Restriction map for
pF1KB6479
(Circular) (Six-base) MAPSORT of: pF1KB6479.seq Check: 13 from: 1 to: 4094
pF1KB6479 4094 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 637 1132 637
Size: 495 3599
Acc65I G'GTAC_C
Cuts at: 1110 1110
Size: 4094
AclI AA'CG_TT
Cuts at: 3934 3934
Size: 4094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 293 1919 2351 3098 3366 208
Size: 85 1626 432 747 268 936
Fragments arranged by size:
1626 936 747 432 268 85
AflIII A'CryG_T
Cuts at: 1446 2550 3527 1446
Size: 1104 977 2013
Fragments arranged by size:
2013 1104 977
AgeI A'CCGG_T
Cuts at: 2482 2482
Size: 4094
AhdI GACnn_n'nnGTC
Cuts at: 633 633
Size: 4094
AlwNI CAG_nnn'CTG
Cuts at: 1399 2966 1399
Size: 1567 2527
ApaLI G'TGCA_C
Cuts at: 587 2864 587
Size: 2277 1817
ApoI r'AATT_y
Cuts at: 154 880 1042 1056 1098 3447 3703 154
Size: 726 162 14 42 2349 256 545
Fragments arranged by size:
2349 726 545 256 162 42 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4094
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
AvaI C'yCGr_G
Cuts at: 1114 1114
Size: 4094
BamHI G'GATC_C
Cuts at: 1119 1119
Size: 4094
BanI G'GyrC_C
Cuts at: 618 1110 1524 1772 1807 618
Size: 492 414 248 35 2905
Fragments arranged by size:
2905 492 414 248 35
BanII G_rGCy'C
Cuts at: 613 1108 2138 613
Size: 495 1030 2569
Fragments arranged by size:
2569 1030 495
BbeI G_GCGC'C
Cuts at: 622 1528 1776 622
Size: 906 248 2940
Fragments arranged by size:
2940 906 248
BbsI GAAGACnn'nnnn_
Cuts at: 214 274 214
Size: 60 4034
BciVI GTATCCnnnnn_n'
Cuts at: 191 1992 2753 4094 191
Size: 1801 761 1341 191
Fragments arranged by size:
1801 1341 761 191
BclI T'GATC_A
Cuts at: 1614 1614
Size: 4094
BglII A'GATC_T
Cuts at: 941 1609 3646 941
Size: 668 2037 1389
Fragments arranged by size:
2037 1389 668
BlpI GC'TnA_GC
Cuts at: 1206 1206
Size: 4094
Bme1580I G_kGCm'C
Cuts at: 591 1719 1812 2868 591
Size: 1128 93 1056 1817
Fragments arranged by size:
1817 1128 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 711 1306 1387 1722 3866 711
Size: 595 81 335 2144 939
Fragments arranged by size:
2144 939 595 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1046 1015
Size: 31 4063
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 2149 2641 2939 1270
Size: 879 492 298 2425
Fragments arranged by size:
2425 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3798 3798
Size: 4094
BsaAI yAC'GTr
Cuts at: 745 1447 745
Size: 702 3392
BsaBI GATnn'nnATC
Cuts at: 6 1608 3645 6
Size: 1602 2037 455
Fragments arranged by size:
2037 1602 455
BsaHI Gr'CG_yC
Cuts at: 619 1525 1773 2475 3971 619
Size: 906 248 702 1496 742
Fragments arranged by size:
1496 906 742 702 248
BsaWI w'CCGG_w
Cuts at: 429 1283 1804 2482 2756 2903 3314 429
Size: 854 521 678 274 147 411 1209
Fragments arranged by size:
1209 854 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 246 346 246
Size: 100 3994
BseYI C'CCAG_C
Cuts at: 1519 2277 2854 3350 1519
Size: 758 577 496 2263
Fragments arranged by size:
2263 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1636 1682 2890 104
Size: 1532 46 1208 1308
Fragments arranged by size:
1532 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 591 613 1108 1886 2076 2868 591
Size: 22 495 778 190 792 1817
Fragments arranged by size:
1817 792 778 495 190 22
Bsp1286I G_dGCh'C
Cuts at: 591 613 1108 1719 1812 1886 2076 2138 2868
Size: 22 495 611 93 74 190 62 730
Cuts at: 2868 591
Size: 1817
Fragments arranged by size:
1817 730 611 495 190 93 74 62
22
BspEI T'CCGG_A
Cuts at: 3314 3314
Size: 4094
BspHI T'CATG_A
Cuts at: 3270 3270
Size: 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1144 1358 1660 2041 1144
Size: 214 302 381 3197
Fragments arranged by size:
3197 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2386 2440 3924 4089 50
Size: 2336 54 1484 165 55
Fragments arranged by size:
2336 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 665 2006 665
Size: 1341 2753
BsrFI r'CCGG_y
Cuts at: 2092 2273 2482 2092
Size: 181 209 3704
Fragments arranged by size:
3704 209 181
BssSI C'ACGA_G
Cuts at: 2365 2723 2365
Size: 358 3736
BstAPI GCAn_nnn'nTGC
Cuts at: 1471 1471
Size: 4094
BstBI TT'CG_AA
Cuts at: 2455 2455
Size: 4094
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4094
BstYI r'GATC_y
Cuts at: 1 941 1119 1586 1609 1944 2190 3202 3288
Size: 940 178 467 23 335 246 1012 86
Cuts at: 3288 3300 3646 1
Size: 12 346 449
Fragments arranged by size:
1012 940 467 449 346 335 246 178
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 623 623
Size: 4094
BtgI C'CryG_G
Cuts at: 107 837 107
Size: 730 3364
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2044 2225 2388 2044
Size: 181 163 3750
Fragments arranged by size:
3750 181 163
BtsI GCAGTG_nn'
Cuts at: 589 1337 589
Size: 748 3346
DraI TTT'AAA
Cuts at: 1086 1093 1086
Size: 7 4087
DrdI GACnn_nn'nnGTC
Cuts at: 1800 2658 1800
Size: 858 3236
EaeI y'GGCC_r
Cuts at: 1679 1853 2244 2271 3616 1679
Size: 174 391 27 1345 2157
Fragments arranged by size:
2157 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1679 1679
Size: 4094
EarI CTCTTCn'nnn_
Cuts at: 951 2117 2327 951
Size: 1166 210 2718
Fragments arranged by size:
2718 1166 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2302 2612 2758 3904 2302
Size: 310 146 1146 2492
Fragments arranged by size:
2492 1146 310 146
EcoICRI GAG'CTC
Cuts at: 611 1106 611
Size: 495 3599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 293 1015 1046 1919 2351 3098 3366 208
Size: 85 722 31 873 432 747 268 936
Fragments arranged by size:
936 873 747 722 432 268 85 31
EcoNI CCTnn'n_nnAGG
Cuts at: 261 261
Size: 4094
EcoO109I rG'GnC_Cy
Cuts at: 844 1233 844
Size: 389 3705
EcoRI G'AATT_C
Cuts at: 1098 1098
Size: 4094
EcoRV GAT'ATC
Cuts at: 665 665
Size: 4094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 701 2113 701
Size: 1412 2682
FspI TGC'GCA
Cuts at: 1875 1875
Size: 4094
HaeII r_GCGC'y
Cuts at: 622 1528 1776 2798 3777 622
Size: 906 248 1022 979 939
Fragments arranged by size:
1022 979 939 906 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 619 651 792 824 890 922 928 960 1936
Size: 32 141 32 66 32 6 32 976
Cuts at: 1936 1966 1968 1998 619
Size: 30 2 30 2715
Fragments arranged by size:
2715 976 141 66 32 32 32 32
30 30 6 2
HincII GTy'rAC
Cuts at: 1133 1133
Size: 4094
KasI G'GCGC_C
Cuts at: 618 1524 1772 618
Size: 906 248 2940
Fragments arranged by size:
2940 906 248
KpnI G_GTAC'C
Cuts at: 1114 1114
Size: 4094
MluI A'CGCG_T
Cuts at: 3527 3527
Size: 4094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2765 2949 2765
Size: 184 3910
MscI TGG'CCA
Cuts at: 1855 1855
Size: 4094
MslI CAynn'nnrTG
Cuts at: 2083 2210 2083
Size: 127 3967
MspA1I CmG'CkG
Cuts at: 839 1205 1519 1879 2892 3137 839
Size: 366 314 360 1013 245 1796
Fragments arranged by size:
1796 1013 366 360 314 245
NaeI GCC'GGC
Cuts at: 2275 2275
Size: 4094
NarI GG'CG_CC
Cuts at: 619 1525 1773 619
Size: 906 248 2940
Fragments arranged by size:
2940 906 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4094
NgoMIV G'CCGG_C
Cuts at: 2273 2273
Size: 4094
NspI r_CATG'y
Cuts at: 1147 1647 2554 1147
Size: 500 907 2687
Fragments arranged by size:
2687 907 500
PciI A'CATG_T
Cuts at: 2550 2550
Size: 4094
PfoI T'CCnGG_A
Cuts at: 667 908 992 667
Size: 241 84 3769
Fragments arranged by size:
3769 241 84
PmeI GTTT'AAAC
Cuts at: 1093 1093
Size: 4094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 4062
PpuMI rG'GwC_Cy
Cuts at: 844 844
Size: 4094
PstI C_TGCA'G
Cuts at: 1141 1826 1141
Size: 685 3409
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4094
PvuII CAG'CTG
Cuts at: 1519 1879 1519
Size: 360 3734
RsrII CG'GwC_CG
Cuts at: 2289 2289
Size: 4094
SacI G_AGCT'C
Cuts at: 613 1108 613
Size: 495 3599
SacII CC_GC'GG
Cuts at: 840 840
Size: 4094
SalI G'TCGA_C
Cuts at: 1131 1131
Size: 4094
SanDI GG'GwC_CC
Cuts at: 844 844
Size: 4094
SapI GCTCTTCn'nnn_
Cuts at: 2117 2327 2117
Size: 210 3884
SbfI CC_TGCA'GG
Cuts at: 1141 1141
Size: 4094
ScaI AGT'ACT
Cuts at: 3611 3611
Size: 4094
SfcI C'TryA_G
Cuts at: 33 950 1137 1822 2815 3006 33
Size: 917 187 685 993 191 1121
Fragments arranged by size:
1121 993 917 685 191 187
SfoI GGC'GCC
Cuts at: 620 1526 1774 620
Size: 906 248 2940
Fragments arranged by size:
2940 906 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
SmaI CCC'GGG
Cuts at: 1116 1116
Size: 4094
SmlI C'TyrA_G
Cuts at: 1249 2164 2656 2918 1249
Size: 915 492 262 2425
Fragments arranged by size:
2425 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4094
SphI G_CATG'C
Cuts at: 1147 1647 1147
Size: 500 3594
StyI C'CwwG_G
Cuts at: 107 470 676 760 962 1075 1228 1582 107
Size: 363 206 84 202 113 153 354 2619
Fragments arranged by size:
2619 363 354 206 202 153 113 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1677 1810 2269 2478 1677
Size: 133 459 209 3293
Fragments arranged by size:
3293 459 209 133
TatI w'GTAC_w
Cuts at: 3609 3609
Size: 4094
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4062
Tth111I GACn'n_nGTC
Cuts at: 1891 1891
Size: 4094
XbaI T'CTAG_A
Cuts at: 1125 1125
Size: 4094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1589 1589
Size: 4094
XmaI C'CCGG_G
Cuts at: 1114 1114
Size: 4094
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI