Restriction map for
pF1KB6189
(Circular) (Six-base) MAPSORT of: pF1KB6189.seq Check: 3069 from: 1 to: 5093
pF1KB6189 5093 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 869 1164 869
Size: 295 4798
AatII G_ACGT'C
Cuts at: 1091 1091
Size: 5093
AccI GT'mk_AC
Cuts at: 2131 2131
Size: 5093
Acc65I G'GTAC_C
Cuts at: 2109 2109
Size: 5093
AclI AA'CG_TT
Cuts at: 4933 4933
Size: 5093
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 898 2918 3350 4097 4365 277
Size: 621 2020 432 747 268 1005
Fragments arranged by size:
2020 1005 747 621 432 268
AflII C'TTAA_G
Cuts at: 603 603
Size: 5093
AflIII A'CryG_T
Cuts at: 1468 2000 2445 3549 4526 1468
Size: 532 445 1104 977 2035
Fragments arranged by size:
2035 1104 977 532 445
AgeI A'CCGG_T
Cuts at: 188 1640 3481 188
Size: 1452 1841 1800
Fragments arranged by size:
1841 1800 1452
AhdI GACnn_n'nnGTC
Cuts at: 419 419
Size: 5093
AlwNI CAG_nnn'CTG
Cuts at: 2032 2398 3965 2032
Size: 366 1567 3160
Fragments arranged by size:
3160 1567 366
ApaLI G'TGCA_C
Cuts at: 1348 3863 1348
Size: 2515 2578
ApoI r'AATT_y
Cuts at: 225 249 784 871 961 1150 2097 4446 4702
Size: 24 535 87 90 189 947 2349 256
Cuts at: 4702 225
Size: 616
Fragments arranged by size:
2349 947 616 535 256 189 90 87
24
AseI AT'TA_AT
Cuts at: 20 593 20
Size: 573 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
AvaI C'yCGr_G
Cuts at: 1409 1784 2113 1409
Size: 375 329 4389
Fragments arranged by size:
4389 375 329
BamHI G'GATC_C
Cuts at: 2118 2118
Size: 5093
BanI G'GyrC_C
Cuts at: 2109 2523 2771 2806 2109
Size: 414 248 35 4396
Fragments arranged by size:
4396 414 248 35
BanII G_rGCy'C
Cuts at: 2107 3137 2107
Size: 1030 4063
BbeI G_GCGC'C
Cuts at: 2527 2775 2527
Size: 248 4845
BbsI GAAGACnn'nnnn_
Cuts at: 283 631 1407 1454 283
Size: 348 776 47 3922
Fragments arranged by size:
3922 776 348 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 834 868 834
Size: 34 5059
BciVI GTATCCnnnnn_n'
Cuts at: 2991 3752 5093 2991
Size: 761 1341 2991
Fragments arranged by size:
2991 1341 761
BclI T'GATC_A
Cuts at: 2613 2613
Size: 5093
BfrBI ATG'CAT
Cuts at: 729 1081 729
Size: 352 4741
BglII A'GATC_T
Cuts at: 2608 4645 2608
Size: 2037 3056
BlpI GC'TnA_GC
Cuts at: 2205 2205
Size: 5093
Bme1580I G_kGCm'C
Cuts at: 1352 2718 2811 3867 1352
Size: 1366 93 1056 2578
Fragments arranged by size:
2578 1366 1056 93
BmgBI CAC'GTC
Cuts at: 801 1744 801
Size: 943 4150
BmrI ACTGGGnnnn_n'
Cuts at: 831 837 2305 2386 2721 4865 831
Size: 6 1468 81 335 2144 1059
Fragments arranged by size:
2144 1468 1059 335 81 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3460 3460
Size: 5093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 982
Size: 5093
Bpu10I CC'TnA_GC
Cuts at: 805 805
Size: 5093
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2269 3148 3640 3938 2269
Size: 879 492 298 3424
Fragments arranged by size:
3424 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4797 4797
Size: 5093
BsaAI yAC'GTr
Cuts at: 2446 2446
Size: 5093
BsaBI GATnn'nnATC
Cuts at: 6 2607 4644 6
Size: 2601 2037 455
Fragments arranged by size:
2601 2037 455
BsaHI Gr'CG_yC
Cuts at: 1088 2524 2772 3474 4970 1088
Size: 1436 248 702 1496 1211
Fragments arranged by size:
1496 1436 1211 702 248
BsaWI w'CCGG_w
Cuts at: 188 1640 2282 2803 3481 3755 3902 4313 188
Size: 1452 642 521 678 274 147 411 968
Fragments arranged by size:
1452 968 678 642 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 688 718 1645 1675 688
Size: 30 927 30 4106
Fragments arranged by size:
4106 927 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 392 970 392
Size: 578 4515
BseYI C'CCAG_C
Cuts at: 2518 3276 3853 4349 2518
Size: 758 577 496 3262
Fragments arranged by size:
3262 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 1192
Size: 5093
BsiEI CG_ry'CG
Cuts at: 104 2635 2681 3889 104
Size: 2531 46 1208 1308
Fragments arranged by size:
2531 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 133 806 1352 2107 2885 3075 3867 133
Size: 673 546 755 778 190 792 1359
Fragments arranged by size:
1359 792 778 755 673 546 190
BsmI GAATG_Cn'
Cuts at: 1587 1587
Size: 5093
BsmBI CGTCTCn'nnnn_
Cuts at: 1095 1095
Size: 5093
Bsp1286I G_dGCh'C
Cuts at: 133 806 1352 2107 2718 2811 2885 3075 3137
Size: 673 546 755 611 93 74 190 62
Cuts at: 3137 3867 133
Size: 730 1359
Fragments arranged by size:
1359 755 730 673 611 546 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4313 4313
Size: 5093
BspHI T'CATG_A
Cuts at: 1809 4269 1809
Size: 2460 2633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 672 869 1164 2143 2357 2659 3040 672
Size: 197 295 979 214 302 381 2725
Fragments arranged by size:
2725 979 381 302 295 214 197
BsrBI CCG'CTC
Cuts at: 50 3385 3439 4923 5088 50
Size: 3335 54 1484 165 55
Fragments arranged by size:
3335 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3005 3005
Size: 5093
BsrFI r'CCGG_y
Cuts at: 188 1640 1832 3091 3272 3481 188
Size: 1452 192 1259 181 209 1800
Fragments arranged by size:
1800 1452 1259 209 192 181
BssSI C'ACGA_G
Cuts at: 3364 3722 3364
Size: 358 4735
BstAPI GCAn_nnn'nTGC
Cuts at: 2032 2470 2032
Size: 438 4655
BstBI TT'CG_AA
Cuts at: 282 3454 282
Size: 3172 1921
BstYI r'GATC_y
Cuts at: 1 909 1497 2118 2585 2608 2943 3189 4201
Size: 908 588 621 467 23 335 246 1012
Cuts at: 4201 4287 4299 4645 1
Size: 86 12 346 449
Fragments arranged by size:
1012 908 621 588 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 742 742
Size: 5093
BtgI C'CryG_G
Cuts at: 107 1526 107
Size: 1419 3674
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 837 3043 3224 3387 837
Size: 2206 181 163 2543
Fragments arranged by size:
2543 2206 181 163
BtsI GCAGTG_nn'
Cuts at: 2336 2336
Size: 5093
DraI TTT'AAA
Cuts at: 254 1464 2092 254
Size: 1210 628 3255
Fragments arranged by size:
3255 1210 628
DrdI GACnn_nn'nnGTC
Cuts at: 2799 3657 2799
Size: 858 4235
EaeI y'GGCC_r
Cuts at: 1748 2042 2678 2852 3243 3270 4615 1748
Size: 294 636 174 391 27 1345 2226
Fragments arranged by size:
2226 1345 636 391 294 174 27
EagI C'GGCC_G
Cuts at: 2678 2678
Size: 5093
EarI CTCTTCn'nnn_
Cuts at: 152 934 3116 3326 152
Size: 782 2182 210 1919
Fragments arranged by size:
2182 1919 782 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3301 3611 3757 4903 3301
Size: 310 146 1146 3491
Fragments arranged by size:
3491 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2105 2105
Size: 5093
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 898 982 2918 3350 4097 4365 277
Size: 621 84 1936 432 747 268 1005
Fragments arranged by size:
1936 1005 747 621 432 268 84
EcoNI CCTnn'n_nnAGG
Cuts at: 349 349
Size: 5093
EcoO109I rG'GnC_Cy
Cuts at: 833 2232 833
Size: 1399 3694
EcoRI G'AATT_C
Cuts at: 2097 2097
Size: 5093
EcoRV GAT'ATC
Cuts at: 2058 2058
Size: 5093
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3112 3112
Size: 5093
FspI TGC'GCA
Cuts at: 2874 2874
Size: 5093
HaeII r_GCGC'y
Cuts at: 2527 2775 3797 4776 2527
Size: 248 1022 979 2844
Fragments arranged by size:
2844 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 153 183 185 405 437 604 636 688
Size: 2 30 2 220 32 167 32 52
Cuts at: 688 720 1544 1576 2935 2965 2967 2997 151
Size: 32 824 32 1359 30 2 30 2247
Fragments arranged by size:
2247 1359 824 220 167 52 32 32
32 32 30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 696 749 2132 696
Size: 53 1383 3657
Fragments arranged by size:
3657 1383 53
HindIII A'AGCT_T
Cuts at: 1025 1033 1025
Size: 8 5085
KasI G'GCGC_C
Cuts at: 2523 2771 2523
Size: 248 4845
KpnI G_GTAC'C
Cuts at: 2113 2113
Size: 5093
MfeI C'AATT_G
Cuts at: 628 628
Size: 5093
MluI A'CGCG_T
Cuts at: 4526 4526
Size: 5093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3764 3948 3764
Size: 184 4909
MscI TGG'CCA
Cuts at: 1750 2044 2854 1750
Size: 294 810 3989
Fragments arranged by size:
3989 810 294
MslI CAynn'nnrTG
Cuts at: 981 3082 3209 981
Size: 2101 127 2865
Fragments arranged by size:
2865 2101 127
MspA1I CmG'CkG
Cuts at: 1699 2204 2518 2878 3891 4136 1699
Size: 505 314 360 1013 245 2656
Fragments arranged by size:
2656 1013 505 360 314 245
NaeI GCC'GGC
Cuts at: 3274 3274
Size: 5093
NarI GG'CG_CC
Cuts at: 2524 2772 2524
Size: 248 4845
NcoI C'CATG_G
Cuts at: 107 1526 107
Size: 1419 3674
NgoMIV G'CCGG_C
Cuts at: 3272 3272
Size: 5093
NsiI A_TGCA'T
Cuts at: 731 1083 731
Size: 352 4741
NspI r_CATG'y
Cuts at: 733 849 2004 2146 2646 3553 733
Size: 116 1155 142 500 907 2273
Fragments arranged by size:
2273 1155 907 500 142 116
PasI CC'CwG_GG
Cuts at: 1207 1207
Size: 5093
PciI A'CATG_T
Cuts at: 2000 3549 2000
Size: 1549 3544
PflMI CCAn_nnn'nTGG
Cuts at: 2085 2085
Size: 5093
PmeI GTTT'AAAC
Cuts at: 2092 2092
Size: 5093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 4232
Size: 32 5061
PpuMI rG'GwC_Cy
Cuts at: 833 833
Size: 5093
PsiI TTA'TAA
Cuts at: 926 926
Size: 5093
PspXI vC'TCGA_Gb
Cuts at: 1409 1409
Size: 5093
PstI C_TGCA'G
Cuts at: 149 1572 2140 2825 149
Size: 1423 568 685 2417
Fragments arranged by size:
2417 1423 685 568
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5093
PvuII CAG'CTG
Cuts at: 1699 2518 2878 1699
Size: 819 360 3914
Fragments arranged by size:
3914 819 360
RsrII CG'GwC_CG
Cuts at: 3288 3288
Size: 5093
SacI G_AGCT'C
Cuts at: 2107 2107
Size: 5093
SalI G'TCGA_C
Cuts at: 2130 2130
Size: 5093
SanDI GG'GwC_CC
Cuts at: 833 833
Size: 5093
SapI GCTCTTCn'nnn_
Cuts at: 3116 3326 3116
Size: 210 4883
SbfI CC_TGCA'GG
Cuts at: 2140 2140
Size: 5093
ScaI AGT'ACT
Cuts at: 4610 4610
Size: 5093
SfcI C'TryA_G
Cuts at: 33 145 1568 2136 2821 3814 4005 33
Size: 112 1423 568 685 993 191 1121
Fragments arranged by size:
1423 1121 993 685 568 191 112
SfoI GGC'GCC
Cuts at: 2525 2773 2525
Size: 248 4845
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
SmaI CCC'GGG
Cuts at: 2115 2115
Size: 5093
SmlI C'TyrA_G
Cuts at: 603 1409 1784 2248 3163 3655 3917 603
Size: 806 375 464 915 492 262 1779
Fragments arranged by size:
1779 915 806 492 464 375 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5093
SphI G_CATG'C
Cuts at: 849 2146 2646 849
Size: 1297 500 3296
Fragments arranged by size:
3296 1297 500
SspI AAT'ATT
Cuts at: 276 1364 1755 1988 276
Size: 1088 391 233 3381
Fragments arranged by size:
3381 1088 391 233
StyI C'CwwG_G
Cuts at: 107 488 1526 2045 2227 2581 107
Size: 381 1038 519 182 354 2619
Fragments arranged by size:
2619 1038 519 381 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2676 2809 3268 3477 2676
Size: 133 459 209 4292
Fragments arranged by size:
4292 459 209 133
TatI w'GTAC_w
Cuts at: 4608 4608
Size: 5093
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 433 465 1331 1363 1418 1450 4610 4642 433
Size: 32 866 32 55 32 3160 32 884
Fragments arranged by size:
3160 884 866 55 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1558 2890 1558
Size: 1332 3761
XbaI T'CTAG_A
Cuts at: 2124 2124
Size: 5093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1426 1800 2588 114
Size: 1312 374 788 2619
Fragments arranged by size:
2619 1312 788 374
XhoI C'TCGA_G
Cuts at: 1409 1784 1409
Size: 375 4718
XmaI C'CCGG_G
Cuts at: 2113 2113
Size: 5093
ZraI GAC'GTC
Cuts at: 1089 1089
Size: 5093
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PsiI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AfeI AleI AlfI AloI ApaI AscI AvrII BaeI
BbvCI BglI BmtI BsiWI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII FseI FspAI HpaI NdeI
NheI NotI NruI PacI PfoI PmlI PshAI PspOMI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XmnI