Restriction map for
pF1KB6440
(Circular) (Six-base) MAPSORT of: pF1KB6440.seq Check: 6066 from: 1 to: 4148
pF1KB6440 4148 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 1164 1164
Size: 4148
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1973 2405 3152 3420 1973
Size: 432 747 268 2701
Fragments arranged by size:
2701 747 432 268
AflIII A'CryG_T
Cuts at: 1500 2604 3581 1500
Size: 1104 977 2067
Fragments arranged by size:
2067 1104 977
AgeI A'CCGG_T
Cuts at: 2536 2536
Size: 4148
AleI CACnn'nnGTG
Cuts at: 925 925
Size: 4148
AlwNI CAG_nnn'CTG
Cuts at: 1453 3020 1453
Size: 1567 2581
ApaLI G'TGCA_C
Cuts at: 477 2918 477
Size: 2441 1707
ApoI r'AATT_y
Cuts at: 961 1152 3501 3757 961
Size: 191 2349 256 1352
Fragments arranged by size:
2349 1352 256 191
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 135 278 1168 135
Size: 143 890 3115
Fragments arranged by size:
3115 890 143
BamHI G'GATC_C
Cuts at: 1173 1173
Size: 4148
BanI G'GyrC_C
Cuts at: 1164 1578 1826 1861 1164
Size: 414 248 35 3451
Fragments arranged by size:
3451 414 248 35
BanII G_rGCy'C
Cuts at: 502 1162 2192 502
Size: 660 1030 2458
Fragments arranged by size:
2458 1030 660
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BciVI GTATCCnnnnn_n'
Cuts at: 2046 2807 4148 2046
Size: 761 1341 2046
Fragments arranged by size:
2046 1341 761
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BfrBI ATG'CAT
Cuts at: 1123 1123
Size: 4148
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 481 1773 1866 2922 481
Size: 1292 93 1056 1707
Fragments arranged by size:
1707 1292 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 452 868 1360 1441 1776 3920 452
Size: 416 492 81 335 2144 680
Fragments arranged by size:
2144 680 492 416 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 101
Size: 4148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 2203 2695 2993 1324
Size: 879 492 298 2479
Fragments arranged by size:
2479 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3852 3852
Size: 4148
BsaAI yAC'GTr
Cuts at: 251 1501 251
Size: 1250 2898
BsaBI GATnn'nnATC
Cuts at: 6 1662 3699 6
Size: 1656 2037 455
Fragments arranged by size:
2037 1656 455
BsaHI Gr'CG_yC
Cuts at: 1579 1827 2529 4025 1579
Size: 248 702 1496 1702
Fragments arranged by size:
1702 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1337 1858 2536 2810 2957 3368 1337
Size: 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 929 959 929
Size: 30 4118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 296 296
Size: 4148
BseYI C'CCAG_C
Cuts at: 1573 2331 2908 3404 1573
Size: 758 577 496 2317
Fragments arranged by size:
2317 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 654
Size: 4148
BsiEI CG_ry'CG
Cuts at: 104 1690 1736 2944 104
Size: 1586 46 1208 1308
Fragments arranged by size:
1586 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 125 481 522 1162 1940 2130 2922 125
Size: 356 41 640 778 190 792 1351
Fragments arranged by size:
1351 792 778 640 356 190 41
BsiWI C'GTAC_G
Cuts at: 390 390
Size: 4148
BsmI GAATG_Cn'
Cuts at: 709 980 709
Size: 271 3877
Bsp1286I G_dGCh'C
Cuts at: 125 481 502 522 1162 1773 1866 1940 2130
Size: 356 21 20 640 611 93 74 190
Cuts at: 2130 2192 2922 125
Size: 62 730 1351
Fragments arranged by size:
1351 730 640 611 356 190 93 74
62 21 20
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 3324 3324
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 676 1198 1412 1714 2095 676
Size: 522 214 302 381 2729
Fragments arranged by size:
2729 522 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2440 2494 3978 4143 50
Size: 2390 54 1484 165 55
Fragments arranged by size:
2390 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2060 2060
Size: 4148
BsrFI r'CCGG_y
Cuts at: 2146 2327 2536 2146
Size: 181 209 3758
Fragments arranged by size:
3758 209 181
BsrGI T'GTAC_A
Cuts at: 343 343
Size: 4148
BssSI C'ACGA_G
Cuts at: 2419 2777 2419
Size: 358 3790
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstYI r'GATC_y
Cuts at: 1 1173 1640 1663 1998 2244 3256 3342 3354
Size: 1172 467 23 335 246 1012 86 12
Cuts at: 3354 3700 1
Size: 346 449
Fragments arranged by size:
1172 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 658 2098 2279 2442 658
Size: 1440 181 163 2364
Fragments arranged by size:
2364 1440 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 1391
Size: 4148
DraI TTT'AAA
Cuts at: 1147 1147
Size: 4148
DraIII CAC_nnn'GTG
Cuts at: 568 568
Size: 4148
DrdI GACnn_nn'nnGTC
Cuts at: 173 1854 2712 173
Size: 1681 858 1609
Fragments arranged by size:
1681 1609 858
EaeI y'GGCC_r
Cuts at: 1733 1907 2298 2325 3670 1733
Size: 174 391 27 1345 2211
Fragments arranged by size:
2211 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1733 1733
Size: 4148
EarI CTCTTCn'nnn_
Cuts at: 236 2171 2381 236
Size: 1935 210 2003
Fragments arranged by size:
2003 1935 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2356 2666 2812 3958 2356
Size: 310 146 1146 2546
Fragments arranged by size:
2546 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1160 1160
Size: 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 1973 2405 3152 3420 101
Size: 1872 432 747 268 829
Fragments arranged by size:
1872 829 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 311 311
Size: 4148
EcoO109I rG'GnC_Cy
Cuts at: 1287 1287
Size: 4148
EcoRI G'AATT_C
Cuts at: 1152 1152
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 4148
FspI TGC'GCA
Cuts at: 1929 1929
Size: 4148
HaeII r_GCGC'y
Cuts at: 464 1582 1830 2852 3831 464
Size: 1118 248 1022 979 781
Fragments arranged by size:
1118 1022 979 781 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1990 2020 2022 2052 1990
Size: 30 2 30 4086
Fragments arranged by size:
4086 30 30 2
HincII GTy'rAC
Cuts at: 1187 1187
Size: 4148
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 1168 1168
Size: 4148
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2819 3003 2819
Size: 184 3964
MscI TGG'CCA
Cuts at: 1909 1909
Size: 4148
MslI CAynn'nnrTG
Cuts at: 925 2137 2264 925
Size: 1212 127 2809
Fragments arranged by size:
2809 1212 127
MspA1I CmG'CkG
Cuts at: 586 1259 1573 1933 2946 3191 586
Size: 673 314 360 1013 245 1543
Fragments arranged by size:
1543 1013 673 360 314 245
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 4148
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 4148
NsiI A_TGCA'T
Cuts at: 1125 1125
Size: 4148
NspI r_CATG'y
Cuts at: 643 1201 1701 2608 643
Size: 558 500 907 2183
Fragments arranged by size:
2183 907 558 500
PciI A'CATG_T
Cuts at: 2604 2604
Size: 4148
PflMI CCAn_nnn'nTGG
Cuts at: 586 745 586
Size: 159 3989
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PsiI TTA'TAA
Cuts at: 575 575
Size: 4148
PstI C_TGCA'G
Cuts at: 1195 1880 1195
Size: 685 3463
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 586 1573 1933 586
Size: 987 360 2801
Fragments arranged by size:
2801 987 360
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 1162 1162
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 2171 2381 2171
Size: 210 3938
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 1191 1876 2869 3060 33
Size: 1158 685 993 191 1121
Fragments arranged by size:
1158 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 1170 1170
Size: 4148
SmlI C'TyrA_G
Cuts at: 135 1303 2218 2710 2972 135
Size: 1168 915 492 262 1311
Fragments arranged by size:
1311 1168 915 492 262
SnaBI TAC'GTA
Cuts at: 251 251
Size: 4148
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 1201 1701 1201
Size: 500 3648
StyI C'CwwG_G
Cuts at: 933 1282 1636 933
Size: 349 354 3445
Fragments arranged by size:
3445 354 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 363 1731 1864 2323 2532 363
Size: 1368 133 459 209 1979
Fragments arranged by size:
1979 1368 459 209 133
TatI w'GTAC_w
Cuts at: 343 3663 343
Size: 3320 828
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1009 1033 1041 1065 3665 3697 1009
Size: 24 8 24 2600 32 1460
Fragments arranged by size:
2600 1460 32 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 1945 1945
Size: 4148
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1643 1643
Size: 4148
XhoI C'TCGA_G
Cuts at: 135 135
Size: 4148
XmaI C'CCGG_G
Cuts at: 1168 1168
Size: 4148
XmnI GAAnn'nnTTC
Cuts at: 594 594
Size: 4148
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
ZraI