Restriction map for
pF1KB6378
(Circular) (Six-base) MAPSORT of: pF1KB6378.seq Check: 8089 from: 1 to: 4010
pF1KB6378 4010 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 953 1048 953
Size: 95 3915
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1835 2267 3014 3282 1835
Size: 432 747 268 2563
Fragments arranged by size:
2563 747 432 268
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 739 739
Size: 4010
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 857 889 909 941 857
Size: 32 20 32 3926
Fragments arranged by size:
3926 32 32 20
AlwNI CAG_nnn'CTG
Cuts at: 1315 2882 1315
Size: 1567 2443
ApaI G_GGCC'C
Cuts at: 854 854
Size: 4010
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 1014 3363 3619 1014
Size: 2349 256 1405
Fragments arranged by size:
2349 1405 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 1030 1030
Size: 4010
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 1026 1440 1688 1723 1026
Size: 414 248 35 3313
Fragments arranged by size:
3313 414 248 35
BanII G_rGCy'C
Cuts at: 196 277 517 854 940 1024 2054 196
Size: 81 240 337 86 84 1030 2152
Fragments arranged by size:
2152 1030 337 240 86 84 81
BbeI G_GCGC'C
Cuts at: 1444 1692 1444
Size: 248 3762
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3976
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 161 495 1530 161
Size: 334 1035 2641
Fragments arranged by size:
2641 1035 334
BglI GCCn_nnn'nGGC
Cuts at: 560 736 560
Size: 176 3834
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 934 1122 934
Size: 188 3822
Bme1580I G_kGCm'C
Cuts at: 406 854 1635 1728 2784 406
Size: 448 781 93 1056 1632
Fragments arranged by size:
1632 1056 781 448 93
BmrI ACTGGGnnnn_n'
Cuts at: 1222 1303 1638 3782 1222
Size: 81 335 2144 1450
Fragments arranged by size:
2144 1450 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 825 2377 825
Size: 1552 2458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 545 197
Size: 348 3662
Bpu10I CC'TnA_GC
Cuts at: 195 195
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 605 3714 605
Size: 3109 901
BsaAI yAC'GTr
Cuts at: 1363 1363
Size: 4010
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 1441 1689 2391 3887 1441
Size: 248 702 1496 1564
Fragments arranged by size:
1564 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 238 259 268 289 238
Size: 21 9 21 3959
Fragments arranged by size:
3959 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 581 584 1012 581
Size: 3 428 3579
Fragments arranged by size:
3579 428 3
BseYI C'CCAG_C
Cuts at: 399 529 695 1435 2193 2770 3266 399
Size: 130 166 740 758 577 496 1143
Fragments arranged by size:
1143 758 740 577 496 166 130
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 196 940 1024 1802 1992 2784 196
Size: 744 84 778 190 792 1422
Fragments arranged by size:
1422 792 778 744 190 84
Bsp1286I G_dGCh'C
Cuts at: 196 277 406 517 854 940 1024 1635 1728
Size: 81 129 111 337 86 84 611 93
Cuts at: 1728 1802 1992 2054 2784 196
Size: 74 190 62 730 1422
Fragments arranged by size:
1422 730 611 337 190 129 111 93
86 84 81 74 62
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 731 1060 1274 1576 1957 731
Size: 329 214 302 381 2784
Fragments arranged by size:
2784 381 329 302 214
BsrBI CCG'CTC
Cuts at: 50 114 2302 2356 3840 4005 50
Size: 64 2188 54 1484 165 55
Fragments arranged by size:
2188 1484 165 64 55 54
BsrDI GCAATG_nn'
Cuts at: 788 1922 788
Size: 1134 2876
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstYI r'GATC_y
Cuts at: 1 1035 1502 1525 1860 2106 3118 3204 3216
Size: 1034 467 23 335 246 1012 86 12
Cuts at: 3216 3562 1
Size: 346 449
Fragments arranged by size:
1034 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 548 667 680 548
Size: 119 13 3878
Fragments arranged by size:
3878 119 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 474 1253 474
Size: 779 3231
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 1595 1769 2160 2187 3532 1595
Size: 174 391 27 1345 2073
Fragments arranged by size:
2073 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 136 2033 2243 136
Size: 1897 210 1903
Fragments arranged by size:
1903 1897 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 638 2218 2528 2674 3820 638
Size: 1580 310 146 1146 828
Fragments arranged by size:
1580 1146 828 310 146
EcoICRI GAG'CTC
Cuts at: 194 938 1022 194
Size: 744 84 3182
Fragments arranged by size:
3182 744 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 545 1835 2267 3014 3282 197
Size: 348 1290 432 747 268 925
Fragments arranged by size:
1290 925 747 432 348 268
EcoNI CCTnn'n_nnAGG
Cuts at: 771 771
Size: 4010
EcoO109I rG'GnC_Cy
Cuts at: 850 851 1149 850
Size: 1 298 3711
Fragments arranged by size:
3711 298 1
EcoRI G'AATT_C
Cuts at: 1014 1014
Size: 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 766 2029 766
Size: 1263 2747
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 1444 1692 2714 3693 1444
Size: 248 1022 979 1761
Fragments arranged by size:
1761 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 328 360 589 621 1852 1882 1884
Size: 32 58 32 229 32 1231 30 2
Cuts at: 1884 1914 238
Size: 30 2334
Fragments arranged by size:
2334 1231 229 58 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 690 1049 690
Size: 359 3651
KasI G'GCGC_C
Cuts at: 1440 1688 1440
Size: 248 3762
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 224 406 920 2681 2865 224
Size: 182 514 1761 184 1369
Fragments arranged by size:
1761 1369 514 184 182
MscI TGG'CCA
Cuts at: 1771 1771
Size: 4010
MslI CAynn'nnrTG
Cuts at: 335 1999 2126 335
Size: 1664 127 2219
Fragments arranged by size:
2219 1664 127
MspA1I CmG'CkG
Cuts at: 399 409 550 1121 1435 1795 2808 3053 399
Size: 10 141 571 314 360 1013 245 1356
Fragments arranged by size:
1356 1013 571 360 314 245 141 10
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 1441 1689 1441
Size: 248 3762
NcoI C'CATG_G
Cuts at: 667 680 667
Size: 13 3997
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 1063 1563 2470 1063
Size: 500 907 2603
Fragments arranged by size:
2603 907 500
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PspOMI G'GGCC_C
Cuts at: 850 850
Size: 4010
PstI C_TGCA'G
Cuts at: 1057 1742 1057
Size: 685 3325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 399 409 1435 1795 399
Size: 10 1026 360 2614
Fragments arranged by size:
2614 1026 360 10
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 196 940 1024 196
Size: 744 84 3182
Fragments arranged by size:
3182 744 84
SacII CC_GC'GG
Cuts at: 551 551
Size: 4010
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SfcI C'TryA_G
Cuts at: 33 950 1053 1738 2731 2922 33
Size: 917 103 685 993 191 1121
Fragments arranged by size:
1121 993 917 685 191 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 736 736
Size: 4010
SfoI GGC'GCC
Cuts at: 1442 1690 1442
Size: 248 3762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 1165 2080 2572 2834 1165
Size: 915 492 262 2341
Fragments arranged by size:
2341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StuI AGG'CCT
Cuts at: 808 903 808
Size: 95 3915
StyI C'CwwG_G
Cuts at: 229 667 680 980 1144 1498 229
Size: 438 13 300 164 354 2741
Fragments arranged by size:
2741 438 354 300 164 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1726 2185 2394 1593
Size: 133 459 209 3209
Fragments arranged by size:
3209 459 209 133
TatI w'GTAC_w
Cuts at: 3525 3525
Size: 4010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 514 843 1505 514
Size: 329 662 3019
Fragments arranged by size:
3019 662 329
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI