Restriction map for
pF1KB6234
(Circular) (Six-base) MAPSORT of: pF1KB6234.seq Check: 1616 from: 1 to: 5144
pF1KB6234 5144 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 878 878
Size: 5144
AccI GT'mk_AC
Cuts at: 2182 2182
Size: 5144
Acc65I G'GTAC_C
Cuts at: 2160 2160
Size: 5144
AclI AA'CG_TT
Cuts at: 4984 4984
Size: 5144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 918 1426 1478 2969 3401 4148 4416 776
Size: 142 508 52 1491 432 747 268 1504
Fragments arranged by size:
1504 1491 747 508 432 268 142 52
AfeI AGC'GCT
Cuts at: 1518 1518
Size: 5144
AflIII A'CryG_T
Cuts at: 491 1158 2496 3600 4577 491
Size: 667 1338 1104 977 1058
Fragments arranged by size:
1338 1104 1058 977 667
AgeI A'CCGG_T
Cuts at: 3532 3532
Size: 5144
AleI CACnn'nnGTG
Cuts at: 322 925 322
Size: 603 4541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1288 1288
Size: 5144
AlwNI CAG_nnn'CTG
Cuts at: 1883 2449 4016 1883
Size: 566 1567 3011
Fragments arranged by size:
3011 1567 566
ApaI G_GGCC'C
Cuts at: 728 1284 728
Size: 556 4588
ApaLI G'TGCA_C
Cuts at: 460 802 3914 460
Size: 342 3112 1690
Fragments arranged by size:
3112 1690 342
ApoI r'AATT_y
Cuts at: 1492 2148 4497 4753 1492
Size: 656 2349 256 1883
Fragments arranged by size:
2349 1883 656 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5144
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
AvaI C'yCGr_G
Cuts at: 1050 2164 1050
Size: 1114 4030
BamHI G'GATC_C
Cuts at: 2169 2169
Size: 5144
BanI G'GyrC_C
Cuts at: 163 1600 2080 2160 2574 2822 2857 163
Size: 1437 480 80 414 248 35 2450
Fragments arranged by size:
2450 1437 480 414 248 80 35
BanII G_rGCy'C
Cuts at: 728 790 1284 1445 2158 3188 728
Size: 62 494 161 713 1030 2684
Fragments arranged by size:
2684 1030 713 494 161 62
BbeI G_GCGC'C
Cuts at: 1604 2578 2826 1604
Size: 974 248 3922
Fragments arranged by size:
3922 974 248
BbsI GAAGACnn'nnnn_
Cuts at: 1955 1955
Size: 5144
BbvCI CC'TCA_GC
Cuts at: 569 569
Size: 5144
BciVI GTATCCnnnnn_n'
Cuts at: 3042 3803 5144 3042
Size: 761 1341 3042
Fragments arranged by size:
3042 1341 761
BclI T'GATC_A
Cuts at: 428 2664 428
Size: 2236 2908
BfrBI ATG'CAT
Cuts at: 1528 1528
Size: 5144
BglI GCCn_nnn'nGGC
Cuts at: 543 944 1219 543
Size: 401 275 4468
Fragments arranged by size:
4468 401 275
BglII A'GATC_T
Cuts at: 2659 4696 2659
Size: 2037 3107
BlpI GC'TnA_GC
Cuts at: 2256 2256
Size: 5144
Bme1580I G_kGCm'C
Cuts at: 168 464 728 806 1284 2769 2862 3918 168
Size: 296 264 78 478 1485 93 1056 1394
Fragments arranged by size:
1485 1394 1056 478 296 264 93 78
BmrI ACTGGGnnnn_n'
Cuts at: 843 1600 2356 2437 2772 4916 843
Size: 757 756 81 335 2144 1071
Fragments arranged by size:
2144 1071 757 756 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1301 2124 3511 1301
Size: 823 1387 2934
Fragments arranged by size:
2934 1387 823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 579 1213 1486 286
Size: 293 634 273 3944
Fragments arranged by size:
3944 634 293 273
Bpu10I CC'TnA_GC
Cuts at: 169 569 2068 169
Size: 400 1499 3245
Fragments arranged by size:
3245 1499 400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 1429 2320 3199 3691 3989 1360
Size: 69 891 879 492 298 2515
Fragments arranged by size:
2515 891 879 492 298 69
BsaI GGTCTCn'nnnn_
Cuts at: 125 475 494 1804 4848 125
Size: 350 19 1310 3044 421
Fragments arranged by size:
3044 1310 421 350 19
BsaAI yAC'GTr
Cuts at: 858 2497 858
Size: 1639 3505
BsaBI GATnn'nnATC
Cuts at: 6 2658 4695 6
Size: 2652 2037 455
Fragments arranged by size:
2652 2037 455
BsaHI Gr'CG_yC
Cuts at: 1601 2575 2823 3525 5021 1601
Size: 974 248 702 1496 1724
Fragments arranged by size:
1724 1496 974 702 248
BsaWI w'CCGG_w
Cuts at: 834 1497 2054 2333 2854 3532 3806 3953 4364
Size: 663 557 279 521 678 274 147 411
Cuts at: 4364 834
Size: 1614
Fragments arranged by size:
1614 678 663 557 521 411 279 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 655 685 1771 1801 655
Size: 30 1086 30 3998
Fragments arranged by size:
3998 1086 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1038 1591 1038
Size: 553 4591
BseYI C'CCAG_C
Cuts at: 216 412 1876 2134 2569 3327 3904 4400 216
Size: 196 1464 258 435 758 577 496 960
Fragments arranged by size:
1464 960 758 577 496 435 258 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 863 1099 1294 2143 322
Size: 541 236 195 849 3323
Fragments arranged by size:
3323 849 541 236 195
BsiEI CG_ry'CG
Cuts at: 104 1015 2686 2732 3940 104
Size: 911 1671 46 1208 1308
Fragments arranged by size:
1671 1308 1208 911 46
BsiHKAI G_wGCw'C
Cuts at: 464 806 1445 1835 2158 2936 3126 3918 464
Size: 342 639 390 323 778 190 792 1690
Fragments arranged by size:
1690 792 778 639 390 342 323 190
BsmI GAATG_Cn'
Cuts at: 2019 2019
Size: 5144
Bsp1286I G_dGCh'C
Cuts at: 168 464 728 790 806 1284 1445 1835 2158
Size: 296 264 62 16 478 161 390 323
Cuts at: 2158 2769 2862 2936 3126 3188 3918 168
Size: 611 93 74 190 62 730 1394
Fragments arranged by size:
1394 730 611 478 390 323 296 264
190 161 93 74 62 62 16
BspEI T'CCGG_A
Cuts at: 834 1497 4364 834
Size: 663 2867 1614
Fragments arranged by size:
2867 1614 663
BspHI T'CATG_A
Cuts at: 4320 4320
Size: 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 853 878 2194 2408 2710 3091 853
Size: 25 1316 214 302 381 2906
Fragments arranged by size:
2906 1316 381 302 214 25
BsrBI CCG'CTC
Cuts at: 50 159 180 3436 3490 4974 5139 50
Size: 109 21 3256 54 1484 165 55
Fragments arranged by size:
3256 1484 165 109 55 54 21
BsrDI GCAATG_nn'
Cuts at: 1134 3056 1134
Size: 1922 3222
BsrFI r'CCGG_y
Cuts at: 818 2129 3142 3323 3532 818
Size: 1311 1013 181 209 2430
Fragments arranged by size:
2430 1311 1013 209 181
BssSI C'ACGA_G
Cuts at: 1623 3415 3773 1623
Size: 1792 358 2994
Fragments arranged by size:
2994 1792 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2521 2521
Size: 5144
BstBI TT'CG_AA
Cuts at: 3505 3505
Size: 5144
BstEII G'GTnAC_C
Cuts at: 865 1345 865
Size: 480 4664
BstXI CCAn_nnnn'nTGG
Cuts at: 496 1821 496
Size: 1325 3819
BstYI r'GATC_y
Cuts at: 1 2169 2636 2659 2994 3240 4252 4338 4350
Size: 2168 467 23 335 246 1012 86 12
Cuts at: 4350 4696 1
Size: 346 449
Fragments arranged by size:
2168 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1363 1363
Size: 5144
BtgI C'CryG_G
Cuts at: 107 1718 107
Size: 1611 3533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 510 3094 3275 3438 510
Size: 2584 181 163 2216
Fragments arranged by size:
2584 2216 181 163
BtsI GCAGTG_nn'
Cuts at: 1243 1704 2079 2131 2387 1243
Size: 461 375 52 256 4000
Fragments arranged by size:
4000 461 375 256 52
ClaI AT'CG_AT
Cuts at: 1907 1907
Size: 5144
DraI TTT'AAA
Cuts at: 2143 2143
Size: 5144
DrdI GACnn_nn'nnGTC
Cuts at: 2850 3708 2850
Size: 858 4286
EaeI y'GGCC_r
Cuts at: 651 1012 1701 1892 2729 2903 3294 3321 4666
Size: 361 689 191 837 174 391 27 1345
Cuts at: 4666 651
Size: 1129
Fragments arranged by size:
1345 1129 837 689 391 361 191 174
27
EagI C'GGCC_G
Cuts at: 1012 2729 1012
Size: 1717 3427
EarI CTCTTCn'nnn_
Cuts at: 1561 3167 3377 1561
Size: 1606 210 3328
Fragments arranged by size:
3328 1606 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 574 1600 3352 3662 3808 4954 574
Size: 1026 1752 310 146 1146 764
Fragments arranged by size:
1752 1146 1026 764 310 146
EcoICRI GAG'CTC
Cuts at: 1443 2156 1443
Size: 713 4431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 579 776 918 1213 1426 1478 1486 2969
Size: 293 197 142 295 213 52 8 1483
Cuts at: 2969 3401 4148 4416 286
Size: 432 747 268 1014
Fragments arranged by size:
1483 1014 747 432 295 293 268 213
197 142 52 8
EcoNI CCTnn'n_nnAGG
Cuts at: 732 732
Size: 5144
EcoO109I rG'GnC_Cy
Cuts at: 330 725 1280 1281 1588 2087 2283 330
Size: 395 555 1 307 499 196 3191
Fragments arranged by size:
3191 555 499 395 307 196 1
EcoRI G'AATT_C
Cuts at: 2148 2148
Size: 5144
EcoRV GAT'ATC
Cuts at: 1725 1725
Size: 5144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1431 3163 1431
Size: 1732 3412
FspI TGC'GCA
Cuts at: 2925 2925
Size: 5144
HaeII r_GCGC'y
Cuts at: 186 538 1520 1604 2578 2826 3848 4827 186
Size: 352 982 84 974 248 1022 979 503
Fragments arranged by size:
1022 982 979 974 503 352 248 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 122 154 1627 1659 1771 1803 2986 3016 3018
Size: 32 1473 32 112 32 1183 30 2
Cuts at: 3018 3048 122
Size: 30 2218
Fragments arranged by size:
2218 1473 1183 112 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 120 258 684 963 1857 2183 120
Size: 138 426 279 894 326 3081
Fragments arranged by size:
3081 894 426 326 279 138
HindIII A'AGCT_T
Cuts at: 880 880
Size: 5144
KasI G'GCGC_C
Cuts at: 1600 2574 2822 1600
Size: 974 248 3922
Fragments arranged by size:
3922 974 248
KpnI G_GTAC'C
Cuts at: 2164 2164
Size: 5144
MluI A'CGCG_T
Cuts at: 4577 4577
Size: 5144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1118 2006 3815 3999 1118
Size: 888 1809 184 2263
Fragments arranged by size:
2263 1809 888 184
MscI TGG'CCA
Cuts at: 653 1894 2905 653
Size: 1241 1011 2892
Fragments arranged by size:
2892 1241 1011
MslI CAynn'nnrTG
Cuts at: 322 490 751 925 1127 1355 3133 3260 322
Size: 168 261 174 202 228 1778 127 2206
Fragments arranged by size:
2206 1778 261 228 202 174 168 127
MspA1I CmG'CkG
Cuts at: 336 657 722 796 1011 1430 1549 2004 2255
Size: 321 65 74 215 419 119 455 251
Cuts at: 2255 2569 2929 3942 4187 336
Size: 314 360 1013 245 1293
Fragments arranged by size:
1293 1013 455 419 360 321 314 251
245 215 119 74 65
NaeI GCC'GGC
Cuts at: 2131 3325 2131
Size: 1194 3950
NarI GG'CG_CC
Cuts at: 1601 2575 2823 1601
Size: 974 248 3922
Fragments arranged by size:
3922 974 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5144
NgoMIV G'CCGG_C
Cuts at: 2129 3323 2129
Size: 1194 3950
NsiI A_TGCA'T
Cuts at: 1530 1530
Size: 5144
NspI r_CATG'y
Cuts at: 460 495 1162 2197 2697 3604 460
Size: 35 667 1035 500 907 2000
Fragments arranged by size:
2000 1035 907 667 500 35
PciI A'CATG_T
Cuts at: 491 1158 3600 491
Size: 667 2442 2035
Fragments arranged by size:
2442 2035 667
PflMI CCAn_nnn'nTGG
Cuts at: 660 660
Size: 5144
PmeI GTTT'AAAC
Cuts at: 2143 2143
Size: 5144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 5112
PpuMI rG'GwC_Cy
Cuts at: 330 1588 330
Size: 1258 3886
PspOMI G'GGCC_C
Cuts at: 724 1280 724
Size: 556 4588
PstI C_TGCA'G
Cuts at: 239 1868 2191 2876 239
Size: 1629 323 685 2507
Fragments arranged by size:
2507 1629 685 323
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5144
PvuII CAG'CTG
Cuts at: 657 1549 2569 2929 657
Size: 892 1020 360 2872
Fragments arranged by size:
2872 1020 892 360
RsrII CG'GwC_CG
Cuts at: 3339 3339
Size: 5144
SacI G_AGCT'C
Cuts at: 1445 2158 1445
Size: 713 4431
SalI G'TCGA_C
Cuts at: 2181 2181
Size: 5144
SanDI GG'GwC_CC
Cuts at: 330 330
Size: 5144
SapI GCTCTTCn'nnn_
Cuts at: 3167 3377 3167
Size: 210 4934
SbfI CC_TGCA'GG
Cuts at: 2191 2191
Size: 5144
ScaI AGT'ACT
Cuts at: 646 4661 646
Size: 4015 1129
SexAI A'CCwGG_T
Cuts at: 1590 1820 1590
Size: 230 4914
SfcI C'TryA_G
Cuts at: 33 235 1864 2187 2872 3865 4056 33
Size: 202 1629 323 685 993 191 1121
Fragments arranged by size:
1629 1121 993 685 323 202 191
SfoI GGC'GCC
Cuts at: 1602 2576 2824 1602
Size: 974 248 3922
Fragments arranged by size:
3922 974 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
SmaI CCC'GGG
Cuts at: 2166 2166
Size: 5144
SmlI C'TyrA_G
Cuts at: 1375 1444 2299 3214 3706 3968 1375
Size: 69 855 915 492 262 2551
Fragments arranged by size:
2551 915 855 492 262 69
SnaBI TAC'GTA
Cuts at: 858 858
Size: 5144
SpeI A'CTAG_T
Cuts at: 67 957 67
Size: 890 4254
SphI G_CATG'C
Cuts at: 2197 2697 2197
Size: 500 4644
StyI C'CwwG_G
Cuts at: 107 1836 2278 2632 107
Size: 1729 442 354 2619
Fragments arranged by size:
2619 1729 442 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2727 2860 3319 3528 2727
Size: 133 459 209 4343
Fragments arranged by size:
4343 459 209 133
TatI w'GTAC_w
Cuts at: 644 4659 644
Size: 4015 1129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 655 687 4661 4693 655
Size: 32 3974 32 1106
Fragments arranged by size:
3974 1106 32 32
Tth111I GACn'n_nGTC
Cuts at: 2941 2941
Size: 5144
XbaI T'CTAG_A
Cuts at: 2175 2175
Size: 5144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2639 2639
Size: 5144
XmaI C'CCGG_G
Cuts at: 2164 2164
Size: 5144
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BcgI
BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I CspCI
DraIII FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI