Restriction map for
pF1KB6225
(Circular) (Six-base) MAPSORT of: pF1KB6225.seq Check: 8228 from: 1 to: 5126
pF1KB6225 5126 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1629 1629
Size: 5126
AccI GT'mk_AC
Cuts at: 806 1178 1328 2164 806
Size: 372 150 836 3768
Fragments arranged by size:
3768 836 372 150
Acc65I G'GTAC_C
Cuts at: 545 2142 545
Size: 1597 3529
AclI AA'CG_TT
Cuts at: 2092 4966 2092
Size: 2874 2252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 2951 3383 4130 4398 731
Size: 2220 432 747 268 1459
Fragments arranged by size:
2220 1459 747 432 268
AfeI AGC'GCT
Cuts at: 457 457
Size: 5126
AflIII A'CryG_T
Cuts at: 2478 3582 4559 2478
Size: 1104 977 3045
Fragments arranged by size:
3045 1104 977
AgeI A'CCGG_T
Cuts at: 3514 3514
Size: 5126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 671 671
Size: 5126
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1643 1675 1643
Size: 32 5094
AlwNI CAG_nnn'CTG
Cuts at: 358 542 843 1467 2431 3998 358
Size: 184 301 624 964 1567 1486
Fragments arranged by size:
1567 1486 964 624 301 184
ApaI G_GGCC'C
Cuts at: 118 485 1035 118
Size: 367 550 4209
Fragments arranged by size:
4209 550 367
ApaLI G'TGCA_C
Cuts at: 3896 3896
Size: 5126
ApoI r'AATT_y
Cuts at: 2083 2130 4479 4735 2083
Size: 47 2349 256 2474
Fragments arranged by size:
2474 2349 256 47
AseI AT'TA_AT
Cuts at: 20 20
Size: 5126
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5126
AvaI C'yCGr_G
Cuts at: 197 1193 1886 2146 197
Size: 996 693 260 3177
Fragments arranged by size:
3177 996 693 260
AvrII C'CTAG_G
Cuts at: 1158 1158
Size: 5126
BamHI G'GATC_C
Cuts at: 2151 2151
Size: 5126
BanI G'GyrC_C
Cuts at: 210 545 1020 1607 1858 2142 2556 2804 2839
Size: 335 475 587 251 284 414 248 35
Cuts at: 2839 210
Size: 2497
Fragments arranged by size:
2497 587 475 414 335 284 251 248
35
BanII G_rGCy'C
Cuts at: 118 143 485 645 1015 1035 1078 2140 3170
Size: 25 342 160 370 20 43 1062 1030
Cuts at: 3170 118
Size: 2074
Fragments arranged by size:
2074 1062 1030 370 342 160 43 25
20
BbeI G_GCGC'C
Cuts at: 1024 1611 2560 2808 1024
Size: 587 949 248 3342
Fragments arranged by size:
3342 949 587 248
BbsI GAAGACnn'nnnn_
Cuts at: 182 1997 182
Size: 1815 3311
BbvCI CC'TCA_GC
Cuts at: 1065 1746 1065
Size: 681 4445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1627 1661 90
Size: 34 1503 34 3555
Fragments arranged by size:
3555 1503 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3024 3785 5126 3024
Size: 761 1341 3024
Fragments arranged by size:
3024 1341 761
BclI T'GATC_A
Cuts at: 2646 2646
Size: 5126
BglI GCCn_nnn'nGGC
Cuts at: 1060 1385 1060
Size: 325 4801
BglII A'GATC_T
Cuts at: 2641 4678 2641
Size: 2037 3089
BlpI GC'TnA_GC
Cuts at: 142 952 1722 2238 142
Size: 810 770 516 3030
Fragments arranged by size:
3030 810 770 516
Bme1580I G_kGCm'C
Cuts at: 118 215 485 1035 1603 2751 2844 3900 118
Size: 97 270 550 568 1148 93 1056 1344
Fragments arranged by size:
1344 1148 1056 568 550 270 97 93
BmgBI CAC'GTC
Cuts at: 1694 1694
Size: 5126
BmrI ACTGGGnnnn_n'
Cuts at: 869 1485 2338 2419 2754 4898 869
Size: 616 853 81 335 2144 1097
Fragments arranged by size:
2144 1097 853 616 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 924 3493 924
Size: 2569 2557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 767 952 1062 1092 1381 1489 1534 2020
Size: 610 185 110 30 289 108 45 486
Cuts at: 2020 157
Size: 3263
Fragments arranged by size:
3263 610 486 289 185 110 108 45
30
Bpu10I CC'TnA_GC
Cuts at: 1065 1436 1746 1065
Size: 371 310 4445
Fragments arranged by size:
4445 371 310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 2302 3181 3673 3971 1252
Size: 1050 879 492 298 2407
Fragments arranged by size:
2407 1050 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 617 982 1190 4830 617
Size: 365 208 3640 913
Fragments arranged by size:
3640 913 365 208
BsaAI yAC'GTr
Cuts at: 425 1635 2479 425
Size: 1210 844 3072
Fragments arranged by size:
3072 1210 844
BsaBI GATnn'nnATC
Cuts at: 6 2640 4677 6
Size: 2634 2037 455
Fragments arranged by size:
2634 2037 455
BsaHI Gr'CG_yC
Cuts at: 792 1021 1608 2557 2805 3507 5003 792
Size: 229 587 949 248 702 1496 915
Fragments arranged by size:
1496 949 915 702 587 248 229
BsaWI w'CCGG_w
Cuts at: 221 548 572 2315 2836 3514 3788 3935 4346
Size: 327 24 1743 521 678 274 147 411
Cuts at: 4346 221
Size: 1001
Fragments arranged by size:
1743 1001 678 521 411 327 274 147
24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1469 1499 1643 1673 1723 1753 1908 1938 1469
Size: 30 144 30 50 30 155 30 4657
Fragments arranged by size:
4657 155 144 50 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 625 1911 625
Size: 1286 3840
BseYI C'CCAG_C
Cuts at: 136 317 398 458 644 698 911 1227 1682
Size: 181 81 60 186 54 213 316 455
Cuts at: 1682 1735 1761 1894 2551 3309 3886 4382 136
Size: 53 26 133 657 758 577 496 880
Fragments arranged by size:
880 758 657 577 496 455 316 213
186 181 133 81 60 54 53 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1657 1657
Size: 5126
BsiEI CG_ry'CG
Cuts at: 104 1055 1380 2668 2714 3922 104
Size: 951 325 1288 46 1208 1308
Fragments arranged by size:
1308 1288 1208 951 325 46
BsiHKAI G_wGCw'C
Cuts at: 788 1015 1078 1373 1547 2140 2918 3108 3900
Size: 227 63 295 174 593 778 190 792
Cuts at: 3900 788
Size: 2014
Fragments arranged by size:
2014 792 778 593 295 227 190 174
63
BsmI GAATG_Cn'
Cuts at: 751 751
Size: 5126
BsmBI CGTCTCn'nnnn_
Cuts at: 1700 1700
Size: 5126
Bsp1286I G_dGCh'C
Cuts at: 118 143 215 485 645 788 1015 1035 1078
Size: 25 72 270 160 143 227 20 43
Cuts at: 1078 1373 1547 1603 2140 2751 2844 2918 3108
Size: 295 174 56 537 611 93 74 190
Cuts at: 3108 3170 3900 118
Size: 62 730 1344
Fragments arranged by size:
1344 730 611 537 295 270 227 190
174 160 143 93 74 72 62 56
43 25 20
BspEI T'CCGG_A
Cuts at: 221 4346 221
Size: 4125 1001
BspHI T'CATG_A
Cuts at: 4302 4302
Size: 5126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 663 983 1629 2176 2390 2692 3073 663
Size: 320 646 547 214 302 381 2716
Fragments arranged by size:
2716 646 547 381 320 302 214
BsrBI CCG'CTC
Cuts at: 50 497 1721 3418 3472 4956 5121 50
Size: 447 1224 1697 54 1484 165 55
Fragments arranged by size:
1697 1484 1224 447 165 55 54
BsrDI GCAATG_nn'
Cuts at: 291 506 3038 291
Size: 215 2532 2379
Fragments arranged by size:
2532 2379 215
BsrFI r'CCGG_y
Cuts at: 1017 1054 3124 3305 3514 1017
Size: 37 2070 181 209 2629
Fragments arranged by size:
2629 2070 209 181 37
BssSI C'ACGA_G
Cuts at: 1646 3397 3755 1646
Size: 1751 358 3017
Fragments arranged by size:
3017 1751 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2503 2503
Size: 5126
BstBI TT'CG_AA
Cuts at: 3487 3487
Size: 5126
BstXI CCAn_nnnn'nTGG
Cuts at: 1779 1855 1779
Size: 76 5050
BstYI r'GATC_y
Cuts at: 1 386 1430 2151 2618 2641 2976 3222 4234
Size: 385 1044 721 467 23 335 246 1012
Cuts at: 4234 4320 4332 4678 1
Size: 86 12 346 449
Fragments arranged by size:
1044 1012 721 467 449 385 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 485 1374 485
Size: 889 4237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 3076 3257 3420 1978
Size: 1098 181 163 3684
Fragments arranged by size:
3684 1098 181 163
BtsI GCAGTG_nn'
Cuts at: 2044 2369 2044
Size: 325 4801
DraI TTT'AAA
Cuts at: 2125 2125
Size: 5126
DrdI GACnn_nn'nnGTC
Cuts at: 825 2832 3690 825
Size: 2007 858 2261
Fragments arranged by size:
2261 2007 858
EaeI y'GGCC_r
Cuts at: 165 349 944 1052 1377 2711 2885 3276 3303
Size: 184 595 108 325 1334 174 391 27
Cuts at: 3303 4648 165
Size: 1345 643
Fragments arranged by size:
1345 1334 643 595 391 325 184 174
108 27
EagI C'GGCC_G
Cuts at: 1052 1377 2711 1052
Size: 325 1334 3467
Fragments arranged by size:
3467 1334 325
EarI CTCTTCn'nnn_
Cuts at: 707 818 1235 1243 3149 3359 707
Size: 111 417 8 1906 210 2474
Fragments arranged by size:
2474 1906 417 210 111 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1098 3334 3644 3790 4936 1098
Size: 2236 310 146 1146 1288
Fragments arranged by size:
2236 1288 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1013 1076 2138 1013
Size: 63 1062 4001
Fragments arranged by size:
4001 1062 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 731 767 952 1062 1092 1381 1489 1534
Size: 574 36 185 110 30 289 108 45
Cuts at: 1534 2020 2951 3383 4130 4398 157
Size: 486 931 432 747 268 885
Fragments arranged by size:
931 885 747 574 486 432 289 268
185 110 108 45 36 30
EcoO109I rG'GnC_Cy
Cuts at: 114 355 972 1032 1509 1891 1916 1979 2075
Size: 241 617 60 477 382 25 63 96
Cuts at: 2075 2265 114
Size: 190 2975
Fragments arranged by size:
2975 617 477 382 241 190 96 63
60 25
EcoRI G'AATT_C
Cuts at: 2130 2130
Size: 5126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1293 3145 1293
Size: 1852 3274
FseI GG_CCGG'CC
Cuts at: 1058 1058
Size: 5126
FspI TGC'GCA
Cuts at: 1756 2907 1756
Size: 1151 3975
HaeII r_GCGC'y
Cuts at: 459 959 1024 1611 2560 2808 3830 4809 459
Size: 500 65 587 949 248 1022 979 776
Fragments arranged by size:
1022 979 949 776 587 500 248 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1532 1564 1836 1868 1908 1940 2968 2998 3000
Size: 32 272 32 40 32 1028 30 2
Cuts at: 3000 3030 1532
Size: 30 3628
Fragments arranged by size:
3628 1028 272 40 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 528 2165 528
Size: 1637 3489
KasI G'GCGC_C
Cuts at: 1020 1607 2556 2804 1020
Size: 587 949 248 3342
Fragments arranged by size:
3342 949 587 248
KpnI G_GTAC'C
Cuts at: 549 2146 549
Size: 1597 3529
MfeI C'AATT_G
Cuts at: 983 983
Size: 5126
MluI A'CGCG_T
Cuts at: 4559 4559
Size: 5126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 260 1144 3797 3981 260
Size: 884 2653 184 1405
Fragments arranged by size:
2653 1405 884 184
MscI TGG'CCA
Cuts at: 167 351 2887 167
Size: 184 2536 2406
Fragments arranged by size:
2536 2406 184
MslI CAynn'nnrTG
Cuts at: 922 3115 3242 922
Size: 2193 127 2806
Fragments arranged by size:
2806 2193 127
MspA1I CmG'CkG
Cuts at: 594 882 1044 1711 1765 2237 2551 2911 3924
Size: 288 162 667 54 472 314 360 1013
Cuts at: 3924 4169 594
Size: 245 1551
Fragments arranged by size:
1551 1013 667 472 360 314 288 245
162 54
NaeI GCC'GGC
Cuts at: 1019 1056 3307 1019
Size: 37 2251 2838
Fragments arranged by size:
2838 2251 37
NarI GG'CG_CC
Cuts at: 1021 1608 2557 2805 1021
Size: 587 949 248 3342
Fragments arranged by size:
3342 949 587 248
NgoMIV G'CCGG_C
Cuts at: 1017 1054 3305 1017
Size: 37 2251 2838
Fragments arranged by size:
2838 2251 37
NspI r_CATG'y
Cuts at: 504 1093 2179 2679 3586 504
Size: 589 1086 500 907 2044
Fragments arranged by size:
2044 1086 907 589 500
PasI CC'CwG_GG
Cuts at: 358 358
Size: 5126
PciI A'CATG_T
Cuts at: 3582 3582
Size: 5126
PflMI CCAn_nnn'nTGG
Cuts at: 358 542 1134 1467 1905 358
Size: 184 592 333 438 3579
Fragments arranged by size:
3579 592 438 333 184
PfoI T'CCnGG_A
Cuts at: 829 2068 829
Size: 1239 3887
PmeI GTTT'AAAC
Cuts at: 2125 2125
Size: 5126
PmlI CAC'GTG
Cuts at: 425 425
Size: 5126
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1643 1675 4265 4297 1643
Size: 32 2590 32 2472
Fragments arranged by size:
2590 2472 32 32
PpuMI rG'GwC_Cy
Cuts at: 972 1509 1891 1916 2075 972
Size: 537 382 25 159 4023
Fragments arranged by size:
4023 537 382 159 25
PshAI GACnn'nnGTC
Cuts at: 999 999
Size: 5126
PspOMI G'GGCC_C
Cuts at: 114 481 1031 114
Size: 367 550 4209
Fragments arranged by size:
4209 550 367
PstI C_TGCA'G
Cuts at: 770 848 1313 2173 2858 770
Size: 78 465 860 685 3038
Fragments arranged by size:
3038 860 685 465 78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5126
PvuII CAG'CTG
Cuts at: 594 1765 2551 2911 594
Size: 1171 786 360 2809
Fragments arranged by size:
2809 1171 786 360
RsrII CG'GwC_CG
Cuts at: 3321 3321
Size: 5126
SacI G_AGCT'C
Cuts at: 1015 1078 2140 1015
Size: 63 1062 4001
Fragments arranged by size:
4001 1062 63
SalI G'TCGA_C
Cuts at: 2163 2163
Size: 5126
SapI GCTCTTCn'nnn_
Cuts at: 3149 3359 3149
Size: 210 4916
SbfI CC_TGCA'GG
Cuts at: 2173 2173
Size: 5126
ScaI AGT'ACT
Cuts at: 4643 4643
Size: 5126
SexAI A'CCwGG_T
Cuts at: 1460 1460
Size: 5126
SfcI C'TryA_G
Cuts at: 33 738 766 844 1309 2169 2854 3847 4038
Size: 705 28 78 465 860 685 993 191
Cuts at: 4038 33
Size: 1121
Fragments arranged by size:
1121 993 860 705 685 465 191 78
28
SfiI GGCCn_nnn'nGGCC
Cuts at: 1060 1060
Size: 5126
SfoI GGC'GCC
Cuts at: 1022 1609 2558 2806 1022
Size: 587 949 248 3342
Fragments arranged by size:
3342 949 587 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5126
SgrAI Cr'CCGG_yG
Cuts at: 1017 1017
Size: 5126
SmaI CCC'GGG
Cuts at: 199 2148 199
Size: 1949 3177
SmlI C'TyrA_G
Cuts at: 1267 1886 2281 3196 3688 3950 1267
Size: 619 395 915 492 262 2443
Fragments arranged by size:
2443 915 619 492 395 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5126
SphI G_CATG'C
Cuts at: 504 2179 2679 504
Size: 1675 500 2951
Fragments arranged by size:
2951 1675 500
StyI C'CwwG_G
Cuts at: 431 1158 1295 1611 2260 2614 431
Size: 727 137 316 649 354 2943
Fragments arranged by size:
2943 727 649 354 316 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 633 1840 2050 2709 2842 3301 3510 421
Size: 212 1207 210 659 133 459 209 2037
Fragments arranged by size:
2037 1207 659 459 212 210 209 133
TatI w'GTAC_w
Cuts at: 1493 1973 4641 1493
Size: 480 2668 1978
Fragments arranged by size:
2668 1978 480
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 160 192 319 351 1469 1501 1723 1755 1851
Size: 32 127 32 1118 32 222 32 96
Cuts at: 1851 1883 4643 4675 160
Size: 32 2760 32 611
Fragments arranged by size:
2760 1118 611 222 127 96 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 811 1174 1324 2923 811
Size: 363 150 1599 3014
Fragments arranged by size:
3014 1599 363 150
XbaI T'CTAG_A
Cuts at: 2157 2157
Size: 5126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 879 1902 2621 879
Size: 1023 719 3384
Fragments arranged by size:
3384 1023 719
XhoI C'TCGA_G
Cuts at: 1886 1886
Size: 5126
XmaI C'CCGG_G
Cuts at: 197 2146 197
Size: 1949 3177
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI BaeI BfrBI BmtI
BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PsiI PspXI PsrI
SacII SanDI SnaBI SrfI SspI StuI SwaI XmnI
ZraI