Restriction map for
pF1KB6482
(Circular) (Six-base) MAPSORT of: pF1KB6482.seq Check: 5868 from: 1 to: 4181
pF1KB6482 4181 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1219 1219
Size: 4181
Acc65I G'GTAC_C
Cuts at: 1197 1197
Size: 4181
AclI AA'CG_TT
Cuts at: 4021 4021
Size: 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 2006 2438 3185 3453 532
Size: 1474 432 747 268 1260
Fragments arranged by size:
1474 1260 747 432 268
AflIII A'CryG_T
Cuts at: 1533 2637 3614 1533
Size: 1104 977 2100
Fragments arranged by size:
2100 1104 977
AgeI A'CCGG_T
Cuts at: 2569 2569
Size: 4181
AleI CACnn'nnGTG
Cuts at: 530 530
Size: 4181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 967 967
Size: 4181
AlwNI CAG_nnn'CTG
Cuts at: 1486 3053 1486
Size: 1567 2614
ApaI G_GGCC'C
Cuts at: 232 604 232
Size: 372 3809
ApaLI G'TGCA_C
Cuts at: 989 2951 989
Size: 1962 2219
ApoI r'AATT_y
Cuts at: 1185 3534 3790 1185
Size: 2349 256 1576
Fragments arranged by size:
2349 1576 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
AvaI C'yCGr_G
Cuts at: 231 1009 1201 231
Size: 778 192 3211
Fragments arranged by size:
3211 778 192
AvrII C'CTAG_G
Cuts at: 384 384
Size: 4181
BamHI G'GATC_C
Cuts at: 1206 1206
Size: 4181
BanI G'GyrC_C
Cuts at: 457 563 1197 1611 1859 1894 457
Size: 106 634 414 248 35 2744
Fragments arranged by size:
2744 634 414 248 106 35
BanII G_rGCy'C
Cuts at: 175 232 249 604 821 838 1002 1010 1195
Size: 57 17 355 217 17 164 8 185
Cuts at: 1195 2225 175
Size: 1030 2131
Fragments arranged by size:
2131 1030 355 217 185 164 57 17
17 8
BbeI G_GCGC'C
Cuts at: 461 567 1615 1863 461
Size: 106 1048 248 2779
Fragments arranged by size:
2779 1048 248 106
BbsI GAAGACnn'nnnn_
Cuts at: 249 249
Size: 4181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 921 955 921
Size: 34 4147
BciVI GTATCCnnnnn_n'
Cuts at: 2079 2840 4181 2079
Size: 761 1341 2079
Fragments arranged by size:
2079 1341 761
BclI T'GATC_A
Cuts at: 1701 1701
Size: 4181
BglII A'GATC_T
Cuts at: 1696 3733 1696
Size: 2037 2144
BlpI GC'TnA_GC
Cuts at: 1293 1293
Size: 4181
Bme1580I G_kGCm'C
Cuts at: 232 525 537 543 604 993 1806 1899 2955
Size: 293 12 6 61 389 813 93 1056
Cuts at: 2955 232
Size: 1458
Fragments arranged by size:
1458 1056 813 389 293 93 61 12
6
BmrI ACTGGGnnnn_n'
Cuts at: 1393 1474 1809 3953 1393
Size: 81 335 2144 1621
Fragments arranged by size:
2144 1621 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 4181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 815 898 727
Size: 88 83 4010
Fragments arranged by size:
4010 88 83
Bpu10I CC'TnA_GC
Cuts at: 596 1004 596
Size: 408 3773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 2236 2728 3026 1357
Size: 879 492 298 2512
Fragments arranged by size:
2512 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3885 3885
Size: 4181
BsaAI yAC'GTr
Cuts at: 744 824 1534 744
Size: 80 710 3391
Fragments arranged by size:
3391 710 80
BsaBI GATnn'nnATC
Cuts at: 6 1695 3732 6
Size: 1689 2037 455
Fragments arranged by size:
2037 1689 455
BsaHI Gr'CG_yC
Cuts at: 458 564 1612 1860 2562 4058 458
Size: 106 1048 248 702 1496 581
Fragments arranged by size:
1496 1048 702 581 248 106
BsaWI w'CCGG_w
Cuts at: 183 813 1370 1891 2569 2843 2990 3401 183
Size: 630 557 521 678 274 147 411 963
Fragments arranged by size:
963 678 630 557 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 430 460 808 838 430
Size: 30 348 30 3773
Fragments arranged by size:
3773 348 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 691 700 973 691
Size: 9 273 3899
Fragments arranged by size:
3899 273 9
BseYI C'CCAG_C
Cuts at: 112 1137 1606 2364 2941 3437 112
Size: 1025 469 758 577 496 856
Fragments arranged by size:
1025 856 758 577 496 469
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 385 484 277
Size: 108 99 3974
Fragments arranged by size:
3974 108 99
BsiEI CG_ry'CG
Cuts at: 104 353 831 1723 1769 2977 104
Size: 249 478 892 46 1208 1308
Fragments arranged by size:
1308 1208 892 478 249 46
BsiHKAI G_wGCw'C
Cuts at: 993 1195 1973 2163 2955 993
Size: 202 778 190 792 2219
Fragments arranged by size:
2219 792 778 202 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1061 1061
Size: 4181
Bsp1286I G_dGCh'C
Cuts at: 175 232 249 525 537 543 604 821 838
Size: 57 17 276 12 6 61 217 17
Cuts at: 838 993 1002 1010 1195 1806 1899 1973 2163
Size: 155 9 8 185 611 93 74 190
Cuts at: 2163 2225 2955 175
Size: 62 730 1401
Fragments arranged by size:
1401 730 611 276 217 190 185 155
93 74 62 61 57 17 17 12
9 8 6
BspEI T'CCGG_A
Cuts at: 183 3401 183
Size: 3218 963
BspHI T'CATG_A
Cuts at: 3357 3357
Size: 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 380 1231 1445 1747 2128 380
Size: 851 214 302 381 2433
Fragments arranged by size:
2433 851 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2473 2527 4011 4176 50
Size: 2423 54 1484 165 55
Fragments arranged by size:
2423 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2093 2093
Size: 4181
BsrFI r'CCGG_y
Cuts at: 352 2179 2360 2569 352
Size: 1827 181 209 1964
Fragments arranged by size:
1964 1827 209 181
BssSI C'ACGA_G
Cuts at: 2452 2810 2452
Size: 358 3823
BstAPI GCAn_nnn'nTGC
Cuts at: 1558 1558
Size: 4181
BstBI TT'CG_AA
Cuts at: 2542 2542
Size: 4181
BstYI r'GATC_y
Cuts at: 1 1206 1673 1696 2031 2277 3289 3375 3387
Size: 1205 467 23 335 246 1012 86 12
Cuts at: 3387 3733 1
Size: 346 449
Fragments arranged by size:
1205 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 889 889
Size: 4181
BtgI C'CryG_G
Cuts at: 107 431 1172 107
Size: 324 741 3116
Fragments arranged by size:
3116 741 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1091 2131 2312 2475 1091
Size: 1040 181 163 2797
Fragments arranged by size:
2797 1040 181 163
BtsI GCAGTG_nn'
Cuts at: 289 857 951 1424 289
Size: 568 94 473 3046
Fragments arranged by size:
3046 568 473 94
DraI TTT'AAA
Cuts at: 1180 1180
Size: 4181
DrdI GACnn_nn'nnGTC
Cuts at: 1887 2745 1887
Size: 858 3323
EaeI y'GGCC_r
Cuts at: 350 746 828 927 1766 1940 2331 2358 3703
Size: 396 82 99 839 174 391 27 1345
Cuts at: 3703 350
Size: 828
Fragments arranged by size:
1345 839 828 396 391 174 99 82
27
EagI C'GGCC_G
Cuts at: 350 828 1766 350
Size: 478 938 2765
Fragments arranged by size:
2765 938 478
EarI CTCTTCn'nnn_
Cuts at: 668 1157 2204 2414 668
Size: 489 1047 210 2435
Fragments arranged by size:
2435 1047 489 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 490 2389 2699 2845 3991 490
Size: 1899 310 146 1146 680
Fragments arranged by size:
1899 1146 680 310 146
EcoICRI GAG'CTC
Cuts at: 1193 1193
Size: 4181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 727 815 898 2006 2438 3185 3453 532
Size: 195 88 83 1108 432 747 268 1260
Fragments arranged by size:
1260 1108 747 432 268 195 88 83
EcoNI CCTnn'n_nnAGG
Cuts at: 595 595
Size: 4181
EcoO109I rG'GnC_Cy
Cuts at: 205 600 601 1320 205
Size: 395 1 719 3066
Fragments arranged by size:
3066 719 395 1
EcoRI G'AATT_C
Cuts at: 1185 1185
Size: 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 4181
FspI TGC'GCA
Cuts at: 1962 1962
Size: 4181
HaeII r_GCGC'y
Cuts at: 461 499 567 1615 1863 2885 3864 461
Size: 38 68 1048 248 1022 979 778
Fragments arranged by size:
1048 1022 979 778 248 68 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1012 1044 2023 2053 2055 2085 1012
Size: 32 979 30 2 30 3108
Fragments arranged by size:
3108 979 32 30 30 2
HincII GTy'rAC
Cuts at: 1220 1220
Size: 4181
KasI G'GCGC_C
Cuts at: 457 563 1611 1859 457
Size: 106 1048 248 2779
Fragments arranged by size:
2779 1048 248 106
KpnI G_GTAC'C
Cuts at: 1201 1201
Size: 4181
MluI A'CGCG_T
Cuts at: 3614 3614
Size: 4181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 680 2852 3036 680
Size: 2172 184 1825
Fragments arranged by size:
2172 1825 184
MscI TGG'CCA
Cuts at: 1942 1942
Size: 4181
MslI CAynn'nnrTG
Cuts at: 314 530 761 2170 2297 314
Size: 216 231 1409 127 2198
Fragments arranged by size:
2198 1409 231 216 127
MspA1I CmG'CkG
Cuts at: 164 193 705 1064 1292 1606 1966 2979 3224
Size: 29 512 359 228 314 360 1013 245
Cuts at: 3224 164
Size: 1121
Fragments arranged by size:
1121 1013 512 360 359 314 245 228
29
NaeI GCC'GGC
Cuts at: 354 2362 354
Size: 2008 2173
NarI GG'CG_CC
Cuts at: 458 564 1612 1860 458
Size: 106 1048 248 2779
Fragments arranged by size:
2779 1048 248 106
NcoI C'CATG_G
Cuts at: 107 107
Size: 4181
NgoMIV G'CCGG_C
Cuts at: 352 2360 352
Size: 2008 2173
NspI r_CATG'y
Cuts at: 921 1234 1734 2641 921
Size: 313 500 907 2461
Fragments arranged by size:
2461 907 500 313
PasI CC'CwG_GG
Cuts at: 177 569 177
Size: 392 3789
PciI A'CATG_T
Cuts at: 2637 2637
Size: 4181
PfoI T'CCnGG_A
Cuts at: 326 326
Size: 4181
PmeI GTTT'AAAC
Cuts at: 1180 1180
Size: 4181
PmlI CAC'GTG
Cuts at: 744 824 744
Size: 80 4101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 4149
PpuMI rG'GwC_Cy
Cuts at: 205 205
Size: 4181
PspOMI G'GGCC_C
Cuts at: 228 600 228
Size: 372 3809
PstI C_TGCA'G
Cuts at: 377 704 1042 1228 1913 377
Size: 327 338 186 685 2645
Fragments arranged by size:
2645 685 338 327 186
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4181
PvuII CAG'CTG
Cuts at: 705 1606 1966 705
Size: 901 360 2920
Fragments arranged by size:
2920 901 360
RsrII CG'GwC_CG
Cuts at: 2376 2376
Size: 4181
SacI G_AGCT'C
Cuts at: 1195 1195
Size: 4181
SalI G'TCGA_C
Cuts at: 1218 1218
Size: 4181
SapI GCTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
SbfI CC_TGCA'GG
Cuts at: 1228 1228
Size: 4181
ScaI AGT'ACT
Cuts at: 3698 3698
Size: 4181
SfcI C'TryA_G
Cuts at: 33 373 700 1038 1224 1909 2902 3093 33
Size: 340 327 338 186 685 993 191 1121
Fragments arranged by size:
1121 993 685 340 338 327 191 186
SfoI GGC'GCC
Cuts at: 459 565 1613 1861 459
Size: 106 1048 248 2779
Fragments arranged by size:
2779 1048 248 106
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
SmaI CCC'GGG
Cuts at: 233 1203 233
Size: 970 3211
SmlI C'TyrA_G
Cuts at: 1336 2251 2743 3005 1336
Size: 915 492 262 2512
Fragments arranged by size:
2512 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4181
SphI G_CATG'C
Cuts at: 1234 1734 1234
Size: 500 3681
StyI C'CwwG_G
Cuts at: 107 384 1315 1669 107
Size: 277 931 354 2619
Fragments arranged by size:
2619 931 354 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1764 1897 2356 2565 1764
Size: 133 459 209 3380
Fragments arranged by size:
3380 459 209 133
TatI w'GTAC_w
Cuts at: 362 3696 362
Size: 3334 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 808 840 3698 3730 808
Size: 32 2858 32 1259
Fragments arranged by size:
2858 1259 32 32
Tth111I GACn'n_nGTC
Cuts at: 1978 1978
Size: 4181
XbaI T'CTAG_A
Cuts at: 1212 1212
Size: 4181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1676 1676
Size: 4181
XmaI C'CCGG_G
Cuts at: 231 1201 231
Size: 970 3211
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI BaeI
BbvCI BfrBI BglI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI