Restriction map for
pF1KB6343
(Circular) (Six-base) MAPSORT of: pF1KB6343.seq Check: 4569 from: 1 to: 3905
pF1KB6343 3905 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 921 921
Size: 3905
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 647 1730 2162 2909 3177 520
Size: 127 1083 432 747 268 1248
Fragments arranged by size:
1248 1083 747 432 268 127
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AlwNI CAG_nnn'CTG
Cuts at: 1210 2777 1210
Size: 1567 2338
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 321 506 520 715 909 3258 3514 321
Size: 185 14 195 194 2349 256 712
Fragments arranged by size:
2349 712 256 195 194 185 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 925 925
Size: 3905
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 863 896 863
Size: 33 3872
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 268 921 1335 1583 1618 268
Size: 653 414 248 35 2555
Fragments arranged by size:
2555 653 414 248 35
BanII G_rGCy'C
Cuts at: 919 1949 919
Size: 1030 2875
BbeI G_GCGC'C
Cuts at: 1339 1587 1339
Size: 248 3657
BbsI GAAGACnn'nnnn_
Cuts at: 899 899
Size: 3905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3871
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 1425 1425
Size: 3905
BglII A'GATC_T
Cuts at: 298 1420 3457 298
Size: 1122 2037 746
Fragments arranged by size:
2037 1122 746
BlpI GC'TnA_GC
Cuts at: 1017 1017
Size: 3905
Bme1580I G_kGCm'C
Cuts at: 1530 1623 2679 1530
Size: 93 1056 2756
Fragments arranged by size:
2756 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1117 1198 1533 3677 1117
Size: 81 335 2144 1345
Fragments arranged by size:
2144 1345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 3905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 464
Size: 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1960 2452 2750 1081
Size: 879 492 298 2236
Fragments arranged by size:
2236 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3609 3609
Size: 3905
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 825 1419 3456 6
Size: 819 594 2037 455
Fragments arranged by size:
2037 819 594 455
BsaHI Gr'CG_yC
Cuts at: 1336 1584 2286 3782 1336
Size: 248 702 1496 1459
Fragments arranged by size:
1496 1459 702 248
BsaWI w'CCGG_w
Cuts at: 585 1094 1615 2293 2567 2714 3125 585
Size: 509 521 678 274 147 411 1365
Fragments arranged by size:
1365 678 521 509 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 419 419
Size: 3905
BseYI C'CCAG_C
Cuts at: 1330 2088 2665 3161 1330
Size: 758 577 496 2074
Fragments arranged by size:
2074 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1447 1493 2701 104
Size: 1343 46 1208 1308
Fragments arranged by size:
1343 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 345 919 1697 1887 2679 345
Size: 574 778 190 792 1571
Fragments arranged by size:
1571 792 778 574 190
Bsp1286I G_dGCh'C
Cuts at: 345 919 1530 1623 1697 1887 1949 2679 345
Size: 574 611 93 74 190 62 730 1571
Fragments arranged by size:
1571 730 611 574 190 93 74 62
BspEI T'CCGG_A
Cuts at: 585 3125 585
Size: 2540 1365
BspHI T'CATG_A
Cuts at: 161 3081 161
Size: 2920 985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 955 1169 1471 1852 955
Size: 214 302 381 3008
Fragments arranged by size:
3008 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2197 2251 3735 3900 50
Size: 2147 54 1484 165 55
Fragments arranged by size:
2147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 473 585 1817 473
Size: 112 1232 2561
Fragments arranged by size:
2561 1232 112
BsrFI r'CCGG_y
Cuts at: 1903 2084 2293 1903
Size: 181 209 3515
Fragments arranged by size:
3515 209 181
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 1282 1282
Size: 3905
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstYI r'GATC_y
Cuts at: 1 298 444 753 930 1397 1420 1755 2001
Size: 297 146 309 177 467 23 335 246
Cuts at: 2001 3013 3099 3111 3457 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 309 297 246
177 146 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 815 815
Size: 3905
BtgI C'CryG_G
Cuts at: 174 264 174
Size: 90 3815
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1855 2036 2199 1855
Size: 181 163 3561
Fragments arranged by size:
3561 181 163
BtsI GCAGTG_nn'
Cuts at: 1148 1148
Size: 3905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 663 698 663
Size: 35 3870
DraI TTT'AAA
Cuts at: 720 904 720
Size: 184 3721
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 1490 1664 2055 2082 3427 1490
Size: 174 391 27 1345 1968
Fragments arranged by size:
1968 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1490 1490
Size: 3905
EarI CTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2113 2423 2569 3715 2113
Size: 310 146 1146 2303
Fragments arranged by size:
2303 1146 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 520 647 1730 2162 2909 3177 464
Size: 56 127 1083 432 747 268 1192
Fragments arranged by size:
1192 1083 747 432 268 127 56
EcoNI CCTnn'n_nnAGG
Cuts at: 804 804
Size: 3905
EcoO109I rG'GnC_Cy
Cuts at: 1044 1044
Size: 3905
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
EcoRV GAT'ATC
Cuts at: 827 827
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3905
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 1339 1587 2609 3588 1339
Size: 248 1022 979 1656
Fragments arranged by size:
1656 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1747 1777 1779 1809 1747
Size: 30 2 30 3843
Fragments arranged by size:
3843 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 1335 1583 1335
Size: 248 3657
KpnI G_GTAC'C
Cuts at: 925 925
Size: 3905
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 218 2576 2760 218
Size: 2358 184 1363
Fragments arranged by size:
2358 1363 184
MscI TGG'CCA
Cuts at: 1666 1666
Size: 3905
MslI CAynn'nnrTG
Cuts at: 179 1894 2021 179
Size: 1715 127 2063
Fragments arranged by size:
2063 1715 127
MspA1I CmG'CkG
Cuts at: 535 1016 1330 1690 2703 2948 535
Size: 481 314 360 1013 245 1492
Fragments arranged by size:
1492 1013 481 360 314 245
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 3905
NarI GG'CG_CC
Cuts at: 1336 1584 1336
Size: 248 3657
NcoI C'CATG_G
Cuts at: 174 174
Size: 3905
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 3905
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PasI CC'CwG_GG
Cuts at: 560 560
Size: 3905
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PfoI T'CCnGG_A
Cuts at: 480 480
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3873
PstI C_TGCA'G
Cuts at: 952 1637 952
Size: 685 3220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 535 1330 1690 535
Size: 795 360 2750
Fragments arranged by size:
2750 795 360
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 547 3422 547
Size: 2875 1030
SfcI C'TryA_G
Cuts at: 33 842 863 948 1633 2626 2817 33
Size: 809 21 85 685 993 191 1121
Fragments arranged by size:
1121 993 809 685 191 85 21
SfoI GGC'GCC
Cuts at: 1337 1585 1337
Size: 248 3657
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 927 927
Size: 3905
SmlI C'TyrA_G
Cuts at: 1060 1975 2467 2729 1060
Size: 915 492 262 2236
Fragments arranged by size:
2236 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
SspI AAT'ATT
Cuts at: 837 837
Size: 3905
StyI C'CwwG_G
Cuts at: 174 1039 1393 174
Size: 865 354 2686
Fragments arranged by size:
2686 865 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1488 1621 2080 2289 1488
Size: 133 459 209 3104
Fragments arranged by size:
3104 459 209 133
TatI w'GTAC_w
Cuts at: 545 3420 545
Size: 2875 1030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 429 461 3422 3454 429
Size: 32 2961 32 880
Fragments arranged by size:
2961 880 32 32
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 337 936 337
Size: 599 3306
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1400 1400
Size: 3905
XmaI C'CCGG_G
Cuts at: 925 925
Size: 3905
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI