Restriction map for
pF1KB6346
(Circular) (Six-base) MAPSORT of: pF1KB6346.seq Check: 2508 from: 1 to: 3986
pF1KB6346 3986 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 911 911
Size: 3986
AccI GT'mk_AC
Cuts at: 1024 1024
Size: 3986
Acc65I G'GTAC_C
Cuts at: 1002 1002
Size: 3986
AclI AA'CG_TT
Cuts at: 3826 3826
Size: 3986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 1811 2243 2990 3258 115
Size: 1696 432 747 268 843
Fragments arranged by size:
1696 843 747 432 268
AflIII A'CryG_T
Cuts at: 1338 2442 3419 1338
Size: 1104 977 1905
Fragments arranged by size:
1905 1104 977
AgeI A'CCGG_T
Cuts at: 2374 2374
Size: 3986
AlwNI CAG_nnn'CTG
Cuts at: 138 1291 2858 138
Size: 1153 1567 1266
Fragments arranged by size:
1567 1266 1153
ApaI G_GGCC'C
Cuts at: 767 767
Size: 3986
ApaLI G'TGCA_C
Cuts at: 2756 2756
Size: 3986
ApoI r'AATT_y
Cuts at: 703 990 3339 3595 703
Size: 287 2349 256 1094
Fragments arranged by size:
2349 1094 287 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
AvaI C'yCGr_G
Cuts at: 1006 1006
Size: 3986
BamHI G'GATC_C
Cuts at: 286 1011 286
Size: 725 3261
BanI G'GyrC_C
Cuts at: 1002 1416 1664 1699 1002
Size: 414 248 35 3289
Fragments arranged by size:
3289 414 248 35
BanII G_rGCy'C
Cuts at: 767 854 1000 2030 767
Size: 87 146 1030 2723
Fragments arranged by size:
2723 1030 146 87
BbeI G_GCGC'C
Cuts at: 1420 1668 1420
Size: 248 3738
BbvCI CC'TCA_GC
Cuts at: 250 250
Size: 3986
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 853 887 853
Size: 34 3952
BciVI GTATCCnnnnn_n'
Cuts at: 1884 2645 3986 1884
Size: 761 1341 1884
Fragments arranged by size:
1884 1341 761
BclI T'GATC_A
Cuts at: 1506 1506
Size: 3986
BglII A'GATC_T
Cuts at: 1501 3538 1501
Size: 2037 1949
BlpI GC'TnA_GC
Cuts at: 1098 1098
Size: 3986
Bme1580I G_kGCm'C
Cuts at: 767 876 1611 1704 2760 767
Size: 109 735 93 1056 1993
Fragments arranged by size:
1993 1056 735 109 93
BmgBI CAC'GTC
Cuts at: 686 686
Size: 3986
BmrI ACTGGGnnnn_n'
Cuts at: 588 1198 1279 1614 3758 588
Size: 610 81 335 2144 816
Fragments arranged by size:
2144 816 610 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 3986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 454 523 679 834 453
Size: 1 69 156 155 3605
Fragments arranged by size:
3605 156 155 69 1
Bpu10I CC'TnA_GC
Cuts at: 250 517 250
Size: 267 3719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 1162 2041 2533 2831 399
Size: 763 879 492 298 1554
Fragments arranged by size:
1554 879 763 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3690 3690
Size: 3986
BsaAI yAC'GTr
Cuts at: 486 1339 486
Size: 853 3133
BsaBI GATnn'nnATC
Cuts at: 6 1500 3537 6
Size: 1494 2037 455
Fragments arranged by size:
2037 1494 455
BsaHI Gr'CG_yC
Cuts at: 638 908 1417 1665 2367 3863 638
Size: 270 509 248 702 1496 761
Fragments arranged by size:
1496 761 702 509 270 248
BsaWI w'CCGG_w
Cuts at: 217 1175 1696 2374 2648 2795 3206 217
Size: 958 521 678 274 147 411 997
Fragments arranged by size:
997 958 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 212 242 383 413 212
Size: 30 141 30 3785
Fragments arranged by size:
3785 141 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 176 220 325 709 872 163
Size: 13 44 105 384 163 3277
Fragments arranged by size:
3277 384 163 105 44 13
BseYI C'CCAG_C
Cuts at: 191 299 428 929 1411 2169 2746 3242 191
Size: 108 129 501 482 758 577 496 935
Fragments arranged by size:
935 758 577 501 496 482 129 108
BsiEI CG_ry'CG
Cuts at: 104 894 1528 1574 2782 104
Size: 790 634 46 1208 1308
Fragments arranged by size:
1308 1208 790 634 46
BsiHKAI G_wGCw'C
Cuts at: 358 385 590 1000 1778 1968 2760 358
Size: 27 205 410 778 190 792 1584
Fragments arranged by size:
1584 792 778 410 205 190 27
BsmBI CGTCTCn'nnnn_
Cuts at: 214 325 790 214
Size: 111 465 3410
Fragments arranged by size:
3410 465 111
Bsp1286I G_dGCh'C
Cuts at: 358 385 590 767 854 876 1000 1611 1704
Size: 27 205 177 87 22 124 611 93
Cuts at: 1704 1778 1968 2030 2760 358
Size: 74 190 62 730 1584
Fragments arranged by size:
1584 730 611 205 190 177 124 93
87 74 62 27 22
BspEI T'CCGG_A
Cuts at: 217 3206 217
Size: 2989 997
BspHI T'CATG_A
Cuts at: 3162 3162
Size: 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 515 571 1036 1250 1552 1933 515
Size: 56 465 214 302 381 2568
Fragments arranged by size:
2568 465 381 302 214 56
BsrBI CCG'CTC
Cuts at: 50 2278 2332 3816 3981 50
Size: 2228 54 1484 165 55
Fragments arranged by size:
2228 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 402 552 1898 402
Size: 150 1346 2490
Fragments arranged by size:
2490 1346 150
BsrFI r'CCGG_y
Cuts at: 653 1984 2165 2374 653
Size: 1331 181 209 2265
Fragments arranged by size:
2265 1331 209 181
BssSI C'ACGA_G
Cuts at: 589 2257 2615 589
Size: 1668 358 1960
Fragments arranged by size:
1960 1668 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1363 1363
Size: 3986
BstBI TT'CG_AA
Cuts at: 2347 2347
Size: 3986
BstXI CCAn_nnnn'nTGG
Cuts at: 547 547
Size: 3986
BstYI r'GATC_y
Cuts at: 1 286 1011 1478 1501 1836 2082 3094 3180
Size: 285 725 467 23 335 246 1012 86
Cuts at: 3180 3192 3538 1
Size: 12 346 449
Fragments arranged by size:
1012 725 467 449 346 335 285 246
86 23 12
BtgI C'CryG_G
Cuts at: 846 846
Size: 3986
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1936 2117 2280 1936
Size: 181 163 3642
Fragments arranged by size:
3642 181 163
BtsI GCAGTG_nn'
Cuts at: 1229 1229
Size: 3986
DraI TTT'AAA
Cuts at: 985 985
Size: 3986
DraIII CAC_nnn'GTG
Cuts at: 675 675
Size: 3986
DrdI GACnn_nn'nnGTC
Cuts at: 1692 2550 1692
Size: 858 3128
EaeI y'GGCC_r
Cuts at: 843 891 1571 1745 2136 2163 3508 843
Size: 48 680 174 391 27 1345 1321
Fragments arranged by size:
1345 1321 680 391 174 48 27
EagI C'GGCC_G
Cuts at: 891 1571 891
Size: 680 3306
EarI CTCTTCn'nnn_
Cuts at: 2009 2219 2009
Size: 210 3776
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2194 2504 2650 3796 2194
Size: 310 146 1146 2384
Fragments arranged by size:
2384 1146 310 146
EcoICRI GAG'CTC
Cuts at: 998 998
Size: 3986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 453 454 523 679 834 1811 2243 2990
Size: 338 1 69 156 155 977 432 747
Cuts at: 2990 3258 115
Size: 268 843
Fragments arranged by size:
977 843 747 432 338 268 156 155
69 1
EcoO109I rG'GnC_Cy
Cuts at: 321 450 560 724 764 920 1125 321
Size: 129 110 164 40 156 205 3182
Fragments arranged by size:
3182 205 164 156 129 110 40
EcoRI G'AATT_C
Cuts at: 990 990
Size: 3986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3986
FspI TGC'GCA
Cuts at: 1767 1767
Size: 3986
HaeII r_GCGC'y
Cuts at: 538 1420 1668 2690 3669 538
Size: 882 248 1022 979 855
Fragments arranged by size:
1022 979 882 855 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 212 244 447 479 1828 1858 1860 1890 212
Size: 32 203 32 1349 30 2 30 2308
Fragments arranged by size:
2308 1349 203 32 32 30 30 2
HincII GTy'rAC
Cuts at: 576 1025 576
Size: 449 3537
KasI G'GCGC_C
Cuts at: 1416 1664 1416
Size: 248 3738
KpnI G_GTAC'C
Cuts at: 1006 1006
Size: 3986
MluI A'CGCG_T
Cuts at: 3419 3419
Size: 3986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2657 2841 2657
Size: 184 3802
MscI TGG'CCA
Cuts at: 1747 1747
Size: 3986
MslI CAynn'nnrTG
Cuts at: 739 1975 2102 739
Size: 1236 127 2623
Fragments arranged by size:
2623 1236 127
MspA1I CmG'CkG
Cuts at: 303 432 599 896 929 1097 1411 1771 2784
Size: 129 167 297 33 168 314 360 1013
Cuts at: 2784 3029 303
Size: 245 1260
Fragments arranged by size:
1260 1013 360 314 297 245 168 167
129 33
NaeI GCC'GGC
Cuts at: 655 2167 655
Size: 1512 2474
NarI GG'CG_CC
Cuts at: 1417 1665 1417
Size: 248 3738
NgoMIV G'CCGG_C
Cuts at: 653 2165 653
Size: 1512 2474
NspI r_CATG'y
Cuts at: 1039 1539 2446 1039
Size: 500 907 2579
Fragments arranged by size:
2579 907 500
PasI CC'CwG_GG
Cuts at: 243 387 243
Size: 144 3842
PciI A'CATG_T
Cuts at: 2442 2442
Size: 3986
PfoI T'CCnGG_A
Cuts at: 619 619
Size: 3986
PmeI GTTT'AAAC
Cuts at: 985 985
Size: 3986
PmlI CAC'GTG
Cuts at: 486 486
Size: 3986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3954
PpuMI rG'GwC_Cy
Cuts at: 450 560 450
Size: 110 3876
PspOMI G'GGCC_C
Cuts at: 763 763
Size: 3986
PstI C_TGCA'G
Cuts at: 206 308 1033 1718 206
Size: 102 725 685 2474
Fragments arranged by size:
2474 725 685 102
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3986
PvuII CAG'CTG
Cuts at: 303 432 599 1411 1771 303
Size: 129 167 812 360 2518
Fragments arranged by size:
2518 812 360 167 129
RsrII CG'GwC_CG
Cuts at: 2181 2181
Size: 3986
SacI G_AGCT'C
Cuts at: 1000 1000
Size: 3986
SalI G'TCGA_C
Cuts at: 1023 1023
Size: 3986
SapI GCTCTTCn'nnn_
Cuts at: 2009 2219 2009
Size: 210 3776
SbfI CC_TGCA'GG
Cuts at: 1033 1033
Size: 3986
ScaI AGT'ACT
Cuts at: 3503 3503
Size: 3986
SexAI A'CCwGG_T
Cuts at: 671 671
Size: 3986
SfcI C'TryA_G
Cuts at: 33 202 304 1029 1714 2707 2898 33
Size: 169 102 725 685 993 191 1121
Fragments arranged by size:
1121 993 725 685 191 169 102
SfoI GGC'GCC
Cuts at: 1418 1666 1418
Size: 248 3738
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
SmaI CCC'GGG
Cuts at: 1008 1008
Size: 3986
SmlI C'TyrA_G
Cuts at: 378 1141 2056 2548 2810 378
Size: 763 915 492 262 1554
Fragments arranged by size:
1554 915 763 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3986
SphI G_CATG'C
Cuts at: 1039 1539 1039
Size: 500 3486
StuI AGG'CCT
Cuts at: 628 628
Size: 3986
StyI C'CwwG_G
Cuts at: 113 1120 1474 113
Size: 1007 354 2625
Fragments arranged by size:
2625 1007 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 400 570 765 1569 1702 2161 2370 400
Size: 170 195 804 133 459 209 2016
Fragments arranged by size:
2016 804 459 209 195 170 133
TatI w'GTAC_w
Cuts at: 3501 3501
Size: 3986
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 383 415 3503 3535 383
Size: 32 3088 32 834
Fragments arranged by size:
3088 834 32 32
Tth111I GACn'n_nGTC
Cuts at: 907 1783 907
Size: 876 3110
XbaI T'CTAG_A
Cuts at: 1017 1017
Size: 3986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1481 1481
Size: 3986
XmaI C'CCGG_G
Cuts at: 1006 1006
Size: 3986
XmnI GAAnn'nnTTC
Cuts at: 493 493
Size: 3986
ZraI GAC'GTC
Cuts at: 909 909
Size: 3986
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BglI BmtI BsgI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI