Restriction map for
pF1KB6305
(Circular) (Six-base) MAPSORT of: pF1KB6305.seq Check: 127 from: 1 to: 3887
pF1KB6305 3887 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 881 3727 881
Size: 2846 1041
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1712 2144 2891 3159 1712
Size: 432 747 268 2440
Fragments arranged by size:
2440 747 432 268
AflIII A'CryG_T
Cuts at: 1239 2343 3320 1239
Size: 1104 977 1806
Fragments arranged by size:
1806 1104 977
AgeI A'CCGG_T
Cuts at: 221 2275 221
Size: 2054 1833
AleI CACnn'nnGTG
Cuts at: 248 676 248
Size: 428 3459
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 683 715 683
Size: 32 3855
AlwNI CAG_nnn'CTG
Cuts at: 539 594 1192 2759 539
Size: 55 598 1567 1667
Fragments arranged by size:
1667 1567 598 55
ApaI G_GGCC'C
Cuts at: 733 733
Size: 3887
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 189 891 3240 3496 189
Size: 702 2349 256 580
Fragments arranged by size:
2349 702 580 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 907 907
Size: 3887
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 903 1317 1565 1600 903
Size: 414 248 35 3190
Fragments arranged by size:
3190 414 248 35
BanII G_rGCy'C
Cuts at: 658 733 901 1931 658
Size: 75 168 1030 2614
Fragments arranged by size:
2614 1030 168 75
BbeI G_GCGC'C
Cuts at: 1321 1569 1321
Size: 248 3639
BbsI GAAGACnn'nnnn_
Cuts at: 319 819 319
Size: 500 3387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3853
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglI GCCn_nnn'nGGC
Cuts at: 523 523
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 999 999
Size: 3887
Bme1580I G_kGCm'C
Cuts at: 733 1512 1605 2661 733
Size: 779 93 1056 1959
Fragments arranged by size:
1959 1056 779 93
BmrI ACTGGGnnnn_n'
Cuts at: 285 660 1099 1180 1515 3659 285
Size: 375 439 81 335 2144 513
Fragments arranged by size:
2144 513 439 375 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 248 353 621 795 199
Size: 49 105 268 174 3291
Fragments arranged by size:
3291 268 174 105 49
Bpu10I CC'TnA_GC
Cuts at: 346 346
Size: 3887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 329 843 3591 329
Size: 514 2748 625
Fragments arranged by size:
2748 625 514
BsaAI yAC'GTr
Cuts at: 375 1240 375
Size: 865 3022
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 1318 1566 2268 3764 1318
Size: 248 702 1496 1441
Fragments arranged by size:
1496 1441 702 248
BsaWI w'CCGG_w
Cuts at: 144 221 1076 1597 2275 2549 2696 3107 144
Size: 77 855 521 678 274 147 411 924
Fragments arranged by size:
924 855 678 521 411 274 147 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 640 670 683 713 640
Size: 30 13 30 3814
Fragments arranged by size:
3814 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 633 633
Size: 3887
BseYI C'CCAG_C
Cuts at: 492 554 1312 2070 2647 3143 492
Size: 62 758 758 577 496 1236
Fragments arranged by size:
1236 758 758 577 496 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 3887
BsiEI CG_ry'CG
Cuts at: 104 1429 1475 2683 104
Size: 1325 46 1208 1308
Fragments arranged by size:
1325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 901 1679 1869 2661 901
Size: 778 190 792 2127
Fragments arranged by size:
2127 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 658 733 901 1512 1605 1679 1869 1931 2661
Size: 75 168 611 93 74 190 62 730
Cuts at: 2661 658
Size: 1884
Fragments arranged by size:
1884 730 611 190 168 93 75 74
62
BspEI T'CCGG_A
Cuts at: 144 3107 144
Size: 2963 924
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 617 937 1151 1453 1834 617
Size: 320 214 302 381 2670
Fragments arranged by size:
2670 381 320 302 214
BsrBI CCG'CTC
Cuts at: 50 2179 2233 3717 3882 50
Size: 2129 54 1484 165 55
Fragments arranged by size:
2129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 738 1799 738
Size: 1061 2826
BsrFI r'CCGG_y
Cuts at: 221 430 1885 2066 2275 221
Size: 209 1455 181 209 1833
Fragments arranged by size:
1833 1455 209 209 181
BssSI C'ACGA_G
Cuts at: 2158 2516 2158
Size: 358 3529
BstAPI GCAn_nnn'nTGC
Cuts at: 1264 1264
Size: 3887
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstXI CCAn_nnnn'nTGG
Cuts at: 358 358
Size: 3887
BstYI r'GATC_y
Cuts at: 1 912 1379 1402 1737 1983 2995 3081 3093
Size: 911 467 23 335 246 1012 86 12
Cuts at: 3093 3439 1
Size: 346 449
Fragments arranged by size:
1012 911 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 674 768 674
Size: 94 3793
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 426 855 1837 2018 2181 426
Size: 429 982 181 163 2132
Fragments arranged by size:
2132 982 429 181 163
BtsI GCAGTG_nn'
Cuts at: 294 1130 294
Size: 836 3051
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DraIII CAC_nnn'GTG
Cuts at: 300 300
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 1472 1646 2037 2064 3409 1472
Size: 174 391 27 1345 1950
Fragments arranged by size:
1950 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 899 899
Size: 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 248 353 621 795 1712 2144 2891 3159
Size: 49 105 268 174 917 432 747 268
Cuts at: 3159 199
Size: 927
Fragments arranged by size:
927 917 747 432 268 268 174 105
49
EcoNI CCTnn'n_nnAGG
Cuts at: 598 598
Size: 3887
EcoO109I rG'GnC_Cy
Cuts at: 138 257 729 730 1026 138
Size: 119 472 1 296 2999
Fragments arranged by size:
2999 472 296 119 1
EcoRI G'AATT_C
Cuts at: 189 891 189
Size: 702 3185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3887
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 1321 1569 2591 3570 1321
Size: 248 1022 979 1638
Fragments arranged by size:
1638 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 276 308 1729 1759 1761 1791 276
Size: 32 1421 30 2 30 2372
Fragments arranged by size:
2372 1421 32 30 30 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
KasI G'GCGC_C
Cuts at: 1317 1565 1317
Size: 248 3639
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 147 902 2558 2742 147
Size: 755 1656 184 1292
Fragments arranged by size:
1656 1292 755 184
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 248 676 1876 2003 248
Size: 428 1200 127 2132
Fragments arranged by size:
2132 1200 428 127
MspA1I CmG'CkG
Cuts at: 289 492 582 998 1312 1672 2685 2930 289
Size: 203 90 416 314 360 1013 245 1246
Fragments arranged by size:
1246 1013 416 360 314 245 203 90
NaeI GCC'GGC
Cuts at: 432 2068 432
Size: 1636 2251
NarI GG'CG_CC
Cuts at: 1318 1566 1318
Size: 248 3639
NcoI C'CATG_G
Cuts at: 674 768 674
Size: 94 3793
NgoMIV G'CCGG_C
Cuts at: 430 2066 430
Size: 1636 2251
NspI r_CATG'y
Cuts at: 940 1440 2347 940
Size: 500 907 2480
Fragments arranged by size:
2480 907 500
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PflMI CCAn_nnn'nTGG
Cuts at: 744 744
Size: 3887
PfoI T'CCnGG_A
Cuts at: 811 853 811
Size: 42 3845
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 683 715 3026 3058 683
Size: 32 2311 32 1512
Fragments arranged by size:
2311 1512 32 32
PpuMI rG'GwC_Cy
Cuts at: 138 257 138
Size: 119 3768
PspOMI G'GGCC_C
Cuts at: 729 729
Size: 3887
PstI C_TGCA'G
Cuts at: 599 934 1619 599
Size: 335 685 2867
Fragments arranged by size:
2867 685 335
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 289 492 582 1312 1672 289
Size: 203 90 730 360 2504
Fragments arranged by size:
2504 730 360 203 90
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 901 901
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SanDI GG'GwC_CC
Cuts at: 257 257
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SfcI C'TryA_G
Cuts at: 33 595 821 930 1615 2608 2799 33
Size: 562 226 109 685 993 191 1121
Fragments arranged by size:
1121 993 685 562 226 191 109
SfoI GGC'GCC
Cuts at: 1319 1567 1319
Size: 248 3639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 909 909
Size: 3887
SmlI C'TyrA_G
Cuts at: 1042 1957 2449 2711 1042
Size: 915 492 262 2218
Fragments arranged by size:
2218 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StyI C'CwwG_G
Cuts at: 674 768 1021 1375 674
Size: 94 253 354 3186
Fragments arranged by size:
3186 354 253 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 148 267 484 847 1470 1603 2062 2271 148
Size: 119 217 363 623 133 459 209 1764
Fragments arranged by size:
1764 623 459 363 217 209 133 119
TatI w'GTAC_w
Cuts at: 3402 3402
Size: 3887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 3855
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 223 579 721 1382 223
Size: 356 142 661 2728
Fragments arranged by size:
2728 661 356 142
XmaI C'CCGG_G
Cuts at: 907 907
Size: 3887
XmnI GAAnn'nnTTC
Cuts at: 316 316
Size: 3887
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI