Restriction map for
pF1KB6188
(Circular) (Six-base) MAPSORT of: pF1KB6188.seq Check: 1331 from: 1 to: 5222
pF1KB6188 5222 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1919 1919
Size: 5222
AccI GT'mk_AC
Cuts at: 2260 2260
Size: 5222
Acc65I G'GTAC_C
Cuts at: 2238 2238
Size: 5222
AclI AA'CG_TT
Cuts at: 557 5062 557
Size: 4505 717
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 707 1004 1085 1105 1352 3047 3479 4226
Size: 361 297 81 20 247 1695 432 747
Cuts at: 4226 4494 346
Size: 268 1074
Fragments arranged by size:
1695 1074 747 432 361 297 268 247
81 20
AflIII A'CryG_T
Cuts at: 148 2574 3678 4655 148
Size: 2426 1104 977 715
Fragments arranged by size:
2426 1104 977 715
AgeI A'CCGG_T
Cuts at: 3610 3610
Size: 5222
AleI CACnn'nnGTG
Cuts at: 2065 2065
Size: 5222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 832 832
Size: 5222
AlwNI CAG_nnn'CTG
Cuts at: 1164 2161 2527 4094 1164
Size: 997 366 1567 2292
Fragments arranged by size:
2292 1567 997 366
ApaI G_GGCC'C
Cuts at: 787 1489 1710 2013 787
Size: 702 221 303 3996
Fragments arranged by size:
3996 702 303 221
ApaLI G'TGCA_C
Cuts at: 3992 3992
Size: 5222
ApoI r'AATT_y
Cuts at: 2226 4575 4831 2226
Size: 2349 256 2617
Fragments arranged by size:
2617 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5222
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5222
AvaI C'yCGr_G
Cuts at: 2013 2242 2013
Size: 229 4993
BamHI G'GATC_C
Cuts at: 2247 2247
Size: 5222
BanI G'GyrC_C
Cuts at: 495 1555 2238 2652 2900 2935 495
Size: 1060 683 414 248 35 2782
Fragments arranged by size:
2782 1060 683 414 248 35
BanII G_rGCy'C
Cuts at: 329 787 1271 1489 1499 1710 2013 2236 3266
Size: 458 484 218 10 211 303 223 1030
Cuts at: 3266 329
Size: 2285
Fragments arranged by size:
2285 1030 484 458 303 223 218 211
10
BbeI G_GCGC'C
Cuts at: 1559 2656 2904 1559
Size: 1097 248 3877
Fragments arranged by size:
3877 1097 248
BbsI GAAGACnn'nnnn_
Cuts at: 487 709 1305 1880 487
Size: 222 596 575 3829
Fragments arranged by size:
3829 596 575 222
BbvCI CC'TCA_GC
Cuts at: 406 406
Size: 5222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1801 1835 1801
Size: 34 5188
BciVI GTATCCnnnnn_n'
Cuts at: 3120 3881 5222 3120
Size: 761 1341 3120
Fragments arranged by size:
3120 1341 761
BclI T'GATC_A
Cuts at: 1103 2742 1103
Size: 1639 3583
BglI GCCn_nnn'nGGC
Cuts at: 987 1252 2173 2182 987
Size: 265 921 9 4027
Fragments arranged by size:
4027 921 265 9
BglII A'GATC_T
Cuts at: 357 2737 4774 357
Size: 2380 2037 805
Fragments arranged by size:
2380 2037 805
BlpI GC'TnA_GC
Cuts at: 127 172 2334 127
Size: 45 2162 3015
Fragments arranged by size:
3015 2162 45
Bme1580I G_kGCm'C
Cuts at: 787 1489 1710 2013 2096 2847 2940 3996 787
Size: 702 221 303 83 751 93 1056 2013
Fragments arranged by size:
2013 1056 751 702 303 221 93 83
BmgBI CAC'GTC
Cuts at: 300 300
Size: 5222
BmrI ACTGGGnnnn_n'
Cuts at: 195 931 940 1517 2434 2515 2850 4994 195
Size: 736 9 577 917 81 335 2144 423
Fragments arranged by size:
2144 917 736 577 423 335 81 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3589 3589
Size: 5222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 1186 1261 1453 1733 648
Size: 538 75 192 280 4137
Fragments arranged by size:
4137 538 280 192 75
Bpu10I CC'TnA_GC
Cuts at: 406 406
Size: 5222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 2398 3277 3769 4067 1255
Size: 1143 879 492 298 2410
Fragments arranged by size:
2410 1143 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1571 1984 4926 1571
Size: 413 2942 1867
Fragments arranged by size:
2942 1867 413
BsaAI yAC'GTr
Cuts at: 2575 2575
Size: 5222
BsaBI GATnn'nnATC
Cuts at: 6 2038 2074 2736 4773 6
Size: 2032 36 662 2037 455
Fragments arranged by size:
2037 2032 662 455 36
BsaHI Gr'CG_yC
Cuts at: 1556 2653 2901 3603 5099 1556
Size: 1097 248 702 1496 1679
Fragments arranged by size:
1679 1496 1097 702 248
BsaWI w'CCGG_w
Cuts at: 2411 2932 3610 3884 4031 4442 2411
Size: 521 678 274 147 411 3191
Fragments arranged by size:
3191 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 274 304 494 524 674 704 1927 1957 2170
Size: 30 190 30 150 30 1223 30 213
Cuts at: 2170 2200 274
Size: 30 3296
Fragments arranged by size:
3296 1223 213 190 150 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 390 651 700 792 1373 1590 2041 250
Size: 140 261 49 92 581 217 451 3431
Fragments arranged by size:
3431 581 451 261 217 140 92 49
BseYI C'CCAG_C
Cuts at: 747 895 1425 1499 1504 1661 1920 2647 3405
Size: 148 530 74 5 157 259 727 758
Cuts at: 3405 3982 4478 747
Size: 577 496 1491
Fragments arranged by size:
1491 758 727 577 530 496 259 157
148 74 5
BsiEI CG_ry'CG
Cuts at: 104 2764 2810 4018 104
Size: 2660 46 1208 1308
Fragments arranged by size:
2660 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 473 1271 2236 3014 3204 3996 473
Size: 798 965 778 190 792 1699
Fragments arranged by size:
1699 965 798 792 778 190
BsmI GAATG_Cn'
Cuts at: 1224 1846 1224
Size: 622 4600
BsmBI CGTCTCn'nnnn_
Cuts at: 430 430
Size: 5222
Bsp1286I G_dGCh'C
Cuts at: 329 473 787 1271 1489 1499 1710 2013 2096
Size: 144 314 484 218 10 211 303 83
Cuts at: 2096 2236 2847 2940 3014 3204 3266 3996 329
Size: 140 611 93 74 190 62 730 1555
Fragments arranged by size:
1555 730 611 484 314 303 218 211
190 144 140 93 83 74 62 10
BspEI T'CCGG_A
Cuts at: 4442 4442
Size: 5222
BspHI T'CATG_A
Cuts at: 4398 4398
Size: 5222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 1919 2272 2486 2788 3169 176
Size: 1743 353 214 302 381 2229
Fragments arranged by size:
2229 1743 381 353 302 214
BsrBI CCG'CTC
Cuts at: 50 1036 3514 3568 5052 5217 50
Size: 986 2478 54 1484 165 55
Fragments arranged by size:
2478 1484 986 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1325 2139 3134 1325
Size: 814 995 3413
Fragments arranged by size:
3413 995 814
BsrFI r'CCGG_y
Cuts at: 990 1610 3220 3401 3610 990
Size: 620 1610 181 209 2602
Fragments arranged by size:
2602 1610 620 209 181
BssSI C'ACGA_G
Cuts at: 3493 3851 3493
Size: 358 4864
BstAPI GCAn_nnn'nTGC
Cuts at: 2599 2599
Size: 5222
BstBI TT'CG_AA
Cuts at: 3583 3583
Size: 5222
BstXI CCAn_nnnn'nTGG
Cuts at: 1777 1777
Size: 5222
BstYI r'GATC_y
Cuts at: 1 357 1694 2247 2714 2737 3072 3318 4330
Size: 356 1337 553 467 23 335 246 1012
Cuts at: 4330 4416 4428 4774 1
Size: 86 12 346 449
Fragments arranged by size:
1337 1012 553 467 449 356 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 804 1490 1636 804
Size: 686 146 4390
Fragments arranged by size:
4390 686 146
BtgI C'CryG_G
Cuts at: 107 331 1006 1732 1976 2004 107
Size: 224 675 726 244 28 3325
Fragments arranged by size:
3325 726 675 244 224 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 404 3172 3353 3516 404
Size: 2768 181 163 2110
Fragments arranged by size:
2768 2110 181 163
BtsI GCAGTG_nn'
Cuts at: 835 2465 835
Size: 1630 3592
DraI TTT'AAA
Cuts at: 2221 2221
Size: 5222
DrdI GACnn_nn'nnGTC
Cuts at: 2928 3786 2928
Size: 858 4364
EaeI y'GGCC_r
Cuts at: 230 2807 2981 3372 3399 4744 230
Size: 2577 174 391 27 1345 708
Fragments arranged by size:
2577 1345 708 391 174 27
EagI C'GGCC_G
Cuts at: 2807 2807
Size: 5222
EarI CTCTTCn'nnn_
Cuts at: 256 399 1015 1327 1597 3245 3455 256
Size: 143 616 312 270 1648 210 2023
Fragments arranged by size:
2023 1648 616 312 270 210 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1198 3430 3740 3886 5032 127
Size: 1071 2232 310 146 1146 317
Fragments arranged by size:
2232 1146 1071 317 310 146
EcoICRI GAG'CTC
Cuts at: 1269 2234 1269
Size: 965 4257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 648 707 1004 1085 1105 1186 1261 1352
Size: 302 59 297 81 20 81 75 91
Cuts at: 1352 1453 1733 3047 3479 4226 4494 346
Size: 101 280 1314 432 747 268 1074
Fragments arranged by size:
1314 1074 747 432 302 297 280 268
101 91 81 81 75 59 20
EcoNI CCTnn'n_nnAGG
Cuts at: 958 958
Size: 5222
EcoO109I rG'GnC_Cy
Cuts at: 690 1245 1475 1485 1486 1706 2009 2010 2361
Size: 555 230 10 1 220 303 1 351
Cuts at: 2361 690
Size: 3551
Fragments arranged by size:
3551 555 351 303 230 220 10 1
1
EcoRI G'AATT_C
Cuts at: 2226 2226
Size: 5222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3241 3241
Size: 5222
FspI TGC'GCA
Cuts at: 3003 3003
Size: 5222
HaeII r_GCGC'y
Cuts at: 1559 1674 2656 2904 3926 4905 1559
Size: 115 982 248 1022 979 1876
Fragments arranged by size:
1876 1022 982 979 248 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 514 546 565 597 674 700 706
Size: 32 208 32 19 32 77 26 6
Cuts at: 706 732 787 819 1981 2013 3064 3094 3096
Size: 26 55 32 1162 32 1051 30 2
Cuts at: 3096 3126 274
Size: 30 2370
Fragments arranged by size:
2370 1162 1051 208 77 55 32 32
32 32 32 30 30 26 26 19
6 2
HincII GTy'rAC
Cuts at: 630 2261 630
Size: 1631 3591
KasI G'GCGC_C
Cuts at: 1555 2652 2900 1555
Size: 1097 248 3877
Fragments arranged by size:
3877 1097 248
KpnI G_GTAC'C
Cuts at: 2242 2242
Size: 5222
MfeI C'AATT_G
Cuts at: 1859 1859
Size: 5222
MluI A'CGCG_T
Cuts at: 148 4655 148
Size: 4507 715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1794 3893 4077 1794
Size: 2099 184 2939
Fragments arranged by size:
2939 2099 184
MscI TGG'CCA
Cuts at: 2983 2983
Size: 5222
MslI CAynn'nnrTG
Cuts at: 2065 3211 3338 2065
Size: 1146 127 3949
Fragments arranged by size:
3949 1146 127
MspA1I CmG'CkG
Cuts at: 1809 2333 2647 3007 4020 4265 1809
Size: 524 314 360 1013 245 2766
Fragments arranged by size:
2766 1013 524 360 314 245
NaeI GCC'GGC
Cuts at: 992 1612 3403 992
Size: 620 1791 2811
Fragments arranged by size:
2811 1791 620
NarI GG'CG_CC
Cuts at: 1556 2653 2901 1556
Size: 1097 248 3877
Fragments arranged by size:
3877 1097 248
NcoI C'CATG_G
Cuts at: 107 331 1976 107
Size: 224 1645 3353
Fragments arranged by size:
3353 1645 224
NgoMIV G'CCGG_C
Cuts at: 990 1610 3401 990
Size: 620 1791 2811
Fragments arranged by size:
2811 1791 620
NspI r_CATG'y
Cuts at: 2275 2775 3682 2275
Size: 500 907 3815
Fragments arranged by size:
3815 907 500
PasI CC'CwG_GG
Cuts at: 1482 1549 1482
Size: 67 5155
PciI A'CATG_T
Cuts at: 3678 3678
Size: 5222
PfoI T'CCnGG_A
Cuts at: 692 1132 692
Size: 440 4782
PmeI GTTT'AAAC
Cuts at: 2221 2221
Size: 5222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 4361
Size: 32 5190
PpuMI rG'GwC_Cy
Cuts at: 690 1475 690
Size: 785 4437
PshAI GACnn'nnGTC
Cuts at: 188 1722 188
Size: 1534 3688
PspOMI G'GGCC_C
Cuts at: 783 1485 1706 2009 783
Size: 702 221 303 3996
Fragments arranged by size:
3996 702 303 221
PstI C_TGCA'G
Cuts at: 197 842 917 1160 2166 2269 2954 197
Size: 645 75 243 1006 103 685 2465
Fragments arranged by size:
2465 1006 685 645 243 103 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5222
PvuII CAG'CTG
Cuts at: 1809 2647 3007 1809
Size: 838 360 4024
Fragments arranged by size:
4024 838 360
RsrII CG'GwC_CG
Cuts at: 3417 3417
Size: 5222
SacI G_AGCT'C
Cuts at: 1271 2236 1271
Size: 965 4257
SalI G'TCGA_C
Cuts at: 2259 2259
Size: 5222
SapI GCTCTTCn'nnn_
Cuts at: 256 399 1015 1597 3245 3455 256
Size: 143 616 582 1648 210 2023
Fragments arranged by size:
2023 1648 616 582 210 143
SbfI CC_TGCA'GG
Cuts at: 2269 2269
Size: 5222
ScaI AGT'ACT
Cuts at: 1187 4739 1187
Size: 3552 1670
SfcI C'TryA_G
Cuts at: 33 193 838 851 913 1156 2162 2265 2950
Size: 160 645 13 62 243 1006 103 685
Cuts at: 2950 3943 4134 33
Size: 993 191 1121
Fragments arranged by size:
1121 1006 993 685 645 243 191 160
103 62 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 2182 2182
Size: 5222
SfoI GGC'GCC
Cuts at: 1557 2654 2902 1557
Size: 1097 248 3877
Fragments arranged by size:
3877 1097 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5222
SmaI CCC'GGG
Cuts at: 2015 2244 2015
Size: 229 4993
SmlI C'TyrA_G
Cuts at: 1270 2377 3292 3784 4046 1270
Size: 1107 915 492 262 2446
Fragments arranged by size:
2446 1107 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5222
SphI G_CATG'C
Cuts at: 2275 2775 2275
Size: 500 4722
StuI AGG'CCT
Cuts at: 640 2185 640
Size: 1545 3677
StyI C'CwwG_G
Cuts at: 107 331 491 1559 1976 2356 2710 107
Size: 224 160 1068 417 380 354 2619
Fragments arranged by size:
2619 1068 417 380 354 224 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1841 1880 2805 2938 3397 3606 1841
Size: 39 925 133 459 209 3457
Fragments arranged by size:
3457 925 459 209 133 39
TatI w'GTAC_w
Cuts at: 1185 4737 1185
Size: 3552 1670
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 494 526 2170 2202 4739 4771 494
Size: 32 1644 32 2537 32 945
Fragments arranged by size:
2537 1644 945 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 486 708 1995 3019 486
Size: 222 1287 1024 2689
Fragments arranged by size:
2689 1287 1024 222
XbaI T'CTAG_A
Cuts at: 2253 2253
Size: 5222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1959 2717 1959
Size: 758 4464
XmaI C'CCGG_G
Cuts at: 2013 2242 2013
Size: 229 4993
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BfrBI BmtI BsgI BsiWI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI