Restriction map for
pF1KB6292
(Circular) (Six-base) MAPSORT of: pF1KB6292.seq Check: 2168 from: 1 to: 5480
pF1KB6292 5480 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1859 1955 1859
Size: 96 5384
AccI GT'mk_AC
Cuts at: 1337 2518 1337
Size: 1181 4299
Acc65I G'GTAC_C
Cuts at: 2430 2496 2430
Size: 66 5414
AclI AA'CG_TT
Cuts at: 1733 5320 1733
Size: 3587 1893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1717 1789 3305 3737 4484 4752 1717
Size: 72 1516 432 747 268 2445
Fragments arranged by size:
2445 1516 747 432 268 72
AfeI AGC'GCT
Cuts at: 1278 1278
Size: 5480
AflIII A'CryG_T
Cuts at: 331 1133 1578 2832 3936 4913 331
Size: 802 445 1254 1104 977 898
Fragments arranged by size:
1254 1104 977 898 802 445
AgeI A'CCGG_T
Cuts at: 3868 3868
Size: 5480
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 490 490
Size: 5480
AlwNI CAG_nnn'CTG
Cuts at: 154 2785 4352 154
Size: 2631 1567 1282
Fragments arranged by size:
2631 1567 1282
ApaI G_GGCC'C
Cuts at: 909 1093 909
Size: 184 5296
ApaLI G'TGCA_C
Cuts at: 1786 2014 4250 1786
Size: 228 2236 3016
Fragments arranged by size:
3016 2236 228
ApoI r'AATT_y
Cuts at: 2273 2484 4833 5089 2273
Size: 211 2349 256 2664
Fragments arranged by size:
2664 2349 256 211
AseI AT'TA_AT
Cuts at: 20 20
Size: 5480
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5480
AvaI C'yCGr_G
Cuts at: 503 884 1320 1398 2045 2224 2500 503
Size: 381 436 78 647 179 276 3483
Fragments arranged by size:
3483 647 436 381 276 179 78
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 212 245 212
Size: 33 5447
BamHI G'GATC_C
Cuts at: 2469 2505 2469
Size: 36 5444
BanI G'GyrC_C
Cuts at: 1470 2142 2430 2496 2910 3158 3193 1470
Size: 672 288 66 414 248 35 3757
Fragments arranged by size:
3757 672 414 288 248 66 35
BanII G_rGCy'C
Cuts at: 865 909 1048 1093 1162 1405 1425 2494 3524
Size: 44 139 45 69 243 20 1069 1030
Cuts at: 3524 865
Size: 2821
Fragments arranged by size:
2821 1069 1030 243 139 69 45 44
20
BbeI G_GCGC'C
Cuts at: 1474 2146 2914 3162 1474
Size: 672 768 248 3792
Fragments arranged by size:
3792 768 672 248
BbsI GAAGACnn'nnnn_
Cuts at: 858 926 858
Size: 68 5412
BbvCI CC'TCA_GC
Cuts at: 1602 1602
Size: 5480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1332 1366 1389 1423 1332
Size: 34 23 34 5389
Fragments arranged by size:
5389 34 34 23
BciVI GTATCCnnnnn_n'
Cuts at: 3378 4139 5480 3378
Size: 761 1341 3378
Fragments arranged by size:
3378 1341 761
BclI T'GATC_A
Cuts at: 3000 3000
Size: 5480
BglI GCCn_nnn'nGGC
Cuts at: 1570 1570
Size: 5480
BglII A'GATC_T
Cuts at: 2163 2385 2995 5032 2163
Size: 222 610 2037 2611
Fragments arranged by size:
2611 2037 610 222
BlpI GC'TnA_GC
Cuts at: 2592 2592
Size: 5480
Bme1580I G_kGCm'C
Cuts at: 331 909 1093 1333 1790 1993 2018 3105 3198
Size: 578 184 240 457 203 25 1087 93
Cuts at: 3198 4254 331
Size: 1056 1557
Fragments arranged by size:
1557 1087 1056 578 457 240 203 184
93 25
BmgBI CAC'GTC
Cuts at: 856 2232 856
Size: 1376 4104
BmrI ACTGGGnnnn_n'
Cuts at: 2001 2064 2692 2773 3108 5252 2001
Size: 63 628 81 335 2144 2229
Fragments arranged by size:
2229 2144 628 335 81 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3847 3847
Size: 5480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1032 1420 1982 1032
Size: 388 562 4530
Fragments arranged by size:
4530 562 388
Bpu10I CC'TnA_GC
Cuts at: 1273 1602 1273
Size: 329 5151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1085 1921 2089 2656 3535 4027 4325 1024
Size: 61 836 168 567 879 492 298 2179
Fragments arranged by size:
2179 879 836 567 492 298 168 61
BsaI GGTCTCn'nnnn_
Cuts at: 951 1550 5184 951
Size: 599 3634 1247
Fragments arranged by size:
3634 1247 599
BsaAI yAC'GTr
Cuts at: 965 2833 965
Size: 1868 3612
BsaBI GATnn'nnATC
Cuts at: 6 541 2994 5031 6
Size: 535 2453 2037 455
Fragments arranged by size:
2453 2037 535 455
BsaHI Gr'CG_yC
Cuts at: 1471 2143 2911 3159 3861 5357 1471
Size: 672 768 248 702 1496 1594
Fragments arranged by size:
1594 1496 768 702 672 248
BsaWI w'CCGG_w
Cuts at: 2669 3190 3868 4142 4289 4700 2669
Size: 521 678 274 147 411 3449
Fragments arranged by size:
3449 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1099 1129 2050 2080 1099
Size: 30 921 30 4499
Fragments arranged by size:
4499 921 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1339 2341 2346 1339
Size: 1002 5 4473
Fragments arranged by size:
4473 1002 5
BseYI C'CCAG_C
Cuts at: 1621 2410 2905 3663 4240 4736 1621
Size: 789 495 758 577 496 2365
Fragments arranged by size:
2365 789 758 577 496 495
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 2011 2023 2385 1606
Size: 405 12 362 4701
Fragments arranged by size:
4701 405 362 12
BsiEI CG_ry'CG
Cuts at: 104 3022 3068 4276 104
Size: 2918 46 1208 1308
Fragments arranged by size:
2918 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 697 1048 1109 1405 1790 2018 2068 2494 3272
Size: 351 61 296 385 228 50 426 778
Cuts at: 3272 3462 4254 697
Size: 190 792 1923
Fragments arranged by size:
1923 792 778 426 385 351 296 228
190 61 50
BsmI GAATG_Cn'
Cuts at: 1732 1900 1732
Size: 168 5312
Bsp1286I G_dGCh'C
Cuts at: 331 697 865 909 1048 1093 1109 1162 1333
Size: 366 168 44 139 45 16 53 171
Cuts at: 1333 1405 1425 1790 1993 2018 2068 2494 3105
Size: 72 20 365 203 25 50 426 611
Cuts at: 3105 3198 3272 3462 3524 4254 331
Size: 93 74 190 62 730 1557
Fragments arranged by size:
1557 730 611 426 366 365 203 190
171 168 139 93 74 72 62 53
50 45 44 25 20 16
BspEI T'CCGG_A
Cuts at: 4700 4700
Size: 5480
BspHI T'CATG_A
Cuts at: 2396 4656 2396
Size: 2260 3220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1859 1955 2530 2744 3046 3427 1859
Size: 96 575 214 302 381 3912
Fragments arranged by size:
3912 575 381 302 214 96
BsrBI CCG'CTC
Cuts at: 50 2052 3772 3826 5310 5475 50
Size: 2002 1720 54 1484 165 55
Fragments arranged by size:
2002 1720 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3392 3392
Size: 5480
BsrFI r'CCGG_y
Cuts at: 1669 2219 3478 3659 3868 1669
Size: 550 1259 181 209 3281
Fragments arranged by size:
3281 1259 550 209 181
BsrGI T'GTAC_A
Cuts at: 172 172
Size: 5480
BssSI C'ACGA_G
Cuts at: 3751 4109 3751
Size: 358 5122
BstAPI GCAn_nnn'nTGC
Cuts at: 1736 1988 2857 1736
Size: 252 869 4359
Fragments arranged by size:
4359 869 252
BstBI TT'CG_AA
Cuts at: 3841 3841
Size: 5480
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5480
BstYI r'GATC_y
Cuts at: 1 410 2163 2385 2469 2505 2972 2995 3330
Size: 409 1753 222 84 36 467 23 335
Cuts at: 3330 3576 4588 4674 4686 5032 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1753 1012 467 449 409 346 335 246
222 86 84 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 2358 2358
Size: 5480
BtgI C'CryG_G
Cuts at: 1550 1568 2282 1550
Size: 18 714 4748
Fragments arranged by size:
4748 714 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1624 3430 3611 3774 1624
Size: 1806 181 163 3330
Fragments arranged by size:
3330 1806 181 163
BtsI GCAGTG_nn'
Cuts at: 2723 2723
Size: 5480
DraI TTT'AAA
Cuts at: 2479 2479
Size: 5480
DrdI GACnn_nn'nnGTC
Cuts at: 3186 4044 3186
Size: 858 4622
EaeI y'GGCC_r
Cuts at: 1752 2279 3065 3239 3630 3657 5002 1752
Size: 527 786 174 391 27 1345 2230
Fragments arranged by size:
2230 1345 786 527 391 174 27
EagI C'GGCC_G
Cuts at: 3065 3065
Size: 5480
EarI CTCTTCn'nnn_
Cuts at: 158 281 452 1321 3503 3713 158
Size: 123 171 869 2182 210 1925
Fragments arranged by size:
2182 1925 869 210 171 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 250 3688 3998 4144 5290 250
Size: 3438 310 146 1146 440
Fragments arranged by size:
3438 1146 440 310 146
EcoICRI GAG'CTC
Cuts at: 1046 1403 2492 1046
Size: 357 1089 4034
Fragments arranged by size:
4034 1089 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1032 1420 1717 1789 1982 3305 3737 4484 4752
Size: 388 297 72 193 1323 432 747 268
Cuts at: 4752 1032
Size: 1760
Fragments arranged by size:
1760 1323 747 432 388 297 268 193
72
EcoNI CCTnn'n_nnAGG
Cuts at: 249 249
Size: 5480
EcoO109I rG'GnC_Cy
Cuts at: 792 834 906 1090 2619 792
Size: 42 72 184 1529 3653
Fragments arranged by size:
3653 1529 184 72 42
EcoRI G'AATT_C
Cuts at: 2484 2484
Size: 5480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1257 3499 1257
Size: 2242 3238
FspI TGC'GCA
Cuts at: 3261 3261
Size: 5480
HaeII r_GCGC'y
Cuts at: 1280 1474 2146 2914 3162 4184 5163 1280
Size: 194 672 768 248 1022 979 1597
Fragments arranged by size:
1597 1022 979 768 672 248 194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3322 3352 3354 3384 3322
Size: 30 2 30 5418
Fragments arranged by size:
5418 30 30 2
HincII GTy'rAC
Cuts at: 2519 2519
Size: 5480
HindIII A'AGCT_T
Cuts at: 932 932
Size: 5480
KasI G'GCGC_C
Cuts at: 1470 2142 2910 3158 1470
Size: 672 768 248 3792
Fragments arranged by size:
3792 768 672 248
KpnI G_GTAC'C
Cuts at: 2434 2500 2434
Size: 66 5414
MluI A'CGCG_T
Cuts at: 1133 4913 1133
Size: 3780 1700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 797 4151 4335 797
Size: 3354 184 1942
Fragments arranged by size:
3354 1942 184
MscI TGG'CCA
Cuts at: 3241 3241
Size: 5480
MslI CAynn'nnrTG
Cuts at: 112 1893 1977 2248 3469 3596 112
Size: 1781 84 271 1221 127 1996
Fragments arranged by size:
1996 1781 1221 271 127 84
MspA1I CmG'CkG
Cuts at: 1695 2591 2905 3265 4278 4523 1695
Size: 896 314 360 1013 245 2652
Fragments arranged by size:
2652 1013 896 360 314 245
NaeI GCC'GGC
Cuts at: 3661 3661
Size: 5480
NarI GG'CG_CC
Cuts at: 1471 2143 2911 3159 1471
Size: 672 768 248 3792
Fragments arranged by size:
3792 768 672 248
NgoMIV G'CCGG_C
Cuts at: 3659 3659
Size: 5480
NspI r_CATG'y
Cuts at: 1582 2533 3033 3940 1582
Size: 951 500 907 3122
Fragments arranged by size:
3122 951 907 500
PasI CC'CwG_GG
Cuts at: 197 1066 1451 197
Size: 869 385 4226
Fragments arranged by size:
4226 869 385
PciI A'CATG_T
Cuts at: 1578 3936 1578
Size: 2358 3122
PfoI T'CCnGG_A
Cuts at: 636 636
Size: 5480
PmeI GTTT'AAAC
Cuts at: 2479 2479
Size: 5480
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4619 4651 4619
Size: 32 5448
PpuMI rG'GwC_Cy
Cuts at: 792 834 792
Size: 42 5438
PspOMI G'GGCC_C
Cuts at: 905 1089 905
Size: 184 5296
PspXI vC'TCGA_Gb
Cuts at: 2224 2224
Size: 5480
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1123 1155 1123
Size: 32 5448
PstI C_TGCA'G
Cuts at: 1856 2527 3212 1856
Size: 671 685 4124
Fragments arranged by size:
4124 685 671
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5480
PvuII CAG'CTG
Cuts at: 2905 3265 2905
Size: 360 5120
RsrII CG'GwC_CG
Cuts at: 3675 3675
Size: 5480
SacI G_AGCT'C
Cuts at: 1048 1405 2494 1048
Size: 357 1089 4034
Fragments arranged by size:
4034 1089 357
SalI G'TCGA_C
Cuts at: 2517 2517
Size: 5480
SapI GCTCTTCn'nnn_
Cuts at: 3503 3713 3503
Size: 210 5270
SbfI CC_TGCA'GG
Cuts at: 2527 2527
Size: 5480
ScaI AGT'ACT
Cuts at: 4997 4997
Size: 5480
SexAI A'CCwGG_T
Cuts at: 1865 1865
Size: 5480
SfcI C'TryA_G
Cuts at: 33 1338 1527 1852 2097 2258 2523 3208 4201
Size: 1305 189 325 245 161 265 685 993
Cuts at: 4201 4392 33
Size: 191 1121
Fragments arranged by size:
1305 1121 993 685 325 265 245 191
189 161
SfoI GGC'GCC
Cuts at: 1472 2144 2912 3160 1472
Size: 672 768 248 3792
Fragments arranged by size:
3792 768 672 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5480
SmaI CCC'GGG
Cuts at: 505 2047 2502 505
Size: 1542 455 3483
Fragments arranged by size:
3483 1542 455
SmlI C'TyrA_G
Cuts at: 1039 1100 1936 2104 2224 2635 3550 4042 4304
Size: 61 836 168 120 411 915 492 262
Cuts at: 4304 1039
Size: 2215
Fragments arranged by size:
2215 915 836 492 411 262 168 120
61
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5480
SphI G_CATG'C
Cuts at: 2533 3033 2533
Size: 500 4980
SspI AAT'ATT
Cuts at: 400 400
Size: 5480
StuI AGG'CCT
Cuts at: 1525 1525
Size: 5480
StyI C'CwwG_G
Cuts at: 250 2156 2614 2968 250
Size: 1906 458 354 2762
Fragments arranged by size:
2762 1906 458 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2020 3063 3196 3655 3864 2020
Size: 1043 133 459 209 3636
Fragments arranged by size:
3636 1043 459 209 133
TatI w'GTAC_w
Cuts at: 172 740 4995 172
Size: 568 4255 657
Fragments arranged by size:
4255 657 568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1099 1131 2050 2082 4997 5029 1099
Size: 32 919 32 2915 32 1550
Fragments arranged by size:
2915 1550 919 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3277 3277
Size: 5480
XbaI T'CTAG_A
Cuts at: 2511 2511
Size: 5480
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2975 2975
Size: 5480
XhoI C'TCGA_G
Cuts at: 2224 2224
Size: 5480
XmaI C'CCGG_G
Cuts at: 503 2045 2500 503
Size: 1542 455 3483
Fragments arranged by size:
3483 1542 455
XmnI GAAnn'nnTTC
Cuts at: 620 620
Size: 5480
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BfrBI
BmtI BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI
ZraI