Restriction map for
pF1KB6410
(Circular) (Six-base) MAPSORT of: pF1KB6410.seq Check: 8627 from: 1 to: 4118
pF1KB6410 4118 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 507 507
Size: 4118
AatII G_ACGT'C
Cuts at: 713 713
Size: 4118
AccI GT'mk_AC
Cuts at: 1156 1156
Size: 4118
Acc65I G'GTAC_C
Cuts at: 1134 1134
Size: 4118
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1943 2375 3122 3390 631
Size: 1312 432 747 268 1359
Fragments arranged by size:
1359 1312 747 432 268
AflIII A'CryG_T
Cuts at: 1470 2574 3551 1470
Size: 1104 977 2037
Fragments arranged by size:
2037 1104 977
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 539 951 539
Size: 412 3706
AlwNI CAG_nnn'CTG
Cuts at: 298 1423 2990 298
Size: 1125 1567 1426
Fragments arranged by size:
1567 1426 1125
ApaI G_GGCC'C
Cuts at: 346 346
Size: 4118
ApaLI G'TGCA_C
Cuts at: 2888 2888
Size: 4118
ApoI r'AATT_y
Cuts at: 1122 3471 3727 1122
Size: 2349 256 1513
Fragments arranged by size:
2349 1513 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 602 1138 602
Size: 536 3582
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1060 1093 1060
Size: 33 4085
BamHI G'GATC_C
Cuts at: 725 1143 725
Size: 418 3700
BanI G'GyrC_C
Cuts at: 355 1134 1548 1796 1831 355
Size: 779 414 248 35 2642
Fragments arranged by size:
2642 779 414 248 35
BanII G_rGCy'C
Cuts at: 346 954 1132 2162 346
Size: 608 178 1030 2302
Fragments arranged by size:
2302 1030 608 178
BbeI G_GCGC'C
Cuts at: 1552 1800 1552
Size: 248 3870
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 310 344 90
Size: 34 186 34 3864
Fragments arranged by size:
3864 186 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 631 2016 2777 4118 631
Size: 1385 761 1341 631
Fragments arranged by size:
1385 1341 761 631
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4118
BglII A'GATC_T
Cuts at: 1633 3670 1633
Size: 2037 2081
BlpI GC'TnA_GC
Cuts at: 1230 1230
Size: 4118
Bme1580I G_kGCm'C
Cuts at: 346 360 1743 1836 2892 346
Size: 14 1383 93 1056 1572
Fragments arranged by size:
1572 1383 1056 93 14
BmrI ACTGGGnnnn_n'
Cuts at: 1330 1411 1746 3890 1330
Size: 81 335 2144 1558
Fragments arranged by size:
2144 1558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 4118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1125 491
Size: 634 3484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1294 2173 2665 2963 1294
Size: 879 492 298 2449
Fragments arranged by size:
2449 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3822 3822
Size: 4118
BsaAI yAC'GTr
Cuts at: 1471 1471
Size: 4118
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 710 1549 1797 2499 3995 710
Size: 839 248 702 1496 833
Fragments arranged by size:
1496 839 833 702 248
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2780 2927 3338 1307
Size: 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 905 905
Size: 4118
BseYI C'CCAG_C
Cuts at: 120 398 1016 1543 2301 2878 3374 120
Size: 278 618 527 758 577 496 864
Fragments arranged by size:
864 758 618 577 527 496 278
BsiEI CG_ry'CG
Cuts at: 104 1660 1706 2914 104
Size: 1556 46 1208 1308
Fragments arranged by size:
1556 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1132 1910 2100 2892 1132
Size: 778 190 792 2358
Fragments arranged by size:
2358 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 717 717
Size: 4118
Bsp1286I G_dGCh'C
Cuts at: 346 360 954 1132 1743 1836 1910 2100 2162
Size: 14 594 178 611 93 74 190 62
Cuts at: 2162 2892 346
Size: 730 1572
Fragments arranged by size:
1572 730 611 594 190 178 93 74
62 14
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 3294 3294
Size: 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 281 507 1168 1382 1684 2065 281
Size: 226 661 214 302 381 2334
Fragments arranged by size:
2334 661 381 302 226 214
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 326 830 2030 326
Size: 504 1200 2414
Fragments arranged by size:
2414 1200 504
BsrFI r'CCGG_y
Cuts at: 111 2116 2297 2506 111
Size: 2005 181 209 1723
Fragments arranged by size:
2005 1723 209 181
BssSI C'ACGA_G
Cuts at: 2389 2747 2389
Size: 358 3760
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstEII G'GTnAC_C
Cuts at: 1098 1098
Size: 4118
BstXI CCAn_nnnn'nTGG
Cuts at: 946 946
Size: 4118
BstYI r'GATC_y
Cuts at: 1 725 789 1143 1610 1633 1968 2214 3226
Size: 724 64 354 467 23 335 246 1012
Cuts at: 3226 3312 3324 3670 1
Size: 86 12 346 449
Fragments arranged by size:
1012 724 467 449 354 346 335 246
86 64 23 12
Bsu36I CC'TnA_GG
Cuts at: 785 785
Size: 4118
BtgI C'CryG_G
Cuts at: 190 851 190
Size: 661 3457
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1012 2068 2249 2412 1012
Size: 1056 181 163 2718
Fragments arranged by size:
2718 1056 181 163
BtsI GCAGTG_nn'
Cuts at: 581 1361 581
Size: 780 3338
ClaI AT'CG_AT
Cuts at: 566 566
Size: 4118
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DraIII CAC_nnn'GTG
Cuts at: 862 1054 862
Size: 192 3926
DrdI GACnn_nn'nnGTC
Cuts at: 1824 2682 1824
Size: 858 3260
EaeI y'GGCC_r
Cuts at: 764 1703 1877 2268 2295 3640 764
Size: 939 174 391 27 1345 1242
Fragments arranged by size:
1345 1242 939 391 174 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 210 2326 2636 2782 3928 210
Size: 2116 310 146 1146 400
Fragments arranged by size:
2116 1146 400 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 631 1125 1943 2375 3122 3390 491
Size: 140 494 818 432 747 268 1219
Fragments arranged by size:
1219 818 747 494 432 268 140
EcoNI CCTnn'n_nnAGG
Cuts at: 218 277 218
Size: 59 4059
EcoO109I rG'GnC_Cy
Cuts at: 342 343 876 1043 1257 342
Size: 1 533 167 214 3203
Fragments arranged by size:
3203 533 214 167 1
EcoRI G'AATT_C
Cuts at: 1122 1122
Size: 4118
EcoRV GAT'ATC
Cuts at: 318 318
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 4118
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 140 1552 1800 2822 3801 140
Size: 1412 248 1022 979 457
Fragments arranged by size:
1412 1022 979 457 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 248 280 1960 1990 1992 2022 248
Size: 32 1680 30 2 30 2344
Fragments arranged by size:
2344 1680 32 30 30 2
HincII GTy'rAC
Cuts at: 1157 1157
Size: 4118
KasI G'GCGC_C
Cuts at: 1548 1796 1548
Size: 248 3870
KpnI G_GTAC'C
Cuts at: 1138 1138
Size: 4118
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2789 2973 2789
Size: 184 3934
MscI TGG'CCA
Cuts at: 766 1879 766
Size: 1113 3005
MslI CAynn'nnrTG
Cuts at: 451 2107 2234 451
Size: 1656 127 2335
Fragments arranged by size:
2335 1656 127
MspA1I CmG'CkG
Cuts at: 192 402 1229 1543 1903 2916 3161 192
Size: 210 827 314 360 1013 245 1149
Fragments arranged by size:
1149 1013 827 360 314 245 210
NaeI GCC'GGC
Cuts at: 113 2299 113
Size: 2186 1932
NarI GG'CG_CC
Cuts at: 1549 1797 1549
Size: 248 3870
NcoI C'CATG_G
Cuts at: 851 851
Size: 4118
NgoMIV G'CCGG_C
Cuts at: 111 2297 111
Size: 2186 1932
NspI r_CATG'y
Cuts at: 1171 1671 2578 1171
Size: 500 907 2711
Fragments arranged by size:
2711 907 500
PciI A'CATG_T
Cuts at: 2574 2574
Size: 4118
PfoI T'CCnGG_A
Cuts at: 633 633
Size: 4118
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 4086
PpuMI rG'GwC_Cy
Cuts at: 1043 1043
Size: 4118
PshAI GACnn'nnGTC
Cuts at: 202 629 202
Size: 427 3691
PspOMI G'GGCC_C
Cuts at: 342 342
Size: 4118
PspXI vC'TCGA_Gb
Cuts at: 602 602
Size: 4118
PstI C_TGCA'G
Cuts at: 281 809 1165 1850 281
Size: 528 356 685 2549
Fragments arranged by size:
2549 685 528 356
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 402 1543 1903 402
Size: 1141 360 2617
Fragments arranged by size:
2617 1141 360
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SacII CC_GC'GG
Cuts at: 193 193
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
SbfI CC_TGCA'GG
Cuts at: 809 1165 809
Size: 356 3762
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SfcI C'TryA_G
Cuts at: 33 277 805 913 965 1161 1846 2839 3030
Size: 244 528 108 52 196 685 993 191
Cuts at: 3030 33
Size: 1121
Fragments arranged by size:
1121 993 685 528 244 196 191 108
52
SfoI GGC'GCC
Cuts at: 1550 1798 1550
Size: 248 3870
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 1140 1140
Size: 4118
SmlI C'TyrA_G
Cuts at: 602 1273 2188 2680 2942 602
Size: 671 915 492 262 1778
Fragments arranged by size:
1778 915 671 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 1171 1671 1171
Size: 500 3618
StyI C'CwwG_G
Cuts at: 338 851 1252 1606 338
Size: 513 401 354 2850
Fragments arranged by size:
2850 513 401 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 343 412 1701 1834 2293 2502 343
Size: 69 1289 133 459 209 1959
Fragments arranged by size:
1959 1289 459 209 133 69
TatI w'GTAC_w
Cuts at: 778 3633 778
Size: 2855 1263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4086
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1613 1613
Size: 4118
XhoI C'TCGA_G
Cuts at: 602 602
Size: 4118
XmaI C'CCGG_G
Cuts at: 1138 1138
Size: 4118
ZraI GAC'GTC
Cuts at: 711 711
Size: 4118
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BbsI
BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI
BsmI BsrGI BssHII BstZ17I CspCI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PsiI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI