Restriction map for
pF1KB6253
(Circular) (Six-base) MAPSORT of: pF1KB6253.seq Check: 8734 from: 1 to: 5033
pF1KB6253 5033 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1035 1035
Size: 5033
AccI GT'mk_AC
Cuts at: 2071 2071
Size: 5033
Acc65I G'GTAC_C
Cuts at: 2049 2049
Size: 5033
AclI AA'CG_TT
Cuts at: 447 4873 447
Size: 4426 607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 878 1223 1579 1696 2858 3290 4037 4305
Size: 598 345 356 117 1162 432 747 268
Cuts at: 4305 280
Size: 1008
Fragments arranged by size:
1162 1008 747 598 432 356 345 268
117
AflIII A'CryG_T
Cuts at: 903 2385 3489 4466 903
Size: 1482 1104 977 1470
Fragments arranged by size:
1482 1470 1104 977
AgeI A'CCGG_T
Cuts at: 3421 3421
Size: 5033
AlwNI CAG_nnn'CTG
Cuts at: 1928 2338 3905 1928
Size: 410 1567 3056
Fragments arranged by size:
3056 1567 410
ApaLI G'TGCA_C
Cuts at: 1133 3803 1133
Size: 2670 2363
ApoI r'AATT_y
Cuts at: 663 1279 2037 4386 4642 663
Size: 616 758 2349 256 1054
Fragments arranged by size:
2349 1054 758 616 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5033
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
AvaI C'yCGr_G
Cuts at: 239 1081 2053 239
Size: 842 972 3219
Fragments arranged by size:
3219 972 842
BamHI G'GATC_C
Cuts at: 1185 2058 1185
Size: 873 4160
BanI G'GyrC_C
Cuts at: 1246 1833 2049 2463 2711 2746 1246
Size: 587 216 414 248 35 3533
Fragments arranged by size:
3533 587 414 248 216 35
BanII G_rGCy'C
Cuts at: 377 1906 2047 3077 377
Size: 1529 141 1030 2333
Fragments arranged by size:
2333 1529 1030 141
BbeI G_GCGC'C
Cuts at: 2467 2715 2467
Size: 248 4785
BbsI GAAGACnn'nnnn_
Cuts at: 123 566 704 974 1859 123
Size: 443 138 270 885 3297
Fragments arranged by size:
3297 885 443 270 138
BbvCI CC'TCA_GC
Cuts at: 926 926
Size: 5033
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1890 1924 1890
Size: 34 4999
BciVI GTATCCnnnnn_n'
Cuts at: 2931 3692 5033 2931
Size: 761 1341 2931
Fragments arranged by size:
2931 1341 761
BclI T'GATC_A
Cuts at: 1610 2553 1610
Size: 943 4090
BfrBI ATG'CAT
Cuts at: 2002 2002
Size: 5033
BglII A'GATC_T
Cuts at: 1596 2548 4585 1596
Size: 952 2037 2044
Fragments arranged by size:
2044 2037 952
BlpI GC'TnA_GC
Cuts at: 2145 2145
Size: 5033
Bme1580I G_kGCm'C
Cuts at: 1137 1249 2658 2751 3807 1137
Size: 112 1409 93 1056 2363
Fragments arranged by size:
2363 1409 1056 112 93
BmrI ACTGGGnnnn_n'
Cuts at: 601 1752 2245 2326 2661 4805 601
Size: 1151 493 81 335 2144 829
Fragments arranged by size:
2144 1151 829 493 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3400 3400
Size: 5033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 858
Size: 5033
Bpu10I CC'TnA_GC
Cuts at: 371 779 926 371
Size: 408 147 4478
Fragments arranged by size:
4478 408 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 2209 3088 3580 3878 1520
Size: 689 879 492 298 2675
Fragments arranged by size:
2675 879 689 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1412 4737 1412
Size: 3325 1708
BsaAI yAC'GTr
Cuts at: 1995 2386 1995
Size: 391 4642
BsaBI GATnn'nnATC
Cuts at: 6 2547 4584 6
Size: 2541 2037 455
Fragments arranged by size:
2541 2037 455
BsaHI Gr'CG_yC
Cuts at: 2464 2712 3414 4910 2464
Size: 248 702 1496 2587
Fragments arranged by size:
2587 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2222 2743 3421 3695 3842 4253 2222
Size: 521 678 274 147 411 3002
Fragments arranged by size:
3002 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1972 1972
Size: 5033
BseYI C'CCAG_C
Cuts at: 1464 2458 3216 3793 4289 1464
Size: 994 758 577 496 2208
Fragments arranged by size:
2208 994 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 355
Size: 5033
BsiEI CG_ry'CG
Cuts at: 104 2575 2621 3829 104
Size: 2471 46 1208 1308
Fragments arranged by size:
2471 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 377 1137 2047 2825 3015 3807 377
Size: 760 910 778 190 792 1603
Fragments arranged by size:
1603 910 792 778 760 190
BsmI GAATG_Cn'
Cuts at: 2002 2004 2002
Size: 2 5031
Bsp1286I G_dGCh'C
Cuts at: 377 1137 1249 1906 2047 2658 2751 2825 3015
Size: 760 112 657 141 611 93 74 190
Cuts at: 3015 3077 3807 377
Size: 62 730 1603
Fragments arranged by size:
1603 760 730 657 611 190 141 112
93 74 62
BspEI T'CCGG_A
Cuts at: 4253 4253
Size: 5033
BspHI T'CATG_A
Cuts at: 4209 4209
Size: 5033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1035 1944 2083 2297 2599 2980 1035
Size: 909 139 214 302 381 3088
Fragments arranged by size:
3088 909 381 302 214 139
BsrBI CCG'CTC
Cuts at: 50 3325 3379 4863 5028 50
Size: 3275 54 1484 165 55
Fragments arranged by size:
3275 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1100 2945 1100
Size: 1845 3188
BsrFI r'CCGG_y
Cuts at: 3031 3212 3421 3031
Size: 181 209 4643
Fragments arranged by size:
4643 209 181
BsrGI T'GTAC_A
Cuts at: 426 426
Size: 5033
BssSI C'ACGA_G
Cuts at: 3304 3662 3304
Size: 358 4675
BstAPI GCAn_nnn'nTGC
Cuts at: 2410 2410
Size: 5033
BstBI TT'CG_AA
Cuts at: 3394 3394
Size: 5033
BstYI r'GATC_y
Cuts at: 1 1185 1596 2058 2525 2548 2883 3129 4141
Size: 1184 411 462 467 23 335 246 1012
Cuts at: 4141 4227 4239 4585 1
Size: 86 12 346 449
Fragments arranged by size:
1184 1012 467 462 449 411 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5033
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2983 3164 3327 2983
Size: 181 163 4689
Fragments arranged by size:
4689 181 163
BtsI GCAGTG_nn'
Cuts at: 147 343 740 2276 147
Size: 196 397 1536 2904
Fragments arranged by size:
2904 1536 397 196
DraI TTT'AAA
Cuts at: 2032 2032
Size: 5033
DrdI GACnn_nn'nnGTC
Cuts at: 2739 3597 2739
Size: 858 4175
EaeI y'GGCC_r
Cuts at: 737 813 2618 2792 3183 3210 4555 737
Size: 76 1805 174 391 27 1345 1215
Fragments arranged by size:
1805 1345 1215 391 174 76 27
EagI C'GGCC_G
Cuts at: 2618 2618
Size: 5033
EarI CTCTTCn'nnn_
Cuts at: 239 548 3056 3266 239
Size: 309 2508 210 2006
Fragments arranged by size:
2508 2006 309 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 776 3241 3551 3697 4843 776
Size: 2465 310 146 1146 966
Fragments arranged by size:
2465 1146 966 310 146
EcoICRI GAG'CTC
Cuts at: 375 2045 375
Size: 1670 3363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 858 878 1223 1579 1696 2858 3290 4037
Size: 578 20 345 356 117 1162 432 747
Cuts at: 4037 4305 280
Size: 268 1008
Fragments arranged by size:
1162 1008 747 578 432 356 345 268
117 20
EcoO109I rG'GnC_Cy
Cuts at: 385 1892 2172 385
Size: 1507 280 3246
Fragments arranged by size:
3246 1507 280
EcoRI G'AATT_C
Cuts at: 663 2037 663
Size: 1374 3659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 234 1290 3052 234
Size: 1056 1762 2215
Fragments arranged by size:
2215 1762 1056
FspI TGC'GCA
Cuts at: 2814 2814
Size: 5033
HaeII r_GCGC'y
Cuts at: 1214 2467 2715 3737 4716 1214
Size: 1253 248 1022 979 1531
Fragments arranged by size:
1531 1253 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1177 1209 1554 1566 1586 1598 1684 1716 2875
Size: 32 345 12 20 12 86 32 1159
Cuts at: 2875 2905 2907 2937 1177
Size: 30 2 30 3273
Fragments arranged by size:
3273 1159 345 86 32 32 30 30
20 12 12 2
HincII GTy'rAC
Cuts at: 828 1019 2072 828
Size: 191 1053 3789
Fragments arranged by size:
3789 1053 191
HindIII A'AGCT_T
Cuts at: 744 744
Size: 5033
HpaI GTT'AAC
Cuts at: 828 828
Size: 5033
KasI G'GCGC_C
Cuts at: 2463 2711 2463
Size: 248 4785
KpnI G_GTAC'C
Cuts at: 2053 2053
Size: 5033
MluI A'CGCG_T
Cuts at: 4466 4466
Size: 5033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 658 1729 3704 3888 658
Size: 1071 1975 184 1803
Fragments arranged by size:
1975 1803 1071 184
MscI TGG'CCA
Cuts at: 739 815 2794 739
Size: 76 1979 2978
Fragments arranged by size:
2978 1979 76
MslI CAynn'nnrTG
Cuts at: 182 959 3022 3149 182
Size: 777 2063 127 2066
Fragments arranged by size:
2066 2063 777 127
MspA1I CmG'CkG
Cuts at: 934 1183 2144 2458 2818 3831 4076 934
Size: 249 961 314 360 1013 245 1891
Fragments arranged by size:
1891 1013 961 360 314 249 245
NaeI GCC'GGC
Cuts at: 3214 3214
Size: 5033
NarI GG'CG_CC
Cuts at: 2464 2712 2464
Size: 248 4785
NcoI C'CATG_G
Cuts at: 107 107
Size: 5033
NgoMIV G'CCGG_C
Cuts at: 3212 3212
Size: 5033
NsiI A_TGCA'T
Cuts at: 2004 2004
Size: 5033
NspI r_CATG'y
Cuts at: 907 1073 1525 2086 2586 3493 907
Size: 166 452 561 500 907 2447
Fragments arranged by size:
2447 907 561 500 452 166
PciI A'CATG_T
Cuts at: 903 3489 903
Size: 2586 2447
PfoI T'CCnGG_A
Cuts at: 544 544
Size: 5033
PmeI GTTT'AAAC
Cuts at: 2032 2032
Size: 5033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 4172
Size: 32 5001
PpuMI rG'GwC_Cy
Cuts at: 385 1892 385
Size: 1507 3526
PshAI GACnn'nnGTC
Cuts at: 2020 2020
Size: 5033
PstI C_TGCA'G
Cuts at: 707 1604 2080 2765 707
Size: 897 476 685 2975
Fragments arranged by size:
2975 897 685 476
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5033
PvuII CAG'CTG
Cuts at: 934 2458 2818 934
Size: 1524 360 3149
Fragments arranged by size:
3149 1524 360
RsrII CG'GwC_CG
Cuts at: 439 3228 439
Size: 2789 2244
SacI G_AGCT'C
Cuts at: 377 2047 377
Size: 1670 3363
SalI G'TCGA_C
Cuts at: 2070 2070
Size: 5033
SapI GCTCTTCn'nnn_
Cuts at: 3056 3266 3056
Size: 210 4823
SbfI CC_TGCA'GG
Cuts at: 2080 2080
Size: 5033
ScaI AGT'ACT
Cuts at: 362 4550 362
Size: 4188 845
SfcI C'TryA_G
Cuts at: 33 703 791 893 1600 2076 2761 3754 3945
Size: 670 88 102 707 476 685 993 191
Cuts at: 3945 33
Size: 1121
Fragments arranged by size:
1121 993 707 685 670 476 191 102
88
SfoI GGC'GCC
Cuts at: 2465 2713 2465
Size: 248 4785
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
SmaI CCC'GGG
Cuts at: 241 1083 2055 241
Size: 842 972 3219
Fragments arranged by size:
3219 972 842
SmlI C'TyrA_G
Cuts at: 1535 2188 3103 3595 3857 1535
Size: 653 915 492 262 2711
Fragments arranged by size:
2711 915 653 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5033
SphI G_CATG'C
Cuts at: 2086 2586 2086
Size: 500 4533
SspI AAT'ATT
Cuts at: 1809 1809
Size: 5033
StyI C'CwwG_G
Cuts at: 107 388 2167 2521 107
Size: 281 1779 354 2619
Fragments arranged by size:
2619 1779 354 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2616 2749 3208 3417 2616
Size: 133 459 209 4232
Fragments arranged by size:
4232 459 209 133
TatI w'GTAC_w
Cuts at: 360 426 4548 360
Size: 66 4122 845
Fragments arranged by size:
4122 845 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1031 1063 4550 4582 1031
Size: 32 3487 32 1482
Fragments arranged by size:
3487 1482 32 32
Tth111I GACn'n_nGTC
Cuts at: 2830 2830
Size: 5033
XbaI T'CTAG_A
Cuts at: 2064 2064
Size: 5033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 184 2528 184
Size: 2344 2689
XmaI C'CCGG_G
Cuts at: 239 1081 2053 239
Size: 842 972 3219
Fragments arranged by size:
3219 972 842
XmnI GAAnn'nnTTC
Cuts at: 2002 2002
Size: 5033
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI