Restriction map for
pF1KB6351
(Circular) (Six-base) MAPSORT of: pF1KB6351.seq Check: 995 from: 1 to: 3911
pF1KB6351 3911 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 949 949
Size: 3911
Acc65I G'GTAC_C
Cuts at: 927 927
Size: 3911
AclI AA'CG_TT
Cuts at: 3751 3751
Size: 3911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1736 2168 2915 3183 1736
Size: 432 747 268 2464
Fragments arranged by size:
2464 747 432 268
AflIII A'CryG_T
Cuts at: 903 1263 2367 3344 903
Size: 360 1104 977 1470
Fragments arranged by size:
1470 1104 977 360
AgeI A'CCGG_T
Cuts at: 2299 2299
Size: 3911
AlwNI CAG_nnn'CTG
Cuts at: 280 1216 2783 280
Size: 936 1567 1408
Fragments arranged by size:
1567 1408 936
ApaLI G'TGCA_C
Cuts at: 2681 2681
Size: 3911
ApoI r'AATT_y
Cuts at: 915 3264 3520 915
Size: 2349 256 1306
Fragments arranged by size:
2349 1306 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3911
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
AvaI C'yCGr_G
Cuts at: 137 312 493 931 137
Size: 175 181 438 3117
Fragments arranged by size:
3117 438 181 175
BamHI G'GATC_C
Cuts at: 936 936
Size: 3911
BanI G'GyrC_C
Cuts at: 269 927 1341 1589 1624 269
Size: 658 414 248 35 2556
Fragments arranged by size:
2556 658 414 248 35
BanII G_rGCy'C
Cuts at: 925 1955 925
Size: 1030 2881
BbeI G_GCGC'C
Cuts at: 1345 1593 1345
Size: 248 3663
BbsI GAAGACnn'nnnn_
Cuts at: 518 623 518
Size: 105 3806
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 449 483 449
Size: 34 3877
BciVI GTATCCnnnnn_n'
Cuts at: 839 1809 2570 3911 839
Size: 970 761 1341 839
Fragments arranged by size:
1341 970 839 761
BclI T'GATC_A
Cuts at: 531 588 1431 531
Size: 57 843 3011
Fragments arranged by size:
3011 843 57
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3911
BglII A'GATC_T
Cuts at: 731 1426 3463 731
Size: 695 2037 1179
Fragments arranged by size:
2037 1179 695
BlpI GC'TnA_GC
Cuts at: 1023 1023
Size: 3911
Bme1580I G_kGCm'C
Cuts at: 272 1536 1629 2685 272
Size: 1264 93 1056 1498
Fragments arranged by size:
1498 1264 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1123 1204 1539 3683 1123
Size: 81 335 2144 1351
Fragments arranged by size:
2144 1351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 3911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1966 2458 2756 1087
Size: 879 492 298 2242
Fragments arranged by size:
2242 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3615 3615
Size: 3911
BsaAI yAC'GTr
Cuts at: 1264 1264
Size: 3911
BsaBI GATnn'nnATC
Cuts at: 6 536 860 1425 3462 6
Size: 530 324 565 2037 455
Fragments arranged by size:
2037 565 530 455 324
BsaHI Gr'CG_yC
Cuts at: 1342 1590 2292 3788 1342
Size: 248 702 1496 1465
Fragments arranged by size:
1496 1465 702 248
BsaWI w'CCGG_w
Cuts at: 677 1100 1621 2299 2573 2720 3131 677
Size: 423 521 678 274 147 411 1457
Fragments arranged by size:
1457 678 521 423 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 563 388
Size: 175 3736
BseYI C'CCAG_C
Cuts at: 186 252 1336 2094 2671 3167 186
Size: 66 1084 758 577 496 930
Fragments arranged by size:
1084 930 758 577 496 66
BsiEI CG_ry'CG
Cuts at: 104 1453 1499 2707 104
Size: 1349 46 1208 1308
Fragments arranged by size:
1349 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 925 1703 1893 2685 925
Size: 778 190 792 2151
Fragments arranged by size:
2151 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 272 925 1536 1629 1703 1893 1955 2685 272
Size: 653 611 93 74 190 62 730 1498
Fragments arranged by size:
1498 730 653 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3131 3131
Size: 3911
BspHI T'CATG_A
Cuts at: 3087 3087
Size: 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 248 961 1175 1477 1858 248
Size: 713 214 302 381 2301
Fragments arranged by size:
2301 713 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2203 2257 3741 3906 50
Size: 2153 54 1484 165 55
Fragments arranged by size:
2153 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1823 1823
Size: 3911
BsrFI r'CCGG_y
Cuts at: 1909 2090 2299 1909
Size: 181 209 3521
Fragments arranged by size:
3521 209 181
BssSI C'ACGA_G
Cuts at: 2182 2540 2182
Size: 358 3553
BstAPI GCAn_nnn'nTGC
Cuts at: 1288 1288
Size: 3911
BstBI TT'CG_AA
Cuts at: 2272 2272
Size: 3911
BstYI r'GATC_y
Cuts at: 1 731 936 1403 1426 1761 2007 3019 3105
Size: 730 205 467 23 335 246 1012 86
Cuts at: 3105 3117 3463 1
Size: 12 346 449
Fragments arranged by size:
1012 730 467 449 346 335 246 205
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 696 696
Size: 3911
BtgI C'CryG_G
Cuts at: 411 411
Size: 3911
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 166 1861 2042 2205 166
Size: 1695 181 163 1872
Fragments arranged by size:
1872 1695 181 163
BtsI GCAGTG_nn'
Cuts at: 1154 1154
Size: 3911
DraI TTT'AAA
Cuts at: 910 910
Size: 3911
DrdI GACnn_nn'nnGTC
Cuts at: 1617 2475 1617
Size: 858 3053
EaeI y'GGCC_r
Cuts at: 174 1496 1670 2061 2088 3433 174
Size: 1322 174 391 27 1345 652
Fragments arranged by size:
1345 1322 652 391 174 27
EagI C'GGCC_G
Cuts at: 1496 1496
Size: 3911
EarI CTCTTCn'nnn_
Cuts at: 1934 2144 1934
Size: 210 3701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2119 2429 2575 3721 2119
Size: 310 146 1146 2309
Fragments arranged by size:
2309 1146 310 146
EcoICRI GAG'CTC
Cuts at: 923 923
Size: 3911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1736 2168 2915 3183 1736
Size: 432 747 268 2464
Fragments arranged by size:
2464 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1050 1050
Size: 3911
EcoRI G'AATT_C
Cuts at: 915 915
Size: 3911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1930 1930
Size: 3911
FspI TGC'GCA
Cuts at: 363 1692 363
Size: 1329 2582
HaeII r_GCGC'y
Cuts at: 200 263 293 459 1345 1593 2615 3594 200
Size: 63 30 166 886 248 1022 979 517
Fragments arranged by size:
1022 979 886 517 248 166 63 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 679 711 1753 1783 1785 1815 679
Size: 32 1042 30 2 30 2775
Fragments arranged by size:
2775 1042 32 30 30 2
HincII GTy'rAC
Cuts at: 950 950
Size: 3911
HindIII A'AGCT_T
Cuts at: 576 576
Size: 3911
KasI G'GCGC_C
Cuts at: 1341 1589 1341
Size: 248 3663
KpnI G_GTAC'C
Cuts at: 931 931
Size: 3911
MluI A'CGCG_T
Cuts at: 3344 3344
Size: 3911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 2582 2766 248
Size: 2334 184 1393
Fragments arranged by size:
2334 1393 184
MscI TGG'CCA
Cuts at: 1672 1672
Size: 3911
MslI CAynn'nnrTG
Cuts at: 1900 2027 1900
Size: 127 3784
MspA1I CmG'CkG
Cuts at: 127 252 413 1022 1336 1696 2709 2954 127
Size: 125 161 609 314 360 1013 245 1084
Fragments arranged by size:
1084 1013 609 360 314 245 161 125
NaeI GCC'GGC
Cuts at: 2092 2092
Size: 3911
NarI GG'CG_CC
Cuts at: 1342 1590 1342
Size: 248 3663
NgoMIV G'CCGG_C
Cuts at: 2090 2090
Size: 3911
NruI TCG'CGA
Cuts at: 151 151
Size: 3911
NspI r_CATG'y
Cuts at: 474 964 1464 2371 474
Size: 490 500 907 2014
Fragments arranged by size:
2014 907 500 490
PciI A'CATG_T
Cuts at: 2367 2367
Size: 3911
PmeI GTTT'AAAC
Cuts at: 910 910
Size: 3911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3879
PstI C_TGCA'G
Cuts at: 285 451 958 1643 285
Size: 166 507 685 2553
Fragments arranged by size:
2553 685 507 166
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3911
PvuII CAG'CTG
Cuts at: 1336 1696 1336
Size: 360 3551
RsrII CG'GwC_CG
Cuts at: 2106 2106
Size: 3911
SacI G_AGCT'C
Cuts at: 925 925
Size: 3911
SacII CC_GC'GG
Cuts at: 414 414
Size: 3911
SalI G'TCGA_C
Cuts at: 948 948
Size: 3911
SapI GCTCTTCn'nnn_
Cuts at: 1934 2144 1934
Size: 210 3701
SbfI CC_TGCA'GG
Cuts at: 451 958 451
Size: 507 3404
ScaI AGT'ACT
Cuts at: 394 3428 394
Size: 3034 877
SfcI C'TryA_G
Cuts at: 33 281 447 954 1639 2632 2823 33
Size: 248 166 507 685 993 191 1121
Fragments arranged by size:
1121 993 685 507 248 191 166
SfoI GGC'GCC
Cuts at: 1343 1591 1343
Size: 248 3663
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
SmaI CCC'GGG
Cuts at: 139 314 495 933 139
Size: 175 181 438 3117
Fragments arranged by size:
3117 438 181 175
SmlI C'TyrA_G
Cuts at: 1066 1981 2473 2735 1066
Size: 915 492 262 2242
Fragments arranged by size:
2242 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3911
SphI G_CATG'C
Cuts at: 474 964 1464 474
Size: 490 500 2921
Fragments arranged by size:
2921 500 490
SspI AAT'ATT
Cuts at: 806 806
Size: 3911
StyI C'CwwG_G
Cuts at: 599 1045 1399 599
Size: 446 354 3111
Fragments arranged by size:
3111 446 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 287 1494 1627 2086 2295 287
Size: 1207 133 459 209 1903
Fragments arranged by size:
1903 1207 459 209 133
TatI w'GTAC_w
Cuts at: 392 3426 392
Size: 3034 877
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 361 393 506 538 709 741 3428 3460 361
Size: 32 113 32 171 32 2687 32 812
Fragments arranged by size:
2687 812 171 113 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1708 1708
Size: 3911
XbaI T'CTAG_A
Cuts at: 942 942
Size: 3911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1406 114
Size: 1292 2619
XmaI C'CCGG_G
Cuts at: 137 312 493 931 137
Size: 175 181 438 3117
Fragments arranged by size:
3117 438 181 175
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NruI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI