Restriction map for
pF1KB6516
(Circular) (Six-base) MAPSORT of: pF1KB6516.seq Check: 2764 from: 1 to: 4268
pF1KB6516 4268 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 691 691
Size: 4268
AccI GT'mk_AC
Cuts at: 1306 1306
Size: 4268
Acc65I G'GTAC_C
Cuts at: 763 1284 763
Size: 521 3747
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 780 786 804 851 966 2093 2525 3272
Size: 289 6 18 47 115 1127 432 747
Cuts at: 3272 3540 491
Size: 268 1219
Fragments arranged by size:
1219 1127 747 432 289 268 115 47
18 6
AflIII A'CryG_T
Cuts at: 1620 2724 3701 1620
Size: 1104 977 2187
Fragments arranged by size:
2187 1104 977
AgeI A'CCGG_T
Cuts at: 2656 2656
Size: 4268
AlwNI CAG_nnn'CTG
Cuts at: 1573 3140 1573
Size: 1567 2701
ApaI G_GGCC'C
Cuts at: 713 713
Size: 4268
ApaLI G'TGCA_C
Cuts at: 3038 3038
Size: 4268
ApoI r'AATT_y
Cuts at: 1272 3621 3877 1272
Size: 2349 256 1663
Fragments arranged by size:
2349 1663 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 1288 1288
Size: 4268
BamHI G'GATC_C
Cuts at: 1293 1293
Size: 4268
BanI G'GyrC_C
Cuts at: 763 1284 1698 1946 1981 763
Size: 521 414 248 35 3050
Fragments arranged by size:
3050 521 414 248 35
BanII G_rGCy'C
Cuts at: 503 713 1282 2312 503
Size: 210 569 1030 2459
Fragments arranged by size:
2459 1030 569 210
BbeI G_GCGC'C
Cuts at: 1702 1950 1702
Size: 248 4020
BbsI GAAGACnn'nnnn_
Cuts at: 385 385
Size: 4268
BciVI GTATCCnnnnn_n'
Cuts at: 2166 2927 4268 2166
Size: 761 1341 2166
Fragments arranged by size:
2166 1341 761
BclI T'GATC_A
Cuts at: 1788 1788
Size: 4268
BglII A'GATC_T
Cuts at: 536 1783 3820 536
Size: 1247 2037 984
Fragments arranged by size:
2037 1247 984
BlpI GC'TnA_GC
Cuts at: 1380 1380
Size: 4268
Bme1580I G_kGCm'C
Cuts at: 713 1893 1986 3042 713
Size: 1180 93 1056 1939
Fragments arranged by size:
1939 1180 1056 93
BmgBI CAC'GTC
Cuts at: 443 443
Size: 4268
BmrI ACTGGGnnnn_n'
Cuts at: 495 708 857 1480 1561 1896 4040 495
Size: 213 149 623 81 335 2144 723
Fragments arranged by size:
2144 723 623 335 213 149 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 878 394
Size: 484 3784
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 2323 2815 3113 1444
Size: 879 492 298 2599
Fragments arranged by size:
2599 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3972 3972
Size: 4268
BsaAI yAC'GTr
Cuts at: 1621 1621
Size: 4268
BsaBI GATnn'nnATC
Cuts at: 6 415 541 1782 3819 6
Size: 409 126 1241 2037 455
Fragments arranged by size:
2037 1241 455 409 126
BsaHI Gr'CG_yC
Cuts at: 581 1699 1947 2649 4145 581
Size: 1118 248 702 1496 704
Fragments arranged by size:
1496 1118 704 702 248
BsaWI w'CCGG_w
Cuts at: 419 1457 1978 2656 2930 3077 3488 419
Size: 1038 521 678 274 147 411 1199
Fragments arranged by size:
1199 1038 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 644 674 644
Size: 30 4238
BseYI C'CCAG_C
Cuts at: 759 1693 2451 3028 3524 759
Size: 934 758 577 496 1503
Fragments arranged by size:
1503 934 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1810 1856 3064 104
Size: 1706 46 1208 1308
Fragments arranged by size:
1706 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 175 1282 2060 2250 3042 175
Size: 1107 778 190 792 1401
Fragments arranged by size:
1401 1107 792 778 190
BsmI GAATG_Cn'
Cuts at: 1177 1177
Size: 4268
Bsp1286I G_dGCh'C
Cuts at: 175 503 713 1282 1893 1986 2060 2250 2312
Size: 328 210 569 611 93 74 190 62
Cuts at: 2312 3042 175
Size: 730 1401
Fragments arranged by size:
1401 730 611 569 328 210 190 93
74 62
BspEI T'CCGG_A
Cuts at: 419 3488 419
Size: 3069 1199
BspHI T'CATG_A
Cuts at: 953 3444 953
Size: 2491 1777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 691 1318 1532 1834 2215 691
Size: 627 214 302 381 2744
Fragments arranged by size:
2744 627 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2560 2614 4098 4263 50
Size: 2510 54 1484 165 55
Fragments arranged by size:
2510 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2180 2180
Size: 4268
BsrFI r'CCGG_y
Cuts at: 2266 2447 2656 2266
Size: 181 209 3878
Fragments arranged by size:
3878 209 181
BssSI C'ACGA_G
Cuts at: 2539 2897 2539
Size: 358 3910
BstAPI GCAn_nnn'nTGC
Cuts at: 1645 1645
Size: 4268
BstBI TT'CG_AA
Cuts at: 2629 2629
Size: 4268
BstYI r'GATC_y
Cuts at: 1 536 1293 1760 1783 2118 2364 3376 3462
Size: 535 757 467 23 335 246 1012 86
Cuts at: 3462 3474 3820 1
Size: 12 346 449
Fragments arranged by size:
1012 757 535 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 157 648 767 107
Size: 50 491 119 3608
Fragments arranged by size:
3608 491 119 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2218 2399 2562 2218
Size: 181 163 3924
Fragments arranged by size:
3924 181 163
BtsI GCAGTG_nn'
Cuts at: 648 1511 648
Size: 863 3405
DraI TTT'AAA
Cuts at: 1267 1267
Size: 4268
DraIII CAC_nnn'GTG
Cuts at: 814 814
Size: 4268
DrdI GACnn_nn'nnGTC
Cuts at: 1974 2832 1974
Size: 858 3410
EaeI y'GGCC_r
Cuts at: 645 974 1853 2027 2418 2445 3790 645
Size: 329 879 174 391 27 1345 1123
Fragments arranged by size:
1345 1123 879 391 329 174 27
EagI C'GGCC_G
Cuts at: 1853 1853
Size: 4268
EarI CTCTTCn'nnn_
Cuts at: 1153 2291 2501 1153
Size: 1138 210 2920
Fragments arranged by size:
2920 1138 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 373 2476 2786 2932 4078 373
Size: 2103 310 146 1146 563
Fragments arranged by size:
2103 1146 563 310 146
EcoICRI GAG'CTC
Cuts at: 1280 1280
Size: 4268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 491 780 786 804 851 878 966 2093
Size: 97 289 6 18 47 27 88 1127
Cuts at: 2093 2525 3272 3540 394
Size: 432 747 268 1122
Fragments arranged by size:
1127 1122 747 432 289 268 97 88
47 27 18 6
EcoO109I rG'GnC_Cy
Cuts at: 710 750 1407 710
Size: 40 657 3571
Fragments arranged by size:
3571 657 40
EcoRI G'AATT_C
Cuts at: 1272 1272
Size: 4268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 4268
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 1702 1950 2972 3951 1702
Size: 248 1022 979 2019
Fragments arranged by size:
2019 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 916 948 2110 2140 2142 2172 916
Size: 32 1162 30 2 30 3012
Fragments arranged by size:
3012 1162 32 30 30 2
HincII GTy'rAC
Cuts at: 1307 1307
Size: 4268
KasI G'GCGC_C
Cuts at: 1698 1946 1698
Size: 248 4020
KpnI G_GTAC'C
Cuts at: 767 1288 767
Size: 521 3747
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2939 3123 2939
Size: 184 4084
MscI TGG'CCA
Cuts at: 647 976 2029 647
Size: 329 1053 2886
Fragments arranged by size:
2886 1053 329
MslI CAynn'nnrTG
Cuts at: 162 1156 2257 2384 162
Size: 994 1101 127 2046
Fragments arranged by size:
2046 1101 994 127
MspA1I CmG'CkG
Cuts at: 824 1379 1693 2053 3066 3311 824
Size: 555 314 360 1013 245 1781
Fragments arranged by size:
1781 1013 555 360 314 245
NaeI GCC'GGC
Cuts at: 2449 2449
Size: 4268
NarI GG'CG_CC
Cuts at: 1699 1947 1699
Size: 248 4020
NcoI C'CATG_G
Cuts at: 107 157 648 767 107
Size: 50 491 119 3608
Fragments arranged by size:
3608 491 119 50
NdeI CA'TA_TG
Cuts at: 1260 1260
Size: 4268
NgoMIV G'CCGG_C
Cuts at: 2447 2447
Size: 4268
NspI r_CATG'y
Cuts at: 1001 1321 1821 2728 1001
Size: 320 500 907 2541
Fragments arranged by size:
2541 907 500 320
PciI A'CATG_T
Cuts at: 2724 2724
Size: 4268
PflMI CCAn_nnn'nTGG
Cuts at: 293 972 293
Size: 679 3589
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 4236
PsiI TTA'TAA
Cuts at: 1139 1139
Size: 4268
PspOMI G'GGCC_C
Cuts at: 709 709
Size: 4268
PstI C_TGCA'G
Cuts at: 118 1315 2000 118
Size: 1197 685 2386
Fragments arranged by size:
2386 1197 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 824 1693 2053 824
Size: 869 360 3039
Fragments arranged by size:
3039 869 360
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 1282 1282
Size: 4268
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 2291 2501 2291
Size: 210 4058
SbfI CC_TGCA'GG
Cuts at: 1315 1315
Size: 4268
ScaI AGT'ACT
Cuts at: 3785 3785
Size: 4268
SfcI C'TryA_G
Cuts at: 33 114 960 1311 1996 2989 3180 33
Size: 81 846 351 685 993 191 1121
Fragments arranged by size:
1121 993 846 685 351 191 81
SfoI GGC'GCC
Cuts at: 1700 1948 1700
Size: 248 4020
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 1290 1290
Size: 4268
SmlI C'TyrA_G
Cuts at: 1423 2338 2830 3092 1423
Size: 915 492 262 2599
Fragments arranged by size:
2599 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4268
SphI G_CATG'C
Cuts at: 1321 1821 1321
Size: 500 3768
StyI C'CwwG_G
Cuts at: 107 157 648 767 1402 1756 107
Size: 50 491 119 635 354 2619
Fragments arranged by size:
2619 635 491 354 119 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 190 435 1851 1984 2443 2652 190
Size: 245 1416 133 459 209 1806
Fragments arranged by size:
1806 1416 459 245 209 133
TatI w'GTAC_w
Cuts at: 775 3783 775
Size: 3008 1260
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4236
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 1299 1299
Size: 4268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 881 1763 881
Size: 882 3386
XmaI C'CCGG_G
Cuts at: 1288 1288
Size: 4268
XmnI GAAnn'nnTTC
Cuts at: 196 196
Size: 4268
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI