Restriction map for
pF1KB6235
(Circular) (Six-base) MAPSORT of: pF1KB6235.seq Check: 9372 from: 1 to: 5360
pF1KB6235 5360 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 183 183
Size: 5360
AatII G_ACGT'C
Cuts at: 1448 1448
Size: 5360
AccI GT'mk_AC
Cuts at: 1332 1694 1989 2398 1332
Size: 362 295 409 4294
Fragments arranged by size:
4294 409 362 295
Acc65I G'GTAC_C
Cuts at: 2376 2376
Size: 5360
AclI AA'CG_TT
Cuts at: 5200 5200
Size: 5360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 1771 2153 3185 3617 4364 4632 1630
Size: 141 382 1032 432 747 268 2358
Fragments arranged by size:
2358 1032 747 432 382 268 141
AfeI AGC'GCT
Cuts at: 2328 2328
Size: 5360
AflIII A'CryG_T
Cuts at: 258 1604 2712 3816 4793 258
Size: 1346 1108 1104 977 825
Fragments arranged by size:
1346 1108 1104 977 825
AgeI A'CCGG_T
Cuts at: 3748 3748
Size: 5360
AhdI GACnn_n'nnGTC
Cuts at: 621 621
Size: 5360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1832 1832
Size: 5360
AlwNI CAG_nnn'CTG
Cuts at: 124 666 1593 1899 2665 4232 124
Size: 542 927 306 766 1567 1252
Fragments arranged by size:
1567 1252 927 766 542 306
ApaLI G'TGCA_C
Cuts at: 4130 4130
Size: 5360
ApoI r'AATT_y
Cuts at: 534 1488 2364 4713 4969 534
Size: 954 876 2349 256 925
Fragments arranged by size:
2349 954 925 876 256
AseI AT'TA_AT
Cuts at: 20 790 20
Size: 770 4590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
AvaI C'yCGr_G
Cuts at: 563 1088 2242 2380 563
Size: 525 1154 138 3543
Fragments arranged by size:
3543 1154 525 138
BamHI G'GATC_C
Cuts at: 2385 2385
Size: 5360
BanI G'GyrC_C
Cuts at: 2376 2790 3038 3073 2376
Size: 414 248 35 4663
Fragments arranged by size:
4663 414 248 35
BanII G_rGCy'C
Cuts at: 2374 3404 2374
Size: 1030 4330
BbeI G_GCGC'C
Cuts at: 2794 3042 2794
Size: 248 5112
BbsI GAAGACnn'nnnn_
Cuts at: 914 1872 914
Size: 958 4402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5326
BciVI GTATCCnnnnn_n'
Cuts at: 347 3258 4019 5360 347
Size: 2911 761 1341 347
Fragments arranged by size:
2911 1341 761 347
BclI T'GATC_A
Cuts at: 2880 2880
Size: 5360
BfrBI ATG'CAT
Cuts at: 1084 1084
Size: 5360
BglII A'GATC_T
Cuts at: 2875 4912 2875
Size: 2037 3323
BlpI GC'TnA_GC
Cuts at: 2203 2472 2203
Size: 269 5091
Bme1580I G_kGCm'C
Cuts at: 451 835 2985 3078 4134 451
Size: 384 2150 93 1056 1677
Fragments arranged by size:
2150 1677 1056 384 93
BmgBI CAC'GTC
Cuts at: 1607 1607
Size: 5360
BmrI ACTGGGnnnn_n'
Cuts at: 134 757 1865 2572 2653 2988 5132 134
Size: 623 1108 707 81 335 2144 362
Fragments arranged by size:
2144 1108 707 623 362 335 81
BmtI G_CTAG'C
Cuts at: 1964 1964
Size: 5360
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 502 3727 502
Size: 3225 2135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 521 643 1825 395
Size: 126 122 1182 3930
Fragments arranged by size:
3930 1182 126 122
Bpu10I CC'TnA_GC
Cuts at: 272 2036 272
Size: 1764 3596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 2162 2536 3415 3907 4205 380
Size: 1782 374 879 492 298 1535
Fragments arranged by size:
1782 1535 879 492 374 298
BsaI GGTCTCn'nnnn_
Cuts at: 432 5064 432
Size: 4632 728
BsaAI yAC'GTr
Cuts at: 2713 2713
Size: 5360
BsaBI GATnn'nnATC
Cuts at: 6 1435 2874 4911 6
Size: 1429 1439 2037 455
Fragments arranged by size:
2037 1439 1429 455
BsaHI Gr'CG_yC
Cuts at: 470 1445 2791 3039 3741 5237 470
Size: 975 1346 248 702 1496 593
Fragments arranged by size:
1496 1346 975 702 593 248
BsaWI w'CCGG_w
Cuts at: 2549 3070 3748 4022 4169 4580 2549
Size: 521 678 274 147 411 3329
Fragments arranged by size:
3329 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2249 2279 2249
Size: 30 5330
BseYI C'CCAG_C
Cuts at: 573 2785 3543 4120 4616 573
Size: 2212 758 577 496 1317
Fragments arranged by size:
2212 1317 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 298 1658 2343 265
Size: 33 1360 685 3282
Fragments arranged by size:
3282 1360 685 33
BsiEI CG_ry'CG
Cuts at: 104 2902 2948 4156 104
Size: 2798 46 1208 1308
Fragments arranged by size:
2798 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1095 1187 1801 2291 2374 3152 3342 4134 1095
Size: 92 614 490 83 778 190 792 2321
Fragments arranged by size:
2321 792 778 614 490 190 92 83
BsmBI CGTCTCn'nnnn_
Cuts at: 592 592
Size: 5360
Bsp1286I G_dGCh'C
Cuts at: 451 835 1095 1187 1801 2291 2374 2985 3078
Size: 384 260 92 614 490 83 611 93
Cuts at: 3078 3152 3342 3404 4134 451
Size: 74 190 62 730 1677
Fragments arranged by size:
1677 730 614 611 490 384 260 190
93 92 83 74 62
BspEI T'CCGG_A
Cuts at: 4580 4580
Size: 5360
BspHI T'CATG_A
Cuts at: 4536 4536
Size: 5360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 183 222 240 2410 2624 2926 3307 183
Size: 39 18 2170 214 302 381 2236
Fragments arranged by size:
2236 2170 381 302 214 39 18
BsrBI CCG'CTC
Cuts at: 50 271 3652 3706 5190 5355 50
Size: 221 3381 54 1484 165 55
Fragments arranged by size:
3381 1484 221 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3272 3272
Size: 5360
BsrFI r'CCGG_y
Cuts at: 3358 3539 3748 3358
Size: 181 209 4970
Fragments arranged by size:
4970 209 181
BsrGI T'GTAC_A
Cuts at: 359 719 359
Size: 360 5000
BssSI C'ACGA_G
Cuts at: 1634 3631 3989 1634
Size: 1997 358 3005
Fragments arranged by size:
3005 1997 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1636 1899 2737 1636
Size: 263 838 4259
Fragments arranged by size:
4259 838 263
BstBI TT'CG_AA
Cuts at: 1485 3721 1485
Size: 2236 3124
BstXI CCAn_nnnn'nTGG
Cuts at: 373 828 862 373
Size: 455 34 4871
Fragments arranged by size:
4871 455 34
BstYI r'GATC_y
Cuts at: 1 2385 2852 2875 3210 3456 4468 4554 4566
Size: 2384 467 23 335 246 1012 86 12
Cuts at: 4566 4912 1
Size: 346 449
Fragments arranged by size:
2384 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 422 1404 422
Size: 982 4378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3310 3491 3654 3310
Size: 181 163 5016
Fragments arranged by size:
5016 181 163
BtsI GCAGTG_nn'
Cuts at: 188 1162 1646 1816 1959 2133 2345 2603 188
Size: 974 484 170 143 174 212 258 2945
Fragments arranged by size:
2945 974 484 258 212 174 170 143
DraI TTT'AAA
Cuts at: 2087 2359 2087
Size: 272 5088
DraIII CAC_nnn'GTG
Cuts at: 549 549
Size: 5360
DrdI GACnn_nn'nnGTC
Cuts at: 3066 3924 3066
Size: 858 4502
EaeI y'GGCC_r
Cuts at: 2945 3119 3510 3537 4882 2945
Size: 174 391 27 1345 3423
Fragments arranged by size:
3423 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2945 2945
Size: 5360
EarI CTCTTCn'nnn_
Cuts at: 647 1128 1790 2172 3383 3593 647
Size: 481 662 382 1211 210 2414
Fragments arranged by size:
2414 1211 662 481 382 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 396 3568 3878 4024 5170 396
Size: 3172 310 146 1146 586
Fragments arranged by size:
3172 1146 586 310 146
EcoICRI GAG'CTC
Cuts at: 2372 2372
Size: 5360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 521 643 1630 1771 1825 2153 3185 3617
Size: 126 122 987 141 54 328 1032 432
Cuts at: 3617 4364 4632 395
Size: 747 268 1123
Fragments arranged by size:
1123 1032 987 747 432 328 268 141
126 122 54
EcoNI CCTnn'n_nnAGG
Cuts at: 1624 1624
Size: 5360
EcoO109I rG'GnC_Cy
Cuts at: 2499 2499
Size: 5360
EcoRI G'AATT_C
Cuts at: 534 2364 534
Size: 1830 3530
EcoRV GAT'ATC
Cuts at: 1155 1155
Size: 5360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1531 3379 1531
Size: 1848 3512
FspI TGC'GCA
Cuts at: 3141 3141
Size: 5360
HaeII r_GCGC'y
Cuts at: 1263 2330 2794 3042 4064 5043 1263
Size: 1067 464 248 1022 979 1580
Fragments arranged by size:
1580 1067 1022 979 464 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 124 144 156 473 505 607 639 1144
Size: 12 20 12 317 32 102 32 505
Cuts at: 1144 1176 3202 3232 3234 3264 112
Size: 32 2026 30 2 30 2208
Fragments arranged by size:
2208 2026 505 317 102 32 32 32
30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 1015 1333 1695 2399 1015
Size: 318 362 704 3976
Fragments arranged by size:
3976 704 362 318
KasI G'GCGC_C
Cuts at: 2790 3038 2790
Size: 248 5112
KpnI G_GTAC'C
Cuts at: 2380 2380
Size: 5360
MfeI C'AATT_G
Cuts at: 581 581
Size: 5360
MluI A'CGCG_T
Cuts at: 4793 4793
Size: 5360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 650 1303 4031 4215 110
Size: 540 653 2728 184 1255
Fragments arranged by size:
2728 1255 653 540 184
MscI TGG'CCA
Cuts at: 3121 3121
Size: 5360
MslI CAynn'nnrTG
Cuts at: 860 3349 3476 860
Size: 2489 127 2744
Fragments arranged by size:
2744 2489 127
MspA1I CmG'CkG
Cuts at: 1570 2471 2785 3145 4158 4403 1570
Size: 901 314 360 1013 245 2527
Fragments arranged by size:
2527 1013 901 360 314 245
NaeI GCC'GGC
Cuts at: 3541 3541
Size: 5360
NarI GG'CG_CC
Cuts at: 2791 3039 2791
Size: 248 5112
NcoI C'CATG_G
Cuts at: 1404 1404
Size: 5360
NgoMIV G'CCGG_C
Cuts at: 3539 3539
Size: 5360
NheI G'CTAG_C
Cuts at: 1960 1960
Size: 5360
NsiI A_TGCA'T
Cuts at: 1086 1086
Size: 5360
NspI r_CATG'y
Cuts at: 262 915 2232 2413 2913 3820 262
Size: 653 1317 181 500 907 1802
Fragments arranged by size:
1802 1317 907 653 500 181
PciI A'CATG_T
Cuts at: 258 3816 258
Size: 3558 1802
PflMI CCAn_nnn'nTGG
Cuts at: 441 1105 1285 1422 441
Size: 664 180 137 4379
Fragments arranged by size:
4379 664 180 137
PfoI T'CCnGG_A
Cuts at: 1709 2100 1709
Size: 391 4969
PmeI GTTT'AAAC
Cuts at: 2359 2359
Size: 5360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 4499
Size: 32 5328
PstI C_TGCA'G
Cuts at: 1169 2352 2407 3092 1169
Size: 1183 55 685 3437
Fragments arranged by size:
3437 1183 685 55
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5360
PvuII CAG'CTG
Cuts at: 2785 3145 2785
Size: 360 5000
RsrII CG'GwC_CG
Cuts at: 3555 3555
Size: 5360
SacI G_AGCT'C
Cuts at: 2374 2374
Size: 5360
SalI G'TCGA_C
Cuts at: 1331 1693 2397 1331
Size: 362 704 4294
Fragments arranged by size:
4294 704 362
SapI GCTCTTCn'nnn_
Cuts at: 3383 3593 3383
Size: 210 5150
SbfI CC_TGCA'GG
Cuts at: 2407 2407
Size: 5360
ScaI AGT'ACT
Cuts at: 2304 4877 2304
Size: 2573 2787
SfcI C'TryA_G
Cuts at: 33 1165 1917 2057 2330 2348 2403 3088 4081
Size: 1132 752 140 273 18 55 685 993
Cuts at: 4081 4272 33
Size: 191 1121
Fragments arranged by size:
1132 1121 993 752 685 273 191 140
55 18
SfoI GGC'GCC
Cuts at: 2792 3040 2792
Size: 248 5112
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
SmaI CCC'GGG
Cuts at: 2382 2382
Size: 5360
SmlI C'TyrA_G
Cuts at: 395 1088 2177 2242 2515 3430 3922 4184 395
Size: 693 1089 65 273 915 492 262 1571
Fragments arranged by size:
1571 1089 915 693 492 273 262 65
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5360
SphI G_CATG'C
Cuts at: 2232 2413 2913 2232
Size: 181 500 4679
Fragments arranged by size:
4679 500 181
SspI AAT'ATT
Cuts at: 1199 1536 1199
Size: 337 5023
StyI C'CwwG_G
Cuts at: 1404 1559 2494 2848 1404
Size: 155 935 354 3916
Fragments arranged by size:
3916 935 354 155
SwaI ATTT'AAAT
Cuts at: 2087 2087
Size: 5360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1716 2943 3076 3535 3744 1716
Size: 1227 133 459 209 3332
Fragments arranged by size:
3332 1227 459 209 133
TatI w'GTAC_w
Cuts at: 213 359 719 2302 4875 213
Size: 146 360 1583 2573 698
Fragments arranged by size:
2573 1583 698 360 146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4877 4909 4877
Size: 32 5328
Tth111I GACn'n_nGTC
Cuts at: 126 740 763 3157 126
Size: 614 23 2394 2329
Fragments arranged by size:
2394 2329 614 23
XbaI T'CTAG_A
Cuts at: 2391 2391
Size: 5360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2855 2855
Size: 5360
XhoI C'TCGA_G
Cuts at: 1088 2242 1088
Size: 1154 4206
XmaI C'CCGG_G
Cuts at: 2380 2380
Size: 5360
ZraI GAC'GTC
Cuts at: 1446 1446
Size: 5360
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AflII AleI AloI ApaI AscI AvrII BaeI BbvCI
BglI BseRI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI FseI FspAI HindIII HpaI NdeI NotI
NruI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI XmnI