Restriction map for
pF1KB6488
(Circular) (Six-base) MAPSORT of: pF1KB6488.seq Check: 9662 from: 1 to: 4184
pF1KB6488 4184 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 511 941 511
Size: 430 3754
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 2009 2441 3188 3456 1177
Size: 832 432 747 268 1905
Fragments arranged by size:
1905 832 747 432 268
AfeI AGC'GCT
Cuts at: 208 208
Size: 4184
AflIII A'CryG_T
Cuts at: 1536 2640 3617 1536
Size: 1104 977 2103
Fragments arranged by size:
2103 1104 977
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 973 973
Size: 4184
AlwNI CAG_nnn'CTG
Cuts at: 980 1149 1489 3056 980
Size: 169 340 1567 2108
Fragments arranged by size:
2108 1567 340 169
ApaLI G'TGCA_C
Cuts at: 498 2954 498
Size: 2456 1728
ApoI r'AATT_y
Cuts at: 472 490 1188 3537 3793 472
Size: 18 698 2349 256 863
Fragments arranged by size:
2349 863 698 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 225 262 785 1204 225
Size: 37 523 419 3205
Fragments arranged by size:
3205 523 419 37
AvrII C'CTAG_G
Cuts at: 690 690
Size: 4184
BamHI G'GATC_C
Cuts at: 1209 1209
Size: 4184
BanI G'GyrC_C
Cuts at: 594 1200 1614 1862 1897 594
Size: 606 414 248 35 2881
Fragments arranged by size:
2881 606 414 248 35
BanII G_rGCy'C
Cuts at: 1198 2228 1198
Size: 1030 3154
BbeI G_GCGC'C
Cuts at: 1618 1866 1618
Size: 248 3936
BbsI GAAGACnn'nnnn_
Cuts at: 1165 1165
Size: 4184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 131 165 90
Size: 34 7 34 4109
Fragments arranged by size:
4109 34 34 7
BciVI GTATCCnnnnn_n'
Cuts at: 2082 2843 4184 2082
Size: 761 1341 2082
Fragments arranged by size:
2082 1341 761
BclI T'GATC_A
Cuts at: 1704 1704
Size: 4184
BglI GCCn_nnn'nGGC
Cuts at: 320 320
Size: 4184
BglII A'GATC_T
Cuts at: 336 1699 3736 336
Size: 1363 2037 784
Fragments arranged by size:
2037 1363 784
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 226 238 312 502 533 1809 1902 2958 226
Size: 12 74 190 31 1276 93 1056 1452
Fragments arranged by size:
1452 1276 1056 190 93 74 31 12
BmrI ACTGGGnnnn_n'
Cuts at: 213 658 1396 1477 1812 3956 213
Size: 445 738 81 335 2144 441
Fragments arranged by size:
2144 738 445 441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 348 2551 348
Size: 2203 1981
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 620 1126 1171 361
Size: 259 506 45 3374
Fragments arranged by size:
3374 506 259 45
Bpu10I CC'TnA_GC
Cuts at: 560 560
Size: 4184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 1360 2239 2731 3029 696
Size: 664 879 492 298 1851
Fragments arranged by size:
1851 879 664 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3888 3888
Size: 4184
BsaAI yAC'GTr
Cuts at: 1537 1537
Size: 4184
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 244 1615 1863 2565 4061 244
Size: 1371 248 702 1496 367
Fragments arranged by size:
1496 1371 702 367 248
BsaWI w'CCGG_w
Cuts at: 1373 1894 2572 2846 2993 3404 1373
Size: 521 678 274 147 411 2153
Fragments arranged by size:
2153 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 686 716 686
Size: 30 4154
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 652 652
Size: 4184
BseYI C'CCAG_C
Cuts at: 391 573 1609 2367 2944 3440 391
Size: 182 1036 758 577 496 1135
Fragments arranged by size:
1135 1036 758 577 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 396 933 943 993 1083 139
Size: 257 537 10 50 90 3240
Fragments arranged by size:
3240 537 257 90 50 10
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 127 502 1054 1198 1976 2166 2958 127
Size: 375 552 144 778 190 792 1353
Fragments arranged by size:
1353 792 778 552 375 190 144
Bsp1286I G_dGCh'C
Cuts at: 127 226 238 312 502 533 1054 1198 1809
Size: 99 12 74 190 31 521 144 611
Cuts at: 1809 1902 1976 2166 2228 2958 127
Size: 93 74 190 62 730 1353
Fragments arranged by size:
1353 730 611 521 190 190 144 99
93 74 74 62 31 12
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 3360 3360
Size: 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 262 511 941 1105 1234 1448 1750 2131 262
Size: 249 430 164 129 214 302 381 2315
Fragments arranged by size:
2315 430 381 302 249 214 164 129
BsrBI CCG'CTC
Cuts at: 50 2476 2530 4014 4179 50
Size: 2426 54 1484 165 55
Fragments arranged by size:
2426 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2096
Size: 4184
BsrFI r'CCGG_y
Cuts at: 2182 2363 2572 2182
Size: 181 209 3794
Fragments arranged by size:
3794 209 181
BssHII G'CGCG_C
Cuts at: 270 270
Size: 4184
BssSI C'ACGA_G
Cuts at: 126 2455 2813 126
Size: 2329 358 1497
Fragments arranged by size:
2329 1497 358
BstAPI GCAn_nnn'nTGC
Cuts at: 117 1561 117
Size: 1444 2740
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstEII G'GTnAC_C
Cuts at: 789 789
Size: 4184
BstYI r'GATC_y
Cuts at: 1 336 620 1209 1676 1699 2034 2280 3292
Size: 335 284 589 467 23 335 246 1012
Cuts at: 3292 3378 3390 3736 1
Size: 86 12 346 449
Fragments arranged by size:
1012 589 467 449 346 335 335 284
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 154 366 468 999 1427 154
Size: 212 102 531 428 2911
Fragments arranged by size:
2911 531 428 212 102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 747 782 747
Size: 35 4149
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DraIII CAC_nnn'GTG
Cuts at: 743 743
Size: 4184
DrdI GACnn_nn'nnGTC
Cuts at: 715 1890 2748 715
Size: 1175 858 2151
Fragments arranged by size:
2151 1175 858
EaeI y'GGCC_r
Cuts at: 999 1769 1943 2334 2361 3706 999
Size: 770 174 391 27 1345 1477
Fragments arranged by size:
1477 1345 770 391 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2392 2702 2848 3994 2392
Size: 310 146 1146 2582
Fragments arranged by size:
2582 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1196 1196
Size: 4184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 620 1126 1171 1177 2009 2441 3188 3456
Size: 259 506 45 6 832 432 747 268
Cuts at: 3456 361
Size: 1089
Fragments arranged by size:
1089 832 747 506 432 268 259 45
6
EcoNI CCTnn'n_nnAGG
Cuts at: 187 187
Size: 4184
EcoO109I rG'GnC_Cy
Cuts at: 1094 1323 1094
Size: 229 3955
EcoRI G'AATT_C
Cuts at: 1188 1188
Size: 4184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 4184
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 210 1618 1866 2888 3867 210
Size: 1408 248 1022 979 527
Fragments arranged by size:
1408 1022 979 527 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 120 152 274 306 686 718 2026 2056 2058
Size: 32 122 32 380 32 1308 30 2
Cuts at: 2058 2088 120
Size: 30 2216
Fragments arranged by size:
2216 1308 380 122 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 720 1223 720
Size: 503 3681
KasI G'GCGC_C
Cuts at: 1614 1862 1614
Size: 248 3936
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3039 2855
Size: 184 4000
MscI TGG'CCA
Cuts at: 1945 1945
Size: 4184
MslI CAynn'nnrTG
Cuts at: 2173 2300 2173
Size: 127 4057
MspA1I CmG'CkG
Cuts at: 536 543 1004 1295 1609 1969 2982 3227 536
Size: 7 461 291 314 360 1013 245 1493
Fragments arranged by size:
1493 1013 461 360 314 291 245 7
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 1615 1863 1615
Size: 248 3936
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NspI r_CATG'y
Cuts at: 755 1237 1737 2644 755
Size: 482 500 907 2295
Fragments arranged by size:
2295 907 500 482
PasI CC'CwG_GG
Cuts at: 1057 1057
Size: 4184
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PflMI CCAn_nnn'nTGG
Cuts at: 183 743 1149 183
Size: 560 406 3218
Fragments arranged by size:
3218 560 406
PfoI T'CCnGG_A
Cuts at: 1142 1142
Size: 4184
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4152
PpuMI rG'GwC_Cy
Cuts at: 1094 1094
Size: 4184
PstI C_TGCA'G
Cuts at: 508 938 994 1231 1916 508
Size: 430 56 237 685 2776
Fragments arranged by size:
2776 685 430 237 56
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 543 1609 1969 543
Size: 1066 360 2758
Fragments arranged by size:
2758 1066 360
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 1198 1198
Size: 4184
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 3701 3701
Size: 4184
SfcI C'TryA_G
Cuts at: 33 504 934 990 1227 1912 2905 3096 33
Size: 471 430 56 237 685 993 191 1121
Fragments arranged by size:
1121 993 685 471 430 237 191 56
SfoI GGC'GCC
Cuts at: 1616 1864 1616
Size: 248 3936
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 227 264 787 1206 227
Size: 37 523 419 3205
Fragments arranged by size:
3205 523 419 37
SmlI C'TyrA_G
Cuts at: 711 1339 2254 2746 3008 711
Size: 628 915 492 262 1887
Fragments arranged by size:
1887 915 628 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 755 1237 1737 755
Size: 482 500 3202
Fragments arranged by size:
3202 500 482
SrfI GCCC'GGGC
Cuts at: 227 227
Size: 4184
StuI AGG'CCT
Cuts at: 656 1135 656
Size: 479 3705
StyI C'CwwG_G
Cuts at: 690 1318 1672 690
Size: 628 354 3202
Fragments arranged by size:
3202 628 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1767 1900 2359 2568 1767
Size: 133 459 209 3383
Fragments arranged by size:
3383 459 209 133
TatI w'GTAC_w
Cuts at: 3699 3699
Size: 4184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1101 1133 3701 3733 1101
Size: 32 2568 32 1552
Fragments arranged by size:
2568 1552 32 32
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1173 1679 1173
Size: 506 3678
XmaI C'CCGG_G
Cuts at: 225 262 785 1204 225
Size: 37 523 419 3205
Fragments arranged by size:
3205 523 419 37
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI BaeI
BbvCI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BstXI BstZ17I Bsu36I BtgI ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SspI SwaI
XhoI XmnI ZraI