Restriction map for
pF1KB6357
(Circular) (Six-base) MAPSORT of: pF1KB6357.seq Check: 9883 from: 1 to: 3914
pF1KB6357 3914 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 952 952
Size: 3914
Acc65I G'GTAC_C
Cuts at: 930 930
Size: 3914
AclI AA'CG_TT
Cuts at: 3754 3754
Size: 3914
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1739 2171 2918 3186 1739
Size: 432 747 268 2467
Fragments arranged by size:
2467 747 432 268
AflIII A'CryG_T
Cuts at: 509 1266 2370 3347 509
Size: 757 1104 977 1076
Fragments arranged by size:
1104 1076 977 757
AgeI A'CCGG_T
Cuts at: 2302 2302
Size: 3914
AlwNI CAG_nnn'CTG
Cuts at: 1219 2786 1219
Size: 1567 2347
ApaLI G'TGCA_C
Cuts at: 2684 2684
Size: 3914
ApoI r'AATT_y
Cuts at: 298 834 918 3267 3523 298
Size: 536 84 2349 256 689
Fragments arranged by size:
2349 689 536 256 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 3914
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
AvaI C'yCGr_G
Cuts at: 458 934 458
Size: 476 3438
BamHI G'GATC_C
Cuts at: 939 939
Size: 3914
BanI G'GyrC_C
Cuts at: 930 1344 1592 1627 930
Size: 414 248 35 3217
Fragments arranged by size:
3217 414 248 35
BanII G_rGCy'C
Cuts at: 594 928 1958 594
Size: 334 1030 2550
Fragments arranged by size:
2550 1030 334
BbeI G_GCGC'C
Cuts at: 1348 1596 1348
Size: 248 3666
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3880
BciVI GTATCCnnnnn_n'
Cuts at: 815 1812 2573 3914 815
Size: 997 761 1341 815
Fragments arranged by size:
1341 997 815 761
BclI T'GATC_A
Cuts at: 1434 1434
Size: 3914
BglII A'GATC_T
Cuts at: 1429 3466 1429
Size: 2037 1877
BlpI GC'TnA_GC
Cuts at: 1026 1026
Size: 3914
Bme1580I G_kGCm'C
Cuts at: 1539 1632 2688 1539
Size: 93 1056 2765
Fragments arranged by size:
2765 1056 93
BmgBI CAC'GTC
Cuts at: 339 339
Size: 3914
BmrI ACTGGGnnnn_n'
Cuts at: 1126 1207 1542 3686 1126
Size: 81 335 2144 1354
Fragments arranged by size:
2144 1354 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 3914
Bpu10I CC'TnA_GC
Cuts at: 212 212
Size: 3914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 268 1090 1969 2461 2759 208
Size: 60 822 879 492 298 1363
Fragments arranged by size:
1363 879 822 492 298 60
BsaI GGTCTCn'nnnn_
Cuts at: 667 3618 667
Size: 2951 963
BsaAI yAC'GTr
Cuts at: 1267 1267
Size: 3914
BsaBI GATnn'nnATC
Cuts at: 6 1428 3465 6
Size: 1422 2037 455
Fragments arranged by size:
2037 1422 455
BsaHI Gr'CG_yC
Cuts at: 1345 1593 2295 3791 1345
Size: 248 702 1496 1468
Fragments arranged by size:
1496 1468 702 248
BsaWI w'CCGG_w
Cuts at: 1103 1624 2302 2576 2723 3134 1103
Size: 521 678 274 147 411 1883
Fragments arranged by size:
1883 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 602 683 602
Size: 81 3833
BseYI C'CCAG_C
Cuts at: 1339 2097 2674 3170 1339
Size: 758 577 496 2083
Fragments arranged by size:
2083 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1456 1502 2710 104
Size: 1352 46 1208 1308
Fragments arranged by size:
1352 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 594 928 1706 1896 2688 594
Size: 334 778 190 792 1820
Fragments arranged by size:
1820 792 778 334 190
BsmI GAATG_Cn'
Cuts at: 866 866
Size: 3914
Bsp1286I G_dGCh'C
Cuts at: 594 928 1539 1632 1706 1896 1958 2688 594
Size: 334 611 93 74 190 62 730 1820
Fragments arranged by size:
1820 730 611 334 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3134 3134
Size: 3914
BspHI T'CATG_A
Cuts at: 569 747 3090 569
Size: 178 2343 1393
Fragments arranged by size:
2343 1393 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 964 1178 1480 1861 964
Size: 214 302 381 3017
Fragments arranged by size:
3017 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2206 2260 3744 3909 50
Size: 2156 54 1484 165 55
Fragments arranged by size:
2156 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1826 1826
Size: 3914
BsrFI r'CCGG_y
Cuts at: 1912 2093 2302 1912
Size: 181 209 3524
Fragments arranged by size:
3524 209 181
BssSI C'ACGA_G
Cuts at: 2185 2543 2185
Size: 358 3556
BstAPI GCAn_nnn'nTGC
Cuts at: 1291 1291
Size: 3914
BstBI TT'CG_AA
Cuts at: 2275 2275
Size: 3914
BstEII G'GTnAC_C
Cuts at: 432 432
Size: 3914
BstYI r'GATC_y
Cuts at: 1 524 825 939 1406 1429 1764 2010 3022
Size: 523 301 114 467 23 335 246 1012
Cuts at: 3022 3108 3120 3466 1
Size: 86 12 346 449
Fragments arranged by size:
1012 523 467 449 346 335 301 246
114 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 170 170
Size: 3914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1864 2045 2208 1864
Size: 181 163 3570
Fragments arranged by size:
3570 181 163
BtsI GCAGTG_nn'
Cuts at: 803 1157 803
Size: 354 3560
ClaI AT'CG_AT
Cuts at: 486 486
Size: 3914
DraI TTT'AAA
Cuts at: 506 840 913 506
Size: 334 73 3507
Fragments arranged by size:
3507 334 73
DrdI GACnn_nn'nnGTC
Cuts at: 1620 2478 1620
Size: 858 3056
EaeI y'GGCC_r
Cuts at: 756 1499 1673 2064 2091 3436 756
Size: 743 174 391 27 1345 1234
Fragments arranged by size:
1345 1234 743 391 174 27
EagI C'GGCC_G
Cuts at: 1499 1499
Size: 3914
EarI CTCTTCn'nnn_
Cuts at: 1937 2147 1937
Size: 210 3704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2122 2432 2578 3724 2122
Size: 310 146 1146 2312
Fragments arranged by size:
2312 1146 310 146
EcoICRI GAG'CTC
Cuts at: 592 926 592
Size: 334 3580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1739 2171 2918 3186 1739
Size: 432 747 268 2467
Fragments arranged by size:
2467 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 656 656
Size: 3914
EcoO109I rG'GnC_Cy
Cuts at: 1053 1053
Size: 3914
EcoRI G'AATT_C
Cuts at: 918 918
Size: 3914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1933 1933
Size: 3914
FspI TGC'GCA
Cuts at: 1695 1695
Size: 3914
HaeII r_GCGC'y
Cuts at: 1348 1596 2618 3597 1348
Size: 248 1022 979 1665
Fragments arranged by size:
1665 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 219 221 251 253 1756 1786 1788 1818 219
Size: 2 30 2 1503 30 2 30 2315
Fragments arranged by size:
2315 1503 30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 953 953
Size: 3914
KasI G'GCGC_C
Cuts at: 1344 1592 1344
Size: 248 3666
KpnI G_GTAC'C
Cuts at: 934 934
Size: 3914
MluI A'CGCG_T
Cuts at: 3347 3347
Size: 3914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 832 2585 2769 832
Size: 1753 184 1977
Fragments arranged by size:
1977 1753 184
MscI TGG'CCA
Cuts at: 758 1675 758
Size: 917 2997
MslI CAynn'nnrTG
Cuts at: 683 1903 2030 683
Size: 1220 127 2567
Fragments arranged by size:
2567 1220 127
MspA1I CmG'CkG
Cuts at: 165 1025 1339 1699 2712 2957 165
Size: 860 314 360 1013 245 1122
Fragments arranged by size:
1122 1013 860 360 314 245
NaeI GCC'GGC
Cuts at: 2095 2095
Size: 3914
NarI GG'CG_CC
Cuts at: 1345 1593 1345
Size: 248 3666
NgoMIV G'CCGG_C
Cuts at: 2093 2093
Size: 3914
NspI r_CATG'y
Cuts at: 513 967 1467 2374 513
Size: 454 500 907 2053
Fragments arranged by size:
2053 907 500 454
PciI A'CATG_T
Cuts at: 509 2370 509
Size: 1861 2053
PflMI CCAn_nnn'nTGG
Cuts at: 616 765 814 616
Size: 149 49 3716
Fragments arranged by size:
3716 149 49
PmeI GTTT'AAAC
Cuts at: 913 913
Size: 3914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3053 3085 3053
Size: 32 3882
PstI C_TGCA'G
Cuts at: 961 1646 961
Size: 685 3229
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3914
PvuII CAG'CTG
Cuts at: 1339 1699 1339
Size: 360 3554
RsrII CG'GwC_CG
Cuts at: 2109 2109
Size: 3914
SacI G_AGCT'C
Cuts at: 594 928 594
Size: 334 3580
SalI G'TCGA_C
Cuts at: 951 951
Size: 3914
SapI GCTCTTCn'nnn_
Cuts at: 1937 2147 1937
Size: 210 3704
SbfI CC_TGCA'GG
Cuts at: 961 961
Size: 3914
ScaI AGT'ACT
Cuts at: 3431 3431
Size: 3914
SfcI C'TryA_G
Cuts at: 33 196 438 957 1642 2635 2826 33
Size: 163 242 519 685 993 191 1121
Fragments arranged by size:
1121 993 685 519 242 191 163
SfoI GGC'GCC
Cuts at: 1346 1594 1346
Size: 248 3666
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
SmaI CCC'GGG
Cuts at: 936 936
Size: 3914
SmlI C'TyrA_G
Cuts at: 223 283 458 1069 1984 2476 2738 223
Size: 60 175 611 915 492 262 1399
Fragments arranged by size:
1399 915 611 492 262 175 60
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3914
SphI G_CATG'C
Cuts at: 967 1467 967
Size: 500 3414
StyI C'CwwG_G
Cuts at: 1048 1402 1048
Size: 354 3560
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1497 1630 2089 2298 1497
Size: 133 459 209 3113
Fragments arranged by size:
3113 459 209 133
TatI w'GTAC_w
Cuts at: 3429 3429
Size: 3914
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3431 3463 3431
Size: 32 3882
Tth111I GACn'n_nGTC
Cuts at: 1711 1711
Size: 3914
XbaI T'CTAG_A
Cuts at: 945 945
Size: 3914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1409 1409
Size: 3914
XhoI C'TCGA_G
Cuts at: 458 458
Size: 3914
XmaI C'CCGG_G
Cuts at: 934 934
Size: 3914
XmnI GAAnn'nnTTC
Cuts at: 894 894
Size: 3914
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmtI BpmI BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I BtgI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI