Restriction map for
pF1KB6355
(Circular) (Six-base) MAPSORT of: pF1KB6355.seq Check: 8889 from: 1 to: 3911
pF1KB6355 3911 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 949 949
Size: 3911
Acc65I G'GTAC_C
Cuts at: 927 927
Size: 3911
AclI AA'CG_TT
Cuts at: 3751 3751
Size: 3911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1736 2168 2915 3183 1736
Size: 432 747 268 2464
Fragments arranged by size:
2464 747 432 268
AflIII A'CryG_T
Cuts at: 903 1263 2367 3344 903
Size: 360 1104 977 1470
Fragments arranged by size:
1470 1104 977 360
AgeI A'CCGG_T
Cuts at: 2299 2299
Size: 3911
AlwNI CAG_nnn'CTG
Cuts at: 1216 2783 1216
Size: 1567 2344
ApaLI G'TGCA_C
Cuts at: 2681 2681
Size: 3911
ApoI r'AATT_y
Cuts at: 227 273 350 423 525 583 714 915 3264
Size: 46 77 73 102 58 131 201 2349
Cuts at: 3264 3520 227
Size: 256 618
Fragments arranged by size:
2349 618 256 201 131 102 77 73
58 46
AseI AT'TA_AT
Cuts at: 20 20
Size: 3911
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
AvaI C'yCGr_G
Cuts at: 931 931
Size: 3911
BamHI G'GATC_C
Cuts at: 936 936
Size: 3911
BanI G'GyrC_C
Cuts at: 674 927 1341 1589 1624 674
Size: 253 414 248 35 2961
Fragments arranged by size:
2961 414 253 248 35
BanII G_rGCy'C
Cuts at: 182 925 1955 182
Size: 743 1030 2138
Fragments arranged by size:
2138 1030 743
BbeI G_GCGC'C
Cuts at: 678 1345 1593 678
Size: 667 248 2996
Fragments arranged by size:
2996 667 248
BbsI GAAGACnn'nnnn_
Cuts at: 650 827 650
Size: 177 3734
BciVI GTATCCnnnnn_n'
Cuts at: 1809 2570 3911 1809
Size: 761 1341 1809
Fragments arranged by size:
1809 1341 761
BclI T'GATC_A
Cuts at: 768 1431 768
Size: 663 3248
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3911
BglII A'GATC_T
Cuts at: 505 1426 3463 505
Size: 921 2037 953
Fragments arranged by size:
2037 953 921
BlpI GC'TnA_GC
Cuts at: 1023 1023
Size: 3911
Bme1580I G_kGCm'C
Cuts at: 1536 1629 2685 1536
Size: 93 1056 2762
Fragments arranged by size:
2762 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 663 1123 1204 1539 3683 663
Size: 460 81 335 2144 891
Fragments arranged by size:
2144 891 460 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 3911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 861 503
Size: 358 3553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1966 2458 2756 1087
Size: 879 492 298 2242
Fragments arranged by size:
2242 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3615 3615
Size: 3911
BsaAI yAC'GTr
Cuts at: 1264 1264
Size: 3911
BsaBI GATnn'nnATC
Cuts at: 6 1425 3462 6
Size: 1419 2037 455
Fragments arranged by size:
2037 1419 455
BsaHI Gr'CG_yC
Cuts at: 675 1342 1590 2292 3788 675
Size: 667 248 702 1496 798
Fragments arranged by size:
1496 798 702 667 248
BsaWI w'CCGG_w
Cuts at: 1100 1621 2299 2573 2720 3131 1100
Size: 521 678 274 147 411 1880
Fragments arranged by size:
1880 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 190
Size: 3911
BseYI C'CCAG_C
Cuts at: 205 1336 2094 2671 3167 205
Size: 1131 758 577 496 949
Fragments arranged by size:
1131 949 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1453 1499 2707 104
Size: 1349 46 1208 1308
Fragments arranged by size:
1349 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 182 925 1703 1893 2685 182
Size: 743 778 190 792 1408
Fragments arranged by size:
1408 792 778 743 190
Bsp1286I G_dGCh'C
Cuts at: 182 925 1536 1629 1703 1893 1955 2685 182
Size: 743 611 93 74 190 62 730 1408
Fragments arranged by size:
1408 743 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3131 3131
Size: 3911
BspHI T'CATG_A
Cuts at: 831 3087 831
Size: 2256 1655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 443 961 1175 1477 1858 443
Size: 518 214 302 381 2496
Fragments arranged by size:
2496 518 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2203 2257 3741 3906 50
Size: 2153 54 1484 165 55
Fragments arranged by size:
2153 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 311 1823 311
Size: 1512 2399
BsrFI r'CCGG_y
Cuts at: 1909 2090 2299 1909
Size: 181 209 3521
Fragments arranged by size:
3521 209 181
BssSI C'ACGA_G
Cuts at: 2182 2540 2182
Size: 358 3553
BstAPI GCAn_nnn'nTGC
Cuts at: 1288 1288
Size: 3911
BstBI TT'CG_AA
Cuts at: 2272 2272
Size: 3911
BstXI CCAn_nnnn'nTGG
Cuts at: 205 205
Size: 3911
BstYI r'GATC_y
Cuts at: 1 505 936 1403 1426 1761 2007 3019 3105
Size: 504 431 467 23 335 246 1012 86
Cuts at: 3105 3117 3463 1
Size: 12 346 449
Fragments arranged by size:
1012 504 467 449 431 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3911
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1861 2042 2205 1861
Size: 181 163 3567
Fragments arranged by size:
3567 181 163
BtsI GCAGTG_nn'
Cuts at: 548 1154 548
Size: 606 3305
DraI TTT'AAA
Cuts at: 910 910
Size: 3911
DrdI GACnn_nn'nnGTC
Cuts at: 1617 2475 1617
Size: 858 3053
EaeI y'GGCC_r
Cuts at: 1496 1670 2061 2088 3433 1496
Size: 174 391 27 1345 1974
Fragments arranged by size:
1974 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1496 1496
Size: 3911
EarI CTCTTCn'nnn_
Cuts at: 519 858 1934 2144 519
Size: 339 1076 210 2286
Fragments arranged by size:
2286 1076 339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 139 2119 2429 2575 3721 139
Size: 1980 310 146 1146 329
Fragments arranged by size:
1980 1146 329 310 146
EcoICRI GAG'CTC
Cuts at: 180 923 180
Size: 743 3168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 861 1736 2168 2915 3183 503
Size: 358 875 432 747 268 1231
Fragments arranged by size:
1231 875 747 432 358 268
EcoO109I rG'GnC_Cy
Cuts at: 1050 1050
Size: 3911
EcoRI G'AATT_C
Cuts at: 714 915 714
Size: 201 3710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1930 1930
Size: 3911
FspI TGC'GCA
Cuts at: 1692 1692
Size: 3911
HaeII r_GCGC'y
Cuts at: 678 894 1345 1593 2615 3594 678
Size: 216 451 248 1022 979 995
Fragments arranged by size:
1022 995 979 451 248 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 760 792 1753 1783 1785 1815 760
Size: 32 961 30 2 30 2856
Fragments arranged by size:
2856 961 32 30 30 2
HincII GTy'rAC
Cuts at: 950 950
Size: 3911
KasI G'GCGC_C
Cuts at: 674 1341 1589 674
Size: 667 248 2996
Fragments arranged by size:
2996 667 248
KpnI G_GTAC'C
Cuts at: 931 931
Size: 3911
MluI A'CGCG_T
Cuts at: 3344 3344
Size: 3911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 399 2582 2766 399
Size: 2183 184 1544
Fragments arranged by size:
2183 1544 184
MscI TGG'CCA
Cuts at: 1672 1672
Size: 3911
MslI CAynn'nnrTG
Cuts at: 851 1900 2027 851
Size: 1049 127 2735
Fragments arranged by size:
2735 1049 127
MspA1I CmG'CkG
Cuts at: 1022 1336 1696 2709 2954 1022
Size: 314 360 1013 245 1979
Fragments arranged by size:
1979 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2092 2092
Size: 3911
NarI GG'CG_CC
Cuts at: 675 1342 1590 675
Size: 667 248 2996
Fragments arranged by size:
2996 667 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3911
NdeI CA'TA_TG
Cuts at: 573 573
Size: 3911
NgoMIV G'CCGG_C
Cuts at: 2090 2090
Size: 3911
NspI r_CATG'y
Cuts at: 964 1464 2371 964
Size: 500 907 2504
Fragments arranged by size:
2504 907 500
PciI A'CATG_T
Cuts at: 2367 2367
Size: 3911
PmeI GTTT'AAAC
Cuts at: 910 910
Size: 3911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3879
PshAI GACnn'nnGTC
Cuts at: 567 567
Size: 3911
PstI C_TGCA'G
Cuts at: 958 1643 958
Size: 685 3226
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3911
PvuII CAG'CTG
Cuts at: 1336 1696 1336
Size: 360 3551
RsrII CG'GwC_CG
Cuts at: 2106 2106
Size: 3911
SacI G_AGCT'C
Cuts at: 182 925 182
Size: 743 3168
SalI G'TCGA_C
Cuts at: 948 948
Size: 3911
SapI GCTCTTCn'nnn_
Cuts at: 1934 2144 1934
Size: 210 3701
SbfI CC_TGCA'GG
Cuts at: 958 958
Size: 3911
ScaI AGT'ACT
Cuts at: 3428 3428
Size: 3911
SfcI C'TryA_G
Cuts at: 33 954 1639 2632 2823 33
Size: 921 685 993 191 1121
Fragments arranged by size:
1121 993 921 685 191
SfoI GGC'GCC
Cuts at: 676 1343 1591 676
Size: 667 248 2996
Fragments arranged by size:
2996 667 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
SmaI CCC'GGG
Cuts at: 933 933
Size: 3911
SmlI C'TyrA_G
Cuts at: 1066 1981 2473 2735 1066
Size: 915 492 262 2242
Fragments arranged by size:
2242 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3911
SphI G_CATG'C
Cuts at: 964 1464 964
Size: 500 3411
StuI AGG'CCT
Cuts at: 342 342
Size: 3911
StyI C'CwwG_G
Cuts at: 107 678 1045 1399 107
Size: 571 367 354 2619
Fragments arranged by size:
2619 571 367 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 187 1494 1627 2086 2295 187
Size: 1307 133 459 209 1803
Fragments arranged by size:
1803 1307 459 209 133
TatI w'GTAC_w
Cuts at: 3426 3426
Size: 3911
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 3879
Tth111I GACn'n_nGTC
Cuts at: 1708 1708
Size: 3911
XbaI T'CTAG_A
Cuts at: 942 942
Size: 3911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 415 1406 415
Size: 991 2920
XmaI C'CCGG_G
Cuts at: 931 931
Size: 3911
XmnI GAAnn'nnTTC
Cuts at: 527 714 527
Size: 187 3724
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI