Restriction map for
pF1KB6241
(Circular) (Six-base) MAPSORT of: pF1KB6241.seq Check: 2842 from: 1 to: 5366
pF1KB6241 5366 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 1367 2404 1367
Size: 1037 4329
Acc65I G'GTAC_C
Cuts at: 2382 2382
Size: 5366
AclI AA'CG_TT
Cuts at: 1650 5206 1650
Size: 3556 1810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 657 825 2085 3191 3623 4370 4638 482
Size: 175 168 1260 1106 432 747 268 1210
Fragments arranged by size:
1260 1210 1106 747 432 268 175 168
AflIII A'CryG_T
Cuts at: 372 2718 3822 4799 372
Size: 2346 1104 977 939
Fragments arranged by size:
2346 1104 977 939
AgeI A'CCGG_T
Cuts at: 3754 3754
Size: 5366
AleI CACnn'nnGTG
Cuts at: 1640 2315 1640
Size: 675 4691
AlwNI CAG_nnn'CTG
Cuts at: 2671 4238 2671
Size: 1567 3799
ApaI G_GGCC'C
Cuts at: 213 213
Size: 5366
ApaLI G'TGCA_C
Cuts at: 1117 4136 1117
Size: 3019 2347
ApoI r'AATT_y
Cuts at: 263 627 2370 4719 4975 263
Size: 364 1743 2349 256 654
Fragments arranged by size:
2349 1743 654 364 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5366
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5366
AvaI C'yCGr_G
Cuts at: 1806 2386 1806
Size: 580 4786
BamHI G'GATC_C
Cuts at: 2391 2391
Size: 5366
BanI G'GyrC_C
Cuts at: 2382 2796 3044 3079 2382
Size: 414 248 35 4669
Fragments arranged by size:
4669 414 248 35
BanII G_rGCy'C
Cuts at: 199 213 2380 3410 199
Size: 14 2167 1030 2155
Fragments arranged by size:
2167 2155 1030 14
BbeI G_GCGC'C
Cuts at: 2800 3048 2800
Size: 248 5118
BbsI GAAGACnn'nnnn_
Cuts at: 339 1888 339
Size: 1549 3817
BciVI GTATCCnnnnn_n'
Cuts at: 2132 3264 4025 5366 2132
Size: 1132 761 1341 2132
Fragments arranged by size:
2132 1341 1132 761
BclI T'GATC_A
Cuts at: 435 2886 435
Size: 2451 2915
BglI GCCn_nnn'nGGC
Cuts at: 344 344
Size: 5366
BglII A'GATC_T
Cuts at: 2881 4918 2881
Size: 2037 3329
BlpI GC'TnA_GC
Cuts at: 193 2478 193
Size: 2285 3081
Bme1580I G_kGCm'C
Cuts at: 213 317 1121 1635 2991 3084 4140 213
Size: 104 804 514 1356 93 1056 1439
Fragments arranged by size:
1439 1356 1056 804 514 104 93
BmrI ACTGGGnnnn_n'
Cuts at: 2578 2659 2994 5138 2578
Size: 81 335 2144 2806
Fragments arranged by size:
2806 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 218 3733 218
Size: 3515 1851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 253
Size: 5366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 832 2542 3421 3913 4211 519
Size: 313 1710 879 492 298 1674
Fragments arranged by size:
1710 1674 879 492 313 298
BsaI GGTCTCn'nnnn_
Cuts at: 5070 5070
Size: 5366
BsaAI yAC'GTr
Cuts at: 2317 2719 2317
Size: 402 4964
BsaBI GATnn'nnATC
Cuts at: 6 691 2880 4917 6
Size: 685 2189 2037 455
Fragments arranged by size:
2189 2037 685 455
BsaHI Gr'CG_yC
Cuts at: 336 2797 3045 3747 5243 336
Size: 2461 248 702 1496 459
Fragments arranged by size:
2461 1496 702 459 248
BsaWI w'CCGG_w
Cuts at: 2555 3076 3754 4028 4175 4586 2555
Size: 521 678 274 147 411 3335
Fragments arranged by size:
3335 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 534 564 2331 2361 534
Size: 30 1767 30 3539
Fragments arranged by size:
3539 1767 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 555 850 1270 1489 1573 1858 2026 555
Size: 295 420 219 84 285 168 3895
Fragments arranged by size:
3895 420 295 285 219 168 84
BseYI C'CCAG_C
Cuts at: 2791 3549 4126 4622 2791
Size: 758 577 496 3535
Fragments arranged by size:
3535 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 674 840 1260 374
Size: 300 166 420 4480
Fragments arranged by size:
4480 420 300 166
BsiEI CG_ry'CG
Cuts at: 104 2908 2954 4162 104
Size: 2804 46 1208 1308
Fragments arranged by size:
2804 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1121 2380 3158 3348 4140 1121
Size: 1259 778 190 792 2347
Fragments arranged by size:
2347 1259 792 778 190
BsmI GAATG_Cn'
Cuts at: 643 991 1047 2254 643
Size: 348 56 1207 3755
Fragments arranged by size:
3755 1207 348 56
Bsp1286I G_dGCh'C
Cuts at: 199 213 317 1121 1635 2380 2991 3084 3158
Size: 14 104 804 514 745 611 93 74
Cuts at: 3158 3348 3410 4140 199
Size: 190 62 730 1425
Fragments arranged by size:
1425 804 745 730 611 514 190 104
93 74 62 14
BspEI T'CCGG_A
Cuts at: 4586 4586
Size: 5366
BspHI T'CATG_A
Cuts at: 4542 4542
Size: 5366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2416 2630 2932 3313 2416
Size: 214 302 381 4469
Fragments arranged by size:
4469 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3658 3712 5196 5361 50
Size: 3608 54 1484 165 55
Fragments arranged by size:
3608 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 772 3278 772
Size: 2506 2860
BsrFI r'CCGG_y
Cuts at: 338 3364 3545 3754 338
Size: 3026 181 209 1950
Fragments arranged by size:
3026 1950 209 181
BsrGI T'GTAC_A
Cuts at: 215 2332 215
Size: 2117 3249
BssSI C'ACGA_G
Cuts at: 3637 3995 3637
Size: 358 5008
BstAPI GCAn_nnn'nTGC
Cuts at: 1835 2743 1835
Size: 908 4458
BstBI TT'CG_AA
Cuts at: 3727 3727
Size: 5366
BstXI CCAn_nnnn'nTGG
Cuts at: 997 1165 2260 997
Size: 168 1095 4103
Fragments arranged by size:
4103 1095 168
BstYI r'GATC_y
Cuts at: 1 2391 2858 2881 3216 3462 4474 4560 4572
Size: 2390 467 23 335 246 1012 86 12
Cuts at: 4572 4918 1
Size: 346 449
Fragments arranged by size:
2390 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 116 1627 116
Size: 1511 3855
BtgI C'CryG_G
Cuts at: 107 494 107
Size: 387 4979
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3316 3497 3660 3316
Size: 181 163 5022
Fragments arranged by size:
5022 181 163
BtsI GCAGTG_nn'
Cuts at: 828 1252 1756 2609 828
Size: 424 504 853 3585
Fragments arranged by size:
3585 853 504 424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 894 929 1062 1097 1482 1517 1986 2021 894
Size: 35 133 35 385 35 469 35 4239
Fragments arranged by size:
4239 469 385 133 35 35 35 35
DraI TTT'AAA
Cuts at: 2365 2365
Size: 5366
DrdI GACnn_nn'nnGTC
Cuts at: 3072 3930 3072
Size: 858 4508
EaeI y'GGCC_r
Cuts at: 340 1167 2951 3125 3516 3543 4888 340
Size: 827 1784 174 391 27 1345 818
Fragments arranged by size:
1784 1345 827 818 391 174 27
EagI C'GGCC_G
Cuts at: 2951 2951
Size: 5366
EarI CTCTTCn'nnn_
Cuts at: 320 1876 2104 3389 3599 320
Size: 1556 228 1285 210 2087
Fragments arranged by size:
2087 1556 1285 228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2151 3574 3884 4030 5176 2151
Size: 1423 310 146 1146 2341
Fragments arranged by size:
2341 1423 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2378 2378
Size: 5366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 482 657 825 2085 3191 3623 4370 4638
Size: 229 175 168 1260 1106 432 747 268
Cuts at: 4638 253
Size: 981
Fragments arranged by size:
1260 1106 981 747 432 268 229 175
168
EcoO109I rG'GnC_Cy
Cuts at: 112 209 210 346 2505 112
Size: 97 1 136 2159 2973
Fragments arranged by size:
2973 2159 136 97 1
EcoRI G'AATT_C
Cuts at: 263 2370 263
Size: 2107 3259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1125 1545 2328 3385 1125
Size: 420 783 1057 3106
Fragments arranged by size:
3106 1057 783 420
FspI TGC'GCA
Cuts at: 3147 3147
Size: 5366
HaeII r_GCGC'y
Cuts at: 2800 3048 4070 5049 2800
Size: 248 1022 979 3117
Fragments arranged by size:
3117 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 573 605 1006 1038 1499 1531 1594 1626 1835
Size: 32 401 32 461 32 63 32 209
Cuts at: 1835 1867 2341 2373 3208 3238 3240 3270 573
Size: 32 474 32 835 30 2 30 2669
Fragments arranged by size:
2669 835 474 461 401 209 63 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1358 1442 1526 2405 1358
Size: 84 84 879 4319
Fragments arranged by size:
4319 879 84 84
HindIII A'AGCT_T
Cuts at: 441 1341 441
Size: 900 4466
KasI G'GCGC_C
Cuts at: 2796 3044 2796
Size: 248 5118
KpnI G_GTAC'C
Cuts at: 2386 2386
Size: 5366
MluI A'CGCG_T
Cuts at: 4799 4799
Size: 5366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4037 4221 4037
Size: 184 5182
MscI TGG'CCA
Cuts at: 3127 3127
Size: 5366
MslI CAynn'nnrTG
Cuts at: 648 1640 2315 3355 3482 648
Size: 992 675 1040 127 2532
Fragments arranged by size:
2532 1040 992 675 127
MspA1I CmG'CkG
Cuts at: 460 2105 2477 2791 3151 4164 4409 460
Size: 1645 372 314 360 1013 245 1417
Fragments arranged by size:
1645 1417 1013 372 360 314 245
NaeI GCC'GGC
Cuts at: 340 3547 340
Size: 3207 2159
NarI GG'CG_CC
Cuts at: 2797 3045 2797
Size: 248 5118
NcoI C'CATG_G
Cuts at: 107 494 107
Size: 387 4979
NgoMIV G'CCGG_C
Cuts at: 338 3545 338
Size: 3207 2159
NspI r_CATG'y
Cuts at: 376 526 737 2419 2919 3826 376
Size: 150 211 1682 500 907 1916
Fragments arranged by size:
1916 1682 907 500 211 150
PasI CC'CwG_GG
Cuts at: 317 317
Size: 5366
PciI A'CATG_T
Cuts at: 372 3822 372
Size: 3450 1916
PmeI GTTT'AAAC
Cuts at: 2365 2365
Size: 5366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 4505
Size: 32 5334
PpuMI rG'GwC_Cy
Cuts at: 112 112
Size: 5366
PspOMI G'GGCC_C
Cuts at: 209 209
Size: 5366
PstI C_TGCA'G
Cuts at: 1663 2413 3098 1663
Size: 750 685 3931
Fragments arranged by size:
3931 750 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5366
PvuII CAG'CTG
Cuts at: 460 2105 2791 3151 460
Size: 1645 686 360 2675
Fragments arranged by size:
2675 1645 686 360
RsrII CG'GwC_CG
Cuts at: 3561 3561
Size: 5366
SacI G_AGCT'C
Cuts at: 2380 2380
Size: 5366
SalI G'TCGA_C
Cuts at: 2403 2403
Size: 5366
SapI GCTCTTCn'nnn_
Cuts at: 3389 3599 3389
Size: 210 5156
SbfI CC_TGCA'GG
Cuts at: 2413 2413
Size: 5366
ScaI AGT'ACT
Cuts at: 4883 4883
Size: 5366
SfcI C'TryA_G
Cuts at: 33 240 593 1659 1911 2289 2409 3094 4087
Size: 207 353 1066 252 378 120 685 993
Cuts at: 4087 4278 33
Size: 191 1121
Fragments arranged by size:
1121 1066 993 685 378 353 252 207
191 120
SfoI GGC'GCC
Cuts at: 2798 3046 2798
Size: 248 5118
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5366
SmaI CCC'GGG
Cuts at: 2388 2388
Size: 5366
SmlI C'TyrA_G
Cuts at: 534 847 2521 3436 3928 4190 534
Size: 313 1674 915 492 262 1710
Fragments arranged by size:
1710 1674 915 492 313 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5366
SphI G_CATG'C
Cuts at: 2419 2919 2419
Size: 500 4866
StuI AGG'CCT
Cuts at: 2241 2241
Size: 5366
StyI C'CwwG_G
Cuts at: 107 494 2500 2854 107
Size: 387 2006 354 2619
Fragments arranged by size:
2619 2006 387 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 187 1630 2335 2949 3082 3541 3750 187
Size: 1443 705 614 133 459 209 1803
Fragments arranged by size:
1803 1443 705 614 459 209 133
TatI w'GTAC_w
Cuts at: 215 2332 4881 215
Size: 2117 2549 700
Fragments arranged by size:
2549 2117 700
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 534 566 2331 2363 4883 4915 534
Size: 32 1765 32 2520 32 985
Fragments arranged by size:
2520 1765 985 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3163 3163
Size: 5366
XbaI T'CTAG_A
Cuts at: 2397 2397
Size: 5366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2861 2861
Size: 5366
XmaI C'CCGG_G
Cuts at: 2386 2386
Size: 5366
XmnI GAAnn'nnTTC
Cuts at: 1735 1903 2071 1735
Size: 168 168 5030
Fragments arranged by size:
5030 168 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmBI BssHII BstEII BstZ17I ClaI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI