Restriction map for
pF1KB6319
(Circular) (Six-base) MAPSORT of: pF1KB6319.seq Check: 9919 from: 1 to: 3893
pF1KB6319 3893 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 303 303
Size: 3893
AccI GT'mk_AC
Cuts at: 931 931
Size: 3893
Acc65I G'GTAC_C
Cuts at: 909 909
Size: 3893
AclI AA'CG_TT
Cuts at: 3733 3733
Size: 3893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1718 2150 2897 3165 1718
Size: 432 747 268 2446
Fragments arranged by size:
2446 747 432 268
AflIII A'CryG_T
Cuts at: 1245 2349 3326 1245
Size: 1104 977 1812
Fragments arranged by size:
1812 1104 977
AgeI A'CCGG_T
Cuts at: 2281 2281
Size: 3893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 507 507
Size: 3893
AlwNI CAG_nnn'CTG
Cuts at: 604 1198 2765 604
Size: 594 1567 1732
Fragments arranged by size:
1732 1567 594
ApaI G_GGCC'C
Cuts at: 301 301
Size: 3893
ApaLI G'TGCA_C
Cuts at: 2663 2663
Size: 3893
ApoI r'AATT_y
Cuts at: 508 897 3246 3502 508
Size: 389 2349 256 899
Fragments arranged by size:
2349 899 389 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3893
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
AvaI C'yCGr_G
Cuts at: 134 913 134
Size: 779 3114
BamHI G'GATC_C
Cuts at: 918 918
Size: 3893
BanI G'GyrC_C
Cuts at: 277 909 1323 1571 1606 277
Size: 632 414 248 35 2564
Fragments arranged by size:
2564 632 414 248 35
BanII G_rGCy'C
Cuts at: 301 907 1937 301
Size: 606 1030 2257
Fragments arranged by size:
2257 1030 606
BbeI G_GCGC'C
Cuts at: 1327 1575 1327
Size: 248 3645
BciVI GTATCCnnnnn_n'
Cuts at: 1791 2552 3893 1791
Size: 761 1341 1791
Fragments arranged by size:
1791 1341 761
BclI T'GATC_A
Cuts at: 756 1413 756
Size: 657 3236
BglII A'GATC_T
Cuts at: 1408 3445 1408
Size: 2037 1856
BlpI GC'TnA_GC
Cuts at: 1005 1005
Size: 3893
Bme1580I G_kGCm'C
Cuts at: 301 1518 1611 2667 301
Size: 1217 93 1056 1527
Fragments arranged by size:
1527 1217 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 576 1105 1186 1521 3665 576
Size: 529 81 335 2144 804
Fragments arranged by size:
2144 804 529 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 3893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 581 388
Size: 193 3700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 762 1069 1948 2440 2738 650
Size: 112 307 879 492 298 1805
Fragments arranged by size:
1805 879 492 307 298 112
BsaI GGTCTCn'nnnn_
Cuts at: 3597 3597
Size: 3893
BsaAI yAC'GTr
Cuts at: 1246 1246
Size: 3893
BsaBI GATnn'nnATC
Cuts at: 6 1407 3444 6
Size: 1401 2037 455
Fragments arranged by size:
2037 1401 455
BsaHI Gr'CG_yC
Cuts at: 1324 1572 2274 3770 1324
Size: 248 702 1496 1447
Fragments arranged by size:
1496 1447 702 248
BsaWI w'CCGG_w
Cuts at: 167 1082 1603 2281 2555 2702 3113 167
Size: 915 521 678 274 147 411 947
Fragments arranged by size:
947 915 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 214 244 214
Size: 30 3863
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 222 222
Size: 3893
BseYI C'CCAG_C
Cuts at: 1318 2076 2653 3149 1318
Size: 758 577 496 2062
Fragments arranged by size:
2062 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 439
Size: 3893
BsiEI CG_ry'CG
Cuts at: 104 1435 1481 2689 104
Size: 1331 46 1208 1308
Fragments arranged by size:
1331 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 907 1685 1875 2667 907
Size: 778 190 792 2133
Fragments arranged by size:
2133 792 778 190
BsmI GAATG_Cn'
Cuts at: 654 654
Size: 3893
BsmBI CGTCTCn'nnnn_
Cuts at: 196 196
Size: 3893
Bsp1286I G_dGCh'C
Cuts at: 301 907 1518 1611 1685 1875 1937 2667 301
Size: 606 611 93 74 190 62 730 1527
Fragments arranged by size:
1527 730 611 606 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3113 3113
Size: 3893
BspHI T'CATG_A
Cuts at: 3069 3069
Size: 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 303 943 1157 1459 1840 303
Size: 640 214 302 381 2356
Fragments arranged by size:
2356 640 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2185 2239 3723 3888 50
Size: 2135 54 1484 165 55
Fragments arranged by size:
2135 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1805 1805
Size: 3893
BsrFI r'CCGG_y
Cuts at: 1891 2072 2281 1891
Size: 181 209 3503
Fragments arranged by size:
3503 209 181
BsrGI T'GTAC_A
Cuts at: 792 792
Size: 3893
BssSI C'ACGA_G
Cuts at: 2164 2522 2164
Size: 358 3535
BstAPI GCAn_nnn'nTGC
Cuts at: 417 1270 417
Size: 853 3040
BstBI TT'CG_AA
Cuts at: 2254 2254
Size: 3893
BstYI r'GATC_y
Cuts at: 1 918 1385 1408 1743 1989 3001 3087 3099
Size: 917 467 23 335 246 1012 86 12
Cuts at: 3099 3445 1
Size: 346 449
Fragments arranged by size:
1012 917 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 192 696 192
Size: 504 3389
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1843 2024 2187 1843
Size: 181 163 3549
Fragments arranged by size:
3549 181 163
BtsI GCAGTG_nn'
Cuts at: 1136 1136
Size: 3893
DraI TTT'AAA
Cuts at: 892 892
Size: 3893
DrdI GACnn_nn'nnGTC
Cuts at: 1599 2457 1599
Size: 858 3035
EaeI y'GGCC_r
Cuts at: 687 1478 1652 2043 2070 3415 687
Size: 791 174 391 27 1345 1165
Fragments arranged by size:
1345 1165 791 391 174 27
EagI C'GGCC_G
Cuts at: 1478 1478
Size: 3893
EarI CTCTTCn'nnn_
Cuts at: 865 1916 2126 865
Size: 1051 210 2632
Fragments arranged by size:
2632 1051 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2101 2411 2557 3703 2101
Size: 310 146 1146 2291
Fragments arranged by size:
2291 1146 310 146
EcoICRI GAG'CTC
Cuts at: 905 905
Size: 3893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 581 1718 2150 2897 3165 388
Size: 193 1137 432 747 268 1116
Fragments arranged by size:
1137 1116 747 432 268 193
EcoO109I rG'GnC_Cy
Cuts at: 298 1032 298
Size: 734 3159
EcoRI G'AATT_C
Cuts at: 897 897
Size: 3893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 649 1912 649
Size: 1263 2630
FspI TGC'GCA
Cuts at: 1674 1674
Size: 3893
HaeII r_GCGC'y
Cuts at: 1327 1575 2597 3576 1327
Size: 248 1022 979 1644
Fragments arranged by size:
1644 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 357 389 748 780 1735 1765 1767 1797 357
Size: 32 359 32 955 30 2 30 2453
Fragments arranged by size:
2453 955 359 32 32 30 30 2
HincII GTy'rAC
Cuts at: 932 932
Size: 3893
KasI G'GCGC_C
Cuts at: 1323 1571 1323
Size: 248 3645
KpnI G_GTAC'C
Cuts at: 913 913
Size: 3893
MluI A'CGCG_T
Cuts at: 3326 3326
Size: 3893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 154 2564 2748 154
Size: 2410 184 1299
Fragments arranged by size:
2410 1299 184
MscI TGG'CCA
Cuts at: 689 1654 689
Size: 965 2928
MslI CAynn'nnrTG
Cuts at: 1882 2009 1882
Size: 127 3766
MspA1I CmG'CkG
Cuts at: 1004 1318 1678 2691 2936 1004
Size: 314 360 1013 245 1961
Fragments arranged by size:
1961 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2074 2074
Size: 3893
NarI GG'CG_CC
Cuts at: 1324 1572 1324
Size: 248 3645
NgoMIV G'CCGG_C
Cuts at: 2072 2072
Size: 3893
NspI r_CATG'y
Cuts at: 826 946 1446 2353 826
Size: 120 500 907 2366
Fragments arranged by size:
2366 907 500 120
PasI CC'CwG_GG
Cuts at: 158 158
Size: 3893
PciI A'CATG_T
Cuts at: 2349 2349
Size: 3893
PmeI GTTT'AAAC
Cuts at: 892 892
Size: 3893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3032 3064 3032
Size: 32 3861
PsiI TTA'TAA
Cuts at: 844 844
Size: 3893
PspOMI G'GGCC_C
Cuts at: 297 297
Size: 3893
PstI C_TGCA'G
Cuts at: 940 1625 940
Size: 685 3208
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3893
PvuII CAG'CTG
Cuts at: 1318 1678 1318
Size: 360 3533
RsrII CG'GwC_CG
Cuts at: 2088 2088
Size: 3893
SacI G_AGCT'C
Cuts at: 907 907
Size: 3893
SalI G'TCGA_C
Cuts at: 930 930
Size: 3893
SapI GCTCTTCn'nnn_
Cuts at: 1916 2126 1916
Size: 210 3683
SbfI CC_TGCA'GG
Cuts at: 940 940
Size: 3893
ScaI AGT'ACT
Cuts at: 3410 3410
Size: 3893
SfcI C'TryA_G
Cuts at: 33 936 1621 2614 2805 33
Size: 903 685 993 191 1121
Fragments arranged by size:
1121 993 903 685 191
SfoI GGC'GCC
Cuts at: 1325 1573 1325
Size: 248 3645
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
SmaI CCC'GGG
Cuts at: 915 915
Size: 3893
SmlI C'TyrA_G
Cuts at: 665 777 1048 1963 2455 2717 665
Size: 112 271 915 492 262 1841
Fragments arranged by size:
1841 915 492 271 262 112
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3893
SphI G_CATG'C
Cuts at: 946 1446 946
Size: 500 3393
SspI AAT'ATT
Cuts at: 528 528
Size: 3893
StyI C'CwwG_G
Cuts at: 338 1027 1381 338
Size: 689 354 2850
Fragments arranged by size:
2850 689 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1476 1609 2068 2277 1476
Size: 133 459 209 3092
Fragments arranged by size:
3092 459 209 133
TatI w'GTAC_w
Cuts at: 792 3408 792
Size: 2616 1277
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 214 246 751 783 3410 3442 214
Size: 32 505 32 2627 32 665
Fragments arranged by size:
2627 665 505 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1690 1690
Size: 3893
XbaI T'CTAG_A
Cuts at: 381 924 381
Size: 543 3350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1388 1388
Size: 3893
XmaI C'CCGG_G
Cuts at: 913 913
Size: 3893
XmnI GAAnn'nnTTC
Cuts at: 152 152
Size: 3893
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PmeI PpiI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI