Restriction map for
pF1KB6394
(Circular) (Six-base) MAPSORT of: pF1KB6394.seq Check: 2688 from: 1 to: 4022
pF1KB6394 4022 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 625 625
Size: 4022
AccI GT'mk_AC
Cuts at: 1060 1060
Size: 4022
Acc65I G'GTAC_C
Cuts at: 508 927 1038 508
Size: 419 111 3492
Fragments arranged by size:
3492 419 111
AclI AA'CG_TT
Cuts at: 3862 3862
Size: 4022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1847 2279 3026 3294 1847
Size: 432 747 268 2575
Fragments arranged by size:
2575 747 432 268
AflIII A'CryG_T
Cuts at: 518 1374 2478 3455 518
Size: 856 1104 977 1085
Fragments arranged by size:
1104 1085 977 856
AgeI A'CCGG_T
Cuts at: 2410 2410
Size: 4022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 733 765 733
Size: 32 3990
AlwNI CAG_nnn'CTG
Cuts at: 542 1327 2894 542
Size: 785 1567 1670
Fragments arranged by size:
1670 1567 785
ApaLI G'TGCA_C
Cuts at: 2792 2792
Size: 4022
ApoI r'AATT_y
Cuts at: 1026 3375 3631 1026
Size: 2349 256 1417
Fragments arranged by size:
2349 1417 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4022
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
AvaI C'yCGr_G
Cuts at: 1042 1042
Size: 4022
BamHI G'GATC_C
Cuts at: 1047 1047
Size: 4022
BanI G'GyrC_C
Cuts at: 141 508 927 1038 1452 1700 1735 141
Size: 367 419 111 414 248 35 2428
Fragments arranged by size:
2428 419 414 367 248 111 35
BanII G_rGCy'C
Cuts at: 221 1036 2066 221
Size: 815 1030 2177
Fragments arranged by size:
2177 1030 815
BbeI G_GCGC'C
Cuts at: 145 1456 1704 145
Size: 1311 248 2463
Fragments arranged by size:
2463 1311 248
BciVI GTATCCnnnnn_n'
Cuts at: 1920 2681 4022 1920
Size: 761 1341 1920
Fragments arranged by size:
1920 1341 761
BclI T'GATC_A
Cuts at: 1542 1542
Size: 4022
BglI GCCn_nnn'nGGC
Cuts at: 118 118
Size: 4022
BglII A'GATC_T
Cuts at: 1537 3574 1537
Size: 2037 1985
BlpI GC'TnA_GC
Cuts at: 1010 1134 1010
Size: 124 3898
Bme1580I G_kGCm'C
Cuts at: 1647 1740 2796 1647
Size: 93 1056 2873
Fragments arranged by size:
2873 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1234 1315 1650 3794 1234
Size: 81 335 2144 1462
Fragments arranged by size:
2144 1462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 840 843 2389 840
Size: 3 1546 2473
Fragments arranged by size:
2473 1546 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 641 731 244
Size: 397 90 3535
Fragments arranged by size:
3535 397 90
Bpu10I CC'TnA_GC
Cuts at: 122 122
Size: 4022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1198 2077 2569 2867 511
Size: 687 879 492 298 1666
Fragments arranged by size:
1666 879 687 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 487 828 985 3726 487
Size: 341 157 2741 783
Fragments arranged by size:
2741 783 341 157
BsaAI yAC'GTr
Cuts at: 1375 1375
Size: 4022
BsaBI GATnn'nnATC
Cuts at: 6 1536 3573 6
Size: 1530 2037 455
Fragments arranged by size:
2037 1530 455
BsaHI Gr'CG_yC
Cuts at: 142 1453 1701 2403 3899 142
Size: 1311 248 702 1496 265
Fragments arranged by size:
1496 1311 702 265 248
BsaWI w'CCGG_w
Cuts at: 350 1211 1732 2410 2684 2831 3242 350
Size: 861 521 678 274 147 411 1130
Fragments arranged by size:
1130 861 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 376 446 816 832 373
Size: 3 70 370 16 3563
Fragments arranged by size:
3563 370 70 16 3
BseYI C'CCAG_C
Cuts at: 228 1447 2205 2782 3278 228
Size: 1219 758 577 496 972
Fragments arranged by size:
1219 972 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1564 1610 2818 104
Size: 1460 46 1208 1308
Fragments arranged by size:
1460 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 221 1036 1814 2004 2796 221
Size: 815 778 190 792 1447
Fragments arranged by size:
1447 815 792 778 190
BsmI GAATG_Cn'
Cuts at: 615 615
Size: 4022
Bsp1286I G_dGCh'C
Cuts at: 221 1036 1647 1740 1814 2004 2066 2796 221
Size: 815 611 93 74 190 62 730 1447
Fragments arranged by size:
1447 815 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 350 3242 350
Size: 2892 1130
BspHI T'CATG_A
Cuts at: 936 3198 936
Size: 2262 1760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 625 1072 1286 1588 1969 625
Size: 447 214 302 381 2678
Fragments arranged by size:
2678 447 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2314 2368 3852 4017 50
Size: 2264 54 1484 165 55
Fragments arranged by size:
2264 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 245 1934 245
Size: 1689 2333
BsrFI r'CCGG_y
Cuts at: 178 2020 2201 2410 178
Size: 1842 181 209 1790
Fragments arranged by size:
1842 1790 209 181
BssSI C'ACGA_G
Cuts at: 323 2293 2651 323
Size: 1970 358 1694
Fragments arranged by size:
1970 1694 358
BstAPI GCAn_nnn'nTGC
Cuts at: 486 1399 486
Size: 913 3109
BstBI TT'CG_AA
Cuts at: 2383 2383
Size: 4022
BstYI r'GATC_y
Cuts at: 1 562 1047 1514 1537 1872 2118 3130 3216
Size: 561 485 467 23 335 246 1012 86
Cuts at: 3216 3228 3574 1
Size: 12 346 449
Fragments arranged by size:
1012 561 485 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 815 815
Size: 4022
BtgI C'CryG_G
Cuts at: 107 188 107
Size: 81 3941
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1972 2153 2316 1972
Size: 181 163 3678
Fragments arranged by size:
3678 181 163
BtsI GCAGTG_nn'
Cuts at: 687 1265 687
Size: 578 3444
DraI TTT'AAA
Cuts at: 1021 1021
Size: 4022
DraIII CAC_nnn'GTG
Cuts at: 486 486
Size: 4022
DrdI GACnn_nn'nnGTC
Cuts at: 1728 2586 1728
Size: 858 3164
EaeI y'GGCC_r
Cuts at: 167 1607 1781 2172 2199 3544 167
Size: 1440 174 391 27 1345 645
Fragments arranged by size:
1440 1345 645 391 174 27
EagI C'GGCC_G
Cuts at: 1607 1607
Size: 4022
EarI CTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2230 2540 2686 3832 2230
Size: 310 146 1146 2420
Fragments arranged by size:
2420 1146 310 146
EcoICRI GAG'CTC
Cuts at: 219 1034 219
Size: 815 3207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 641 731 1847 2279 3026 3294 244
Size: 397 90 1116 432 747 268 972
Fragments arranged by size:
1116 972 747 432 397 268 90
EcoO109I rG'GnC_Cy
Cuts at: 963 1161 963
Size: 198 3824
EcoRI G'AATT_C
Cuts at: 1026 1026
Size: 4022
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1011 2041 1011
Size: 1030 2992
FspI TGC'GCA
Cuts at: 1803 1803
Size: 4022
HaeII r_GCGC'y
Cuts at: 145 1456 1704 2726 3705 145
Size: 1311 248 1022 979 462
Fragments arranged by size:
1311 1022 979 462 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 551 554 583 586 1864 1894 1896 1926 551
Size: 3 29 3 1278 30 2 30 2647
Fragments arranged by size:
2647 1278 30 30 29 3 3 2
HincII GTy'rAC
Cuts at: 1061 1061
Size: 4022
HindIII A'AGCT_T
Cuts at: 762 762
Size: 4022
KasI G'GCGC_C
Cuts at: 141 1452 1700 141
Size: 1311 248 2463
Fragments arranged by size:
2463 1311 248
KpnI G_GTAC'C
Cuts at: 512 931 1042 512
Size: 419 111 3492
Fragments arranged by size:
3492 419 111
MfeI C'AATT_G
Cuts at: 696 696
Size: 4022
MluI A'CGCG_T
Cuts at: 3455 3455
Size: 4022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 242 2693 2877 242
Size: 2451 184 1387
Fragments arranged by size:
2451 1387 184
MscI TGG'CCA
Cuts at: 169 1783 169
Size: 1614 2408
MslI CAynn'nnrTG
Cuts at: 920 2011 2138 920
Size: 1091 127 2804
Fragments arranged by size:
2804 1091 127
MspA1I CmG'CkG
Cuts at: 1133 1447 1807 2820 3065 1133
Size: 314 360 1013 245 2090
Fragments arranged by size:
2090 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2203 2203
Size: 4022
NarI GG'CG_CC
Cuts at: 142 1453 1701 142
Size: 1311 248 2463
Fragments arranged by size:
2463 1311 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4022
NgoMIV G'CCGG_C
Cuts at: 2201 2201
Size: 4022
NspI r_CATG'y
Cuts at: 522 1075 1575 2482 522
Size: 553 500 907 2062
Fragments arranged by size:
2062 907 553 500
PciI A'CATG_T
Cuts at: 518 2478 518
Size: 1960 2062
PfoI T'CCnGG_A
Cuts at: 221 541 221
Size: 320 3702
PmeI GTTT'AAAC
Cuts at: 1021 1021
Size: 4022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3990
PpuMI rG'GwC_Cy
Cuts at: 963 963
Size: 4022
PstI C_TGCA'G
Cuts at: 158 296 682 858 1069 1754 158
Size: 138 386 176 211 685 2426
Fragments arranged by size:
2426 685 386 211 176 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4022
PvuII CAG'CTG
Cuts at: 1447 1807 1447
Size: 360 3662
RsrII CG'GwC_CG
Cuts at: 2217 2217
Size: 4022
SacI G_AGCT'C
Cuts at: 221 1036 221
Size: 815 3207
SalI G'TCGA_C
Cuts at: 1059 1059
Size: 4022
SapI GCTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
SbfI CC_TGCA'GG
Cuts at: 1069 1069
Size: 4022
ScaI AGT'ACT
Cuts at: 3539 3539
Size: 4022
SfcI C'TryA_G
Cuts at: 33 154 292 678 854 1065 1750 2743 2934
Size: 121 138 386 176 211 685 993 191
Cuts at: 2934 33
Size: 1121
Fragments arranged by size:
1121 993 685 386 211 191 176 138
121
SfoI GGC'GCC
Cuts at: 143 1454 1702 143
Size: 1311 248 2463
Fragments arranged by size:
2463 1311 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
SgrAI Cr'CCGG_yG
Cuts at: 178 178
Size: 4022
SmaI CCC'GGG
Cuts at: 1044 1044
Size: 4022
SmlI C'TyrA_G
Cuts at: 490 1177 2092 2584 2846 490
Size: 687 915 492 262 1666
Fragments arranged by size:
1666 915 687 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4022
SphI G_CATG'C
Cuts at: 1075 1575 1075
Size: 500 3522
StyI C'CwwG_G
Cuts at: 107 950 1156 1510 107
Size: 843 206 354 2619
Fragments arranged by size:
2619 843 354 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1605 1738 2197 2406 1605
Size: 133 459 209 3221
Fragments arranged by size:
3221 459 209 133
TatI w'GTAC_w
Cuts at: 3537 3537
Size: 4022
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 684 716 866 898 3539 3571 684
Size: 32 150 32 2641 32 1135
Fragments arranged by size:
2641 1135 150 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1819 1819
Size: 4022
XbaI T'CTAG_A
Cuts at: 1053 1053
Size: 4022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1517 1517
Size: 4022
XmaI C'CCGG_G
Cuts at: 1042 1042
Size: 4022
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI