Restriction map for
pF1KB6334
(Circular) (Six-base) MAPSORT of: pF1KB6334.seq Check: 7013 from: 1 to: 3980
pF1KB6334 3980 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1018 1018
Size: 3980
Acc65I G'GTAC_C
Cuts at: 996 996
Size: 3980
AclI AA'CG_TT
Cuts at: 3820 3820
Size: 3980
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 412 1805 2237 2984 3252 151
Size: 261 1393 432 747 268 879
Fragments arranged by size:
1393 879 747 432 268 261
AflIII A'CryG_T
Cuts at: 261 746 1332 2436 3413 261
Size: 485 586 1104 977 828
Fragments arranged by size:
1104 977 828 586 485
AgeI A'CCGG_T
Cuts at: 2368 2368
Size: 3980
AlwNI CAG_nnn'CTG
Cuts at: 1285 2852 1285
Size: 1567 2413
ApaLI G'TGCA_C
Cuts at: 2750 2750
Size: 3980
ApoI r'AATT_y
Cuts at: 984 3333 3589 984
Size: 2349 256 1375
Fragments arranged by size:
2349 1375 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3980
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
AvaI C'yCGr_G
Cuts at: 1000 1000
Size: 3980
BamHI G'GATC_C
Cuts at: 1005 1005
Size: 3980
BanI G'GyrC_C
Cuts at: 996 1410 1658 1693 996
Size: 414 248 35 3283
Fragments arranged by size:
3283 414 248 35
BanII G_rGCy'C
Cuts at: 994 2024 994
Size: 1030 2950
BbeI G_GCGC'C
Cuts at: 1414 1662 1414
Size: 248 3732
BciVI GTATCCnnnnn_n'
Cuts at: 1878 2639 3980 1878
Size: 761 1341 1878
Fragments arranged by size:
1878 1341 761
BclI T'GATC_A
Cuts at: 1500 1500
Size: 3980
BglII A'GATC_T
Cuts at: 251 1495 3532 251
Size: 1244 2037 699
Fragments arranged by size:
2037 1244 699
BlpI GC'TnA_GC
Cuts at: 1092 1092
Size: 3980
Bme1580I G_kGCm'C
Cuts at: 1605 1698 2754 1605
Size: 93 1056 2831
Fragments arranged by size:
2831 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1192 1273 1608 3752 1192
Size: 81 335 2144 1420
Fragments arranged by size:
2144 1420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 3980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 622 739 142
Size: 480 117 3383
Fragments arranged by size:
3383 480 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 1156 2035 2527 2825 503
Size: 653 879 492 298 1658
Fragments arranged by size:
1658 879 653 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3684 3684
Size: 3980
BsaAI yAC'GTr
Cuts at: 834 1333 834
Size: 499 3481
BsaBI GATnn'nnATC
Cuts at: 6 181 904 1494 3531 6
Size: 175 723 590 2037 455
Fragments arranged by size:
2037 723 590 455 175
BsaHI Gr'CG_yC
Cuts at: 1411 1659 2361 3857 1411
Size: 248 702 1496 1534
Fragments arranged by size:
1534 1496 702 248
BsaWI w'CCGG_w
Cuts at: 380 1169 1690 2368 2642 2789 3200 380
Size: 789 521 678 274 147 411 1160
Fragments arranged by size:
1160 789 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 595 625 595
Size: 30 3950
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 283 610 619 283
Size: 327 9 3644
Fragments arranged by size:
3644 327 9
BseYI C'CCAG_C
Cuts at: 461 930 1405 2163 2740 3236 461
Size: 469 475 758 577 496 1205
Fragments arranged by size:
1205 758 577 496 475 469
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 287
Size: 3980
BsiEI CG_ry'CG
Cuts at: 104 1522 1568 2776 104
Size: 1418 46 1208 1308
Fragments arranged by size:
1418 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 994 1772 1962 2754 994
Size: 778 190 792 2220
Fragments arranged by size:
2220 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 994 1605 1698 1772 1962 2024 2754 994
Size: 611 93 74 190 62 730 2220
Fragments arranged by size:
2220 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 380 3200 380
Size: 2820 1160
BspHI T'CATG_A
Cuts at: 3156 3156
Size: 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1030 1244 1546 1927 1030
Size: 214 302 381 3083
Fragments arranged by size:
3083 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2272 2326 3810 3975 50
Size: 2222 54 1484 165 55
Fragments arranged by size:
2222 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1892 1892
Size: 3980
BsrFI r'CCGG_y
Cuts at: 1978 2159 2368 1978
Size: 181 209 3590
Fragments arranged by size:
3590 209 181
BssSI C'ACGA_G
Cuts at: 845 2251 2609 845
Size: 1406 358 2216
Fragments arranged by size:
2216 1406 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1357 1357
Size: 3980
BstBI TT'CG_AA
Cuts at: 2341 2341
Size: 3980
BstXI CCAn_nnnn'nTGG
Cuts at: 771 771
Size: 3980
BstYI r'GATC_y
Cuts at: 1 251 1005 1472 1495 1830 2076 3088 3174
Size: 250 754 467 23 335 246 1012 86
Cuts at: 3174 3186 3532 1
Size: 12 346 449
Fragments arranged by size:
1012 754 467 449 346 335 250 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 744 1930 2111 2274 744
Size: 1186 181 163 2450
Fragments arranged by size:
2450 1186 181 163
BtsI GCAGTG_nn'
Cuts at: 1223 1223
Size: 3980
DraI TTT'AAA
Cuts at: 979 979
Size: 3980
DraIII CAC_nnn'GTG
Cuts at: 825 825
Size: 3980
DrdI GACnn_nn'nnGTC
Cuts at: 1686 2544 1686
Size: 858 3122
EaeI y'GGCC_r
Cuts at: 629 1565 1739 2130 2157 3502 629
Size: 936 174 391 27 1345 1107
Fragments arranged by size:
1345 1107 936 391 174 27
EagI C'GGCC_G
Cuts at: 1565 1565
Size: 3980
EarI CTCTTCn'nnn_
Cuts at: 388 2003 2213 388
Size: 1615 210 2155
Fragments arranged by size:
2155 1615 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2188 2498 2644 3790 2188
Size: 310 146 1146 2378
Fragments arranged by size:
2378 1146 310 146
EcoICRI GAG'CTC
Cuts at: 992 992
Size: 3980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 151 412 622 739 1805 2237 2984 3252
Size: 9 261 210 117 1066 432 747 268
Cuts at: 3252 142
Size: 870
Fragments arranged by size:
1066 870 747 432 268 261 210 117
9
EcoO109I rG'GnC_Cy
Cuts at: 1119 1119
Size: 3980
EcoRI G'AATT_C
Cuts at: 984 984
Size: 3980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3980
FspI TGC'GCA
Cuts at: 1761 1761
Size: 3980
HaeII r_GCGC'y
Cuts at: 1414 1662 2684 3663 1414
Size: 248 1022 979 1731
Fragments arranged by size:
1731 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 415 447 1822 1852 1854 1884 238
Size: 32 145 32 1375 30 2 30 2334
Fragments arranged by size:
2334 1375 145 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1019 1019
Size: 3980
KasI G'GCGC_C
Cuts at: 1410 1658 1410
Size: 248 3732
KpnI G_GTAC'C
Cuts at: 1000 1000
Size: 3980
MluI A'CGCG_T
Cuts at: 3413 3413
Size: 3980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 593 2651 2835 593
Size: 2058 184 1738
Fragments arranged by size:
2058 1738 184
MscI TGG'CCA
Cuts at: 1741 1741
Size: 3980
MslI CAynn'nnrTG
Cuts at: 751 763 769 1969 2096 751
Size: 12 6 1200 127 2635
Fragments arranged by size:
2635 1200 127 12 6
MspA1I CmG'CkG
Cuts at: 129 144 558 1091 1405 1765 2778 3023 129
Size: 15 414 533 314 360 1013 245 1086
Fragments arranged by size:
1086 1013 533 414 360 314 245 15
NaeI GCC'GGC
Cuts at: 2161 2161
Size: 3980
NarI GG'CG_CC
Cuts at: 1411 1659 1411
Size: 248 3732
NgoMIV G'CCGG_C
Cuts at: 2159 2159
Size: 3980
NspI r_CATG'y
Cuts at: 265 750 1033 1533 2440 265
Size: 485 283 500 907 1805
Fragments arranged by size:
1805 907 500 485 283
PciI A'CATG_T
Cuts at: 261 746 2436 261
Size: 485 1690 1805
Fragments arranged by size:
1805 1690 485
PmeI GTTT'AAAC
Cuts at: 979 979
Size: 3980
PmlI CAC'GTG
Cuts at: 834 834
Size: 3980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 3948
PstI C_TGCA'G
Cuts at: 1027 1712 1027
Size: 685 3295
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3980
PvuII CAG'CTG
Cuts at: 129 144 1405 1765 129
Size: 15 1261 360 2344
Fragments arranged by size:
2344 1261 360 15
RsrII CG'GwC_CG
Cuts at: 2175 2175
Size: 3980
SacI G_AGCT'C
Cuts at: 994 994
Size: 3980
SalI G'TCGA_C
Cuts at: 1017 1017
Size: 3980
SapI GCTCTTCn'nnn_
Cuts at: 388 2003 2213 388
Size: 1615 210 2155
Fragments arranged by size:
2155 1615 210
SbfI CC_TGCA'GG
Cuts at: 1027 1027
Size: 3980
ScaI AGT'ACT
Cuts at: 3497 3497
Size: 3980
SfcI C'TryA_G
Cuts at: 33 285 1023 1708 2701 2892 33
Size: 252 738 685 993 191 1121
Fragments arranged by size:
1121 993 738 685 252 191
SfoI GGC'GCC
Cuts at: 1412 1660 1412
Size: 248 3732
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
SmaI CCC'GGG
Cuts at: 1002 1002
Size: 3980
SmlI C'TyrA_G
Cuts at: 518 1135 2050 2542 2804 518
Size: 617 915 492 262 1694
Fragments arranged by size:
1694 915 617 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3980
SphI G_CATG'C
Cuts at: 1033 1533 1033
Size: 500 3480
StyI C'CwwG_G
Cuts at: 326 1114 1468 326
Size: 788 354 2838
Fragments arranged by size:
2838 788 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1563 1696 2155 2364 1563
Size: 133 459 209 3179
Fragments arranged by size:
3179 459 209 133
TatI w'GTAC_w
Cuts at: 3495 3495
Size: 3980
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 3948
Tth111I GACn'n_nGTC
Cuts at: 1777 1777
Size: 3980
XbaI T'CTAG_A
Cuts at: 1011 1011
Size: 3980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1475 1475
Size: 3980
XmaI C'CCGG_G
Cuts at: 1000 1000
Size: 3980
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI