Restriction map for
pF1KB6492
(Circular) (Six-base) MAPSORT of: pF1KB6492.seq Check: 1947 from: 1 to: 4184
pF1KB6492 4184 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 492 492
Size: 4184
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 799 2009 2441 3188 3456 376
Size: 423 1210 432 747 268 1104
Fragments arranged by size:
1210 1104 747 432 423 268
AflIII A'CryG_T
Cuts at: 1536 2640 3617 1536
Size: 1104 977 2103
Fragments arranged by size:
2103 1104 977
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 772 772
Size: 4184
AlwNI CAG_nnn'CTG
Cuts at: 312 810 1140 1489 3056 312
Size: 498 330 349 1567 1440
Fragments arranged by size:
1567 1440 498 349 330
ApaI G_GGCC'C
Cuts at: 182 182
Size: 4184
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 595 1188 3537 3793 595
Size: 593 2349 256 986
Fragments arranged by size:
2349 986 593 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 175 1204 175
Size: 1029 3155
BamHI G'GATC_C
Cuts at: 1209 1209
Size: 4184
BanI G'GyrC_C
Cuts at: 111 526 1200 1614 1862 1897 111
Size: 415 674 414 248 35 2398
Fragments arranged by size:
2398 674 415 414 248 35
BanII G_rGCy'C
Cuts at: 146 182 344 872 950 1164 1198 2228 146
Size: 36 162 528 78 214 34 1030 2102
Fragments arranged by size:
2102 1030 528 214 162 78 36 34
BbeI G_GCGC'C
Cuts at: 115 1618 1866 115
Size: 1503 248 2433
Fragments arranged by size:
2433 1503 248
BbsI GAAGACnn'nnnn_
Cuts at: 542 589 542
Size: 47 4137
BbvCI CC'TCA_GC
Cuts at: 1021 1021
Size: 4184
BciVI GTATCCnnnnn_n'
Cuts at: 2082 2843 4184 2082
Size: 761 1341 2082
Fragments arranged by size:
2082 1341 761
BclI T'GATC_A
Cuts at: 1704 1704
Size: 4184
BglI GCCn_nnn'nGGC
Cuts at: 203 839 203
Size: 636 3548
BglII A'GATC_T
Cuts at: 1699 3736 1699
Size: 2037 2147
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 182 1809 1902 2958 182
Size: 1627 93 1056 1408
Fragments arranged by size:
1627 1408 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1396 1477 1812 3956 1396
Size: 81 335 2144 1624
Fragments arranged by size:
2144 1624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 949 1021 1108 1140 697
Size: 252 72 87 32 3741
Fragments arranged by size:
3741 252 87 72 32
Bpu10I CC'TnA_GC
Cuts at: 685 733 1021 685
Size: 48 288 3848
Fragments arranged by size:
3848 288 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1360 2239 2731 3029 934
Size: 426 879 492 298 2089
Fragments arranged by size:
2089 879 492 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 673 3888 673
Size: 3215 969
BsaAI yAC'GTr
Cuts at: 1537 1537
Size: 4184
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 112 1615 1863 2565 4061 112
Size: 1503 248 702 1496 235
Fragments arranged by size:
1503 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 256 1373 1894 2572 2846 2993 3404 256
Size: 1117 521 678 274 147 411 1036
Fragments arranged by size:
1117 1036 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 880 958 277
Size: 603 78 3503
Fragments arranged by size:
3503 603 78
BseYI C'CCAG_C
Cuts at: 305 1107 1609 2367 2944 3440 305
Size: 802 502 758 577 496 1049
Fragments arranged by size:
1049 802 758 577 502 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 742
Size: 4184
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 344 950 1164 1198 1976 2166 2958 344
Size: 606 214 34 778 190 792 1570
Fragments arranged by size:
1570 792 778 606 214 190 34
Bsp1286I G_dGCh'C
Cuts at: 146 182 344 872 950 1164 1198 1809 1902
Size: 36 162 528 78 214 34 611 93
Cuts at: 1902 1976 2166 2228 2958 146
Size: 74 190 62 730 1372
Fragments arranged by size:
1372 730 611 528 214 190 162 93
78 74 62 36 34
BspEI T'CCGG_A
Cuts at: 256 3404 256
Size: 3148 1036
BspHI T'CATG_A
Cuts at: 3360 3360
Size: 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 492 1234 1448 1750 2131 492
Size: 742 214 302 381 2545
Fragments arranged by size:
2545 742 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2476 2530 4014 4179 50
Size: 2426 54 1484 165 55
Fragments arranged by size:
2426 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2096
Size: 4184
BsrFI r'CCGG_y
Cuts at: 2182 2363 2572 2182
Size: 181 209 3794
Fragments arranged by size:
3794 209 181
BssSI C'ACGA_G
Cuts at: 658 706 1012 2455 2813 658
Size: 48 306 1443 358 2029
Fragments arranged by size:
2029 1443 358 306 48
BstAPI GCAn_nnn'nTGC
Cuts at: 1083 1561 1083
Size: 478 3706
BstBI TT'CG_AA
Cuts at: 959 2545 959
Size: 1586 2598
BstEII G'GTnAC_C
Cuts at: 504 504
Size: 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 1107 1107
Size: 4184
BstYI r'GATC_y
Cuts at: 1 1151 1209 1676 1699 2034 2280 3292 3378
Size: 1150 58 467 23 335 246 1012 86
Cuts at: 3378 3390 3736 1
Size: 12 346 449
Fragments arranged by size:
1150 1012 467 449 346 335 246 86
58 23 12
BtgI C'CryG_G
Cuts at: 107 115 198 107
Size: 8 83 4093
Fragments arranged by size:
4093 83 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 1427 1427
Size: 4184
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DrdI GACnn_nn'nnGTC
Cuts at: 1890 2748 1890
Size: 858 3326
EaeI y'GGCC_r
Cuts at: 335 464 1769 1943 2334 2361 3706 335
Size: 129 1305 174 391 27 1345 813
Fragments arranged by size:
1345 1305 813 391 174 129 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 175 2207 2417 175
Size: 2032 210 1942
Fragments arranged by size:
2032 1942 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 894 1110 2392 2702 2848 3994 894
Size: 216 1282 310 146 1146 1084
Fragments arranged by size:
1282 1146 1084 310 216 146
EcoICRI GAG'CTC
Cuts at: 342 948 1162 1196 342
Size: 606 214 34 3330
Fragments arranged by size:
3330 606 214 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 697 799 949 1021 1108 1140 2009 2441
Size: 321 102 150 72 87 32 869 432
Cuts at: 2441 3188 3456 376
Size: 747 268 1104
Fragments arranged by size:
1104 869 747 432 321 268 150 102
87 72 32
EcoNI CCTnn'n_nnAGG
Cuts at: 1087 1087
Size: 4184
EcoO109I rG'GnC_Cy
Cuts at: 179 807 891 1137 1323 179
Size: 628 84 246 186 3040
Fragments arranged by size:
3040 628 246 186 84
EcoRI G'AATT_C
Cuts at: 1188 1188
Size: 4184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 4184
FspI TGC'GCA
Cuts at: 793 823 1010 1965 793
Size: 30 187 955 3012
Fragments arranged by size:
3012 955 187 30
HaeII r_GCGC'y
Cuts at: 115 154 1618 1866 2888 3867 115
Size: 39 1464 248 1022 979 432
Fragments arranged by size:
1464 1022 979 432 248 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2026 2056 2058 2088 2026
Size: 30 2 30 4122
Fragments arranged by size:
4122 30 30 2
HincII GTy'rAC
Cuts at: 1223 1223
Size: 4184
HindIII A'AGCT_T
Cuts at: 397 397
Size: 4184
KasI G'GCGC_C
Cuts at: 111 1614 1862 111
Size: 1503 248 2433
Fragments arranged by size:
2433 1503 248
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3039 2855
Size: 184 4000
MscI TGG'CCA
Cuts at: 466 1945 466
Size: 1479 2705
MslI CAynn'nnrTG
Cuts at: 2173 2300 2173
Size: 127 4057
MspA1I CmG'CkG
Cuts at: 117 190 291 999 1295 1609 1969 2982 3227
Size: 73 101 708 296 314 360 1013 245
Cuts at: 3227 117
Size: 1074
Fragments arranged by size:
1074 1013 708 360 314 296 245 101
73
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 112 1615 1863 112
Size: 1503 248 2433
Fragments arranged by size:
2433 1503 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4184
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NspI r_CATG'y
Cuts at: 1237 1737 2644 1237
Size: 500 907 2777
Fragments arranged by size:
2777 907 500
PasI CC'CwG_GG
Cuts at: 872 872
Size: 4184
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PflMI CCAn_nnn'nTGG
Cuts at: 810 810
Size: 4184
PfoI T'CCnGG_A
Cuts at: 938 938
Size: 4184
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 844 876 3323 3355 844
Size: 32 2447 32 1673
Fragments arranged by size:
2447 1673 32 32
PpuMI rG'GwC_Cy
Cuts at: 891 1137 891
Size: 246 3938
PspOMI G'GGCC_C
Cuts at: 178 178
Size: 4184
PstI C_TGCA'G
Cuts at: 1136 1231 1916 1136
Size: 95 685 3404
Fragments arranged by size:
3404 685 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 291 999 1609 1969 291
Size: 708 610 360 2506
Fragments arranged by size:
2506 708 610 360
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 344 950 1164 1198 344
Size: 606 214 34 3330
Fragments arranged by size:
3330 606 214 34
SacII CC_GC'GG
Cuts at: 118 118
Size: 4184
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 175 2207 2417 175
Size: 2032 210 1942
Fragments arranged by size:
2032 1942 210
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 211 3701 211
Size: 3490 694
SfcI C'TryA_G
Cuts at: 33 843 1132 1227 1912 2905 3096 33
Size: 810 289 95 685 993 191 1121
Fragments arranged by size:
1121 993 810 685 289 191 95
SfoI GGC'GCC
Cuts at: 113 1616 1864 113
Size: 1503 248 2433
Fragments arranged by size:
2433 1503 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 949 1339 2254 2746 3008 949
Size: 390 915 492 262 2125
Fragments arranged by size:
2125 915 492 390 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1237 1737 1237
Size: 500 3684
StyI C'CwwG_G
Cuts at: 107 1100 1318 1672 107
Size: 993 218 354 2619
Fragments arranged by size:
2619 993 354 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 544 709 1767 1900 2359 2568 544
Size: 165 1058 133 459 209 2160
Fragments arranged by size:
2160 1058 459 209 165 133
TatI w'GTAC_w
Cuts at: 209 662 3699 209
Size: 453 3037 694
Fragments arranged by size:
3037 694 453
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 553 585 3701 3733 553
Size: 32 3116 32 1004
Fragments arranged by size:
3116 1004 32 32
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 474 1679 474
Size: 1205 2979
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI