Restriction map for
pF1KB6175
(Circular) (Six-base) MAPSORT of: pF1KB6175.seq Check: 467 from: 1 to: 5072
pF1KB6175 5072 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 608 1361 608
Size: 753 4319
AccI GT'mk_AC
Cuts at: 2110 2110
Size: 5072
Acc65I G'GTAC_C
Cuts at: 2088 2088
Size: 5072
AclI AA'CG_TT
Cuts at: 4912 4912
Size: 5072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 534 751 835 1598 2897 3329 4076 4344 534
Size: 217 84 763 1299 432 747 268 1262
Fragments arranged by size:
1299 1262 763 747 432 268 217 84
AfeI AGC'GCT
Cuts at: 643 1084 643
Size: 441 4631
AflIII A'CryG_T
Cuts at: 1877 2424 3528 4505 1877
Size: 547 1104 977 2444
Fragments arranged by size:
2444 1104 977 547
AgeI A'CCGG_T
Cuts at: 3460 3460
Size: 5072
AleI CACnn'nnGTG
Cuts at: 712 1880 1909 712
Size: 1168 29 3875
Fragments arranged by size:
3875 1168 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 730 1848 730
Size: 1118 3954
AlwNI CAG_nnn'CTG
Cuts at: 600 912 1814 1893 2377 3944 600
Size: 312 902 79 484 1567 1728
Fragments arranged by size:
1728 1567 902 484 312 79
ApaI G_GGCC'C
Cuts at: 621 1450 1698 1903 1963 621
Size: 829 248 205 60 3730
Fragments arranged by size:
3730 829 248 205 60
ApaLI G'TGCA_C
Cuts at: 527 534 857 3842 527
Size: 7 323 2985 1757
Fragments arranged by size:
2985 1757 323 7
ApoI r'AATT_y
Cuts at: 2076 4425 4681 2076
Size: 2349 256 2467
Fragments arranged by size:
2467 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5072
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
AvaI C'yCGr_G
Cuts at: 150 233 1039 1193 2092 150
Size: 83 806 154 899 3130
Fragments arranged by size:
3130 899 806 154 83
BamHI G'GATC_C
Cuts at: 154 2097 154
Size: 1943 3129
BanI G'GyrC_C
Cuts at: 1227 1373 1926 2088 2502 2750 2785 1227
Size: 146 553 162 414 248 35 3514
Fragments arranged by size:
3514 553 414 248 162 146 35
BanII G_rGCy'C
Cuts at: 621 848 1400 1450 1664 1698 1903 1963 2086
Size: 227 552 50 214 34 205 60 123
Cuts at: 2086 3116 621
Size: 1030 2577
Fragments arranged by size:
2577 1030 552 227 214 205 123 60
50 34
BbeI G_GCGC'C
Cuts at: 2506 2754 2506
Size: 248 4824
BbsI GAAGACnn'nnnn_
Cuts at: 322 1348 322
Size: 1026 4046
BbvCI CC'TCA_GC
Cuts at: 285 1048 1502 285
Size: 763 454 3855
Fragments arranged by size:
3855 763 454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5038
BciVI GTATCCnnnnn_n'
Cuts at: 2970 3731 5072 2970
Size: 761 1341 2970
Fragments arranged by size:
2970 1341 761
BclI T'GATC_A
Cuts at: 2592 2592
Size: 5072
BglI GCCn_nnn'nGGC
Cuts at: 112 616 1898 1915 2042 112
Size: 504 1282 17 127 3142
Fragments arranged by size:
3142 1282 504 127 17
BglII A'GATC_T
Cuts at: 2587 4624 2587
Size: 2037 3035
BlpI GC'TnA_GC
Cuts at: 628 2184 628
Size: 1556 3516
Bme1580I G_kGCm'C
Cuts at: 531 538 621 861 1450 1698 1903 1963 2063
Size: 7 83 240 589 248 205 60 100
Cuts at: 2063 2697 2790 3846 531
Size: 634 93 1056 1757
Fragments arranged by size:
1757 1056 634 589 248 240 205 100
93 83 60 7
BmgBI CAC'GTC
Cuts at: 1512 1805 1512
Size: 293 4779
BmrI ACTGGGnnnn_n'
Cuts at: 256 879 1416 1869 2284 2365 2700 4844 256
Size: 623 537 453 415 81 335 2144 484
Fragments arranged by size:
2144 623 537 484 453 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3439 3439
Size: 5072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 1159 1587 1746 214
Size: 945 428 159 3540
Fragments arranged by size:
3540 945 428 159
Bpu10I CC'TnA_GC
Cuts at: 278 285 1048 1502 278
Size: 7 763 454 3848
Fragments arranged by size:
3848 763 454 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 2248 3127 3619 3917 622
Size: 1626 879 492 298 1777
Fragments arranged by size:
1777 1626 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4776 4776
Size: 5072
BsaAI yAC'GTr
Cuts at: 1551 1878 2425 1551
Size: 327 547 4198
Fragments arranged by size:
4198 547 327
BsaBI GATnn'nnATC
Cuts at: 6 2586 4623 6
Size: 2580 2037 455
Fragments arranged by size:
2580 2037 455
BsaHI Gr'CG_yC
Cuts at: 877 920 991 1427 1730 2503 2751 3453 4949
Size: 43 71 436 303 773 248 702 1496
Cuts at: 4949 877
Size: 1000
Fragments arranged by size:
1496 1000 773 702 436 303 248 71
43
BsaWI w'CCGG_w
Cuts at: 2261 2782 3460 3734 3881 4292 2261
Size: 521 678 274 147 411 3041
Fragments arranged by size:
3041 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 763 921 936 999 1408 763
Size: 158 15 63 409 4427
Fragments arranged by size:
4427 409 158 63 15
BseYI C'CCAG_C
Cuts at: 255 303 340 606 717 896 1079 1093 1990
Size: 48 37 266 111 179 183 14 897
Cuts at: 1990 2032 2497 3255 3832 4328 255
Size: 42 465 758 577 496 999
Fragments arranged by size:
999 897 758 577 496 465 266 183
179 111 48 42 37 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1018 1827 835
Size: 183 809 4080
Fragments arranged by size:
4080 809 183
BsiEI CG_ry'CG
Cuts at: 104 447 746 796 1219 1389 2614 2660 3868
Size: 343 299 50 423 170 1225 46 1208
Cuts at: 3868 104
Size: 1308
Fragments arranged by size:
1308 1225 1208 423 343 299 170 50
46
BsiHKAI G_wGCw'C
Cuts at: 531 538 848 861 1046 1400 1664 2086 2864
Size: 7 310 13 185 354 264 422 778
Cuts at: 2864 3054 3846 531
Size: 190 792 1757
Fragments arranged by size:
1757 792 778 422 354 310 264 190
185 13 7
BsmBI CGTCTCn'nnnn_
Cuts at: 1795 1795
Size: 5072
Bsp1286I G_dGCh'C
Cuts at: 531 538 621 848 861 1046 1400 1450 1664
Size: 7 83 227 13 185 354 50 214
Cuts at: 1664 1698 1903 1963 2063 2086 2697 2790 2864
Size: 34 205 60 100 23 611 93 74
Cuts at: 2864 3054 3116 3846 531
Size: 190 62 730 1757
Fragments arranged by size:
1757 730 611 354 227 214 205 190
185 100 93 83 74 62 60 50
34 23 13 7
BspEI T'CCGG_A
Cuts at: 4292 4292
Size: 5072
BspHI T'CATG_A
Cuts at: 4248 4248
Size: 5072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 608 833 1361 2122 2336 2638 3019 608
Size: 225 528 761 214 302 381 2661
Fragments arranged by size:
2661 761 528 381 302 225 214
BsrBI CCG'CTC
Cuts at: 50 200 1453 1692 3364 3418 4902 5067 50
Size: 150 1253 239 1672 54 1484 165 55
Fragments arranged by size:
1672 1484 1253 239 165 150 55 54
BsrDI GCAATG_nn'
Cuts at: 561 2984 561
Size: 2423 2649
BsrFI r'CCGG_y
Cuts at: 111 217 1486 1543 1999 3070 3251 3460 111
Size: 106 1269 57 456 1071 181 209 1723
Fragments arranged by size:
1723 1269 1071 456 209 181 106 57
BssSI C'ACGA_G
Cuts at: 1657 3343 3701 1657
Size: 1686 358 3028
Fragments arranged by size:
3028 1686 358
BstAPI GCAn_nnn'nTGC
Cuts at: 189 912 1814 2449 189
Size: 723 902 635 2812
Fragments arranged by size:
2812 902 723 635
BstBI TT'CG_AA
Cuts at: 3433 3433
Size: 5072
BstXI CCAn_nnnn'nTGG
Cuts at: 1882 1911 1882
Size: 29 5043
BstYI r'GATC_y
Cuts at: 1 154 2097 2564 2587 2922 3168 4180 4266
Size: 153 1943 467 23 335 246 1012 86
Cuts at: 4266 4278 4624 1
Size: 12 346 449
Fragments arranged by size:
1943 1012 467 449 346 335 246 153
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1556 1556
Size: 5072
BtgI C'CryG_G
Cuts at: 441 1151 1460 1670 1910 441
Size: 710 309 210 240 3603
Fragments arranged by size:
3603 710 309 240 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 304 3022 3203 3366 304
Size: 2718 181 163 2010
Fragments arranged by size:
2718 2010 181 163
BtsI GCAGTG_nn'
Cuts at: 655 2315 655
Size: 1660 3412
DraI TTT'AAA
Cuts at: 2071 2071
Size: 5072
DraIII CAC_nnn'GTG
Cuts at: 189 1878 189
Size: 1689 3383
DrdI GACnn_nn'nnGTC
Cuts at: 2778 3636 2778
Size: 858 4214
EaeI y'GGCC_r
Cuts at: 444 563 793 866 1057 1216 1700 1884 1916
Size: 119 230 73 191 159 484 184 32
Cuts at: 1916 2657 2831 3222 3249 4594 444
Size: 741 174 391 27 1345 922
Fragments arranged by size:
1345 922 741 484 391 230 191 184
174 159 119 73 32 27
EagI C'GGCC_G
Cuts at: 444 793 1216 2657 444
Size: 349 423 1441 2859
Fragments arranged by size:
2859 1441 423 349
EarI CTCTTCn'nnn_
Cuts at: 1283 1574 3095 3305 1283
Size: 291 1521 210 3050
Fragments arranged by size:
3050 1521 291 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1473 1803 3280 3590 3736 4882 1473
Size: 330 1477 310 146 1146 1663
Fragments arranged by size:
1663 1477 1146 330 310 146
EcoICRI GAG'CTC
Cuts at: 846 1398 1662 2084 846
Size: 552 264 422 3834
Fragments arranged by size:
3834 552 422 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 534 751 835 1159 1587 1598 1746 2897
Size: 320 217 84 324 428 11 148 1151
Cuts at: 2897 3329 4076 4344 214
Size: 432 747 268 942
Fragments arranged by size:
1151 942 747 432 428 324 320 268
217 148 84 11
EcoNI CCTnn'n_nnAGG
Cuts at: 1702 1702
Size: 5072
EcoO109I rG'GnC_Cy
Cuts at: 238 617 1235 1446 1447 1498 1535 1695 1838
Size: 379 618 211 1 51 37 160 143
Cuts at: 1838 1899 1900 1960 2011 2211 238
Size: 61 1 60 51 200 3099
Fragments arranged by size:
3099 618 379 211 200 160 143 61
60 51 51 37 1 1
EcoRI G'AATT_C
Cuts at: 2076 2076
Size: 5072
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3091 3091
Size: 5072
FspI TGC'GCA
Cuts at: 706 1369 2853 706
Size: 663 1484 2925
Fragments arranged by size:
2925 1484 663
FspAI rTGC'GCAy
Cuts at: 706 706
Size: 5072
HaeII r_GCGC'y
Cuts at: 645 929 1086 1212 1353 1419 2506 2754 3776
Size: 284 157 126 141 66 1087 248 1022
Cuts at: 3776 4755 645
Size: 979 962
Fragments arranged by size:
1087 1022 979 962 284 248 157 141
126 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1000 1032 2914 2944 2946 2976 1000
Size: 32 1882 30 2 30 3096
Fragments arranged by size:
3096 1882 32 30 30 2
HincII GTy'rAC
Cuts at: 969 2111 969
Size: 1142 3930
HindIII A'AGCT_T
Cuts at: 763 763
Size: 5072
KasI G'GCGC_C
Cuts at: 2502 2750 2502
Size: 248 4824
KpnI G_GTAC'C
Cuts at: 2092 2092
Size: 5072
MluI A'CGCG_T
Cuts at: 4505 4505
Size: 5072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1595 1616 3743 3927 1595
Size: 21 2127 184 2740
Fragments arranged by size:
2740 2127 184 21
MscI TGG'CCA
Cuts at: 868 1886 2833 868
Size: 1018 947 3107
Fragments arranged by size:
3107 1018 947
MslI CAynn'nnrTG
Cuts at: 712 1150 1330 1880 1909 3061 3188 712
Size: 438 180 550 29 1152 127 2596
Fragments arranged by size:
2596 1152 550 438 180 127 29
MspA1I CmG'CkG
Cuts at: 307 840 1032 1462 1811 2183 2497 2857 3870
Size: 533 192 430 349 372 314 360 1013
Cuts at: 3870 4115 307
Size: 245 1264
Fragments arranged by size:
1264 1013 533 430 372 360 349 314
245 192
NaeI GCC'GGC
Cuts at: 113 219 2001 3253 113
Size: 106 1782 1252 1932
Fragments arranged by size:
1932 1782 1252 106
NarI GG'CG_CC
Cuts at: 2503 2751 2503
Size: 248 4824
NcoI C'CATG_G
Cuts at: 1151 1151
Size: 5072
NgoMIV G'CCGG_C
Cuts at: 111 217 1999 3251 111
Size: 106 1782 1252 1932
Fragments arranged by size:
1932 1782 1252 106
NruI TCG'CGA
Cuts at: 1520 1520
Size: 5072
NspI r_CATG'y
Cuts at: 348 2125 2625 3532 348
Size: 1777 500 907 1888
Fragments arranged by size:
1888 1777 907 500
PasI CC'CwG_GG
Cuts at: 372 372
Size: 5072
PciI A'CATG_T
Cuts at: 3528 3528
Size: 5072
PflMI CCAn_nnn'nTGG
Cuts at: 691 891 1956 691
Size: 200 1065 3807
Fragments arranged by size:
3807 1065 200
PmeI GTTT'AAAC
Cuts at: 2071 2071
Size: 5072
PmlI CAC'GTG
Cuts at: 1878 1878
Size: 5072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 5040
PpuMI rG'GwC_Cy
Cuts at: 238 1235 1535 1838 238
Size: 997 300 303 3472
Fragments arranged by size:
3472 997 303 300
PspOMI G'GGCC_C
Cuts at: 617 1446 1694 1899 1959 617
Size: 829 248 205 60 3730
Fragments arranged by size:
3730 829 248 205 60
PstI C_TGCA'G
Cuts at: 662 839 1625 1898 2119 2804 662
Size: 177 786 273 221 685 2930
Fragments arranged by size:
2930 786 685 273 221 177
PvuI CG_AT'CG
Cuts at: 104 746 104
Size: 642 4430
PvuII CAG'CTG
Cuts at: 2497 2857 2497
Size: 360 4712
RsrII CG'GwC_CG
Cuts at: 3267 3267
Size: 5072
SacI G_AGCT'C
Cuts at: 848 1400 1664 2086 848
Size: 552 264 422 3834
Fragments arranged by size:
3834 552 422 264
SacII CC_GC'GG
Cuts at: 1463 1463
Size: 5072
SalI G'TCGA_C
Cuts at: 2109 2109
Size: 5072
SanDI GG'GwC_CC
Cuts at: 238 1235 238
Size: 997 4075
SapI GCTCTTCn'nnn_
Cuts at: 3095 3305 3095
Size: 210 4862
SbfI CC_TGCA'GG
Cuts at: 1898 2119 1898
Size: 221 4851
ScaI AGT'ACT
Cuts at: 889 4589 889
Size: 3700 1372
SfcI C'TryA_G
Cuts at: 33 658 835 1086 1419 1621 1894 2115 2800
Size: 625 177 251 333 202 273 221 685
Cuts at: 2800 3793 3984 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 625 333 273 251 221
202 191 177
SfoI GGC'GCC
Cuts at: 2504 2752 2504
Size: 248 4824
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
SmaI CCC'GGG
Cuts at: 235 1195 2094 235
Size: 960 899 3213
Fragments arranged by size:
3213 960 899
SmlI C'TyrA_G
Cuts at: 637 2227 3142 3634 3896 637
Size: 1590 915 492 262 1813
Fragments arranged by size:
1813 1590 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5072
SphI G_CATG'C
Cuts at: 348 2125 2625 348
Size: 1777 500 2795
Fragments arranged by size:
2795 1777 500
StuI AGG'CCT
Cuts at: 283 1720 283
Size: 1437 3635
StyI C'CwwG_G
Cuts at: 1151 2206 2560 1151
Size: 1055 354 3663
Fragments arranged by size:
3663 1055 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 555 1199 1966 2655 2788 3247 3456 555
Size: 644 767 689 133 459 209 2171
Fragments arranged by size:
2171 767 689 644 459 209 133
TatI w'GTAC_w
Cuts at: 887 4587 887
Size: 3700 1372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1782 1814 4589 4621 1782
Size: 32 2775 32 2233
Fragments arranged by size:
2775 2233 32 32
Tth111I GACn'n_nGTC
Cuts at: 2869 2869
Size: 5072
XbaI T'CTAG_A
Cuts at: 2103 2103
Size: 5072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1911 2567 1911
Size: 656 4416
XmaI C'CCGG_G
Cuts at: 233 1193 2092 233
Size: 960 899 3213
Fragments arranged by size:
3213 960 899
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BmtI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI CspCI EcoRV FseI HpaI MfeI NdeI NheI
NotI NsiI PacI PfoI PshAI PsiI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI