Restriction map for
pF1KB6318
(Circular) (Six-base) MAPSORT of: pF1KB6318.seq Check: 1701 from: 1 to: 3956
pF1KB6318 3956 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 994 994
Size: 3956
Acc65I G'GTAC_C
Cuts at: 972 972
Size: 3956
AclI AA'CG_TT
Cuts at: 3796 3796
Size: 3956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 571 1781 2213 2960 3228 386
Size: 185 1210 432 747 268 1114
Fragments arranged by size:
1210 1114 747 432 268 185
AflIII A'CryG_T
Cuts at: 1308 2412 3389 1308
Size: 1104 977 1875
Fragments arranged by size:
1875 1104 977
AgeI A'CCGG_T
Cuts at: 2344 2344
Size: 3956
AlwNI CAG_nnn'CTG
Cuts at: 844 1261 2828 844
Size: 417 1567 1972
Fragments arranged by size:
1972 1567 417
ApaLI G'TGCA_C
Cuts at: 2726 2726
Size: 3956
ApoI r'AATT_y
Cuts at: 545 621 960 3309 3565 545
Size: 76 339 2349 256 936
Fragments arranged by size:
2349 936 339 256 76
AseI AT'TA_AT
Cuts at: 20 377 688 938 20
Size: 357 311 250 3038
Fragments arranged by size:
3038 357 311 250
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
AvaI C'yCGr_G
Cuts at: 203 286 976 203
Size: 83 690 3183
Fragments arranged by size:
3183 690 83
BamHI G'GATC_C
Cuts at: 981 981
Size: 3956
BanI G'GyrC_C
Cuts at: 972 1386 1634 1669 972
Size: 414 248 35 3259
Fragments arranged by size:
3259 414 248 35
BanII G_rGCy'C
Cuts at: 161 320 970 2000 161
Size: 159 650 1030 2117
Fragments arranged by size:
2117 1030 650 159
BbeI G_GCGC'C
Cuts at: 1390 1638 1390
Size: 248 3708
BbsI GAAGACnn'nnnn_
Cuts at: 215 215
Size: 3956
BciVI GTATCCnnnnn_n'
Cuts at: 503 926 1854 2615 3956 503
Size: 423 928 761 1341 503
Fragments arranged by size:
1341 928 761 503 423
BclI T'GATC_A
Cuts at: 1476 1476
Size: 3956
BglII A'GATC_T
Cuts at: 1471 3508 1471
Size: 2037 1919
BlpI GC'TnA_GC
Cuts at: 1068 1068
Size: 3956
Bme1580I G_kGCm'C
Cuts at: 1581 1674 2730 1581
Size: 93 1056 2807
Fragments arranged by size:
2807 1056 93
BmgBI CAC'GTC
Cuts at: 867 867
Size: 3956
BmrI ACTGGGnnnn_n'
Cuts at: 1168 1249 1584 3728 1168
Size: 81 335 2144 1396
Fragments arranged by size:
2144 1396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 2323
Size: 3956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 3956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 2011 2503 2801 1132
Size: 879 492 298 2287
Fragments arranged by size:
2287 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 195 3660 195
Size: 3465 491
BsaAI yAC'GTr
Cuts at: 1309 1309
Size: 3956
BsaBI GATnn'nnATC
Cuts at: 6 1470 3507 6
Size: 1464 2037 455
Fragments arranged by size:
2037 1464 455
BsaHI Gr'CG_yC
Cuts at: 1387 1635 2337 3833 1387
Size: 248 702 1496 1510
Fragments arranged by size:
1510 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1145 1666 2344 2618 2765 3176 1145
Size: 521 678 274 147 411 1925
Fragments arranged by size:
1925 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 319
Size: 3956
BseYI C'CCAG_C
Cuts at: 1381 2139 2716 3212 1381
Size: 758 577 496 2125
Fragments arranged by size:
2125 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 210
Size: 3956
BsiEI CG_ry'CG
Cuts at: 104 270 1498 1544 2752 104
Size: 166 1228 46 1208 1308
Fragments arranged by size:
1308 1228 1208 166 46
BsiHKAI G_wGCw'C
Cuts at: 161 970 1748 1938 2730 161
Size: 809 778 190 792 1387
Fragments arranged by size:
1387 809 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 161 320 970 1581 1674 1748 1938 2000 2730
Size: 159 650 611 93 74 190 62 730
Cuts at: 2730 161
Size: 1387
Fragments arranged by size:
1387 730 650 611 190 159 93 74
62
BspEI T'CCGG_A
Cuts at: 3176 3176
Size: 3956
BspHI T'CATG_A
Cuts at: 3132 3132
Size: 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1006 1220 1522 1903 1006
Size: 214 302 381 3059
Fragments arranged by size:
3059 381 302 214
BsrBI CCG'CTC
Cuts at: 50 322 2248 2302 3786 3951 50
Size: 272 1926 54 1484 165 55
Fragments arranged by size:
1926 1484 272 165 55 54
BsrDI GCAATG_nn'
Cuts at: 919 1868 919
Size: 949 3007
BsrFI r'CCGG_y
Cuts at: 1954 2135 2344 1954
Size: 181 209 3566
Fragments arranged by size:
3566 209 181
BsrGI T'GTAC_A
Cuts at: 570 570
Size: 3956
BssSI C'ACGA_G
Cuts at: 2227 2585 2227
Size: 358 3598
BstAPI GCAn_nnn'nTGC
Cuts at: 1333 1333
Size: 3956
BstBI TT'CG_AA
Cuts at: 2317 2317
Size: 3956
BstYI r'GATC_y
Cuts at: 1 503 981 1448 1471 1806 2052 3064 3150
Size: 502 478 467 23 335 246 1012 86
Cuts at: 3150 3162 3508 1
Size: 12 346 449
Fragments arranged by size:
1012 502 478 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 145 107
Size: 38 3918
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1906 2087 2250 1906
Size: 181 163 3612
Fragments arranged by size:
3612 181 163
BtsI GCAGTG_nn'
Cuts at: 343 1199 343
Size: 856 3100
DraI TTT'AAA
Cuts at: 955 955
Size: 3956
DrdI GACnn_nn'nnGTC
Cuts at: 1662 2520 1662
Size: 858 3098
EaeI y'GGCC_r
Cuts at: 267 925 1541 1715 2106 2133 3478 267
Size: 658 616 174 391 27 1345 745
Fragments arranged by size:
1345 745 658 616 391 174 27
EagI C'GGCC_G
Cuts at: 267 1541 267
Size: 1274 2682
EarI CTCTTCn'nnn_
Cuts at: 1979 2189 1979
Size: 210 3746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 183 2164 2474 2620 3766 183
Size: 1981 310 146 1146 373
Fragments arranged by size:
1981 1146 373 310 146
EcoICRI GAG'CTC
Cuts at: 159 968 159
Size: 809 3147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 571 730 1781 2213 2960 3228 386
Size: 185 159 1051 432 747 268 1114
Fragments arranged by size:
1114 1051 747 432 268 185 159
EcoO109I rG'GnC_Cy
Cuts at: 1095 1095
Size: 3956
EcoRI G'AATT_C
Cuts at: 960 960
Size: 3956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1975 1975
Size: 3956
FspI TGC'GCA
Cuts at: 335 1737 335
Size: 1402 2554
HaeII r_GCGC'y
Cuts at: 1390 1638 2660 3639 1390
Size: 248 1022 979 1707
Fragments arranged by size:
1707 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1798 1828 1830 1860 1798
Size: 30 2 30 3894
Fragments arranged by size:
3894 30 30 2
HincII GTy'rAC
Cuts at: 995 995
Size: 3956
HindIII A'AGCT_T
Cuts at: 398 398
Size: 3956
KasI G'GCGC_C
Cuts at: 1386 1634 1386
Size: 248 3708
KpnI G_GTAC'C
Cuts at: 976 976
Size: 3956
MluI A'CGCG_T
Cuts at: 3389 3389
Size: 3956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2811 2627
Size: 184 3772
MscI TGG'CCA
Cuts at: 1717 1717
Size: 3956
MslI CAynn'nnrTG
Cuts at: 1945 2072 1945
Size: 127 3829
MspA1I CmG'CkG
Cuts at: 355 1067 1381 1741 2754 2999 355
Size: 712 314 360 1013 245 1312
Fragments arranged by size:
1312 1013 712 360 314 245
NaeI GCC'GGC
Cuts at: 2137 2137
Size: 3956
NarI GG'CG_CC
Cuts at: 1387 1635 1387
Size: 248 3708
NcoI C'CATG_G
Cuts at: 107 145 107
Size: 38 3918
NdeI CA'TA_TG
Cuts at: 614 614
Size: 3956
NgoMIV G'CCGG_C
Cuts at: 2135 2135
Size: 3956
NotI GC'GGCC_GC
Cuts at: 267 267
Size: 3956
NspI r_CATG'y
Cuts at: 1009 1509 2416 1009
Size: 500 907 2549
Fragments arranged by size:
2549 907 500
PciI A'CATG_T
Cuts at: 2412 2412
Size: 3956
PflMI CCAn_nnn'nTGG
Cuts at: 186 186
Size: 3956
PfoI T'CCnGG_A
Cuts at: 506 850 506
Size: 344 3612
PmeI GTTT'AAAC
Cuts at: 955 955
Size: 3956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3924
PsiI TTA'TAA
Cuts at: 814 814
Size: 3956
PstI C_TGCA'G
Cuts at: 1003 1688 1003
Size: 685 3271
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3956
PvuII CAG'CTG
Cuts at: 355 1381 1741 355
Size: 1026 360 2570
Fragments arranged by size:
2570 1026 360
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 3956
SacI G_AGCT'C
Cuts at: 161 970 161
Size: 809 3147
SalI G'TCGA_C
Cuts at: 993 993
Size: 3956
SapI GCTCTTCn'nnn_
Cuts at: 1979 2189 1979
Size: 210 3746
SbfI CC_TGCA'GG
Cuts at: 1003 1003
Size: 3956
ScaI AGT'ACT
Cuts at: 437 3473 437
Size: 3036 920
SfcI C'TryA_G
Cuts at: 33 999 1684 2677 2868 33
Size: 966 685 993 191 1121
Fragments arranged by size:
1121 993 966 685 191
SfoI GGC'GCC
Cuts at: 1388 1636 1388
Size: 248 3708
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
SmaI CCC'GGG
Cuts at: 978 978
Size: 3956
SmlI C'TyrA_G
Cuts at: 1111 2026 2518 2780 1111
Size: 915 492 262 2287
Fragments arranged by size:
2287 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3956
SphI G_CATG'C
Cuts at: 1009 1509 1009
Size: 500 3456
StuI AGG'CCT
Cuts at: 184 184
Size: 3956
StyI C'CwwG_G
Cuts at: 107 145 185 1090 1444 107
Size: 38 40 905 354 2619
Fragments arranged by size:
2619 905 354 40 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 1539 1672 2131 2340 262
Size: 1277 133 459 209 1878
Fragments arranged by size:
1878 1277 459 209 133
TatI w'GTAC_w
Cuts at: 435 570 3471 435
Size: 135 2901 920
Fragments arranged by size:
2901 920 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 3924
Tth111I GACn'n_nGTC
Cuts at: 1753 1753
Size: 3956
XbaI T'CTAG_A
Cuts at: 987 987
Size: 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 504 1451 504
Size: 947 3009
XmaI C'CCGG_G
Cuts at: 976 976
Size: 3956
XmnI GAAnn'nnTTC
Cuts at: 212 212
Size: 3956
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NspI PciI PflMI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NheI NruI NsiI PacI
PasI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI