Restriction map for
pF1KB6327
(Circular) (Six-base) MAPSORT of: pF1KB6327.seq Check: 3694 from: 1 to: 3896
pF1KB6327 3896 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 725 912 725
Size: 187 3709
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2153 2900 3168 1721
Size: 432 747 268 2449
Fragments arranged by size:
2449 747 432 268
AflIII A'CryG_T
Cuts at: 1248 2352 3329 1248
Size: 1104 977 1815
Fragments arranged by size:
1815 1104 977
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AleI CACnn'nnGTG
Cuts at: 298 298
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 1201 2768 1201
Size: 1567 2329
ApaI G_GGCC'C
Cuts at: 505 505
Size: 3896
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 421 916 421
Size: 495 3401
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 725 912 1326 1574 1609 725
Size: 187 414 248 35 3012
Fragments arranged by size:
3012 414 248 187 35
BanII G_rGCy'C
Cuts at: 347 428 505 910 1940 347
Size: 81 77 405 1030 2303
Fragments arranged by size:
2303 1030 405 81 77
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BbsI GAAGACnn'nnnn_
Cuts at: 448 448
Size: 3896
BciVI GTATCCnnnnn_n'
Cuts at: 155 1794 2555 3896 155
Size: 1639 761 1341 155
Fragments arranged by size:
1639 1341 761 155
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglI GCCn_nnn'nGGC
Cuts at: 500 500
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 505 612 1521 1614 2670 505
Size: 107 909 93 1056 1731
Fragments arranged by size:
1731 1056 909 107 93
BmrI ACTGGGnnnn_n'
Cuts at: 642 676 1108 1189 1524 3668 642
Size: 34 432 81 335 2144 870
Fragments arranged by size:
2144 870 432 335 81 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 412 595 331
Size: 81 183 3632
Fragments arranged by size:
3632 183 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 1072 1951 2443 2741 313
Size: 759 879 492 298 1468
Fragments arranged by size:
1468 879 759 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 199 774 3600 199
Size: 575 2826 495
Fragments arranged by size:
2826 575 495
BsaAI yAC'GTr
Cuts at: 879 1249 879
Size: 370 3526
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 1327 1575 2277 3773 1327
Size: 248 702 1496 1450
Fragments arranged by size:
1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 754 784 754
Size: 30 3866
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 493 385
Size: 108 3788
BseYI C'CCAG_C
Cuts at: 629 1321 2079 2656 3152 629
Size: 692 758 577 496 1373
Fragments arranged by size:
1373 758 692 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 823
Size: 3896
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 347 428 910 1688 1878 2670 347
Size: 81 482 778 190 792 1573
Fragments arranged by size:
1573 792 778 482 190 81
Bsp1286I G_dGCh'C
Cuts at: 347 428 505 612 910 1521 1614 1688 1878
Size: 81 77 107 298 611 93 74 190
Cuts at: 1878 1940 2670 347
Size: 62 730 1573
Fragments arranged by size:
1573 730 611 298 190 107 93 81
77 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 217 946 1160 1462 1843 217
Size: 729 214 302 381 2270
Fragments arranged by size:
2270 729 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 198 1808 198
Size: 1610 2286
BsrFI r'CCGG_y
Cuts at: 563 639 847 867 1894 2075 2284 563
Size: 76 208 20 1027 181 209 2175
Fragments arranged by size:
2175 1027 209 208 181 76 20
BssSI C'ACGA_G
Cuts at: 2167 2525 2167
Size: 358 3538
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 655 879 655
Size: 224 3672
BstYI r'GATC_y
Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102
Size: 920 467 23 335 246 1012 86 12
Cuts at: 3102 3448 1
Size: 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 149 296 149
Size: 147 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 1139 1139
Size: 3896
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 793 1602 2460 793
Size: 809 858 2229
Fragments arranged by size:
2229 858 809
EaeI y'GGCC_r
Cuts at: 869 1481 1655 2046 2073 3418 869
Size: 612 174 391 27 1345 1347
Fragments arranged by size:
1347 1345 612 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 484 1919 2129 484
Size: 1435 210 2251
Fragments arranged by size:
2251 1435 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 319 2104 2414 2560 3706 319
Size: 1785 310 146 1146 509
Fragments arranged by size:
1785 1146 509 310 146
EcoICRI GAG'CTC
Cuts at: 345 426 908 345
Size: 81 482 3333
Fragments arranged by size:
3333 482 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 412 595 1721 2153 2900 3168 331
Size: 81 183 1126 432 747 268 1059
Fragments arranged by size:
1126 1059 747 432 268 183 81
EcoO109I rG'GnC_Cy
Cuts at: 502 1035 502
Size: 533 3363
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 306 1915 306
Size: 1609 2287
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 704 752 1330 1578 2600 3579 704
Size: 48 578 248 1022 979 1021
Fragments arranged by size:
1022 1021 979 578 248 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 129 161 754 786 1738 1768 1770 1800 129
Size: 32 593 32 952 30 2 30 2225
Fragments arranged by size:
2225 952 593 32 32 30 30 2
HincII GTy'rAC
Cuts at: 798 935 798
Size: 137 3759
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 729 916 729
Size: 187 3709
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 298 859 1885 2012 298
Size: 561 1026 127 2182
Fragments arranged by size:
2182 1026 561 127
MspA1I CmG'CkG
Cuts at: 1007 1321 1681 2694 2939 1007
Size: 314 360 1013 245 1964
Fragments arranged by size:
1964 1013 360 314 245
NaeI GCC'GGC
Cuts at: 869 2077 869
Size: 1208 2688
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 296 296
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 867 2075 867
Size: 1208 2688
NspI r_CATG'y
Cuts at: 194 949 1449 2356 194
Size: 755 500 907 1734
Fragments arranged by size:
1734 907 755 500
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PflMI CCAn_nnn'nTGG
Cuts at: 712 712
Size: 3896
PfoI T'CCnGG_A
Cuts at: 776 776
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PspOMI G'GGCC_C
Cuts at: 501 501
Size: 3896
PstI C_TGCA'G
Cuts at: 678 943 1628 678
Size: 265 685 2946
Fragments arranged by size:
2946 685 265
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 1321 1681 1321
Size: 360 3536
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 347 428 910 347
Size: 81 482 3333
Fragments arranged by size:
3333 482 81
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 484 1919 2129 484
Size: 1435 210 2251
Fragments arranged by size:
2251 1435 210
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 674 744 939 1624 2617 2808 33
Size: 641 70 195 685 993 191 1121
Fragments arranged by size:
1121 993 685 641 195 191 70
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SgrAI Cr'CCGG_yG
Cuts at: 847 847
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 328 1051 1966 2458 2720 328
Size: 723 915 492 262 1504
Fragments arranged by size:
1504 915 723 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 194 949 1449 194
Size: 755 500 2641
Fragments arranged by size:
2641 755 500
StyI C'CwwG_G
Cuts at: 296 1030 1384 296
Size: 734 354 2808
Fragments arranged by size:
2808 734 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1479 1612 2071 2280 1479
Size: 133 459 209 3095
Fragments arranged by size:
3095 459 209 133
TatI w'GTAC_w
Cuts at: 3411 3411
Size: 3896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 766 1693 766
Size: 927 2969
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 655 1391 655
Size: 736 3160
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
XmnI GAAnn'nnTTC
Cuts at: 320 320
Size: 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI ZraI