Restriction map for
pF1KB6214
(Circular) (Six-base) MAPSORT of: pF1KB6214.seq Check: 1832 from: 1 to: 5288
pF1KB6214 5288 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 2326 2326
Size: 5288
Acc65I G'GTAC_C
Cuts at: 1476 2304 1476
Size: 828 4460
AclI AA'CG_TT
Cuts at: 5128 5128
Size: 5288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 563 679 1865 3113 3545 4292 4560 367
Size: 196 116 1186 1248 432 747 268 1095
Fragments arranged by size:
1248 1186 1095 747 432 268 196 116
AflIII A'CryG_T
Cuts at: 2640 3744 4721 2640
Size: 1104 977 3207
Fragments arranged by size:
3207 1104 977
AgeI A'CCGG_T
Cuts at: 3676 3676
Size: 5288
AleI CACnn'nnGTG
Cuts at: 1696 1696
Size: 5288
AlwNI CAG_nnn'CTG
Cuts at: 2593 4160 2593
Size: 1567 3721
ApaI G_GGCC'C
Cuts at: 305 305
Size: 5288
ApaLI G'TGCA_C
Cuts at: 4058 4058
Size: 5288
ApoI r'AATT_y
Cuts at: 1441 1668 2292 4641 4897 1441
Size: 227 624 2349 256 1832
Fragments arranged by size:
2349 1832 624 256 227
AseI AT'TA_AT
Cuts at: 20 20
Size: 5288
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5288
AvaI C'yCGr_G
Cuts at: 530 2308 530
Size: 1778 3510
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1069 1102 1069
Size: 33 5255
BamHI G'GATC_C
Cuts at: 2313 2313
Size: 5288
BanI G'GyrC_C
Cuts at: 1476 2304 2718 2966 3001 1476
Size: 828 414 248 35 3763
Fragments arranged by size:
3763 828 414 248 35
BanII G_rGCy'C
Cuts at: 305 2302 3332 305
Size: 1997 1030 2261
Fragments arranged by size:
2261 1997 1030
BbeI G_GCGC'C
Cuts at: 2722 2970 2722
Size: 248 5040
BbsI GAAGACnn'nnnn_
Cuts at: 1289 1289
Size: 5288
BciVI GTATCCnnnnn_n'
Cuts at: 579 1887 2067 3186 3947 5288 579
Size: 1308 180 1119 761 1341 579
Fragments arranged by size:
1341 1308 1119 761 579 180
BclI T'GATC_A
Cuts at: 2808 2808
Size: 5288
BglII A'GATC_T
Cuts at: 2803 4840 2803
Size: 2037 3251
BlpI GC'TnA_GC
Cuts at: 2400 2400
Size: 5288
Bme1580I G_kGCm'C
Cuts at: 305 2913 3006 4062 305
Size: 2608 93 1056 1531
Fragments arranged by size:
2608 1531 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 548 894 2500 2581 2916 5060 548
Size: 346 1606 81 335 2144 776
Fragments arranged by size:
2144 1606 776 346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3655 3655
Size: 5288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 189
Size: 5288
Bpu10I CC'TnA_GC
Cuts at: 1733 1733
Size: 5288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 1349 1575 2464 3343 3835 4133 158
Size: 1191 226 889 879 492 298 1313
Fragments arranged by size:
1313 1191 889 879 492 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 1633 4992 1633
Size: 3359 1929
BsaAI yAC'GTr
Cuts at: 2641 2641
Size: 5288
BsaBI GATnn'nnATC
Cuts at: 6 2027 2802 4839 6
Size: 2021 775 2037 455
Fragments arranged by size:
2037 2021 775 455
BsaHI Gr'CG_yC
Cuts at: 2719 2967 3669 5165 2719
Size: 248 702 1496 2842
Fragments arranged by size:
2842 1496 702 248
BsaWI w'CCGG_w
Cuts at: 661 2477 2998 3676 3950 4097 4508 661
Size: 1816 521 678 274 147 411 1441
Fragments arranged by size:
1816 1441 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 952 982 1556 1586 1735 1765 952
Size: 30 574 30 149 30 4475
Fragments arranged by size:
4475 574 149 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1743 1752 1743
Size: 9 5279
BseYI C'CCAG_C
Cuts at: 864 2713 3471 4048 4544 864
Size: 1849 758 577 496 1608
Fragments arranged by size:
1849 1608 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2143 2143
Size: 5288
BsiEI CG_ry'CG
Cuts at: 104 2830 2876 4084 104
Size: 2726 46 1208 1308
Fragments arranged by size:
2726 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 174 2302 3080 3270 4062 174
Size: 2128 778 190 792 1400
Fragments arranged by size:
2128 1400 792 778 190
BsmI GAATG_Cn'
Cuts at: 671 671
Size: 5288
Bsp1286I G_dGCh'C
Cuts at: 174 305 2302 2913 3006 3080 3270 3332 4062
Size: 131 1997 611 93 74 190 62 730
Cuts at: 4062 174
Size: 1400
Fragments arranged by size:
1997 1400 730 611 190 131 93 74
62
BspEI T'CCGG_A
Cuts at: 4508 4508
Size: 5288
BspHI T'CATG_A
Cuts at: 1245 4464 1245
Size: 3219 2069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1013 2338 2552 2854 3235 1013
Size: 1325 214 302 381 3066
Fragments arranged by size:
3066 1325 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3580 3634 5118 5283 50
Size: 3530 54 1484 165 55
Fragments arranged by size:
3530 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 640 2045 3200 640
Size: 1405 1155 2728
Fragments arranged by size:
2728 1405 1155
BsrFI r'CCGG_y
Cuts at: 3286 3467 3676 3286
Size: 181 209 4898
Fragments arranged by size:
4898 209 181
BsrGI T'GTAC_A
Cuts at: 1212 1212
Size: 5288
BssSI C'ACGA_G
Cuts at: 260 3559 3917 260
Size: 3299 358 1631
Fragments arranged by size:
3299 1631 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2665 2665
Size: 5288
BstBI TT'CG_AA
Cuts at: 3649 3649
Size: 5288
BstEII G'GTnAC_C
Cuts at: 2005 2005
Size: 5288
BstXI CCAn_nnnn'nTGG
Cuts at: 1698 1698
Size: 5288
BstYI r'GATC_y
Cuts at: 1 2313 2780 2803 3138 3384 4396 4482 4494
Size: 2312 467 23 335 246 1012 86 12
Cuts at: 4494 4840 1
Size: 346 449
Fragments arranged by size:
2312 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 500 500
Size: 5288
BtgI C'CryG_G
Cuts at: 107 768 2000 107
Size: 661 1232 3395
Fragments arranged by size:
3395 1232 661
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3238 3419 3582 3238
Size: 181 163 4944
Fragments arranged by size:
4944 181 163
BtsI GCAGTG_nn'
Cuts at: 2531 2531
Size: 5288
DraI TTT'AAA
Cuts at: 2287 2287
Size: 5288
DraIII CAC_nnn'GTG
Cuts at: 835 835
Size: 5288
DrdI GACnn_nn'nnGTC
Cuts at: 852 2994 3852 852
Size: 2142 858 2288
Fragments arranged by size:
2288 2142 858
EaeI y'GGCC_r
Cuts at: 2873 3047 3438 3465 4810 2873
Size: 174 391 27 1345 3351
Fragments arranged by size:
3351 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2873 2873
Size: 5288
EarI CTCTTCn'nnn_
Cuts at: 149 539 698 1304 3311 3521 149
Size: 390 159 606 2007 210 1916
Fragments arranged by size:
2007 1916 606 390 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3496 3806 3952 5098 3496
Size: 310 146 1146 3686
Fragments arranged by size:
3686 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2300 2300
Size: 5288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 367 563 679 1865 3113 3545 4292 4560
Size: 178 196 116 1186 1248 432 747 268
Cuts at: 4560 189
Size: 917
Fragments arranged by size:
1248 1186 917 747 432 268 196 178
116
EcoO109I rG'GnC_Cy
Cuts at: 301 302 688 845 2427 301
Size: 1 386 157 1582 3162
Fragments arranged by size:
3162 1582 386 157 1
EcoRI G'AATT_C
Cuts at: 2292 2292
Size: 5288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 697 1043 1194 3307 697
Size: 346 151 2113 2678
Fragments arranged by size:
2678 2113 346 151
FspI TGC'GCA
Cuts at: 3069 3069
Size: 5288
HaeII r_GCGC'y
Cuts at: 2722 2970 3992 4971 2722
Size: 248 1022 979 3039
Fragments arranged by size:
3039 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 306 338 483 515 2140 2172 3130 3160 3162
Size: 32 145 32 1625 32 958 30 2
Cuts at: 3162 3192 306
Size: 30 2402
Fragments arranged by size:
2402 1625 958 145 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 138 2327 138
Size: 2189 3099
HindIII A'AGCT_T
Cuts at: 1154 1154
Size: 5288
KasI G'GCGC_C
Cuts at: 2718 2966 2718
Size: 248 5040
KpnI G_GTAC'C
Cuts at: 1480 2308 1480
Size: 828 4460
MluI A'CGCG_T
Cuts at: 4721 4721
Size: 5288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1531 2040 3959 4143 1531
Size: 509 1919 184 2676
Fragments arranged by size:
2676 1919 509 184
MscI TGG'CCA
Cuts at: 3049 3049
Size: 5288
MslI CAynn'nnrTG
Cuts at: 720 1535 1696 2194 3277 3404 720
Size: 815 161 498 1083 127 2604
Fragments arranged by size:
2604 1083 815 498 161 127
MspA1I CmG'CkG
Cuts at: 1843 2399 2713 3073 4086 4331 1843
Size: 556 314 360 1013 245 2800
Fragments arranged by size:
2800 1013 556 360 314 245
NaeI GCC'GGC
Cuts at: 3469 3469
Size: 5288
NarI GG'CG_CC
Cuts at: 2719 2967 2719
Size: 248 5040
NcoI C'CATG_G
Cuts at: 107 2000 107
Size: 1893 3395
NdeI CA'TA_TG
Cuts at: 912 912
Size: 5288
NgoMIV G'CCGG_C
Cuts at: 3467 3467
Size: 5288
NspI r_CATG'y
Cuts at: 1167 1970 2341 2841 3748 1167
Size: 803 371 500 907 2707
Fragments arranged by size:
2707 907 803 500 371
PciI A'CATG_T
Cuts at: 3744 3744
Size: 5288
PflMI CCAn_nnn'nTGG
Cuts at: 348 916 348
Size: 568 4720
PmeI GTTT'AAAC
Cuts at: 2287 2287
Size: 5288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 4427
Size: 32 5256
PpuMI rG'GwC_Cy
Cuts at: 845 845
Size: 5288
PspOMI G'GGCC_C
Cuts at: 301 301
Size: 5288
PstI C_TGCA'G
Cuts at: 333 1323 2335 3020 333
Size: 990 1012 685 2601
Fragments arranged by size:
2601 1012 990 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5288
PvuII CAG'CTG
Cuts at: 1843 2713 3073 1843
Size: 870 360 4058
Fragments arranged by size:
4058 870 360
RsrII CG'GwC_CG
Cuts at: 3483 3483
Size: 5288
SacI G_AGCT'C
Cuts at: 2302 2302
Size: 5288
SalI G'TCGA_C
Cuts at: 2325 2325
Size: 5288
SanDI GG'GwC_CC
Cuts at: 845 845
Size: 5288
SapI GCTCTTCn'nnn_
Cuts at: 3311 3521 3311
Size: 210 5078
SbfI CC_TGCA'GG
Cuts at: 2335 2335
Size: 5288
ScaI AGT'ACT
Cuts at: 1989 4805 1989
Size: 2816 2472
SfcI C'TryA_G
Cuts at: 33 239 329 1106 1319 1613 1792 2279 2331
Size: 206 90 777 213 294 179 487 52
Cuts at: 2331 3016 4009 4200 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 777 685 487 294 213 206
191 179 90 52
SfoI GGC'GCC
Cuts at: 2720 2968 2720
Size: 248 5040
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5288
SmaI CCC'GGG
Cuts at: 2310 2310
Size: 5288
SmlI C'TyrA_G
Cuts at: 173 1364 1554 2443 3358 3850 4112 173
Size: 1191 190 889 915 492 262 1349
Fragments arranged by size:
1349 1191 915 889 492 262 190
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5288
SphI G_CATG'C
Cuts at: 2341 2841 2341
Size: 500 4788
SspI AAT'ATT
Cuts at: 1629 1781 1629
Size: 152 5136
StyI C'CwwG_G
Cuts at: 107 1813 2000 2422 2776 107
Size: 1706 187 422 354 2619
Fragments arranged by size:
2619 1706 422 354 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2871 3004 3463 3672 2871
Size: 133 459 209 4487
Fragments arranged by size:
4487 459 209 133
TatI w'GTAC_w
Cuts at: 1212 1987 4803 1212
Size: 775 2816 1697
Fragments arranged by size:
2816 1697 775
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1735 1767 4805 4837 1735
Size: 32 3038 32 2186
Fragments arranged by size:
3038 2186 32 32
Tth111I GACn'n_nGTC
Cuts at: 3085 3085
Size: 5288
XbaI T'CTAG_A
Cuts at: 2319 2319
Size: 5288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2783 2783
Size: 5288
XmaI C'CCGG_G
Cuts at: 2308 2308
Size: 5288
XmnI GAAnn'nnTTC
Cuts at: 680 680
Size: 5288
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BssHII BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI