Restriction map for
pF1KB6496
(Circular) (Six-base) MAPSORT of: pF1KB6496.seq Check: 7415 from: 1 to: 4187
pF1KB6496 4187 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1160 1225 1160
Size: 65 4122
Acc65I G'GTAC_C
Cuts at: 1203 1203
Size: 4187
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 2444 3191 3459 2012
Size: 432 747 268 2740
Fragments arranged by size:
2740 747 432 268
AflIII A'CryG_T
Cuts at: 1539 2643 3620 1539
Size: 1104 977 2106
Fragments arranged by size:
2106 1104 977
AgeI A'CCGG_T
Cuts at: 2575 2575
Size: 4187
AleI CACnn'nnGTG
Cuts at: 889 889
Size: 4187
AlwNI CAG_nnn'CTG
Cuts at: 123 315 1134 1492 3059 123
Size: 192 819 358 1567 1251
Fragments arranged by size:
1567 1251 819 358 192
ApaI G_GGCC'C
Cuts at: 652 652
Size: 4187
ApaLI G'TGCA_C
Cuts at: 2957 2957
Size: 4187
ApoI r'AATT_y
Cuts at: 304 552 1191 3540 3796 304
Size: 248 639 2349 256 695
Fragments arranged by size:
2349 695 639 256 248
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 971 1207 971
Size: 236 3951
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 201 1203 1617 1865 1900 201
Size: 1002 414 248 35 2488
Fragments arranged by size:
2488 1002 414 248 35
BanII G_rGCy'C
Cuts at: 409 652 1025 1057 1201 2231 409
Size: 243 373 32 144 1030 2365
Fragments arranged by size:
2365 1030 373 243 144 32
BbeI G_GCGC'C
Cuts at: 1621 1869 1621
Size: 248 3939
BciVI GTATCCnnnnn_n'
Cuts at: 2085 2846 4187 2085
Size: 761 1341 2085
Fragments arranged by size:
2085 1341 761
BclI T'GATC_A
Cuts at: 1707 1707
Size: 4187
BglII A'GATC_T
Cuts at: 191 516 1702 3739 191
Size: 325 1186 2037 639
Fragments arranged by size:
2037 1186 639 325
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 652 1812 1905 2961 652
Size: 1160 93 1056 1878
Fragments arranged by size:
1878 1160 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1399 1480 1815 3959 1399
Size: 81 335 2144 1627
Fragments arranged by size:
2144 1627 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 836 836
Size: 4187
Bpu10I CC'TnA_GC
Cuts at: 124 124
Size: 4187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1363 2242 2734 3032 1363
Size: 879 492 298 2518
Fragments arranged by size:
2518 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3891 3891
Size: 4187
BsaAI yAC'GTr
Cuts at: 801 1540 801
Size: 739 3448
BsaBI GATnn'nnATC
Cuts at: 6 398 1701 3738 6
Size: 392 1303 2037 455
Fragments arranged by size:
2037 1303 455 392
BsaHI Gr'CG_yC
Cuts at: 1618 1866 2568 4064 1618
Size: 248 702 1496 1741
Fragments arranged by size:
1741 1496 702 248
BsaWI w'CCGG_w
Cuts at: 272 1376 1897 2575 2849 2996 3407 272
Size: 1104 521 678 274 147 411 1052
Fragments arranged by size:
1104 1052 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 884 884
Size: 4187
BseYI C'CCAG_C
Cuts at: 641 645 1612 2370 2947 3443 641
Size: 4 967 758 577 496 1385
Fragments arranged by size:
1385 967 758 577 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 1171
Size: 4187
BsiEI CG_ry'CG
Cuts at: 104 273 1729 1775 2983 104
Size: 169 1456 46 1208 1308
Fragments arranged by size:
1456 1308 1208 169 46
BsiHKAI G_wGCw'C
Cuts at: 130 409 1201 1979 2169 2961 130
Size: 279 792 778 190 792 1356
Fragments arranged by size:
1356 792 792 778 279 190
BsmI GAATG_Cn'
Cuts at: 697 697
Size: 4187
BsmBI CGTCTCn'nnnn_
Cuts at: 752 752
Size: 4187
Bsp1286I G_dGCh'C
Cuts at: 130 409 652 1025 1057 1201 1812 1905 1979
Size: 279 243 373 32 144 611 93 74
Cuts at: 1979 2169 2231 2961 130
Size: 190 62 730 1356
Fragments arranged by size:
1356 730 611 373 279 243 190 144
93 74 62 32
BspEI T'CCGG_A
Cuts at: 3407 3407
Size: 4187
BspHI T'CATG_A
Cuts at: 3363 3363
Size: 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1237 1451 1753 2134 1237
Size: 214 302 381 3290
Fragments arranged by size:
3290 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2479 2533 4017 4182 50
Size: 2429 54 1484 165 55
Fragments arranged by size:
2429 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2099
Size: 4187
BsrFI r'CCGG_y
Cuts at: 2185 2366 2575 2185
Size: 181 209 3797
Fragments arranged by size:
3797 209 181
BsrGI T'GTAC_A
Cuts at: 881 881
Size: 4187
BssSI C'ACGA_G
Cuts at: 2458 2816 2458
Size: 358 3829
BstAPI GCAn_nnn'nTGC
Cuts at: 134 630 1564 134
Size: 496 934 2757
Fragments arranged by size:
2757 934 496
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstXI CCAn_nnnn'nTGG
Cuts at: 645 645
Size: 4187
BstYI r'GATC_y
Cuts at: 1 191 312 516 1212 1679 1702 2037 2283
Size: 190 121 204 696 467 23 335 246
Cuts at: 2283 3295 3381 3393 3739 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 696 467 449 346 335 246 204
190 121 86 23 12
BstZ17I GTA'TAC
Cuts at: 1161 1161
Size: 4187
BtgI C'CryG_G
Cuts at: 107 411 107
Size: 304 3883
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2137 2318 2481 2137
Size: 181 163 3843
Fragments arranged by size:
3843 181 163
BtsI GCAGTG_nn'
Cuts at: 1430 1430
Size: 4187
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 2751 1893
Size: 858 3329
EaeI y'GGCC_r
Cuts at: 1772 1946 2337 2364 3709 1772
Size: 174 391 27 1345 2250
Fragments arranged by size:
2250 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1772 1772
Size: 4187
EarI CTCTTCn'nnn_
Cuts at: 2210 2420 2210
Size: 210 3977
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2395 2705 2851 3997 2395
Size: 310 146 1146 2585
Fragments arranged by size:
2585 1146 310 146
EcoICRI GAG'CTC
Cuts at: 407 1199 407
Size: 792 3395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 836 2012 2444 3191 3459 836
Size: 1176 432 747 268 1564
Fragments arranged by size:
1564 1176 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 649 1326 649
Size: 677 3510
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 4187
FspI TGC'GCA
Cuts at: 1968 1968
Size: 4187
HaeII r_GCGC'y
Cuts at: 660 1621 1869 2891 3870 660
Size: 961 248 1022 979 977
Fragments arranged by size:
1022 979 977 961 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 354 1106 1138 2029 2059 2061 2091 322
Size: 32 752 32 891 30 2 30 2418
Fragments arranged by size:
2418 891 752 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1100 1226 1100
Size: 126 4061
HpaI GTT'AAC
Cuts at: 1100 1100
Size: 4187
KasI G'GCGC_C
Cuts at: 1617 1865 1617
Size: 248 3939
KpnI G_GTAC'C
Cuts at: 1207 1207
Size: 4187
MfeI C'AATT_G
Cuts at: 615 615
Size: 4187
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2858 3042 2858
Size: 184 4003
MscI TGG'CCA
Cuts at: 1948 1948
Size: 4187
MslI CAynn'nnrTG
Cuts at: 803 889 947 2176 2303 803
Size: 86 58 1229 127 2687
Fragments arranged by size:
2687 1229 127 86 58
MspA1I CmG'CkG
Cuts at: 645 1298 1612 1972 2985 3230 645
Size: 653 314 360 1013 245 1602
Fragments arranged by size:
1602 1013 653 360 314 245
NaeI GCC'GGC
Cuts at: 2368 2368
Size: 4187
NarI GG'CG_CC
Cuts at: 1618 1866 1618
Size: 248 3939
NcoI C'CATG_G
Cuts at: 107 411 107
Size: 304 3883
NgoMIV G'CCGG_C
Cuts at: 2366 2366
Size: 4187
NspI r_CATG'y
Cuts at: 1240 1740 2647 1240
Size: 500 907 2780
Fragments arranged by size:
2780 907 500
PasI CC'CwG_GG
Cuts at: 652 652
Size: 4187
PciI A'CATG_T
Cuts at: 2643 2643
Size: 4187
PflMI CCAn_nnn'nTGG
Cuts at: 315 678 315
Size: 363 3824
PfoI T'CCnGG_A
Cuts at: 308 308
Size: 4187
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PmlI CAC'GTG
Cuts at: 801 801
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 4155
PspOMI G'GGCC_C
Cuts at: 648 648
Size: 4187
PstI C_TGCA'G
Cuts at: 260 1234 1919 260
Size: 974 685 2528
Fragments arranged by size:
2528 974 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 645 1612 1972 645
Size: 967 360 2860
Fragments arranged by size:
2860 967 360
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 409 1201 409
Size: 792 3395
SalI G'TCGA_C
Cuts at: 1224 1224
Size: 4187
SapI GCTCTTCn'nnn_
Cuts at: 2210 2420 2210
Size: 210 3977
SbfI CC_TGCA'GG
Cuts at: 1234 1234
Size: 4187
ScaI AGT'ACT
Cuts at: 3704 3704
Size: 4187
SfcI C'TryA_G
Cuts at: 33 240 256 1230 1915 2908 3099 33
Size: 207 16 974 685 993 191 1121
Fragments arranged by size:
1121 993 974 685 207 191 16
SfoI GGC'GCC
Cuts at: 1619 1867 1619
Size: 248 3939
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 973 1209 973
Size: 236 3951
SmlI C'TyrA_G
Cuts at: 1342 2257 2749 3011 1342
Size: 915 492 262 2518
Fragments arranged by size:
2518 915 492 262
SpeI A'CTAG_T
Cuts at: 67 708 67
Size: 641 3546
SphI G_CATG'C
Cuts at: 1240 1740 1240
Size: 500 3687
SspI AAT'ATT
Cuts at: 489 857 489
Size: 368 3819
StuI AGG'CCT
Cuts at: 238 238
Size: 4187
StyI C'CwwG_G
Cuts at: 107 411 1321 1675 107
Size: 304 910 354 2619
Fragments arranged by size:
2619 910 354 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 307 655 866 1770 1903 2362 2571 307
Size: 348 211 904 133 459 209 1923
Fragments arranged by size:
1923 904 459 348 211 209 133
TatI w'GTAC_w
Cuts at: 881 3702 881
Size: 2821 1366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 542 574 3704 3736 542
Size: 32 3130 32 993
Fragments arranged by size:
3130 993 32 32
Tth111I GACn'n_nGTC
Cuts at: 1984 1984
Size: 4187
XbaI T'CTAG_A
Cuts at: 519 1218 519
Size: 699 3488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 649 1682 649
Size: 1033 3154
XmaI C'CCGG_G
Cuts at: 971 1207 971
Size: 236 3951
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BssHII BstEII Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII NdeI NheI
NotI NruI NsiI PacI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI