Restriction map for
pF1KB6239
(Circular) (Six-base) MAPSORT of: pF1KB6239.seq Check: 1610 from: 1 to: 5366
pF1KB6239 5366 bp
With 182 enzymes: SgfI *
January 18, 2013 16:22 ..
AccI GT'mk_AC
Cuts at: 1512 2404 1512
Size: 892 4474
Acc65I G'GTAC_C
Cuts at: 2382 2382
Size: 5366
AclI AA'CG_TT
Cuts at: 5206 5206
Size: 5366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 707 1367 1448 2344 3191 3623 4370 4638
Size: 300 660 81 896 847 432 747 268
Cuts at: 4638 407
Size: 1135
Fragments arranged by size:
1135 896 847 747 660 432 300 268
81
AflIII A'CryG_T
Cuts at: 1507 2105 2718 3822 4799 1507
Size: 598 613 1104 977 2074
Fragments arranged by size:
2074 1104 977 613 598
AgeI A'CCGG_T
Cuts at: 1610 3754 1610
Size: 2144 3222
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 845 877 845
Size: 32 5334
AlwNI CAG_nnn'CTG
Cuts at: 1279 2671 4238 1279
Size: 1392 1567 2407
Fragments arranged by size:
2407 1567 1392
ApaLI G'TGCA_C
Cuts at: 310 4136 310
Size: 3826 1540
ApoI r'AATT_y
Cuts at: 251 462 614 757 773 1353 1743 2029 2171
Size: 211 152 143 16 580 390 286 142
Cuts at: 2171 2370 4719 4975 251
Size: 199 2349 256 642
Fragments arranged by size:
2349 642 580 390 286 256 211 199
152 143 142 16
AseI AT'TA_AT
Cuts at: 20 1232 20
Size: 1212 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5366
AvaI C'yCGr_G
Cuts at: 2386 2386
Size: 5366
BamHI G'GATC_C
Cuts at: 2391 2391
Size: 5366
BanI G'GyrC_C
Cuts at: 1971 2382 2796 3044 3079 1971
Size: 411 414 248 35 4258
Fragments arranged by size:
4258 414 411 248 35
BanII G_rGCy'C
Cuts at: 334 1342 1951 2380 3410 334
Size: 1008 609 429 1030 2290
Fragments arranged by size:
2290 1030 1008 609 429
BbeI G_GCGC'C
Cuts at: 2800 3048 2800
Size: 248 5118
BbvCI CC'TCA_GC
Cuts at: 1568 1568
Size: 5366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2171 2205 2171
Size: 34 5332
BciVI GTATCCnnnnn_n'
Cuts at: 293 1245 1572 3264 4025 5366 293
Size: 952 327 1692 761 1341 293
Fragments arranged by size:
1692 1341 952 761 327 293
BclI T'GATC_A
Cuts at: 1692 2235 2886 1692
Size: 543 651 4172
Fragments arranged by size:
4172 651 543
BglII A'GATC_T
Cuts at: 711 2881 4918 711
Size: 2170 2037 1159
Fragments arranged by size:
2170 2037 1159
BlpI GC'TnA_GC
Cuts at: 1142 2478 1142
Size: 1336 4030
Bme1580I G_kGCm'C
Cuts at: 314 814 1726 1837 2991 3084 4140 314
Size: 500 912 111 1154 93 1056 1540
Fragments arranged by size:
1540 1154 1056 912 500 111 93
BmgBI CAC'GTC
Cuts at: 1983 1983
Size: 5366
BmrI ACTGGGnnnn_n'
Cuts at: 128 2578 2659 2994 5138 128
Size: 2450 81 335 2144 356
Fragments arranged by size:
2450 2144 356 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3733 3733
Size: 5366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1541 736
Size: 805 4561
Bpu10I CC'TnA_GC
Cuts at: 1568 1568
Size: 5366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 2048 2542 3421 3913 4211 178
Size: 1870 494 879 492 298 1333
Fragments arranged by size:
1870 1333 879 494 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 452 5070 452
Size: 4618 748
BsaAI yAC'GTr
Cuts at: 2719 2719
Size: 5366
BsaBI GATnn'nnATC
Cuts at: 6 2002 2880 4917 6
Size: 1996 878 2037 455
Fragments arranged by size:
2037 1996 878 455
BsaHI Gr'CG_yC
Cuts at: 2797 3045 3747 5243 2797
Size: 248 702 1496 2920
Fragments arranged by size:
2920 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1610 2555 3076 3754 4028 4175 4586 1610
Size: 945 521 678 274 147 411 2390
Fragments arranged by size:
2390 945 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 503 533 845 875 503
Size: 30 312 30 4994
Fragments arranged by size:
4994 312 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1332 1574 1650 1332
Size: 242 76 5048
Fragments arranged by size:
5048 242 76
BseYI C'CCAG_C
Cuts at: 1216 1991 2791 3549 4126 4622 1216
Size: 775 800 758 577 496 1960
Fragments arranged by size:
1960 800 775 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2038 2130 1607
Size: 431 92 4843
Fragments arranged by size:
4843 431 92
BsiEI CG_ry'CG
Cuts at: 104 2908 2954 4162 104
Size: 2804 46 1208 1308
Fragments arranged by size:
2804 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 314 334 1342 2380 3158 3348 4140 314
Size: 20 1008 1038 778 190 792 1540
Fragments arranged by size:
1540 1038 1008 792 778 190 20
BsmI GAATG_Cn'
Cuts at: 417 639 417
Size: 222 5144
BsmBI CGTCTCn'nnnn_
Cuts at: 671 671
Size: 5366
Bsp1286I G_dGCh'C
Cuts at: 314 334 814 1342 1726 1837 1951 2380 2991
Size: 20 480 528 384 111 114 429 611
Cuts at: 2991 3084 3158 3348 3410 4140 314
Size: 93 74 190 62 730 1540
Fragments arranged by size:
1540 730 611 528 480 429 384 190
114 111 93 74 62 20
BspEI T'CCGG_A
Cuts at: 4586 4586
Size: 5366
BspHI T'CATG_A
Cuts at: 4542 4542
Size: 5366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2416 2630 2932 3313 2416
Size: 214 302 381 4469
Fragments arranged by size:
4469 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1146 3658 3712 5196 5361 50
Size: 1096 2512 54 1484 165 55
Fragments arranged by size:
2512 1484 1096 165 55 54
BsrDI GCAATG_nn'
Cuts at: 334 1481 3278 334
Size: 1147 1797 2422
Fragments arranged by size:
2422 1797 1147
BsrFI r'CCGG_y
Cuts at: 1610 3364 3545 3754 1610
Size: 1754 181 209 3222
Fragments arranged by size:
3222 1754 209 181
BssSI C'ACGA_G
Cuts at: 3637 3995 3637
Size: 358 5008
BstAPI GCAn_nnn'nTGC
Cuts at: 1931 1965 2743 1931
Size: 34 778 4554
Fragments arranged by size:
4554 778 34
BstBI TT'CG_AA
Cuts at: 3727 3727
Size: 5366
BstEII G'GTnAC_C
Cuts at: 360 360
Size: 5366
BstXI CCAn_nnnn'nTGG
Cuts at: 149 372 149
Size: 223 5143
BstYI r'GATC_y
Cuts at: 1 711 2010 2391 2858 2881 3216 3462 4474
Size: 710 1299 381 467 23 335 246 1012
Cuts at: 4474 4560 4572 4918 1
Size: 86 12 346 449
Fragments arranged by size:
1299 1012 710 467 449 381 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1699 107
Size: 1592 3774
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 984 3316 3497 3660 984
Size: 2332 181 163 2690
Fragments arranged by size:
2690 2332 181 163
BtsI GCAGTG_nn'
Cuts at: 1626 1837 2609 1626
Size: 211 772 4383
Fragments arranged by size:
4383 772 211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1062 1097 1062
Size: 35 5331
DraI TTT'AAA
Cuts at: 978 2365 978
Size: 1387 3979
DrdI GACnn_nn'nnGTC
Cuts at: 3072 3930 3072
Size: 858 4508
EaeI y'GGCC_r
Cuts at: 2951 3125 3516 3543 4888 2951
Size: 174 391 27 1345 3429
Fragments arranged by size:
3429 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2951 2951
Size: 5366
EarI CTCTTCn'nnn_
Cuts at: 1179 1310 1594 2076 2276 3389 3599 1179
Size: 131 284 482 200 1113 210 2946
Fragments arranged by size:
2946 1113 482 284 210 200 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 168 3574 3884 4030 5176 168
Size: 3406 310 146 1146 358
Fragments arranged by size:
3406 1146 358 310 146
EcoICRI GAG'CTC
Cuts at: 332 1340 2378 332
Size: 1008 1038 3320
Fragments arranged by size:
3320 1038 1008
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 707 736 1367 1448 1541 2344 3191 3623
Size: 300 29 631 81 93 803 847 432
Cuts at: 3623 4370 4638 407
Size: 747 268 1135
Fragments arranged by size:
1135 847 803 747 631 432 300 268
93 81 29
EcoO109I rG'GnC_Cy
Cuts at: 2505 2505
Size: 5366
EcoRI G'AATT_C
Cuts at: 2029 2370 2029
Size: 341 5025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3385 3385
Size: 5366
FspI TGC'GCA
Cuts at: 3147 3147
Size: 5366
HaeII r_GCGC'y
Cuts at: 2800 3048 4070 5049 2800
Size: 248 1022 979 3117
Fragments arranged by size:
3117 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1645 1677 2046 2078 3208 3238 3240 3270 1645
Size: 32 369 32 1130 30 2 30 3741
Fragments arranged by size:
3741 1130 369 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2229 2405 2229
Size: 176 5190
HindIII A'AGCT_T
Cuts at: 936 936
Size: 5366
HpaI GTT'AAC
Cuts at: 2229 2229
Size: 5366
KasI G'GCGC_C
Cuts at: 2796 3044 2796
Size: 248 5118
KpnI G_GTAC'C
Cuts at: 2386 2386
Size: 5366
MluI A'CGCG_T
Cuts at: 4799 4799
Size: 5366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 406 4037 4221 406
Size: 3631 184 1551
Fragments arranged by size:
3631 1551 184
MscI TGG'CCA
Cuts at: 3127 3127
Size: 5366
MslI CAynn'nnrTG
Cuts at: 728 1234 3355 3482 728
Size: 506 2121 127 2612
Fragments arranged by size:
2612 2121 506 127
MspA1I CmG'CkG
Cuts at: 1141 1567 1644 1805 1894 1944 2477 2791 3151
Size: 426 77 161 89 50 533 314 360
Cuts at: 3151 4164 4409 1141
Size: 1013 245 2098
Fragments arranged by size:
2098 1013 533 426 360 314 245 161
89 77 50
NaeI GCC'GGC
Cuts at: 3547 3547
Size: 5366
NarI GG'CG_CC
Cuts at: 2797 3045 2797
Size: 248 5118
NcoI C'CATG_G
Cuts at: 107 107
Size: 5366
NgoMIV G'CCGG_C
Cuts at: 3545 3545
Size: 5366
NspI r_CATG'y
Cuts at: 180 1511 2109 2419 2919 3826 180
Size: 1331 598 310 500 907 1720
Fragments arranged by size:
1720 1331 907 598 500 310
PciI A'CATG_T
Cuts at: 1507 2105 3822 1507
Size: 598 1717 3051
Fragments arranged by size:
3051 1717 598
PflMI CCAn_nnn'nTGG
Cuts at: 1104 1104
Size: 5366
PfoI T'CCnGG_A
Cuts at: 1170 1794 1170
Size: 624 4742
PmeI GTTT'AAAC
Cuts at: 2365 2365
Size: 5366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 845 877 4505 4537 845
Size: 32 3628 32 1674
Fragments arranged by size:
3628 1674 32 32
PshAI GACnn'nnGTC
Cuts at: 1874 1874
Size: 5366
PsiI TTA'TAA
Cuts at: 604 1040 604
Size: 436 4930
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 219 251 219
Size: 32 5334
PstI C_TGCA'G
Cuts at: 1633 1936 2413 3098 1633
Size: 303 477 685 3901
Fragments arranged by size:
3901 685 477 303
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5366
PvuII CAG'CTG
Cuts at: 1644 2791 3151 1644
Size: 1147 360 3859
Fragments arranged by size:
3859 1147 360
RsrII CG'GwC_CG
Cuts at: 3561 3561
Size: 5366
SacI G_AGCT'C
Cuts at: 334 1342 2380 334
Size: 1008 1038 3320
Fragments arranged by size:
3320 1038 1008
SalI G'TCGA_C
Cuts at: 2403 2403
Size: 5366
SapI GCTCTTCn'nnn_
Cuts at: 2276 3389 3599 2276
Size: 1113 210 4043
Fragments arranged by size:
4043 1113 210
SbfI CC_TGCA'GG
Cuts at: 2413 2413
Size: 5366
ScaI AGT'ACT
Cuts at: 4883 4883
Size: 5366
SfcI C'TryA_G
Cuts at: 33 454 963 1629 1932 2409 3094 4087 4278
Size: 421 509 666 303 477 685 993 191
Cuts at: 4278 33
Size: 1121
Fragments arranged by size:
1121 993 685 666 509 477 421 303
191
SfoI GGC'GCC
Cuts at: 2798 3046 2798
Size: 248 5118
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5366
SmaI CCC'GGG
Cuts at: 2388 2388
Size: 5366
SmlI C'TyrA_G
Cuts at: 193 2063 2521 3436 3928 4190 193
Size: 1870 458 915 492 262 1369
Fragments arranged by size:
1870 1369 915 492 458 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5366
SphI G_CATG'C
Cuts at: 2419 2919 2419
Size: 500 4866
SspI AAT'ATT
Cuts at: 1392 1392
Size: 5366
StyI C'CwwG_G
Cuts at: 107 639 1588 2500 2854 107
Size: 532 949 912 354 2619
Fragments arranged by size:
2619 949 912 532 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1044 2949 3082 3541 3750 1044
Size: 1905 133 459 209 2660
Fragments arranged by size:
2660 1905 459 209 133
TatI w'GTAC_w
Cuts at: 173 576 4881 173
Size: 403 4305 658
Fragments arranged by size:
4305 658 403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 377 409 4883 4915 377
Size: 32 4474 32 828
Fragments arranged by size:
4474 828 32 32
Tth111I GACn'n_nGTC
Cuts at: 3163 3163
Size: 5366
XbaI T'CTAG_A
Cuts at: 1065 2397 1065
Size: 1332 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1236 2861 1236
Size: 1625 3741
XmaI C'CCGG_G
Cuts at: 2386 2386
Size: 5366
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmtI BsiWI
BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI