Restriction map for
pF1KB6486
(Circular) (Six-base) MAPSORT of: pF1KB6486.seq Check: 9192 from: 1 to: 4181
pF1KB6486 4181 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 699 699
Size: 4181
AccI GT'mk_AC
Cuts at: 1219 1219
Size: 4181
Acc65I G'GTAC_C
Cuts at: 1197 1197
Size: 4181
AclI AA'CG_TT
Cuts at: 4021 4021
Size: 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 2006 2438 3185 3453 808
Size: 1198 432 747 268 1536
Fragments arranged by size:
1536 1198 747 432 268
AflII C'TTAA_G
Cuts at: 820 820
Size: 4181
AflIII A'CryG_T
Cuts at: 1533 2637 3614 1533
Size: 1104 977 2100
Fragments arranged by size:
2100 1104 977
AgeI A'CCGG_T
Cuts at: 2569 2569
Size: 4181
AlwNI CAG_nnn'CTG
Cuts at: 1122 1125 1486 3053 1122
Size: 3 361 1567 2250
Fragments arranged by size:
2250 1567 361 3
ApaLI G'TGCA_C
Cuts at: 2951 2951
Size: 4181
ApoI r'AATT_y
Cuts at: 120 987 1185 3534 3790 120
Size: 867 198 2349 256 511
Fragments arranged by size:
2349 867 511 256 198
AseI AT'TA_AT
Cuts at: 20 20
Size: 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
AvaI C'yCGr_G
Cuts at: 1201 1201
Size: 4181
BamHI G'GATC_C
Cuts at: 1206 1206
Size: 4181
BanI G'GyrC_C
Cuts at: 188 1197 1611 1859 1894 188
Size: 1009 414 248 35 2475
Fragments arranged by size:
2475 1009 414 248 35
BanII G_rGCy'C
Cuts at: 1085 1195 2225 1085
Size: 110 1030 3041
Fragments arranged by size:
3041 1030 110
BbeI G_GCGC'C
Cuts at: 1615 1863 1615
Size: 248 3933
BbvCI CC'TCA_GC
Cuts at: 545 1139 545
Size: 594 3587
BciVI GTATCCnnnnn_n'
Cuts at: 2079 2840 4181 2079
Size: 761 1341 2079
Fragments arranged by size:
2079 1341 761
BclI T'GATC_A
Cuts at: 938 1701 938
Size: 763 3418
BglII A'GATC_T
Cuts at: 1696 3733 1696
Size: 2037 2144
BlpI GC'TnA_GC
Cuts at: 1293 1293
Size: 4181
Bme1580I G_kGCm'C
Cuts at: 599 1806 1899 2955 599
Size: 1207 93 1056 1825
Fragments arranged by size:
1825 1207 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1393 1474 1809 3953 1393
Size: 81 335 2144 1621
Fragments arranged by size:
2144 1621 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 4181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 669 162
Size: 507 3674
Bpu10I CC'TnA_GC
Cuts at: 536 545 1079 1139 536
Size: 9 534 60 3578
Fragments arranged by size:
3578 534 60 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 2236 2728 3026 1357
Size: 879 492 298 2512
Fragments arranged by size:
2512 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 238 3885 238
Size: 3647 534
BsaAI yAC'GTr
Cuts at: 1534 1534
Size: 4181
BsaBI GATnn'nnATC
Cuts at: 6 1695 3732 6
Size: 1689 2037 455
Fragments arranged by size:
2037 1689 455
BsaHI Gr'CG_yC
Cuts at: 1612 1860 2562 4058 1612
Size: 248 702 1496 1735
Fragments arranged by size:
1735 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1370 1891 2569 2843 2990 3401 1370
Size: 521 678 274 147 411 2150
Fragments arranged by size:
2150 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 550 865 1606 2364 2941 3437 550
Size: 315 741 758 577 496 1294
Fragments arranged by size:
1294 758 741 577 496 315
BsiEI CG_ry'CG
Cuts at: 104 1723 1769 2977 104
Size: 1619 46 1208 1308
Fragments arranged by size:
1619 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1085 1195 1973 2163 2955 1085
Size: 110 778 190 792 2311
Fragments arranged by size:
2311 792 778 190 110
BsmBI CGTCTCn'nnnn_
Cuts at: 135 135
Size: 4181
Bsp1286I G_dGCh'C
Cuts at: 599 1085 1195 1806 1899 1973 2163 2225 2955
Size: 486 110 611 93 74 190 62 730
Cuts at: 2955 599
Size: 1825
Fragments arranged by size:
1825 730 611 486 190 110 93 74
62
BspEI T'CCGG_A
Cuts at: 3401 3401
Size: 4181
BspHI T'CATG_A
Cuts at: 1107 3357 1107
Size: 2250 1931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 318 681 699 1231 1445 1747 2128 318
Size: 363 18 532 214 302 381 2371
Fragments arranged by size:
2371 532 381 363 302 214 18
BsrBI CCG'CTC
Cuts at: 50 2473 2527 4011 4176 50
Size: 2423 54 1484 165 55
Fragments arranged by size:
2423 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2093 2093
Size: 4181
BsrFI r'CCGG_y
Cuts at: 185 2179 2360 2569 185
Size: 1994 181 209 1797
Fragments arranged by size:
1994 1797 209 181
BsrGI T'GTAC_A
Cuts at: 924 924
Size: 4181
BssSI C'ACGA_G
Cuts at: 2452 2810 2452
Size: 358 3823
BstAPI GCAn_nnn'nTGC
Cuts at: 1125 1558 1125
Size: 433 3748
BstBI TT'CG_AA
Cuts at: 2542 2542
Size: 4181
BstYI r'GATC_y
Cuts at: 1 1206 1673 1696 2031 2277 3289 3375 3387
Size: 1205 467 23 335 246 1012 86 12
Cuts at: 3387 3733 1
Size: 346 449
Fragments arranged by size:
1205 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 766 766
Size: 4181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2131 2312 2475 2131
Size: 181 163 3837
Fragments arranged by size:
3837 181 163
BtsI GCAGTG_nn'
Cuts at: 1424 1424
Size: 4181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 333 368 333
Size: 35 4146
DraI TTT'AAA
Cuts at: 1180 1180
Size: 4181
DraIII CAC_nnn'GTG
Cuts at: 417 837 417
Size: 420 3761
DrdI GACnn_nn'nnGTC
Cuts at: 1887 2745 1887
Size: 858 3323
EaeI y'GGCC_r
Cuts at: 399 582 797 1766 1940 2331 2358 3703 399
Size: 183 215 969 174 391 27 1345 877
Fragments arranged by size:
1345 969 877 391 215 183 174 27
EagI C'GGCC_G
Cuts at: 1766 1766
Size: 4181
EarI CTCTTCn'nnn_
Cuts at: 449 484 2204 2414 449
Size: 35 1720 210 2216
Fragments arranged by size:
2216 1720 210 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2389 2699 2845 3991 2389
Size: 310 146 1146 2579
Fragments arranged by size:
2579 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1083 1193 1083
Size: 110 4071
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 669 808 2006 2438 3185 3453 162
Size: 507 139 1198 432 747 268 890
Fragments arranged by size:
1198 890 747 507 432 268 139
EcoNI CCTnn'n_nnAGG
Cuts at: 604 604
Size: 4181
EcoO109I rG'GnC_Cy
Cuts at: 678 1320 678
Size: 642 3539
EcoRI G'AATT_C
Cuts at: 987 1185 987
Size: 198 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 4181
FspI TGC'GCA
Cuts at: 1962 1962
Size: 4181
HaeII r_GCGC'y
Cuts at: 1615 1863 2885 3864 1615
Size: 248 1022 979 1932
Fragments arranged by size:
1932 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1046 1078 2023 2053 2055 2085 1046
Size: 32 945 30 2 30 3142
Fragments arranged by size:
3142 945 32 30 30 2
HincII GTy'rAC
Cuts at: 1220 1220
Size: 4181
KasI G'GCGC_C
Cuts at: 1611 1859 1611
Size: 248 3933
KpnI G_GTAC'C
Cuts at: 1201 1201
Size: 4181
MluI A'CGCG_T
Cuts at: 3614 3614
Size: 4181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 158 2852 3036 158
Size: 2694 184 1303
Fragments arranged by size:
2694 1303 184
MscI TGG'CCA
Cuts at: 401 584 799 1942 401
Size: 183 215 1143 2640
Fragments arranged by size:
2640 1143 215 183
MslI CAynn'nnrTG
Cuts at: 932 2170 2297 932
Size: 1238 127 2816
Fragments arranged by size:
2816 1238 127
MspA1I CmG'CkG
Cuts at: 1122 1143 1292 1606 1966 2979 3224 1122
Size: 21 149 314 360 1013 245 2079
Fragments arranged by size:
2079 1013 360 314 245 149 21
NaeI GCC'GGC
Cuts at: 187 2362 187
Size: 2175 2006
NarI GG'CG_CC
Cuts at: 1612 1860 1612
Size: 248 3933
NdeI CA'TA_TG
Cuts at: 827 827
Size: 4181
NgoMIV G'CCGG_C
Cuts at: 185 2360 185
Size: 2175 2006
NspI r_CATG'y
Cuts at: 1234 1734 2641 1234
Size: 500 907 2774
Fragments arranged by size:
2774 907 500
PasI CC'CwG_GG
Cuts at: 639 639
Size: 4181
PciI A'CATG_T
Cuts at: 2637 2637
Size: 4181
PflMI CCAn_nnn'nTGG
Cuts at: 756 756
Size: 4181
PmeI GTTT'AAAC
Cuts at: 1180 1180
Size: 4181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 4149
PpuMI rG'GwC_Cy
Cuts at: 678 678
Size: 4181
PstI C_TGCA'G
Cuts at: 395 1228 1913 395
Size: 833 685 2663
Fragments arranged by size:
2663 833 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4181
PvuII CAG'CTG
Cuts at: 1122 1143 1606 1966 1122
Size: 21 463 360 3337
Fragments arranged by size:
3337 463 360 21
RsrII CG'GwC_CG
Cuts at: 2376 2376
Size: 4181
SacI G_AGCT'C
Cuts at: 1085 1195 1085
Size: 110 4071
SalI G'TCGA_C
Cuts at: 1218 1218
Size: 4181
SapI GCTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
SbfI CC_TGCA'GG
Cuts at: 1228 1228
Size: 4181
ScaI AGT'ACT
Cuts at: 3698 3698
Size: 4181
SfcI C'TryA_G
Cuts at: 33 391 518 1224 1909 2902 3093 33
Size: 358 127 706 685 993 191 1121
Fragments arranged by size:
1121 993 706 685 358 191 127
SfoI GGC'GCC
Cuts at: 1613 1861 1613
Size: 248 3933
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
SmaI CCC'GGG
Cuts at: 1203 1203
Size: 4181
SmlI C'TyrA_G
Cuts at: 820 1336 2251 2743 3005 820
Size: 516 915 492 262 1996
Fragments arranged by size:
1996 915 516 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4181
SphI G_CATG'C
Cuts at: 1234 1734 1234
Size: 500 3681
StyI C'CwwG_G
Cuts at: 1315 1669 1315
Size: 354 3827
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1764 1897 2356 2565 1764
Size: 133 459 209 3380
Fragments arranged by size:
3380 459 209 133
TatI w'GTAC_w
Cuts at: 282 924 3696 282
Size: 642 2772 767
Fragments arranged by size:
2772 767 642
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4149
Tth111I GACn'n_nGTC
Cuts at: 358 1978 358
Size: 1620 2561
XbaI T'CTAG_A
Cuts at: 1212 1212
Size: 4181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1676 1676
Size: 4181
XmaI C'CCGG_G
Cuts at: 1201 1201
Size: 4181
XmnI GAAnn'nnTTC
Cuts at: 991 991
Size: 4181
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BsmI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI