Restriction map for
pF1KB6376
(Circular) (Six-base) MAPSORT of: pF1KB6376.seq Check: 9033 from: 1 to: 4010
pF1KB6376 4010 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 703 1835 2267 3014 3282 520
Size: 183 1132 432 747 268 1248
Fragments arranged by size:
1248 1132 747 432 268 183
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 640 672 640
Size: 32 3978
AlwNI CAG_nnn'CTG
Cuts at: 439 996 1315 2882 439
Size: 557 319 1567 1567
Fragments arranged by size:
1567 1567 557 319
ApaI G_GGCC'C
Cuts at: 257 970 257
Size: 713 3297
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 478 1014 3363 3619 478
Size: 536 2349 256 869
Fragments arranged by size:
2349 869 536 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 1030 1030
Size: 4010
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 976 1026 1440 1688 1723 976
Size: 50 414 248 35 3263
Fragments arranged by size:
3263 414 248 50 35
BanII G_rGCy'C
Cuts at: 257 970 1024 2054 257
Size: 713 54 1030 2213
Fragments arranged by size:
2213 1030 713 54
BbeI G_GCGC'C
Cuts at: 1444 1692 1444
Size: 248 3762
BbvCI CC'TCA_GC
Cuts at: 119 392 119
Size: 273 3737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 706 740 706
Size: 34 3976
BciVI GTATCCnnnnn_n'
Cuts at: 713 1908 2669 4010 713
Size: 1195 761 1341 713
Fragments arranged by size:
1341 1195 761 713
BclI T'GATC_A
Cuts at: 998 1530 998
Size: 532 3478
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 257 970 1635 1728 2784 257
Size: 713 665 93 1056 1483
Fragments arranged by size:
1483 1056 713 665 93
BmrI ACTGGGnnnn_n'
Cuts at: 1222 1303 1638 3782 1222
Size: 81 335 2144 1450
Fragments arranged by size:
2144 1450 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 725 935 528
Size: 197 210 3603
Fragments arranged by size:
3603 210 197
Bpu10I CC'TnA_GC
Cuts at: 119 392 119
Size: 273 3737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 805 3714 805
Size: 2909 1101
BsaAI yAC'GTr
Cuts at: 1363 1363
Size: 4010
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 1441 1689 2391 3887 1441
Size: 248 702 1496 1564
Fragments arranged by size:
1564 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 640 670 640
Size: 30 3980
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 178
Size: 4010
BseYI C'CCAG_C
Cuts at: 213 1435 2193 2770 3266 213
Size: 1222 758 577 496 957
Fragments arranged by size:
1222 957 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 183 1024 1802 1992 2784 183
Size: 841 778 190 792 1409
Fragments arranged by size:
1409 841 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 183 257 970 1024 1635 1728 1802 1992 2054
Size: 74 713 54 611 93 74 190 62
Cuts at: 2054 2784 183
Size: 730 1409
Fragments arranged by size:
1409 730 713 611 190 93 74 74
62 54
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 1274 1576 1957 1060
Size: 214 302 381 3113
Fragments arranged by size:
3113 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 383 1922 383
Size: 1539 2471
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstXI CCAn_nnnn'nTGG
Cuts at: 576 576
Size: 4010
BstYI r'GATC_y
Cuts at: 1 864 1035 1502 1525 1860 2106 3118 3204
Size: 863 171 467 23 335 246 1012 86
Cuts at: 3204 3216 3562 1
Size: 12 346 449
Fragments arranged by size:
1012 863 467 449 346 335 246 171
86 23 12
BtgI C'CryG_G
Cuts at: 107 233 575 107
Size: 126 342 3542
Fragments arranged by size:
3542 342 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 278 356 462 1253 278
Size: 78 106 791 3035
Fragments arranged by size:
3035 791 106 78
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DraIII CAC_nnn'GTG
Cuts at: 411 508 976 411
Size: 97 468 3445
Fragments arranged by size:
3445 468 97
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 110 1595 1769 2160 2187 3532 110
Size: 1485 174 391 27 1345 588
Fragments arranged by size:
1485 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 256 301 554 2033 2243 256
Size: 45 253 1479 210 2023
Fragments arranged by size:
2023 1479 253 210 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1022 1022
Size: 4010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 528 703 725 935 1835 2267 3014 3282
Size: 8 175 22 210 900 432 747 268
Cuts at: 3282 520
Size: 1248
Fragments arranged by size:
1248 900 747 432 268 210 175 22
8
EcoO109I rG'GnC_Cy
Cuts at: 253 254 1149 253
Size: 1 895 3114
Fragments arranged by size:
3114 895 1
EcoRI G'AATT_C
Cuts at: 478 1014 478
Size: 536 3474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 1444 1692 2714 3693 1444
Size: 248 1022 979 1761
Fragments arranged by size:
1761 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 1852 1882 1884 1914 451
Size: 32 1369 30 2 30 2547
Fragments arranged by size:
2547 1369 32 30 30 2
HincII GTy'rAC
Cuts at: 1049 1049
Size: 4010
KasI G'GCGC_C
Cuts at: 1440 1688 1440
Size: 248 3762
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 533 2681 2865 533
Size: 2148 184 1678
Fragments arranged by size:
2148 1678 184
MscI TGG'CCA
Cuts at: 1771 1771
Size: 4010
MslI CAynn'nnrTG
Cuts at: 1999 2126 1999
Size: 127 3883
MspA1I CmG'CkG
Cuts at: 118 1121 1435 1795 2808 3053 118
Size: 1003 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 1003 360 314 245
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 1441 1689 1441
Size: 248 3762
NcoI C'CATG_G
Cuts at: 107 575 107
Size: 468 3542
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 552 1063 1563 2470 552
Size: 511 500 907 2092
Fragments arranged by size:
2092 907 511 500
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PflMI CCAn_nnn'nTGG
Cuts at: 411 411
Size: 4010
PfoI T'CCnGG_A
Cuts at: 320 320
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 640 672 3149 3181 640
Size: 32 2477 32 1469
Fragments arranged by size:
2477 1469 32 32
PspOMI G'GGCC_C
Cuts at: 253 966 253
Size: 713 3297
PstI C_TGCA'G
Cuts at: 444 1057 1742 444
Size: 613 685 2712
Fragments arranged by size:
2712 685 613
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 1435 1795 1435
Size: 360 3650
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 1024 1024
Size: 4010
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 554 2033 2243 554
Size: 1479 210 2321
Fragments arranged by size:
2321 1479 210
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SexAI A'CCwGG_T
Cuts at: 407 972 407
Size: 565 3445
SfcI C'TryA_G
Cuts at: 33 129 440 1053 1738 2731 2922 33
Size: 96 311 613 685 993 191 1121
Fragments arranged by size:
1121 993 685 613 311 191 96
SfoI GGC'GCC
Cuts at: 1442 1690 1442
Size: 248 3762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 1165 2080 2572 2834 1165
Size: 915 492 262 2341
Fragments arranged by size:
2341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StyI C'CwwG_G
Cuts at: 107 569 575 659 1144 1498 107
Size: 462 6 84 485 354 2619
Fragments arranged by size:
2619 485 462 354 84 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1726 2185 2394 1593
Size: 133 459 209 3209
Fragments arranged by size:
3209 459 209 133
TatI w'GTAC_w
Cuts at: 3525 3525
Size: 4010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1505 1505
Size: 4010
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
XmnI GAAnn'nnTTC
Cuts at: 316 316
Size: 4010
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI