Restriction map for
pF1KB6454
(Circular) (Six-base) MAPSORT of: pF1KB6454.seq Check: 4683 from: 1 to: 4163
pF1KB6454 4163 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 675 675
Size: 4163
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 542 4003 542
Size: 3461 702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 1988 2420 3167 3435 1132
Size: 856 432 747 268 1860
Fragments arranged by size:
1860 856 747 432 268
AflIII A'CryG_T
Cuts at: 1515 2619 3596 1515
Size: 1104 977 2082
Fragments arranged by size:
2082 1104 977
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 958 958
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 1132 1468 3035 1132
Size: 336 1567 2260
Fragments arranged by size:
2260 1567 336
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 535 734 1167 3516 3772 535
Size: 199 433 2349 256 926
Fragments arranged by size:
2349 926 433 256 199
AseI AT'TA_AT
Cuts at: 20 653 20
Size: 633 3530
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1183 1183
Size: 4163
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 1179 1593 1841 1876 1179
Size: 414 248 35 3466
Fragments arranged by size:
3466 414 248 35
BanII G_rGCy'C
Cuts at: 375 1177 2207 375
Size: 802 1030 2331
Fragments arranged by size:
2331 1030 802
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BbsI GAAGACnn'nnnn_
Cuts at: 733 733
Size: 4163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 137 171 137
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 864 1275 864
Size: 411 3752
Bme1580I G_kGCm'C
Cuts at: 1788 1881 2937 1788
Size: 93 1056 3014
Fragments arranged by size:
3014 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 459 766 397
Size: 62 307 3794
Fragments arranged by size:
3794 307 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 881 1339 2218 2710 3008 881
Size: 458 879 492 298 2036
Fragments arranged by size:
2036 879 492 458 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 434 1594 1842 2544 4040 434
Size: 1160 248 702 1496 557
Fragments arranged by size:
1496 1160 702 557 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 392 277
Size: 115 4048
BseYI C'CCAG_C
Cuts at: 1588 2346 2923 3419 1588
Size: 758 577 496 2332
Fragments arranged by size:
2332 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 2937 1177
Size: 778 190 792 2403
Fragments arranged by size:
2403 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 375 1177 1788 1881 1955 2145 2207 2937 375
Size: 802 611 93 74 190 62 730 1601
Fragments arranged by size:
1601 802 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 990 3339 990
Size: 2349 1814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 675 1213 1427 1729 2110 675
Size: 538 214 302 381 2728
Fragments arranged by size:
2728 538 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 2161 2342 2551 2161
Size: 181 209 3773
Fragments arranged by size:
3773 209 181
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstYI r'GATC_y
Cuts at: 1 1188 1655 1678 2013 2259 3271 3357 3369
Size: 1187 467 23 335 246 1012 86 12
Cuts at: 3369 3715 1
Size: 346 449
Fragments arranged by size:
1187 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 587 1406 587
Size: 819 3344
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 1748 1922 2313 2340 3685 1748
Size: 174 391 27 1345 2226
Fragments arranged by size:
2226 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 232 1151 2186 2396 232
Size: 919 1035 210 1999
Fragments arranged by size:
1999 1035 919 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 296 2371 2681 2827 3973 296
Size: 2075 310 146 1146 486
Fragments arranged by size:
2075 1146 486 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 459 766 1132 1988 2420 3167 3435 397
Size: 62 307 366 856 432 747 268 1125
Fragments arranged by size:
1125 856 747 432 366 307 268 62
EcoNI CCTnn'n_nnAGG
Cuts at: 247 247
Size: 4163
EcoO109I rG'GnC_Cy
Cuts at: 1140 1302 1140
Size: 162 4001
EcoRI G'AATT_C
Cuts at: 535 1167 535
Size: 632 3531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 360 1597 1845 2867 3846 360
Size: 1237 248 1022 979 677
Fragments arranged by size:
1237 1022 979 677 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 357 377 389 409 972 1004 2005
Size: 32 192 20 12 20 563 32 1001
Cuts at: 2005 2035 2037 2067 133
Size: 30 2 30 2229
Fragments arranged by size:
2229 1001 563 192 32 32 30 30
20 20 12 2
HincII GTy'rAC
Cuts at: 438 546 1202 438
Size: 108 656 3399
Fragments arranged by size:
3399 656 108
HpaI GTT'AAC
Cuts at: 546 546
Size: 4163
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 162 342 2834 3018 162
Size: 180 2492 184 1307
Fragments arranged by size:
2492 1307 184 180
MscI TGG'CCA
Cuts at: 1924 1924
Size: 4163
MslI CAynn'nnrTG
Cuts at: 2152 2279 2152
Size: 127 4036
MspA1I CmG'CkG
Cuts at: 1274 1588 1948 2961 3206 1274
Size: 314 360 1013 245 2231
Fragments arranged by size:
2231 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 107 107
Size: 4163
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 1216 1716 2623 1216
Size: 500 907 2756
Fragments arranged by size:
2756 907 500
PasI CC'CwG_GG
Cuts at: 429 429
Size: 4163
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PflMI CCAn_nnn'nTGG
Cuts at: 561 561
Size: 4163
PfoI T'CCnGG_A
Cuts at: 703 703
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PpuMI rG'GwC_Cy
Cuts at: 1140 1140
Size: 4163
PstI C_TGCA'G
Cuts at: 582 947 1210 1895 582
Size: 365 263 685 2850
Fragments arranged by size:
2850 685 365 263
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 1588 1948 1588
Size: 360 3803
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SanDI GG'GwC_CC
Cuts at: 1140 1140
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 512 578 943 1206 1891 2884 3075 33
Size: 479 66 365 263 685 993 191 1121
Fragments arranged by size:
1121 993 685 479 365 263 191 66
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 896 1318 2233 2725 2987 896
Size: 422 915 492 262 2072
Fragments arranged by size:
2072 915 492 422 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
SspI AAT'ATT
Cuts at: 1116 1116
Size: 4163
StyI C'CwwG_G
Cuts at: 107 870 1014 1297 1651 107
Size: 763 144 283 354 2619
Fragments arranged by size:
2619 763 354 283 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 568 1746 1879 2338 2547 568
Size: 1178 133 459 209 2184
Fragments arranged by size:
2184 1178 459 209 133
TatI w'GTAC_w
Cuts at: 664 3678 664
Size: 3014 1149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 176 208 3680 3712 176
Size: 32 3472 32 627
Fragments arranged by size:
3472 627 32 32
Tth111I GACn'n_nGTC
Cuts at: 568 1960 568
Size: 1392 2771
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1658 1658
Size: 4163
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI