Restriction map for
pF1KB6337
(Circular) (Six-base) MAPSORT of: pF1KB6337.seq Check: 8396 from: 1 to: 3899
pF1KB6337 3899 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 937 937
Size: 3899
Acc65I G'GTAC_C
Cuts at: 915 915
Size: 3899
AclI AA'CG_TT
Cuts at: 3739 3739
Size: 3899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 1724 2156 2903 3171 194
Size: 1530 432 747 268 922
Fragments arranged by size:
1530 922 747 432 268
AflIII A'CryG_T
Cuts at: 1251 2355 3332 1251
Size: 1104 977 1818
Fragments arranged by size:
1818 1104 977
AgeI A'CCGG_T
Cuts at: 2287 2287
Size: 3899
AleI CACnn'nnGTG
Cuts at: 581 581
Size: 3899
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 316 348 316
Size: 32 3867
AlwNI CAG_nnn'CTG
Cuts at: 1204 2771 1204
Size: 1567 2332
ApaLI G'TGCA_C
Cuts at: 2669 2669
Size: 3899
ApoI r'AATT_y
Cuts at: 903 3252 3508 903
Size: 2349 256 1294
Fragments arranged by size:
2349 1294 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3899
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
AvaI C'yCGr_G
Cuts at: 919 919
Size: 3899
BamHI G'GATC_C
Cuts at: 924 924
Size: 3899
BanI G'GyrC_C
Cuts at: 915 1329 1577 1612 915
Size: 414 248 35 3202
Fragments arranged by size:
3202 414 248 35
BanII G_rGCy'C
Cuts at: 354 913 1943 354
Size: 559 1030 2310
Fragments arranged by size:
2310 1030 559
BbeI G_GCGC'C
Cuts at: 1333 1581 1333
Size: 248 3651
BciVI GTATCCnnnnn_n'
Cuts at: 1797 2558 3899 1797
Size: 761 1341 1797
Fragments arranged by size:
1797 1341 761
BclI T'GATC_A
Cuts at: 1419 1419
Size: 3899
BglII A'GATC_T
Cuts at: 1414 3451 1414
Size: 2037 1862
BlpI GC'TnA_GC
Cuts at: 1011 1011
Size: 3899
Bme1580I G_kGCm'C
Cuts at: 1524 1617 2673 1524
Size: 93 1056 2750
Fragments arranged by size:
2750 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 303 1111 1192 1527 3671 303
Size: 808 81 335 2144 531
Fragments arranged by size:
2144 808 531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 3899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1954 2446 2744 1075
Size: 879 492 298 2230
Fragments arranged by size:
2230 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 806 3603 806
Size: 2797 1102
BsaAI yAC'GTr
Cuts at: 1252 1252
Size: 3899
BsaBI GATnn'nnATC
Cuts at: 6 653 1413 3450 6
Size: 647 760 2037 455
Fragments arranged by size:
2037 760 647 455
BsaHI Gr'CG_yC
Cuts at: 1330 1578 2280 3776 1330
Size: 248 702 1496 1453
Fragments arranged by size:
1496 1453 702 248
BsaWI w'CCGG_w
Cuts at: 1088 1609 2287 2561 2708 3119 1088
Size: 521 678 274 147 411 1868
Fragments arranged by size:
1868 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 878 908 878
Size: 30 3869
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 206 206
Size: 3899
BseYI C'CCAG_C
Cuts at: 271 429 1324 2082 2659 3155 271
Size: 158 895 758 577 496 1015
Fragments arranged by size:
1015 895 758 577 496 158
BsiEI CG_ry'CG
Cuts at: 104 1441 1487 2695 104
Size: 1337 46 1208 1308
Fragments arranged by size:
1337 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 913 1691 1881 2673 913
Size: 778 190 792 2139
Fragments arranged by size:
2139 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 354 913 1524 1617 1691 1881 1943 2673 354
Size: 559 611 93 74 190 62 730 1580
Fragments arranged by size:
1580 730 611 559 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3119 3119
Size: 3899
BspHI T'CATG_A
Cuts at: 3075 3075
Size: 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 949 1163 1465 1846 949
Size: 214 302 381 3002
Fragments arranged by size:
3002 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2191 2245 3729 3894 50
Size: 2141 54 1484 165 55
Fragments arranged by size:
2141 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 651 738 1811 651
Size: 87 1073 2739
Fragments arranged by size:
2739 1073 87
BsrFI r'CCGG_y
Cuts at: 1897 2078 2287 1897
Size: 181 209 3509
Fragments arranged by size:
3509 209 181
BssSI C'ACGA_G
Cuts at: 726 818 2170 2528 726
Size: 92 1352 358 2097
Fragments arranged by size:
2097 1352 358 92
BstAPI GCAn_nnn'nTGC
Cuts at: 1276 1276
Size: 3899
BstBI TT'CG_AA
Cuts at: 2260 2260
Size: 3899
BstEII G'GTnAC_C
Cuts at: 445 445
Size: 3899
BstYI r'GATC_y
Cuts at: 1 924 1391 1414 1749 1995 3007 3093 3105
Size: 923 467 23 335 246 1012 86 12
Cuts at: 3105 3451 1
Size: 346 449
Fragments arranged by size:
1012 923 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1849 2030 2193 1849
Size: 181 163 3555
Fragments arranged by size:
3555 181 163
BtsI GCAGTG_nn'
Cuts at: 1142 1142
Size: 3899
DraI TTT'AAA
Cuts at: 898 898
Size: 3899
DrdI GACnn_nn'nnGTC
Cuts at: 637 1605 2463 637
Size: 968 858 2073
Fragments arranged by size:
2073 968 858
EaeI y'GGCC_r
Cuts at: 1484 1658 2049 2076 3421 1484
Size: 174 391 27 1345 1962
Fragments arranged by size:
1962 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1484 1484
Size: 3899
EarI CTCTTCn'nnn_
Cuts at: 365 714 1922 2132 365
Size: 349 1208 210 2132
Fragments arranged by size:
2132 1208 349 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2107 2417 2563 3709 2107
Size: 310 146 1146 2297
Fragments arranged by size:
2297 1146 310 146
EcoICRI GAG'CTC
Cuts at: 911 911
Size: 3899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 1724 2156 2903 3171 194
Size: 1530 432 747 268 922
Fragments arranged by size:
1530 922 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 332 1038 332
Size: 706 3193
EcoRI G'AATT_C
Cuts at: 903 903
Size: 3899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 466 1918 466
Size: 1452 2447
FspI TGC'GCA
Cuts at: 1680 1680
Size: 3899
HaeII r_GCGC'y
Cuts at: 1333 1581 2603 3582 1333
Size: 248 1022 979 1650
Fragments arranged by size:
1650 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 294 326 1741 1771 1773 1803 294
Size: 32 1415 30 2 30 2390
Fragments arranged by size:
2390 1415 32 30 30 2
HincII GTy'rAC
Cuts at: 302 938 302
Size: 636 3263
KasI G'GCGC_C
Cuts at: 1329 1577 1329
Size: 248 3651
KpnI G_GTAC'C
Cuts at: 919 919
Size: 3899
MluI A'CGCG_T
Cuts at: 3332 3332
Size: 3899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2570 2754 2570
Size: 184 3715
MscI TGG'CCA
Cuts at: 1660 1660
Size: 3899
MslI CAynn'nnrTG
Cuts at: 581 1888 2015 581
Size: 1307 127 2465
Fragments arranged by size:
2465 1307 127
MspA1I CmG'CkG
Cuts at: 465 1010 1324 1684 2697 2942 465
Size: 545 314 360 1013 245 1422
Fragments arranged by size:
1422 1013 545 360 314 245
NaeI GCC'GGC
Cuts at: 2080 2080
Size: 3899
NarI GG'CG_CC
Cuts at: 1330 1578 1330
Size: 248 3651
NgoMIV G'CCGG_C
Cuts at: 2078 2078
Size: 3899
NspI r_CATG'y
Cuts at: 952 1452 2359 952
Size: 500 907 2492
Fragments arranged by size:
2492 907 500
PciI A'CATG_T
Cuts at: 2355 2355
Size: 3899
PmeI GTTT'AAAC
Cuts at: 898 898
Size: 3899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 3867
PpuMI rG'GwC_Cy
Cuts at: 332 332
Size: 3899
PstI C_TGCA'G
Cuts at: 309 946 1631 309
Size: 637 685 2577
Fragments arranged by size:
2577 685 637
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3899
PvuII CAG'CTG
Cuts at: 465 1324 1684 465
Size: 859 360 2680
Fragments arranged by size:
2680 859 360
RsrII CG'GwC_CG
Cuts at: 2094 2094
Size: 3899
SacI G_AGCT'C
Cuts at: 913 913
Size: 3899
SalI G'TCGA_C
Cuts at: 936 936
Size: 3899
SapI GCTCTTCn'nnn_
Cuts at: 365 1922 2132 365
Size: 1557 210 2132
Fragments arranged by size:
2132 1557 210
SbfI CC_TGCA'GG
Cuts at: 946 946
Size: 3899
ScaI AGT'ACT
Cuts at: 3416 3416
Size: 3899
SfcI C'TryA_G
Cuts at: 33 120 305 942 1627 2620 2811 33
Size: 87 185 637 685 993 191 1121
Fragments arranged by size:
1121 993 685 637 191 185 87
SfoI GGC'GCC
Cuts at: 1331 1579 1331
Size: 248 3651
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
SmaI CCC'GGG
Cuts at: 921 921
Size: 3899
SmlI C'TyrA_G
Cuts at: 1054 1969 2461 2723 1054
Size: 915 492 262 2230
Fragments arranged by size:
2230 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3899
SphI G_CATG'C
Cuts at: 952 1452 952
Size: 500 3399
StyI C'CwwG_G
Cuts at: 1033 1387 1033
Size: 354 3545
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 285 1482 1615 2074 2283 285
Size: 1197 133 459 209 1901
Fragments arranged by size:
1901 1197 459 209 133
TatI w'GTAC_w
Cuts at: 3414 3414
Size: 3899
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 3867
Tth111I GACn'n_nGTC
Cuts at: 1696 1696
Size: 3899
XbaI T'CTAG_A
Cuts at: 930 930
Size: 3899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 343 1394 343
Size: 1051 2848
XmaI C'CCGG_G
Cuts at: 919 919
Size: 3899
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI