Restriction map for
pF1KB6420
(Circular) (Six-base) MAPSORT of: pF1KB6420.seq Check: 3394 from: 1 to: 4130
pF1KB6420 4130 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 165 1168 165
Size: 1003 3127
Acc65I G'GTAC_C
Cuts at: 1146 1146
Size: 4130
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 682 1955 2387 3134 3402 355
Size: 327 1273 432 747 268 1083
Fragments arranged by size:
1273 1083 747 432 327 268
AflIII A'CryG_T
Cuts at: 1482 2586 3563 1482
Size: 1104 977 2049
Fragments arranged by size:
2049 1104 977
AgeI A'CCGG_T
Cuts at: 2518 2518
Size: 4130
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 217 249 1034 1066 217
Size: 32 785 32 3281
Fragments arranged by size:
3281 785 32 32
AlwNI CAG_nnn'CTG
Cuts at: 174 1435 3002 174
Size: 1261 1567 1302
Fragments arranged by size:
1567 1302 1261
ApaLI G'TGCA_C
Cuts at: 2900 2900
Size: 4130
ApoI r'AATT_y
Cuts at: 982 1134 3483 3739 982
Size: 152 2349 256 1373
Fragments arranged by size:
2349 1373 256 152
AseI AT'TA_AT
Cuts at: 20 20
Size: 4130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 145 422 1061 1150 145
Size: 277 639 89 3125
Fragments arranged by size:
3125 639 277 89
AvrII C'CTAG_G
Cuts at: 1093 1093
Size: 4130
BamHI G'GATC_C
Cuts at: 1155 1155
Size: 4130
BanI G'GyrC_C
Cuts at: 348 1146 1560 1808 1843 348
Size: 798 414 248 35 2635
Fragments arranged by size:
2635 798 414 248 35
BanII G_rGCy'C
Cuts at: 369 423 1062 1144 2174 369
Size: 54 639 82 1030 2325
Fragments arranged by size:
2325 1030 639 82 54
BbeI G_GCGC'C
Cuts at: 1564 1812 1564
Size: 248 3882
BbvCI CC'TCA_GC
Cuts at: 116 116
Size: 4130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 403 437 403
Size: 34 4096
BciVI GTATCCnnnnn_n'
Cuts at: 2028 2789 4130 2028
Size: 761 1341 2028
Fragments arranged by size:
2028 1341 761
BclI T'GATC_A
Cuts at: 1650 1650
Size: 4130
BglII A'GATC_T
Cuts at: 1645 3682 1645
Size: 2037 2093
BlpI GC'TnA_GC
Cuts at: 998 1242 998
Size: 244 3886
Bme1580I G_kGCm'C
Cuts at: 1755 1848 2904 1755
Size: 93 1056 2981
Fragments arranged by size:
2981 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1342 1423 1758 3902 1342
Size: 81 335 2144 1570
Fragments arranged by size:
2144 1570 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 806
Size: 4130
Bpu10I CC'TnA_GC
Cuts at: 116 116
Size: 4130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 842 1306 2185 2677 2975 274
Size: 568 464 879 492 298 1429
Fragments arranged by size:
1429 879 568 492 464 298
BsaI GGTCTCn'nnnn_
Cuts at: 3834 3834
Size: 4130
BsaAI yAC'GTr
Cuts at: 1483 1483
Size: 4130
BsaBI GATnn'nnATC
Cuts at: 6 712 1644 3681 6
Size: 706 932 2037 455
Fragments arranged by size:
2037 932 706 455
BsaHI Gr'CG_yC
Cuts at: 1561 1809 2511 4007 1561
Size: 248 702 1496 1684
Fragments arranged by size:
1684 1496 702 248
BsaWI w'CCGG_w
Cuts at: 948 1319 1840 2518 2792 2939 3350 948
Size: 371 521 678 274 147 411 1728
Fragments arranged by size:
1728 678 521 411 371 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 604 634 1034 1064 217
Size: 30 357 30 400 30 3283
Fragments arranged by size:
3283 400 357 30 30 30
BseYI C'CCAG_C
Cuts at: 391 1555 2313 2890 3386 391
Size: 1164 758 577 496 1135
Fragments arranged by size:
1164 1135 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1672 1718 2926 104
Size: 1568 46 1208 1308
Fragments arranged by size:
1568 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1144 1922 2112 2904 1144
Size: 778 190 792 2370
Fragments arranged by size:
2370 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 369 423 1062 1144 1755 1848 1922 2112 2174
Size: 54 639 82 611 93 74 190 62
Cuts at: 2174 2904 369
Size: 730 1595
Fragments arranged by size:
1595 730 639 611 190 93 82 74
62 54
BspEI T'CCGG_A
Cuts at: 3350 3350
Size: 4130
BspHI T'CATG_A
Cuts at: 3306 3306
Size: 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1180 1394 1696 2077 1180
Size: 214 302 381 3233
Fragments arranged by size:
3233 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2422 2476 3960 4125 50
Size: 2372 54 1484 165 55
Fragments arranged by size:
2372 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 456 635 2042 456
Size: 179 1407 2544
Fragments arranged by size:
2544 1407 179
BsrFI r'CCGG_y
Cuts at: 205 2128 2309 2518 205
Size: 1923 181 209 1817
Fragments arranged by size:
1923 1817 209 181
BssSI C'ACGA_G
Cuts at: 2401 2759 2401
Size: 358 3772
BstAPI GCAn_nnn'nTGC
Cuts at: 1507 1507
Size: 4130
BstBI TT'CG_AA
Cuts at: 155 2491 155
Size: 2336 1794
BstYI r'GATC_y
Cuts at: 1 755 1155 1622 1645 1980 2226 3238 3324
Size: 754 400 467 23 335 246 1012 86
Cuts at: 3324 3336 3682 1
Size: 12 346 449
Fragments arranged by size:
1012 754 467 449 400 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 136 533 107
Size: 29 397 3704
Fragments arranged by size:
3704 397 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2080 2261 2424 2080
Size: 181 163 3786
Fragments arranged by size:
3786 181 163
BtsI GCAGTG_nn'
Cuts at: 518 1373 518
Size: 855 3275
DraI TTT'AAA
Cuts at: 1129 1129
Size: 4130
DrdI GACnn_nn'nnGTC
Cuts at: 1836 2694 1836
Size: 858 3272
EaeI y'GGCC_r
Cuts at: 1715 1889 2280 2307 3652 1715
Size: 174 391 27 1345 2193
Fragments arranged by size:
2193 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1715 1715
Size: 4130
EarI CTCTTCn'nnn_
Cuts at: 308 719 780 1025 2153 2363 308
Size: 411 61 245 1128 210 2075
Fragments arranged by size:
2075 1128 411 245 210 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2338 2648 2794 3940 2338
Size: 310 146 1146 2528
Fragments arranged by size:
2528 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1142 1142
Size: 4130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 682 806 1955 2387 3134 3402 355
Size: 327 124 1149 432 747 268 1083
Fragments arranged by size:
1149 1083 747 432 327 268 124
EcoNI CCTnn'n_nnAGG
Cuts at: 256 256
Size: 4130
EcoO109I rG'GnC_Cy
Cuts at: 1269 1269
Size: 4130
EcoRI G'AATT_C
Cuts at: 982 1134 982
Size: 152 3978
EcoRV GAT'ATC
Cuts at: 714 714
Size: 4130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 791 2149 791
Size: 1358 2772
FspI TGC'GCA
Cuts at: 1911 1911
Size: 4130
HaeII r_GCGC'y
Cuts at: 1564 1812 2834 3813 1564
Size: 248 1022 979 1881
Fragments arranged by size:
1881 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1972 2002 2004 2034 1972
Size: 30 2 30 4068
Fragments arranged by size:
4068 30 30 2
HincII GTy'rAC
Cuts at: 387 1169 387
Size: 782 3348
KasI G'GCGC_C
Cuts at: 1560 1808 1560
Size: 248 3882
KpnI G_GTAC'C
Cuts at: 1150 1150
Size: 4130
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 2801 2985 590
Size: 2211 184 1735
Fragments arranged by size:
2211 1735 184
MscI TGG'CCA
Cuts at: 1891 1891
Size: 4130
MslI CAynn'nnrTG
Cuts at: 2119 2246 2119
Size: 127 4003
MspA1I CmG'CkG
Cuts at: 132 138 417 1241 1555 1915 2928 3173 132
Size: 6 279 824 314 360 1013 245 1089
Fragments arranged by size:
1089 1013 824 360 314 279 245 6
NaeI GCC'GGC
Cuts at: 2311 2311
Size: 4130
NarI GG'CG_CC
Cuts at: 1561 1809 1561
Size: 248 3882
NcoI C'CATG_G
Cuts at: 107 533 107
Size: 426 3704
NgoMIV G'CCGG_C
Cuts at: 2309 2309
Size: 4130
NspI r_CATG'y
Cuts at: 1183 1683 2590 1183
Size: 500 907 2723
Fragments arranged by size:
2723 907 500
PciI A'CATG_T
Cuts at: 2586 2586
Size: 4130
PfoI T'CCnGG_A
Cuts at: 211 221 211
Size: 10 4120
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 217 249 1034 1066 3269 3301 217
Size: 32 785 32 2203 32 1046
Fragments arranged by size:
2203 1046 785 32 32 32
PsiI TTA'TAA
Cuts at: 436 436
Size: 4130
PstI C_TGCA'G
Cuts at: 1177 1862 1177
Size: 685 3445
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 132 1555 1915 132
Size: 1423 360 2347
Fragments arranged by size:
2347 1423 360
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 1144 1144
Size: 4130
SacII CC_GC'GG
Cuts at: 139 139
Size: 4130
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 2153 2363 2153
Size: 210 3920
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 3647 3647
Size: 4130
SfcI C'TryA_G
Cuts at: 33 1173 1858 2851 3042 33
Size: 1140 685 993 191 1121
Fragments arranged by size:
1140 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1562 1810 1562
Size: 248 3882
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SmaI CCC'GGG
Cuts at: 1063 1152 1063
Size: 89 4041
SmlI C'TyrA_G
Cuts at: 253 422 857 1285 2200 2692 2954 253
Size: 169 435 428 915 492 262 1429
Fragments arranged by size:
1429 915 492 435 428 262 169
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4130
SphI G_CATG'C
Cuts at: 1183 1683 1183
Size: 500 3630
SspI AAT'ATT
Cuts at: 682 682
Size: 4130
StuI AGG'CCT
Cuts at: 819 819
Size: 4130
StyI C'CwwG_G
Cuts at: 107 533 965 1093 1264 1618 107
Size: 426 432 128 171 354 2619
Fragments arranged by size:
2619 432 426 354 171 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1102 1713 1846 2305 2514 1102
Size: 611 133 459 209 2718
Fragments arranged by size:
2718 611 459 209 133
TatI w'GTAC_w
Cuts at: 3645 3645
Size: 4130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4098
Tth111I GACn'n_nGTC
Cuts at: 1927 1927
Size: 4130
XbaI T'CTAG_A
Cuts at: 402 1161 402
Size: 759 3371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 531 1625 531
Size: 1094 3036
XhoI C'TCGA_G
Cuts at: 422 422
Size: 4130
XmaI C'CCGG_G
Cuts at: 1061 1150 1061
Size: 89 4041
XmnI GAAnn'nnTTC
Cuts at: 232 832 232
Size: 600 3530
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI BaeI BbsI BfrBI BglI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI