Restriction map for
pF1KB6390
(Circular) (Six-base) MAPSORT of: pF1KB6390.seq Check: 4858 from: 1 to: 4016
pF1KB6390 4016 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1054 1054
Size: 4016
Acc65I G'GTAC_C
Cuts at: 1032 1032
Size: 4016
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1841 2273 3020 3288 901
Size: 940 432 747 268 1629
Fragments arranged by size:
1629 940 747 432 268
AflIII A'CryG_T
Cuts at: 1368 2472 3449 1368
Size: 1104 977 1935
Fragments arranged by size:
1935 1104 977
AgeI A'CCGG_T
Cuts at: 2404 2404
Size: 4016
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 297 329 297
Size: 32 3984
AlwNI CAG_nnn'CTG
Cuts at: 1321 2888 1321
Size: 1567 2449
ApaI G_GGCC'C
Cuts at: 147 207 394 147
Size: 60 187 3769
Fragments arranged by size:
3769 187 60
ApaLI G'TGCA_C
Cuts at: 2786 2786
Size: 4016
ApoI r'AATT_y
Cuts at: 1020 3369 3625 1020
Size: 2349 256 1411
Fragments arranged by size:
2349 1411 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4016
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 289 375 1036 289
Size: 86 661 3269
Fragments arranged by size:
3269 661 86
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 913 946 913
Size: 33 3983
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 154 358 1032 1446 1694 1729 154
Size: 204 674 414 248 35 2441
Fragments arranged by size:
2441 674 414 248 204 35
BanII G_rGCy'C
Cuts at: 147 168 207 394 1030 2060 147
Size: 21 39 187 636 1030 2103
Fragments arranged by size:
2103 1030 636 187 39 21
BbeI G_GCGC'C
Cuts at: 158 362 1450 1698 158
Size: 204 1088 248 2476
Fragments arranged by size:
2476 1088 248 204
BbsI GAAGACnn'nnnn_
Cuts at: 994 994
Size: 4016
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 179 213 90
Size: 34 55 34 3893
Fragments arranged by size:
3893 55 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1914 2675 4016 1914
Size: 761 1341 1914
Fragments arranged by size:
1914 1341 761
BclI T'GATC_A
Cuts at: 698 1536 698
Size: 838 3178
BglI GCCn_nnn'nGGC
Cuts at: 163 202 389 163
Size: 39 187 3790
Fragments arranged by size:
3790 187 39
BglII A'GATC_T
Cuts at: 669 1531 3568 669
Size: 862 2037 1117
Fragments arranged by size:
2037 1117 862
BlpI GC'TnA_GC
Cuts at: 1128 1128
Size: 4016
Bme1580I G_kGCm'C
Cuts at: 147 207 224 394 1641 1734 2790 147
Size: 60 17 170 1247 93 1056 1373
Fragments arranged by size:
1373 1247 1056 170 93 60 17
BmrI ACTGGGnnnn_n'
Cuts at: 466 585 1228 1309 1644 3788 466
Size: 119 643 81 335 2144 694
Fragments arranged by size:
2144 694 643 335 119 81
BmtI G_CTAG'C
Cuts at: 115 115
Size: 4016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 869 273
Size: 596 3420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2071 2563 2861 1192
Size: 879 492 298 2347
Fragments arranged by size:
2347 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3720 3720
Size: 4016
BsaAI yAC'GTr
Cuts at: 1369 1369
Size: 4016
BsaBI GATnn'nnATC
Cuts at: 6 1530 3567 6
Size: 1524 2037 455
Fragments arranged by size:
2037 1524 455
BsaHI Gr'CG_yC
Cuts at: 155 359 1447 1695 2397 3893 155
Size: 204 1088 248 702 1496 278
Fragments arranged by size:
1496 1088 702 278 248 204
BsaWI w'CCGG_w
Cuts at: 1205 1726 2404 2678 2825 3236 1205
Size: 521 678 274 147 411 1985
Fragments arranged by size:
1985 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 246 276 246
Size: 30 3986
BseYI C'CCAG_C
Cuts at: 200 406 1441 2199 2776 3272 200
Size: 206 1035 758 577 496 944
Fragments arranged by size:
1035 944 758 577 496 206
BsiEI CG_ry'CG
Cuts at: 104 181 269 350 1558 1604 2812 104
Size: 77 88 81 1208 46 1208 1308
Fragments arranged by size:
1308 1208 1208 88 81 77 46
BsiHKAI G_wGCw'C
Cuts at: 403 609 1030 1808 1998 2790 403
Size: 206 421 778 190 792 1629
Fragments arranged by size:
1629 792 778 421 206 190
Bsp1286I G_dGCh'C
Cuts at: 147 168 207 224 394 403 609 1030 1641
Size: 21 39 17 170 9 206 421 611
Cuts at: 1641 1734 1808 1998 2060 2790 147
Size: 93 74 190 62 730 1373
Fragments arranged by size:
1373 730 611 421 206 190 170 93
74 62 39 21 17 9
BspEI T'CCGG_A
Cuts at: 3236 3236
Size: 4016
BspHI T'CATG_A
Cuts at: 3192 3192
Size: 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1066 1280 1582 1963 1066
Size: 214 302 381 3119
Fragments arranged by size:
3119 381 302 214
BsrBI CCG'CTC
Cuts at: 50 271 355 2308 2362 3846 4011 50
Size: 221 84 1953 54 1484 165 55
Fragments arranged by size:
1953 1484 221 165 84 55 54
BsrDI GCAATG_nn'
Cuts at: 1928 1928
Size: 4016
BsrFI r'CCGG_y
Cuts at: 2014 2195 2404 2014
Size: 181 209 3626
Fragments arranged by size:
3626 209 181
BssHII G'CGCG_C
Cuts at: 127 127
Size: 4016
BssSI C'ACGA_G
Cuts at: 135 2287 2645 135
Size: 2152 358 1506
Fragments arranged by size:
2152 1506 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1393 1393
Size: 4016
BstBI TT'CG_AA
Cuts at: 2377 2377
Size: 4016
BstYI r'GATC_y
Cuts at: 1 669 1041 1508 1531 1866 2112 3124 3210
Size: 668 372 467 23 335 246 1012 86
Cuts at: 3210 3222 3568 1
Size: 12 346 449
Fragments arranged by size:
1012 668 467 449 372 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 424 424
Size: 4016
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1966 2147 2310 1966
Size: 181 163 3672
Fragments arranged by size:
3672 181 163
BtsI GCAGTG_nn'
Cuts at: 803 1259 803
Size: 456 3560
DraI TTT'AAA
Cuts at: 1015 1015
Size: 4016
DrdI GACnn_nn'nnGTC
Cuts at: 1722 2580 1722
Size: 858 3158
EaeI y'GGCC_r
Cuts at: 178 242 266 297 347 381 981 1601 1775
Size: 64 24 31 50 34 600 620 174
Cuts at: 1775 2166 2193 3538 178
Size: 391 27 1345 656
Fragments arranged by size:
1345 656 620 600 391 174 64 50
34 31 27 24
EagI C'GGCC_G
Cuts at: 178 266 347 1601 178
Size: 88 81 1254 2593
Fragments arranged by size:
2593 1254 88 81
EarI CTCTTCn'nnn_
Cuts at: 761 2039 2249 761
Size: 1278 210 2528
Fragments arranged by size:
2528 1278 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2224 2534 2680 3826 2224
Size: 310 146 1146 2414
Fragments arranged by size:
2414 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1028 1028
Size: 4016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 869 901 1841 2273 3020 3288 273
Size: 596 32 940 432 747 268 1001
Fragments arranged by size:
1001 940 747 596 432 268 32
EcoO109I rG'GnC_Cy
Cuts at: 143 144 204 390 1155 143
Size: 1 60 186 765 3004
Fragments arranged by size:
3004 765 186 60 1
EcoRI G'AATT_C
Cuts at: 1020 1020
Size: 4016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 611 2035 611
Size: 1424 2592
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 158 362 1450 1698 2720 3699 158
Size: 204 1088 248 1022 979 475
Fragments arranged by size:
1088 1022 979 475 248 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 246 278 1858 1888 1890 1920 246
Size: 32 1580 30 2 30 2342
Fragments arranged by size:
2342 1580 32 30 30 2
HincII GTy'rAC
Cuts at: 1055 1055
Size: 4016
KasI G'GCGC_C
Cuts at: 154 358 1446 1694 154
Size: 204 1088 248 2476
Fragments arranged by size:
2476 1088 248 204
KpnI G_GTAC'C
Cuts at: 1036 1036
Size: 4016
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 533 784 2687 2871 533
Size: 251 1903 184 1678
Fragments arranged by size:
1903 1678 251 184
MscI TGG'CCA
Cuts at: 1777 1777
Size: 4016
MslI CAynn'nnrTG
Cuts at: 2005 2132 2005
Size: 127 3889
MspA1I CmG'CkG
Cuts at: 303 346 364 952 1127 1441 1801 2814 3059
Size: 43 18 588 175 314 360 1013 245
Cuts at: 3059 303
Size: 1260
Fragments arranged by size:
1260 1013 588 360 314 245 175 43
18
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 155 359 1447 1695 155
Size: 204 1088 248 2476
Fragments arranged by size:
2476 1088 248 204
NcoI C'CATG_G
Cuts at: 424 424
Size: 4016
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NheI G'CTAG_C
Cuts at: 111 111
Size: 4016
NspI r_CATG'y
Cuts at: 1069 1569 2476 1069
Size: 500 907 2609
Fragments arranged by size:
2609 907 500
PciI A'CATG_T
Cuts at: 2472 2472
Size: 4016
PfoI T'CCnGG_A
Cuts at: 168 168
Size: 4016
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3984
PspOMI G'GGCC_C
Cuts at: 143 203 390 143
Size: 60 187 3769
Fragments arranged by size:
3769 187 60
PstI C_TGCA'G
Cuts at: 1063 1748 1063
Size: 685 3331
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 952 1441 1801 952
Size: 489 360 3167
Fragments arranged by size:
3167 489 360
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 1030 1030
Size: 4016
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 2039 2249 2039
Size: 210 3806
SbfI CC_TGCA'GG
Cuts at: 1063 1063
Size: 4016
ScaI AGT'ACT
Cuts at: 3533 3533
Size: 4016
SfcI C'TryA_G
Cuts at: 33 1059 1744 2737 2928 33
Size: 1026 685 993 191 1121
Fragments arranged by size:
1121 1026 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 389 389
Size: 4016
SfoI GGC'GCC
Cuts at: 156 360 1448 1696 156
Size: 204 1088 248 2476
Fragments arranged by size:
2476 1088 248 204
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 291 377 1038 291
Size: 86 661 3269
Fragments arranged by size:
3269 661 86
SmlI C'TyrA_G
Cuts at: 1171 2086 2578 2840 1171
Size: 915 492 262 2347
Fragments arranged by size:
2347 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 1069 1569 1069
Size: 500 3516
SspI AAT'ATT
Cuts at: 510 510
Size: 4016
StyI C'CwwG_G
Cuts at: 424 1150 1504 424
Size: 726 354 2936
Fragments arranged by size:
2936 726 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1599 1732 2191 2400 1599
Size: 133 459 209 3215
Fragments arranged by size:
3215 459 209 133
TatI w'GTAC_w
Cuts at: 3531 3531
Size: 4016
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 3984
Tth111I GACn'n_nGTC
Cuts at: 1813 1813
Size: 4016
XbaI T'CTAG_A
Cuts at: 1047 1047
Size: 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1511 1511
Size: 4016
XmaI C'CCGG_G
Cuts at: 289 375 1036 289
Size: 86 661 3269
Fragments arranged by size:
3269 661 86
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BbvCI BfrBI BmgBI Bpu10I BseRI BsgI BsiWI
BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI