Restriction map for
pF1KB6501
(Circular) (Six-base) MAPSORT of: pF1KB6501.seq Check: 6230 from: 1 to: 4187
pF1KB6501 4187 bp
With 182 enzymes: SgfI *
March 30, 2011 17:32 ..
AatII G_ACGT'C
Cuts at: 362 362
Size: 4187
AccI GT'mk_AC
Cuts at: 614 701 1225 614
Size: 87 524 3576
Fragments arranged by size:
3576 524 87
Acc65I G'GTAC_C
Cuts at: 1203 1203
Size: 4187
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 495 1075 2012 2444 3191 3459 430
Size: 65 580 937 432 747 268 1158
Fragments arranged by size:
1158 937 747 580 432 268 65
AfeI AGC'GCT
Cuts at: 466 466
Size: 4187
AflIII A'CryG_T
Cuts at: 384 1539 2643 3620 384
Size: 1155 1104 977 951
Fragments arranged by size:
1155 1104 977 951
AgeI A'CCGG_T
Cuts at: 2575 2575
Size: 4187
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 667 699 667
Size: 32 4155
AlwNI CAG_nnn'CTG
Cuts at: 1492 3059 1492
Size: 1567 2620
ApaI G_GGCC'C
Cuts at: 795 795
Size: 4187
ApaLI G'TGCA_C
Cuts at: 295 2957 295
Size: 2662 1525
ApoI r'AATT_y
Cuts at: 872 1176 1191 3540 3796 872
Size: 304 15 2349 256 1263
Fragments arranged by size:
2349 1263 304 256 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 118 730 1207 118
Size: 612 477 3098
Fragments arranged by size:
3098 612 477
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 718 751 718
Size: 33 4154
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 114 186 1203 1617 1865 1900 114
Size: 72 1017 414 248 35 2401
Fragments arranged by size:
2401 1017 414 248 72 35
BanII G_rGCy'C
Cuts at: 509 557 795 1201 2231 509
Size: 48 238 406 1030 2465
Fragments arranged by size:
2465 1030 406 238 48
BbeI G_GCGC'C
Cuts at: 118 190 1621 1869 118
Size: 72 1431 248 2436
Fragments arranged by size:
2436 1431 248 72
BbsI GAAGACnn'nnnn_
Cuts at: 319 319
Size: 4187
BciVI GTATCCnnnnn_n'
Cuts at: 2085 2846 4187 2085
Size: 761 1341 2085
Fragments arranged by size:
2085 1341 761
BclI T'GATC_A
Cuts at: 1707 1707
Size: 4187
BglI GCCn_nnn'nGGC
Cuts at: 112 133 112
Size: 21 4166
BglII A'GATC_T
Cuts at: 1702 3739 1702
Size: 2037 2150
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 277 299 795 1073 1812 1905 2961 277
Size: 22 496 278 739 93 1056 1503
Fragments arranged by size:
1503 1056 739 496 278 93 22
BmgBI CAC'GTC
Cuts at: 452 452
Size: 4187
BmrI ACTGGGnnnn_n'
Cuts at: 287 1038 1137 1399 1480 1815 3959 287
Size: 751 99 262 81 335 2144 515
Fragments arranged by size:
2144 751 515 335 262 99 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 168 2554 168
Size: 2386 1801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 525 525
Size: 4187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1363 2242 2734 3032 322
Size: 1041 879 492 298 1477
Fragments arranged by size:
1477 1041 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 166 3891 166
Size: 3725 462
BsaAI yAC'GTr
Cuts at: 387 492 1540 387
Size: 105 1048 3034
Fragments arranged by size:
3034 1048 105
BsaBI GATnn'nnATC
Cuts at: 6 1701 3738 6
Size: 1695 2037 455
Fragments arranged by size:
2037 1695 455
BsaHI Gr'CG_yC
Cuts at: 115 187 359 1618 1866 2568 4064 115
Size: 72 172 1259 248 702 1496 238
Fragments arranged by size:
1496 1259 702 248 238 172 72
BsaWI w'CCGG_w
Cuts at: 1376 1897 2575 2849 2996 3407 1376
Size: 521 678 274 147 411 2156
Fragments arranged by size:
2156 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 583 613 583
Size: 30 4157
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 184
Size: 4187
BseYI C'CCAG_C
Cuts at: 202 1612 2370 2947 3443 202
Size: 1410 758 577 496 946
Fragments arranged by size:
1410 946 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 580
Size: 4187
BsiEI CG_ry'CG
Cuts at: 104 137 1729 1775 2983 104
Size: 33 1592 46 1208 1308
Fragments arranged by size:
1592 1308 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 299 509 1201 1979 2169 2961 299
Size: 210 692 778 190 792 1525
Fragments arranged by size:
1525 792 778 692 210 190
BsmI GAATG_Cn'
Cuts at: 350 350
Size: 4187
Bsp1286I G_dGCh'C
Cuts at: 277 299 509 557 795 1073 1201 1812 1905
Size: 22 210 48 238 278 128 611 93
Cuts at: 1905 1979 2169 2231 2961 277
Size: 74 190 62 730 1503
Fragments arranged by size:
1503 730 611 278 238 210 190 128
93 74 62 48 22
BspEI T'CCGG_A
Cuts at: 3407 3407
Size: 4187
BspHI T'CATG_A
Cuts at: 3363 3363
Size: 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 527 602 1237 1451 1753 2134 527
Size: 75 635 214 302 381 2580
Fragments arranged by size:
2580 635 381 302 214 75
BsrBI CCG'CTC
Cuts at: 50 1019 2479 2533 4017 4182 50
Size: 969 1460 54 1484 165 55
Fragments arranged by size:
1484 1460 969 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2099
Size: 4187
BsrFI r'CCGG_y
Cuts at: 2185 2366 2575 2185
Size: 181 209 3797
Fragments arranged by size:
3797 209 181
BssSI C'ACGA_G
Cuts at: 943 2458 2816 943
Size: 1515 358 2314
Fragments arranged by size:
2314 1515 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1564 1564
Size: 4187
BstBI TT'CG_AA
Cuts at: 1106 2548 1106
Size: 1442 2745
BstXI CCAn_nnnn'nTGG
Cuts at: 696 906 696
Size: 210 3977
BstYI r'GATC_y
Cuts at: 1 1212 1679 1702 2037 2283 3295 3381 3393
Size: 1211 467 23 335 246 1012 86 12
Cuts at: 3393 3739 1
Size: 346 449
Fragments arranged by size:
1211 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 209 209
Size: 4187
BtgI C'CryG_G
Cuts at: 107 137 269 548 768 779 1022 107
Size: 30 132 279 220 11 243 3272
Fragments arranged by size:
3272 279 243 220 132 30 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2137 2318 2481 2137
Size: 181 163 3843
Fragments arranged by size:
3843 181 163
BtsI GCAGTG_nn'
Cuts at: 1430 1430
Size: 4187
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DraIII CAC_nnn'GTG
Cuts at: 303 303
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 2751 1893
Size: 858 3329
EaeI y'GGCC_r
Cuts at: 134 263 657 1772 1946 2337 2364 3709 134
Size: 129 394 1115 174 391 27 1345 612
Fragments arranged by size:
1345 1115 612 394 391 174 129 27
EagI C'GGCC_G
Cuts at: 134 1772 134
Size: 1638 2549
EarI CTCTTCn'nnn_
Cuts at: 514 562 2210 2420 514
Size: 48 1648 210 2281
Fragments arranged by size:
2281 1648 210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 157 181 355 2395 2705 2851 3997 157
Size: 24 174 2040 310 146 1146 347
Fragments arranged by size:
2040 1146 347 310 174 146 24
EcoICRI GAG'CTC
Cuts at: 507 1199 507
Size: 692 3495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 495 525 1075 2012 2444 3191 3459 430
Size: 65 30 550 937 432 747 268 1158
Fragments arranged by size:
1158 937 747 550 432 268 65 30
EcoNI CCTnn'n_nnAGG
Cuts at: 304 373 304
Size: 69 4118
EcoO109I rG'GnC_Cy
Cuts at: 792 1326 792
Size: 534 3653
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 345 2206 345
Size: 1861 2326
FspI TGC'GCA
Cuts at: 523 1968 523
Size: 1445 2742
HaeII r_GCGC'y
Cuts at: 118 190 217 468 537 1621 1869 2891 3870
Size: 72 27 251 69 1084 248 1022 979
Cuts at: 3870 118
Size: 435
Fragments arranged by size:
1084 1022 979 435 251 248 72 69
27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 2029 2059 2061 2091 202
Size: 32 1795 30 2 30 2298
Fragments arranged by size:
2298 1795 32 30 30 2
HincII GTy'rAC
Cuts at: 951 1226 951
Size: 275 3912
KasI G'GCGC_C
Cuts at: 114 186 1617 1865 114
Size: 72 1431 248 2436
Fragments arranged by size:
2436 1431 248 72
KpnI G_GTAC'C
Cuts at: 1207 1207
Size: 4187
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1124 2858 3042 1124
Size: 1734 184 2269
Fragments arranged by size:
2269 1734 184
MscI TGG'CCA
Cuts at: 265 659 1948 265
Size: 394 1289 2504
Fragments arranged by size:
2504 1289 394
MslI CAynn'nnrTG
Cuts at: 481 2176 2303 481
Size: 1695 127 2365
Fragments arranged by size:
2365 1695 127
MspA1I CmG'CkG
Cuts at: 139 1298 1612 1972 2985 3230 139
Size: 1159 314 360 1013 245 1096
Fragments arranged by size:
1159 1096 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2368 2368
Size: 4187
NarI GG'CG_CC
Cuts at: 115 187 1618 1866 115
Size: 72 1431 248 2436
Fragments arranged by size:
2436 1431 248 72
NcoI C'CATG_G
Cuts at: 107 107
Size: 4187
NgoMIV G'CCGG_C
Cuts at: 2366 2366
Size: 4187
NotI GC'GGCC_GC
Cuts at: 134 134
Size: 4187
NspI r_CATG'y
Cuts at: 480 1240 1740 2647 480
Size: 760 500 907 2020
Fragments arranged by size:
2020 907 760 500
PciI A'CATG_T
Cuts at: 2643 2643
Size: 4187
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 4155
PspOMI G'GGCC_C
Cuts at: 791 791
Size: 4187
PstI C_TGCA'G
Cuts at: 248 570 1234 1919 248
Size: 322 664 685 2516
Fragments arranged by size:
2516 685 664 322
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 1612 1972 1612
Size: 360 3827
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 509 1201 509
Size: 692 3495
SacII CC_GC'GG
Cuts at: 140 140
Size: 4187
SalI G'TCGA_C
Cuts at: 1224 1224
Size: 4187
SapI GCTCTTCn'nnn_
Cuts at: 514 562 2210 2420 514
Size: 48 1648 210 2281
Fragments arranged by size:
2281 1648 210 48
SbfI CC_TGCA'GG
Cuts at: 248 1234 248
Size: 986 3201
ScaI AGT'ACT
Cuts at: 3704 3704
Size: 4187
SexAI A'CCwGG_T
Cuts at: 299 299
Size: 4187
SfcI C'TryA_G
Cuts at: 33 244 566 615 1230 1915 2908 3099 33
Size: 211 322 49 615 685 993 191 1121
Fragments arranged by size:
1121 993 685 615 322 211 191 49
SfiI GGCCn_nnn'nGGCC
Cuts at: 133 133
Size: 4187
SfoI GGC'GCC
Cuts at: 116 188 1619 1867 116
Size: 72 1431 248 2436
Fragments arranged by size:
2436 1431 248 72
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 120 1209 120
Size: 1089 3098
SmlI C'TyrA_G
Cuts at: 337 1342 2257 2749 3011 337
Size: 1005 915 492 262 1513
Fragments arranged by size:
1513 1005 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4187
SphI G_CATG'C
Cuts at: 1240 1740 1240
Size: 500 3687
SrfI GCCC'GGGC
Cuts at: 120 120
Size: 4187
StyI C'CwwG_G
Cuts at: 107 1321 1675 107
Size: 1214 354 2619
Fragments arranged by size:
2619 1214 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1770 1903 2362 2571 1770
Size: 133 459 209 3386
Fragments arranged by size:
3386 459 209 133
TatI w'GTAC_w
Cuts at: 3702 3702
Size: 4187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 705 737 764 796 3704 3736 705
Size: 32 27 32 2908 32 1156
Fragments arranged by size:
2908 1156 32 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 1984 1984
Size: 4187
XbaI T'CTAG_A
Cuts at: 1218 1218
Size: 4187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1682 1682
Size: 4187
XmaI C'CCGG_G
Cuts at: 118 1207 118
Size: 1089 3098
XmnI GAAnn'nnTTC
Cuts at: 752 1037 752
Size: 285 3902
ZraI GAC'GTC
Cuts at: 360 360
Size: 4187
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AscI AvrII BbvCI
BcgI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SgrAI SnaBI SspI StuI SwaI XhoI