Restriction map for
pF1KB6310
(Circular) (Six-base) MAPSORT of: pF1KB6310.seq Check: 525 from: 1 to: 3953
pF1KB6310 3953 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 991 991
Size: 3953
Acc65I G'GTAC_C
Cuts at: 969 969
Size: 3953
AclI AA'CG_TT
Cuts at: 947 3793 947
Size: 2846 1107
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 715 1778 2210 2957 3225 673
Size: 42 1063 432 747 268 1401
Fragments arranged by size:
1401 1063 747 432 268 42
AfeI AGC'GCT
Cuts at: 600 600
Size: 3953
AflIII A'CryG_T
Cuts at: 1305 2409 3386 1305
Size: 1104 977 1872
Fragments arranged by size:
1872 1104 977
AgeI A'CCGG_T
Cuts at: 2341 2341
Size: 3953
AhdI GACnn_n'nnGTC
Cuts at: 321 321
Size: 3953
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 922 954 922
Size: 32 3921
AlwNI CAG_nnn'CTG
Cuts at: 1258 2825 1258
Size: 1567 2386
ApaI G_GGCC'C
Cuts at: 858 858
Size: 3953
ApaLI G'TGCA_C
Cuts at: 2723 2723
Size: 3953
ApoI r'AATT_y
Cuts at: 957 3306 3562 957
Size: 2349 256 1348
Fragments arranged by size:
2349 1348 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 201 425 634 850 865 885 973 201
Size: 224 209 216 15 20 88 3181
Fragments arranged by size:
3181 224 216 209 88 20 15
BamHI G'GATC_C
Cuts at: 270 978 270
Size: 708 3245
BanI G'GyrC_C
Cuts at: 969 1383 1631 1666 969
Size: 414 248 35 3256
Fragments arranged by size:
3256 414 248 35
BanII G_rGCy'C
Cuts at: 115 788 858 892 967 1997 115
Size: 673 70 34 75 1030 2071
Fragments arranged by size:
2071 1030 673 75 70 34
BbeI G_GCGC'C
Cuts at: 1387 1635 1387
Size: 248 3705
BbvCI CC'TCA_GC
Cuts at: 500 500
Size: 3953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 556 590 556
Size: 34 3919
BciVI GTATCCnnnnn_n'
Cuts at: 1851 2612 3953 1851
Size: 761 1341 1851
Fragments arranged by size:
1851 1341 761
BclI T'GATC_A
Cuts at: 1473 1473
Size: 3953
BglI GCCn_nnn'nGGC
Cuts at: 475 650 475
Size: 175 3778
BglII A'GATC_T
Cuts at: 192 669 1468 3505 192
Size: 477 799 2037 640
Fragments arranged by size:
2037 799 640 477
BlpI GC'TnA_GC
Cuts at: 1065 1065
Size: 3953
Bme1580I G_kGCm'C
Cuts at: 147 471 858 1578 1671 2727 147
Size: 324 387 720 93 1056 1373
Fragments arranged by size:
1373 1056 720 387 324 93
BmrI ACTGGGnnnn_n'
Cuts at: 698 1165 1246 1581 3725 698
Size: 467 81 335 2144 926
Fragments arranged by size:
2144 926 467 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 3953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 303 483 694 209
Size: 94 180 211 3468
Fragments arranged by size:
3468 211 180 94
Bpu10I CC'TnA_GC
Cuts at: 500 500
Size: 3953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2008 2500 2798 1129
Size: 879 492 298 2284
Fragments arranged by size:
2284 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 122 3657 122
Size: 3535 418
BsaAI yAC'GTr
Cuts at: 1306 1306
Size: 3953
BsaBI GATnn'nnATC
Cuts at: 6 1467 3504 6
Size: 1461 2037 455
Fragments arranged by size:
2037 1461 455
BsaHI Gr'CG_yC
Cuts at: 1384 1632 2334 3830 1384
Size: 248 702 1496 1507
Fragments arranged by size:
1507 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1142 1663 2341 2615 2762 3173 1142
Size: 521 678 274 147 411 1922
Fragments arranged by size:
1922 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 207 237 898 928 207
Size: 30 661 30 3232
Fragments arranged by size:
3232 661 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 637 732 796 940 637
Size: 95 64 144 3650
Fragments arranged by size:
3650 144 95 64
BseYI C'CCAG_C
Cuts at: 470 482 495 748 790 817 1378 2136 2713
Size: 12 13 253 42 27 561 758 577
Cuts at: 2713 3209 470
Size: 496 1214
Fragments arranged by size:
1214 758 577 561 496 253 42 27
13 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 613 403
Size: 210 3743
BsiEI CG_ry'CG
Cuts at: 104 1495 1541 2749 104
Size: 1391 46 1208 1308
Fragments arranged by size:
1391 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 967 1745 1935 2727 967
Size: 778 190 792 2193
Fragments arranged by size:
2193 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 115 147 471 788 858 892 967 1578 1671
Size: 32 324 317 70 34 75 611 93
Cuts at: 1671 1745 1935 1997 2727 115
Size: 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 324 317 190 93 75
74 70 62 34 32
BspEI T'CCGG_A
Cuts at: 3173 3173
Size: 3953
BspHI T'CATG_A
Cuts at: 3129 3129
Size: 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1003 1217 1519 1900 1003
Size: 214 302 381 3056
Fragments arranged by size:
3056 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2245 2299 3783 3948 50
Size: 2195 54 1484 165 55
Fragments arranged by size:
2195 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1865 1865
Size: 3953
BsrFI r'CCGG_y
Cuts at: 1951 2132 2341 1951
Size: 181 209 3563
Fragments arranged by size:
3563 209 181
BssSI C'ACGA_G
Cuts at: 2224 2582 2224
Size: 358 3595
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 1330
Size: 3953
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstXI CCAn_nnnn'nTGG
Cuts at: 420 420
Size: 3953
BstYI r'GATC_y
Cuts at: 1 192 270 669 978 1445 1468 1803 2049
Size: 191 78 399 309 467 23 335 246
Cuts at: 2049 3061 3147 3159 3505 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 399 346 335 309 246
191 86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 779 779
Size: 3953
BtgI C'CryG_G
Cuts at: 107 218 413 419 902 107
Size: 111 195 6 483 3158
Fragments arranged by size:
3158 483 195 111 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 1196 1196
Size: 3953
DraI TTT'AAA
Cuts at: 952 952
Size: 3953
DrdI GACnn_nn'nnGTC
Cuts at: 1659 2517 1659
Size: 858 3095
EaeI y'GGCC_r
Cuts at: 356 416 1538 1712 2103 2130 3475 356
Size: 60 1122 174 391 27 1345 834
Fragments arranged by size:
1345 1122 834 391 174 60 27
EagI C'GGCC_G
Cuts at: 1538 1538
Size: 3953
EarI CTCTTCn'nnn_
Cuts at: 614 1976 2186 614
Size: 1362 210 2381
Fragments arranged by size:
2381 1362 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2161 2471 2617 3763 2161
Size: 310 146 1146 2351
Fragments arranged by size:
2351 1146 310 146
EcoICRI GAG'CTC
Cuts at: 965 965
Size: 3953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 303 483 673 694 715 1778 2210 2957
Size: 94 180 190 21 21 1063 432 747
Cuts at: 2957 3225 209
Size: 268 937
Fragments arranged by size:
1063 937 747 432 268 190 180 94
21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 922 922
Size: 3953
EcoO109I rG'GnC_Cy
Cuts at: 323 854 862 875 1092 323
Size: 531 8 13 217 3184
Fragments arranged by size:
3184 531 217 13 8
EcoRI G'AATT_C
Cuts at: 957 957
Size: 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3953
FspI TGC'GCA
Cuts at: 1734 1734
Size: 3953
HaeII r_GCGC'y
Cuts at: 602 1387 1635 2657 3636 602
Size: 785 248 1022 979 919
Fragments arranged by size:
1022 979 919 785 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 307 339 898 930 1795 1825 1827
Size: 32 91 32 559 32 865 30 2
Cuts at: 1827 1857 184
Size: 30 2280
Fragments arranged by size:
2280 865 559 91 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 992 992
Size: 3953
KasI G'GCGC_C
Cuts at: 1383 1631 1383
Size: 248 3705
KpnI G_GTAC'C
Cuts at: 973 973
Size: 3953
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 338 2624 2808 338
Size: 2286 184 1483
Fragments arranged by size:
2286 1483 184
MscI TGG'CCA
Cuts at: 358 1714 358
Size: 1356 2597
MslI CAynn'nnrTG
Cuts at: 418 1942 2069 418
Size: 1524 127 2302
Fragments arranged by size:
2302 1524 127
MspA1I CmG'CkG
Cuts at: 474 499 767 799 911 1064 1378 1738 2751
Size: 25 268 32 112 153 314 360 1013
Cuts at: 2751 2996 474
Size: 245 1431
Fragments arranged by size:
1431 1013 360 314 268 245 153 112
32 25
NaeI GCC'GGC
Cuts at: 2134 2134
Size: 3953
NarI GG'CG_CC
Cuts at: 1384 1632 1384
Size: 248 3705
NcoI C'CATG_G
Cuts at: 107 419 902 107
Size: 312 483 3158
Fragments arranged by size:
3158 483 312
NgoMIV G'CCGG_C
Cuts at: 2132 2132
Size: 3953
NruI TCG'CGA
Cuts at: 126 126
Size: 3953
NspI r_CATG'y
Cuts at: 1006 1506 2413 1006
Size: 500 907 2546
Fragments arranged by size:
2546 907 500
PasI CC'CwG_GG
Cuts at: 168 648 168
Size: 480 3473
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PflMI CCAn_nnn'nTGG
Cuts at: 672 672
Size: 3953
PfoI T'CCnGG_A
Cuts at: 524 524
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3921
PpuMI rG'GwC_Cy
Cuts at: 323 862 875 323
Size: 539 13 3401
Fragments arranged by size:
3401 539 13
PshAI GACnn'nnGTC
Cuts at: 767 767
Size: 3953
PspOMI G'GGCC_C
Cuts at: 854 854
Size: 3953
PstI C_TGCA'G
Cuts at: 558 833 1000 1685 558
Size: 275 167 685 2826
Fragments arranged by size:
2826 685 275 167
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 499 767 1378 1738 499
Size: 268 611 360 2714
Fragments arranged by size:
2714 611 360 268
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 967 967
Size: 3953
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SanDI GG'GwC_CC
Cuts at: 875 875
Size: 3953
SapI GCTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 3470 3470
Size: 3953
SfcI C'TryA_G
Cuts at: 33 554 829 996 1681 2674 2865 33
Size: 521 275 167 685 993 191 1121
Fragments arranged by size:
1121 993 685 521 275 191 167
SfoI GGC'GCC
Cuts at: 1385 1633 1385
Size: 248 3705
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 852 887 975 852
Size: 35 88 3830
Fragments arranged by size:
3830 88 35
SmlI C'TyrA_G
Cuts at: 1108 2023 2515 2777 1108
Size: 915 492 262 2284
Fragments arranged by size:
2284 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
StuI AGG'CCT
Cuts at: 736 736
Size: 3953
StyI C'CwwG_G
Cuts at: 107 274 419 858 902 1087 1441 107
Size: 167 145 439 44 185 354 2619
Fragments arranged by size:
2619 439 354 185 167 145 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1536 1669 2128 2337 1536
Size: 133 459 209 3152
Fragments arranged by size:
3152 459 209 133
TatI w'GTAC_w
Cuts at: 3468 3468
Size: 3953
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 207 239 3470 3502 207
Size: 32 3231 32 658
Fragments arranged by size:
3231 658 32 32
Tth111I GACn'n_nGTC
Cuts at: 232 1750 232
Size: 1518 2435
XbaI T'CTAG_A
Cuts at: 984 984
Size: 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1448 1448
Size: 3953
XmaI C'CCGG_G
Cuts at: 850 885 973 850
Size: 35 88 3830
Fragments arranged by size:
3830 88 35
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PmlI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI