
Size: 4208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 4176
PpuMI rG'GwC_Cy
Cuts at: 114 433 1131 114
Size: 319 698 3191
Fragments arranged by size:
3191 698 319
PspOMI G'GGCC_C
Cuts at: 267 471 267
Size: 204 4004
PstI C_TGCA'G
Cuts at: 449 772 1255 1940 449
Size: 323 483 685 2717
Fragments arranged by size:
2717 685 483 323
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4208
PvuII CAG'CTG
Cuts at: 1633 1993 1633
Size: 360 3848
RsrII CG'GwC_CG
Cuts at: 2403 2403
Size: 4208
SacI G_AGCT'C
Cuts at: 632 1062 1222 632
Size: 430 160 3618
Fragments arranged by size:
3618 430 160
SacII CC_GC'GG
Cuts at: 121 215 690 121
Size: 94 475 3639
Fragments arranged by size:
3639 475 94
SalI G'TCGA_C
Cuts at: 1245 1245
Size: 4208
SanDI GG'GwC_CC
Cuts at: 114 114
Size: 4208
SapI GCTCTTCn'nnn_
Cuts at: 2231 2441 2231
Size: 210 3998
SbfI CC_TGCA'GG
Cuts at: 1255 1255
Size: 4208
ScaI AGT'ACT
Cuts at: 3725 3725
Size: 4208
SexAI A'CCwGG_T
Cuts at: 341 341
Size: 4208
SfcI C'TryA_G
Cuts at: 33 445 768 1049 1251 1936 2929 3120 33
Size: 412 323 281 202 685 993 191 1121
Fragments arranged by size:
1121 993 685 412 323 281 202 191
SfoI GGC'GCC
Cuts at: 1640 1888 1640
Size: 248 3960
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
SmaI CCC'GGG
Cuts at: 233 1230 233
Size: 997 3211
SmlI C'TyrA_G
Cuts at: 541 1363 2278 2770 3032 541
Size: 822 915 492 262 1717
Fragments arranged by size:
1717 915 822 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4208
SphI G_CATG'C
Cuts at: 1261 1761 1261
Size: 500 3708
StyI C'CwwG_G
Cuts at: 107 1099 1342 1696 107
Size: 992 243 354 2619
Fragments arranged by size:
2619 992 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 240 1791 1924 2383 2592 240
Size: 1551 133 459 209 1856
Fragments arranged by size:
1856 1551 459 209 133
TatI w'GTAC_w
Cuts at: 3723 3723
Size: 4208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1068 1100 3725 3757 1068
Size: 32 2625 32 1519
Fragments arranged by size:
2625 1519 32 32
Tth111I GACn'n_nGTC
Cuts at: 2005 2005
Size: 4208
XbaI T'CTAG_A
Cuts at: 1239 1239
Size: 4208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1703 1703
Size: 4208
XmaI C'CCGG_G
Cuts at: 231 1228 231
Size: 997 3211
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI