Restriction map for
pF1KB6237
(Circular) (Six-base) MAPSORT of: pF1KB6237.seq Check: 6242 from: 1 to: 5366
pF1KB6237 5366 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 2404 2404
Size: 5366
Acc65I G'GTAC_C
Cuts at: 394 2382 394
Size: 1988 3378
AclI AA'CG_TT
Cuts at: 5206 5206
Size: 5366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 867 1371 1539 1791 2295 3191 3623 4370
Size: 388 504 168 252 504 896 432 747
Cuts at: 4370 4638 479
Size: 268 1207
Fragments arranged by size:
1207 896 747 504 504 432 388 268
252 168
AflIII A'CryG_T
Cuts at: 2718 3822 4799 2718
Size: 1104 977 3285
Fragments arranged by size:
3285 1104 977
AgeI A'CCGG_T
Cuts at: 3754 3754
Size: 5366
AlwNI CAG_nnn'CTG
Cuts at: 448 585 1562 2346 2671 4238 448
Size: 137 977 784 325 1567 1576
Fragments arranged by size:
1576 1567 977 784 325 137
ApaI G_GGCC'C
Cuts at: 1313 1313
Size: 5366
ApaLI G'TGCA_C
Cuts at: 4136 4136
Size: 5366
ApoI r'AATT_y
Cuts at: 652 741 917 1001 1085 1169 1253 1505 1589
Size: 89 176 84 84 84 84 252 84
Cuts at: 1589 1757 1925 2093 2261 2370 4719 4975 652
Size: 168 168 168 168 109 2349 256 1043
Fragments arranged by size:
2349 1043 256 252 176 168 168 168
168 109 89 84 84 84 84 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 5366
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5366
AvaI C'yCGr_G
Cuts at: 2386 2386
Size: 5366
BamHI G'GATC_C
Cuts at: 2391 2391
Size: 5366
BanI G'GyrC_C
Cuts at: 394 2382 2796 3044 3079 394
Size: 1988 414 248 35 2681
Fragments arranged by size:
2681 1988 414 248 35
BanII G_rGCy'C
Cuts at: 1313 2071 2380 3410 1313
Size: 758 309 1030 3269
Fragments arranged by size:
3269 1030 758 309
BbeI G_GCGC'C
Cuts at: 2800 3048 2800
Size: 248 5118
BciVI GTATCCnnnnn_n'
Cuts at: 3264 4025 5366 3264
Size: 761 1341 3264
Fragments arranged by size:
3264 1341 761
BclI T'GATC_A
Cuts at: 948 2886 948
Size: 1938 3428
BglI GCCn_nnn'nGGC
Cuts at: 1145 1145
Size: 5366
BglII A'GATC_T
Cuts at: 114 2881 4918 114
Size: 2767 2037 562
Fragments arranged by size:
2767 2037 562
BlpI GC'TnA_GC
Cuts at: 1745 2478 1745
Size: 733 4633
Bme1580I G_kGCm'C
Cuts at: 1313 2991 3084 4140 1313
Size: 1678 93 1056 2539
Fragments arranged by size:
2539 1678 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 336 417 617 745 1439 2111 2578 2659 2994
Size: 81 200 128 694 672 467 81 335
Cuts at: 2994 5138 336
Size: 2144 564
Fragments arranged by size:
2144 694 672 564 467 335 200 128
81 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1754 3733 1754
Size: 1979 3387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 458 689 863 947 1031 1283 1535 1787
Size: 348 231 174 84 84 252 252 252
Cuts at: 1787 1871 2207 110
Size: 84 336 3269
Fragments arranged by size:
3269 348 336 252 252 252 231 174
84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1667 2542 3421 3913 4211 1667
Size: 875 879 492 298 2822
Fragments arranged by size:
2822 879 875 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 306 706 5070 306
Size: 400 4364 602
Fragments arranged by size:
4364 602 400
BsaAI yAC'GTr
Cuts at: 2719 2719
Size: 5366
BsaBI GATnn'nnATC
Cuts at: 6 2880 4917 6
Size: 2874 2037 455
Fragments arranged by size:
2874 2037 455
BsaHI Gr'CG_yC
Cuts at: 2797 3045 3747 5243 2797
Size: 248 702 1496 2920
Fragments arranged by size:
2920 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2555 3076 3754 4028 4175 4586 2555
Size: 521 678 274 147 411 3335
Fragments arranged by size:
3335 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2791 3549 4126 4622 2791
Size: 758 577 496 3535
Fragments arranged by size:
3535 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 1472 422
Size: 1050 4316
BsiEI CG_ry'CG
Cuts at: 104 2908 2954 4162 104
Size: 2804 46 1208 1308
Fragments arranged by size:
2804 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1231 2071 2155 2239 2380 3158 3348 4140 1231
Size: 840 84 84 141 778 190 792 2457
Fragments arranged by size:
2457 840 792 778 190 141 84 84
BsiWI C'GTAC_G
Cuts at: 1358 1358
Size: 5366
BsmI GAATG_Cn'
Cuts at: 383 1369 1621 1888 2218 383
Size: 986 252 267 330 3531
Fragments arranged by size:
3531 986 330 267 252
Bsp1286I G_dGCh'C
Cuts at: 1231 1313 2071 2155 2239 2380 2991 3084 3158
Size: 82 758 84 84 141 611 93 74
Cuts at: 3158 3348 3410 4140 1231
Size: 190 62 730 2457
Fragments arranged by size:
2457 758 730 611 190 141 93 84
84 82 74 62
BspEI T'CCGG_A
Cuts at: 4586 4586
Size: 5366
BspHI T'CATG_A
Cuts at: 1788 4542 1788
Size: 2754 2612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2416 2630 2932 3313 2416
Size: 214 302 381 4469
Fragments arranged by size:
4469 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3658 3712 5196 5361 50
Size: 3608 54 1484 165 55
Fragments arranged by size:
3608 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 786 1374 1542 2130 3278 786
Size: 588 168 588 1148 2874
Fragments arranged by size:
2874 1148 588 588 168
BsrFI r'CCGG_y
Cuts at: 3364 3545 3754 3364
Size: 181 209 4976
Fragments arranged by size:
4976 209 181
BssSI C'ACGA_G
Cuts at: 1596 3637 3995 1596
Size: 2041 358 2967
Fragments arranged by size:
2967 2041 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2743 2743
Size: 5366
BstBI TT'CG_AA
Cuts at: 3727 3727
Size: 5366
BstEII G'GTnAC_C
Cuts at: 603 603
Size: 5366
BstYI r'GATC_y
Cuts at: 1 114 1841 2391 2858 2881 3216 3462 4474
Size: 113 1727 550 467 23 335 246 1012
Cuts at: 4474 4560 4572 4918 1
Size: 86 12 346 449
Fragments arranged by size:
1727 1012 550 467 449 346 335 246
113 86 23 12
BtgI C'CryG_G
Cuts at: 107 364 107
Size: 257 5109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3316 3497 3660 3316
Size: 181 163 5022
Fragments arranged by size:
5022 181 163
BtsI GCAGTG_nn'
Cuts at: 583 1626 2609 583
Size: 1043 983 3340
Fragments arranged by size:
3340 1043 983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 768 803 1356 1391 1524 1559 768
Size: 35 553 35 133 35 4575
Fragments arranged by size:
4575 553 133 35 35 35
DraI TTT'AAA
Cuts at: 1194 2365 1194
Size: 1171 4195
DrdI GACnn_nn'nnGTC
Cuts at: 3072 3930 3072
Size: 858 4508
EaeI y'GGCC_r
Cuts at: 249 2951 3125 3516 3543 4888 249
Size: 2702 174 391 27 1345 727
Fragments arranged by size:
2702 1345 727 391 174 27
EagI C'GGCC_G
Cuts at: 2951 2951
Size: 5366
EarI CTCTTCn'nnn_
Cuts at: 458 704 3389 3599 458
Size: 246 2685 210 2225
Fragments arranged by size:
2685 2225 246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3574 3884 4030 5176 3574
Size: 310 146 1146 3764
Fragments arranged by size:
3764 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2069 2378 2069
Size: 309 5057
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 458 479 689 863 867 947 1031 1283
Size: 348 21 210 174 4 80 84 252
Cuts at: 1283 1371 1535 1539 1787 1791 1871 2207 2295
Size: 88 164 4 248 4 80 336 88
Cuts at: 2295 3191 3623 4370 4638 110
Size: 896 432 747 268 838
Fragments arranged by size:
896 838 747 432 348 336 268 252
248 210 174 164 88 88 84 80
80 21 4 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1221 1221
Size: 5366
EcoO109I rG'GnC_Cy
Cuts at: 637 1061 1310 2505 637
Size: 424 249 1195 3498
Fragments arranged by size:
3498 1195 424 249
EcoRI G'AATT_C
Cuts at: 741 917 1001 1085 1589 1757 1925 2093 2261
Size: 176 84 84 504 168 168 168 168
Cuts at: 2261 2370 741
Size: 109 3737
Fragments arranged by size:
3737 504 176 168 168 168 168 109
84 84
EcoRV GAT'ATC
Cuts at: 2317 2317
Size: 5366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3385 3385
Size: 5366
FspI TGC'GCA
Cuts at: 3147 3147
Size: 5366
HaeII r_GCGC'y
Cuts at: 2800 3048 4070 5049 2800
Size: 248 1022 979 3117
Fragments arranged by size:
3117 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 964 996 1050 1082 2058 2090 2142
Size: 32 826 32 54 32 976 32 52
Cuts at: 2142 2154 2174 2186 2226 2258 2334 2366 3208
Size: 12 20 12 40 32 76 32 842
Cuts at: 3208 3238 3240 3270 106
Size: 30 2 30 2202
Fragments arranged by size:
2202 976 842 826 76 54 52 40
32 32 32 32 32 32 30 30
20 12 12 2
HincII GTy'rAC
Cuts at: 2405 2405
Size: 5366
HindIII A'AGCT_T
Cuts at: 1691 1718 1691
Size: 27 5339
KasI G'GCGC_C
Cuts at: 2796 3044 2796
Size: 248 5118
KpnI G_GTAC'C
Cuts at: 398 2386 398
Size: 1988 3378
MluI A'CGCG_T
Cuts at: 4799 4799
Size: 5366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 545 4037 4221 545
Size: 3492 184 1690
Fragments arranged by size:
3492 1690 184
MscI TGG'CCA
Cuts at: 251 3127 251
Size: 2876 2490
MslI CAynn'nnrTG
Cuts at: 942 1026 1346 1793 3355 3482 942
Size: 84 320 447 1562 127 2826
Fragments arranged by size:
2826 1562 447 320 127 84
MspA1I CmG'CkG
Cuts at: 240 2477 2791 3151 4164 4409 240
Size: 2237 314 360 1013 245 1197
Fragments arranged by size:
2237 1197 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3547 3547
Size: 5366
NarI GG'CG_CC
Cuts at: 2797 3045 2797
Size: 248 5118
NcoI C'CATG_G
Cuts at: 107 364 107
Size: 257 5109
NdeI CA'TA_TG
Cuts at: 1334 1334
Size: 5366
NgoMIV G'CCGG_C
Cuts at: 3545 3545
Size: 5366
NspI r_CATG'y
Cuts at: 2419 2919 3826 2419
Size: 500 907 3959
Fragments arranged by size:
3959 907 500
PciI A'CATG_T
Cuts at: 3822 3822
Size: 5366
PflMI CCAn_nnn'nTGG
Cuts at: 1155 1155
Size: 5366
PmeI GTTT'AAAC
Cuts at: 2365 2365
Size: 5366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 4505
Size: 32 5334
PpuMI rG'GwC_Cy
Cuts at: 1061 1061
Size: 5366
PsiI TTA'TAA
Cuts at: 904 2201 904
Size: 1297 4069
PspOMI G'GGCC_C
Cuts at: 1309 1309
Size: 5366
PstI C_TGCA'G
Cuts at: 2413 3098 2413
Size: 685 4681
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5366
PvuII CAG'CTG
Cuts at: 240 2791 3151 240
Size: 2551 360 2455
Fragments arranged by size:
2551 2455 360
RsrII CG'GwC_CG
Cuts at: 3561 3561
Size: 5366
SacI G_AGCT'C
Cuts at: 2071 2380 2071
Size: 309 5057
SalI G'TCGA_C
Cuts at: 2403 2403
Size: 5366
SapI GCTCTTCn'nnn_
Cuts at: 458 3389 3599 458
Size: 2931 210 2225
Fragments arranged by size:
2931 2225 210
SbfI CC_TGCA'GG
Cuts at: 2413 2413
Size: 5366
ScaI AGT'ACT
Cuts at: 4883 4883
Size: 5366
SexAI A'CCwGG_T
Cuts at: 296 296
Size: 5366
SfcI C'TryA_G
Cuts at: 33 305 2409 3094 4087 4278 33
Size: 272 2104 685 993 191 1121
Fragments arranged by size:
2104 1121 993 685 272 191
SfoI GGC'GCC
Cuts at: 2798 3046 2798
Size: 248 5118
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5366
SmaI CCC'GGG
Cuts at: 2388 2388
Size: 5366
SmlI C'TyrA_G
Cuts at: 1682 2521 3436 3928 4190 1682
Size: 839 915 492 262 2858
Fragments arranged by size:
2858 915 839 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5366
SphI G_CATG'C
Cuts at: 2419 2919 2419
Size: 500 4866
StuI AGG'CCT
Cuts at: 1300 1552 1300
Size: 252 5114
StyI C'CwwG_G
Cuts at: 107 144 364 2500 2854 107
Size: 37 220 2136 354 2619
Fragments arranged by size:
2619 2136 354 220 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2949 3082 3541 3750 2949
Size: 133 459 209 4565
Fragments arranged by size:
4565 459 209 133
TatI w'GTAC_w
Cuts at: 4881 4881
Size: 5366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4883 4915 4883
Size: 32 5334
Tth111I GACn'n_nGTC
Cuts at: 3163 3163
Size: 5366
XbaI T'CTAG_A
Cuts at: 2397 2397
Size: 5366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2861 2861
Size: 5366
XmaI C'CCGG_G
Cuts at: 2386 2386
Size: 5366
XmnI GAAnn'nnTTC
Cuts at: 1384 1757 1804 2265 1384
Size: 373 47 461 4485
Fragments arranged by size:
4485 461 373 47
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BseRI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI DraIII FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI