Restriction map for
pF1KB6417
(Circular) (Six-base) MAPSORT of: pF1KB6417.seq Check: 8130 from: 1 to: 4040
pF1KB6417 4040 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 548 1078 548
Size: 530 3510
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1865 2297 3044 3312 733
Size: 1132 432 747 268 1461
Fragments arranged by size:
1461 1132 747 432 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 859 1044 3393 3649 859
Size: 185 2349 256 1250
Fragments arranged by size:
2349 1250 256 185
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 415 1060 415
Size: 645 3395
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 236 394 748 791 883 1056 1470 1718 1753
Size: 158 354 43 92 173 414 248 35
Cuts at: 1753 236
Size: 2523
Fragments arranged by size:
2523 414 354 248 173 158 92 43
35
BanII G_rGCy'C
Cuts at: 779 1054 2084 779
Size: 275 1030 2735
Fragments arranged by size:
2735 1030 275
BbeI G_GCGC'C
Cuts at: 240 398 1474 1722 240
Size: 158 1076 248 2558
Fragments arranged by size:
2558 1076 248 158
BbvCI CC'TCA_GC
Cuts at: 125 125
Size: 4040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4006
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 888 1665 1758 2814 888
Size: 777 93 1056 2114
Fragments arranged by size:
2114 1056 777 93
BmgBI CAC'GTC
Cuts at: 447 447
Size: 4040
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 358 475 863 116
Size: 242 117 388 3293
Fragments arranged by size:
3293 388 242 117
Bpu10I CC'TnA_GC
Cuts at: 125 620 125
Size: 495 3545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 192 3744 192
Size: 3552 488
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 907 1554 3591 6
Size: 901 647 2037 455
Fragments arranged by size:
2037 901 647 455
BsaHI Gr'CG_yC
Cuts at: 237 374 387 395 1471 1719 2421 3917 237
Size: 137 13 8 1076 248 702 1496 360
Fragments arranged by size:
1496 1076 702 360 248 137 13 8
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 180 316 490 529 180
Size: 136 174 39 3691
Fragments arranged by size:
3691 174 136 39
BseYI C'CCAG_C
Cuts at: 432 459 466 652 940 1465 2223 2800 3296
Size: 27 7 186 288 525 758 577 496
Cuts at: 3296 432
Size: 1176
Fragments arranged by size:
1176 758 577 525 496 288 186 27
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 969 691
Size: 278 3762
BsiEI CG_ry'CG
Cuts at: 104 120 281 1582 1628 2836 104
Size: 16 161 1301 46 1208 1308
Fragments arranged by size:
1308 1301 1208 161 46 16
BsiHKAI G_wGCw'C
Cuts at: 779 1054 1832 2022 2814 779
Size: 275 778 190 792 2005
Fragments arranged by size:
2005 792 778 275 190
Bsp1286I G_dGCh'C
Cuts at: 779 888 1054 1665 1758 1832 2022 2084 2814
Size: 109 166 611 93 74 190 62 730
Cuts at: 2814 779
Size: 2005
Fragments arranged by size:
2005 730 611 190 166 109 93 74
62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 836 1090 1304 1606 1987 836
Size: 254 214 302 381 2889
Fragments arranged by size:
2889 381 302 254 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 929 1952 929
Size: 1023 3017
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 326 1065 1532 1555 1890 2136 3148 3234
Size: 325 739 467 23 335 246 1012 86
Cuts at: 3234 3246 3592 1
Size: 12 346 449
Fragments arranged by size:
1012 739 467 449 346 335 325 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 949 949
Size: 4040
BtgI C'CryG_G
Cuts at: 146 562 146
Size: 416 3624
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 749 1990 2171 2334 749
Size: 1241 181 163 2455
Fragments arranged by size:
2455 1241 181 163
BtsI GCAGTG_nn'
Cuts at: 561 1283 561
Size: 722 3318
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 556 736 1746 2604 556
Size: 180 1010 858 1992
Fragments arranged by size:
1992 1010 858 180
EaeI y'GGCC_r
Cuts at: 278 539 1625 1799 2190 2217 3562 278
Size: 261 1086 174 391 27 1345 756
Fragments arranged by size:
1345 1086 756 391 261 174 27
EagI C'GGCC_G
Cuts at: 278 1625 278
Size: 1347 2693
EarI CTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 313 2248 2558 2704 3850 313
Size: 1935 310 146 1146 503
Fragments arranged by size:
1935 1146 503 310 146
EcoICRI GAG'CTC
Cuts at: 777 1052 777
Size: 275 3765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 358 475 733 863 1865 2297 3044 3312
Size: 242 117 258 130 1002 432 747 268
Cuts at: 3312 116
Size: 844
Fragments arranged by size:
1002 844 747 432 268 258 242 130
117
EcoNI CCTnn'n_nnAGG
Cuts at: 1015 1015
Size: 4040
EcoO109I rG'GnC_Cy
Cuts at: 160 463 1179 160
Size: 303 716 3021
Fragments arranged by size:
3021 716 303
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 513 2059 513
Size: 1546 2494
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 240 398 1474 1722 2744 3723 240
Size: 158 1076 248 1022 979 557
Fragments arranged by size:
1076 1022 979 557 248 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 690 722 1882 1912 1914 1944 690
Size: 32 1160 30 2 30 2786
Fragments arranged by size:
2786 1160 32 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 236 394 1470 1718 236
Size: 158 1076 248 2558
Fragments arranged by size:
2558 1076 248 158
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 229 2029 2156 229
Size: 1800 127 2113
Fragments arranged by size:
2113 1800 127
MspA1I CmG'CkG
Cuts at: 544 1151 1465 1825 2838 3083 544
Size: 607 314 360 1013 245 1501
Fragments arranged by size:
1501 1013 607 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 237 395 1471 1719 237
Size: 158 1076 248 2558
Fragments arranged by size:
2558 1076 248 158
NcoI C'CATG_G
Cuts at: 146 146
Size: 4040
NdeI CA'TA_TG
Cuts at: 614 614
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 228 1093 1593 2500 228
Size: 865 500 907 1768
Fragments arranged by size:
1768 907 865 500
PasI CC'CwG_GG
Cuts at: 606 606
Size: 4040
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 995 995
Size: 4040
PfoI T'CCnGG_A
Cuts at: 132 132
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PpuMI rG'GwC_Cy
Cuts at: 160 463 160
Size: 303 3737
PspXI vC'TCGA_Gb
Cuts at: 415 415
Size: 4040
PstI C_TGCA'G
Cuts at: 156 833 1087 1772 156
Size: 677 254 685 2424
Fragments arranged by size:
2424 685 677 254
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 1465 1825 1465
Size: 360 3680
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 779 1054 779
Size: 275 3765
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SanDI GG'GwC_CC
Cuts at: 463 463
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 319 3557 319
Size: 3238 802
SfcI C'TryA_G
Cuts at: 33 152 401 545 829 1083 1768 2761 2952
Size: 119 249 144 284 254 685 993 191
Cuts at: 2952 33
Size: 1121
Fragments arranged by size:
1121 993 685 284 254 249 191 144
119
SfoI GGC'GCC
Cuts at: 238 396 1472 1720 238
Size: 158 1076 248 2558
Fragments arranged by size:
2558 1076 248 158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 415 1195 2110 2602 2864 415
Size: 780 915 492 262 1591
Fragments arranged by size:
1591 915 780 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StuI AGG'CCT
Cuts at: 1021 1021
Size: 4040
StyI C'CwwG_G
Cuts at: 146 1016 1174 1528 146
Size: 870 158 354 2658
Fragments arranged by size:
2658 870 354 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 317 914 3555 317
Size: 597 2641 802
Fragments arranged by size:
2641 802 597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XhoI C'TCGA_G
Cuts at: 415 415
Size: 4040
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
XmnI GAAnn'nnTTC
Cuts at: 934 934
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BfrBI BglI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspOMI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI